Gateway vectors for the production of combinatorially-tagged His 6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli

Protein Science

Volumn 14, Issue 12, 2005, Pages 2964-2971

  Nallamsetty, Sreedevi ^a   Austin, Brian P ^a   Penrose, Kerri J ^a   Waugh, David S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Expression vector; Fusion protein; Fusion tag; Gateway cloning; IMAC; Immobilized metal affinity chromatography; Maltose binding protein; Structural genomics; TEV protease

Indexed keywords

HISTIDINE DERIVATIVE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN;

ARTICLE; COMBINATORIAL CHEMISTRY; CONFORMATION; CYTOPLASM; DISULFIDE BOND; ESCHERICHIA COLI; EXPRESSION VECTOR; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN PURIFICATION; SOLUBILITY; STRUCTURAL GENOMICS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CARRIER PROTEINS; CYTOPLASM; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HISTIDINE; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PERIPLASM; RECOMBINANT FUSION PROTEINS; RIBONUCLEASES; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TOBACCO ETCH VIRUS;

EID: 28844475180     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.051718605     Document Type: Article

References (27)

1. Amann, E., Brosius, J., and Ptashne, M. 1983. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in E. coli. Gene 25: 167-178.
2. Bach, H., Mazor, Y., Shaky, S., Shoham-Lev, A., Berdichevsky, Y., Gutnick, D.L., and Benhar, I. 2001. Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies. J. Mol. Biol. 312: 79-93.
3. Chayen, N.E. 2004. Turning protein crystallization from an art into a science. Curr. Opin. Struct. Biol. 14: 577-583.
4. DelCardayré, S.B., Ribó, M., Yokel, E.M., Quirk, D.J., Rutter, W.J., and Raines, R.T. 1995. Engineering ribonuclease A: Production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 8: 261-273.
5. Evdokimov, A.G., Tropea, J.E., Routzahn, K.M., and Waugh, D.S. 2002. Three-dimensional structure of the type III secretion chaperone SycE from Yersinia pestis. Acta Crystallogr. D Biol. Crystallogr. 58: 398-406.
6. Fox, J.D. and Waugh, D.S. 2003. Maltose-binding protein as a solubility enhancer. Methods Mol. Biol. 205: 99-117.
7. Fox, J.D., Routzahn, K.M, Bucher, M.H., and Waugh, D.S. 2003. Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. FEBS Lett. 537: 53-77.
8. Giuliani, C.D., Iemma, M.R., Bondioli, A.C., Souza, D.H., Ferreira, L.L., Amaral, A.C., Salvini, T.F., and Selistre-de-Araujo, H.S. 2001. Expression of an active recombinant lysine 49 phospholipase A(2) myotozin as a fusion protein in bacteria. Toxicon 39: 1595-1600.
9. Goh, L.L., Loke, P., Singh, M., and Sim, T.S. 2003. Soluble expression of a functionally active Plasmodium falciparum falcipain-2 fused to maltose-binding protein in Escherichia coli. Prot. Expr. Purif. 32: 194-201.
10. Hammarström, M., Hellgren, N., Van den Berg, S., Berglund, H., and Härd, T. 2002. Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli. Protein Sci. 11: 313-321.
11. Huys, I., Dyason, K., Waelkens, E., Verdonck, F., Van Zyl, J., Du Plessis, J., Müller, G.J., Van der Walt, J., Clynen, E., Schoofs, L., et al. 2002. Purification, characterization and biosynthesis of parabutoxin 3, a component of Parabuthus transvaalicus venom. Eur. J. Biochem. 269: 1854-1865.
12. Kapust, R.B. and Waugh, D.S. 1999. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 8: 1668-1674.
13. Kapust, R.B., Tözsér, J., Fox, J.D., Anderson, D.E., Cherry, S., Copeland, T.D., and Waugh, D.S. 2001. Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng. 14: 993-1000.
14. Klink, T.A., Woycechowsky, K.J., Taylor, K.M., and Raines, R.T. 2000. Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A. Eur. J. Biochem. 267: 566-572.
15. Muramatsu, H., Yoshikawa, K., Hayashi, T., Takasu, S., Kawada, Y., Uchida, K., Sato, S., Takahashi, T., Saga, S., and Ueda, R. 2005. Production and characterization of an active single-chain variable fragment antibody recognizing CD25. Cancer Lett. 225: 225-236.
16. Nallamsetty, S., Kapust, R.B., Tözsér, J., Cherry, S., Tropea, J.E., Copeland, T.D., and Waugh, D.S. 2004. Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Protein Expr. Purif. 38: 108-115.
17. Neu, H.C. and Chou, J. 1967. Release of surface enzymes in Enterobacteriaceae by osmotic shock. J. Bacteriol. 94: 1934-1945.
18. Nomine, Y., Ristriani, T., Laurent, C., Lefevre, J.F., Weiss, E., and Trave, G. 2001. A strategy for optimizing the monodispersity of fusion proteins: Application to purification of recombinant HPV E6 oncoprotein, Protein Eng. 14: 297-305.
19. Planson, A.G., Guijarro, J.I., Goldberg, M.E., and Chaffotte, A.F. 2003. Assistance of maltose binding protein to the in vivo folding of the disulfide-rich C-terminal fragment from Plasmodium falciparum merozoite surface protein 1 in Escherichia coli. Biochemistry 42: 13202-13211.
20. 6-tag and maltose-binding- protein double-affinity fusion system. Protein Expr. Purif. 10: 309-319.
21. Riggs, P. 2000. Expression and purification of recombinant proteins by fusion to maltose-binding protein. Mol. Biotechnol. 15: 51-63.
22. Routzahn, K.M. and Waugh, D.S. 2002. Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins. J. Struct. Funct. Genomics 2: 83-92.
23. Shih, Y.P., Kung, W.M., Chen, J.C., Yeh, C.H., Wang, A.H.J., and Wang, T.F. 2002. High-throughput screening of soluble recombinant proteins. Protein Sci. 11: 1714-1719.
24. Stevens, R.C. 2000. Design of high-throughput methods of protein production for structural biology. Structure 8: R177-R185.
25. 6-MBP affinity tag. Methods Mol. Biol. (in press).
26. Uchida, T. and Egami, F. 1966. A general ribonuclease assay. In Proceedings in nucleic acid research (eds. G.L. Cantoni and D.R. Davis), pp. 46-55. Harper & Row, New York and London.
27. Waugh, D.S. 2005. Making the most of affinity tags. Trends Biotechnol. 23: 316-320.