Expressivity tag: A novel tool for increased expression in Escherichia coli

Journal of Biotechnology

Volumn 155, Issue 3, 2011, Pages 275-283

  Hansted, Jon Gade ^a   Pietikäinen, Laura ^a   Hög, Friederike ^a   Sperling Petersen, Hans Uffe ^a   Mortensen, Kim Kusk ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

Escherichia coli; Expressivity tag; Heterologous protein expression; IF2; Initiation factor 2

Indexed keywords

AFFINITY TAGS; CODON USAGE; COMMON PROBLEMS; EXPRESSION IN ESCHERICHIA COLI; EXPRESSION LEVELS; EXPRESSIVITY TAG; FUSED GENES; FUSION PARTNERS; FUSION TAG; GENE DISPLAY; GFP EXPRESSION; GFP REPORTER; HETEROLOGOUS PROTEINS; IF2; INITIATION FACTORS; PROTEIN EXPRESSIONS; SDS-PAGE; SINGLE-CHAIN ANTIBODIES; STREPTAVIDIN;

ESCHERICHIA COLI; OPTIMIZATION; PROTEINS;

GENE EXPRESSION;

GREEN FLUORESCENT PROTEIN; INITIATION FACTOR; PROTEIN TAG; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; STREPTAVIDIN;

ARTICLE; BACTERIAL GENE; BIOTECHNOLOGICAL PROCEDURES; CODON USAGE; CONTROLLED STUDY; ENERGY; ESCHERICHIA COLI; EXPRESSIVITY TAG; GENE DELETION; GENE FUSION; GENE INSERTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; SOLUBILITY; TRANSLATION INITIATION;

ANIMALS; BIOTECHNOLOGY; CLONING, MOLECULAR; COMPUTER SIMULATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HUMANS; PROTEIN BIOSYNTHESIS; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SINGLE-CHAIN ANTIBODIES; SOLUBILITY; SPECTROMETRY, FLUORESCENCE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STATISTICS, NONPARAMETRIC; THERMODYNAMICS;

ESCHERICHIA COLI;

EID: 80052364548     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.07.013     Document Type: Article

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