Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coli protein, RpoS

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Park, Jin Seung ^a   Han, Kyung Yeon ^a   Lee, Jong Ho ^a   Song, Jong Am ^a   Ahn, Keum Young ^a   Seo, Hyuk Seong ^a   Sim, Sang Jun ^b   Kim, Seung Wook ^a   Lee, Jeewon ^a  

^a Korea University   (South Korea)

^b Sungkyunkwan University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; ESCHERICHIA COLI; RNA; SOLUBILITY; SYNTHESIS (CHEMICAL);

CYTOPLASM; FUSION EXPRESSION; POLYMERASE SIGMA FACTOR; RECOMBINANT PROTEINS; STRESS RESPONSIVE;

PROTEINS;

CUTINASE; GUANIDINE; HYBRID PROTEIN; PROTEOME; RECOMBINANT PROTEIN; SIGMA FACTOR RPOS; BACTERIAL PROTEIN; ESCHERICHIA COLI PROTEIN; SIGMA FACTOR; SIGMA FACTOR KATF PROTEIN, BACTERIA;

ARTICLE; CONTROLLED STUDY; CYTOPLASM; DATA MINING; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HETEROLOGOUS EXPRESSION; MUTANT; NONHUMAN; PROTEIN AGGREGATION; PROTEIN SYNTHESIS; PROTEOMICS; PSEUDOMONAS PUTIDA; SOLUBILITY; STRESS; TWO DIMENSIONAL GEL ELECTROPHORESIS; DIMERIZATION; GENETICS; METABOLISM; METHODOLOGY; OXIDATIVE STRESS; PHYSIOLOGY; PROTEIN BINDING; PROTEIN ENGINEERING;

ESCHERICHIA COLI; PSEUDOMONAS PUTIDA;

BACTERIAL PROTEINS; DIMERIZATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; OXIDATIVE STRESS; PROTEIN BINDING; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SIGMA FACTOR; SOLUBILITY;

EID: 41549151572     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-15     Document Type: Article

References (37)

1. Finley JB Qui SH Luan CH Luo M Structural genomics for Caenorhabditis elegans: High throughput protein expression analysis Protein Expr Purif 2004, 34:49-55. 10.1016/j.pep.2003.11.026 14766299
2. Himanen JP Rajashankar KR Lackmann M Cowan CA Henkemeyer M Nikolov DB Crystal structure of an Eph receptor-ephrin complex Nature 2001, 414:933-938. 10.1038/414933a 11780069
3. de Marco A Deuerling E Mogk A Tomoyasu T Bukau B Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli BMC Biotechnol 2007, 7:32. 1904446 17565681 10.1186/ 1472-6750-7-32
4. Qiu J Swartz JR Georgiou G Expression of active human tissue-type plasminogen activator in Escherichia coli Appl Environ Microbiol 1998, 64:4891-4896. 90939 9835579
5. Tan WS Dyson MR Murray K Hepatitis B virus core antigen: Enhancement of its production in Escherichia coli, and interaction of the core particles with the viral surface antigen Biol Chem 2003, 384:363-371. 10.1515/BC.2003.042 12715887
6. Braun P Hu Y Shen B Halleck A Koundinya M Harlow E LaBaer J Proteome-scale purification of human proteins from bacteria Proc Natl Acad Sci USA 2002, 99:2654-2659. 122403 11880620 10.1073/pnas.042684199
7. Hammarstrom M Hellgren N van Den Berg S Berglund H Hard T Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli Protein Sci 2002, 11:313-321. 10.1110/ps.22102 11790841
8. Bedouelle H Duplay P Production in Escherichia coli and one-step purification of bifunctional hybrid proteins which bind maltose. Export of the Klenow polymerase into the periplasmic space Eur J Biochem 1998, 171:541-549. 10.1111/j.1432-1033.1988.tb13823.x
9. LaVallie ER DiBlasio EA Kovacic S Grant KL Schendel PF McCoy JM A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm Biotechnology (N. Y.) 1993, 11:187-193. 10.1038/nbt0293-187 7763371
10. Ahn JY Choi H Kim YH Han KY Park JS Han SS Lee J Heterologous gene expression using self-assembled supra-molecules with high affinity for HSP70 chaperone Nucleic Acids Res 2005, 33:3751-3762. 1174908 16006621 10.1093/nar/gki692
11. Smith DB Johnson KS Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase Gene 1988, 67:31-40. 10.1016/0378-1119(88)90005-4 3047011
12. Hammarstrom M Woestenenk EA Hellgren N Hard T Berglund H Effect of N-terminal solubility enhancing fusion proteins on yield of purified target protein J Struct Funct Genomics 2006, 7:1-14. 10.1007/ s10969-005-9003-7 16850178
13. Sakane I Hongo K Motojima F Murayama S Mizobata T Kawata Y Structural stability of covalently linked GroES heptamer: Advantages in the formation of oligomeric structure J Mol Biol 2007, 367:1171-1185. 10.1016/j.jmb.2007.01.037 17303164
14. Eaglestone SS Ruddock LW Cox BS Tuite MF Guanidine hydrochloride blocks a critical step in the propagation of the prion-like determinant [PSI(+)] of Saccharomyces cerevisiae Proc Natl Acad Sci USA 2000, 97:240-244. 26647 10618402 10.1073/pnas.97.1.240
15. Park JS Ahn JY Lee SH Lee H Han KY Seo HS Ahn KY Min BH Sim SJ Choi IS Kim YH Lee J Enhanced stability of heterologous proteins by supramolecular self-assembly Appl Microbiol Biotechnol 2007, 75:347-355. 10.1007/s00253-006-0826-3 17546471
16. Weber A Kögl SA Jung K Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia col J Bacteriol 2006, 188:7165-7175. 1636219 17015655 10.1128/JB.00508-06
17. Chen YC Chen LA Chen SJ Chang MC Chen TL A modified osmotic shock for periplasmic release of a recombinant creatinase from Escherichia coli Biochem Eng J 2004, 19:211-215. 10.1016/j.bej.2004.03.001
18. Ewis HE Lu CD Osmotic shock: A mechanosensitive channel blocker can prevent release of cytoplasmic but not periplasmic proteins FEMS Microbial Lett 2005, 253:295-301. 10.1016/j.femsle.2005.09.046
19. Hirsch M Elliott T Stationary-phase regulation of RpoS translation in Escherichia coli J Bacteriol 2005, 187:7204-7213. 1272984 16237004 10.1128/JB.187.21.7204-7213.2005
20. Wei B Shin S LaPorte D Wolfe AJ Romeo T Global regulatory mutations in csr A and rpoS cause severe central carbon stress in Escherichia coli in the presence of acetate J Bacteriol 2000, 182:1632-1640. 94461 10692369 10.1128/JB.182.6.1632-1640.2000
21. Muffler A Barth M Marschall C Hengge-Aronis R Heat shock regulation of sigmaS turnover: A role for DnaK and relationship between stress responses mediated by sigmaS and sigma32 in Escherichia coli J Bacteriol 1997, 179:445-452. 178715 8990297
22. Stenström CM Jin H Major LL Tate WP Isaksson LA Codon bias at the 3'-side of the initiation codon is correlated with translation initiation efficiency in Escherichia col Gene 2001, 263:273-284. 10.1016/ S0378-1119(00)00550-3 11223267
23. Flipsen JAC Appel ACM Van der Hijden HTWM Verrips CT Mechanism of removal of immobilized triacylglycerol by lipolytic enzymes in a sequential launcry wash process Enzyme Microb Technol 1998, 23:274-280. 10.1016/S0141-0229(98)00050-7
24. Murphy CA Cameron JA Huang SJ Vinopal RT Fusarium polycaprolactone depolymerase is cutinase Appl Environ Microbiol 1996, 62:456-460. 167813 8593048
25. Cristina MLC Maria RAB Joaquim MSC Cutinase: From molecular level to bioprocess development Biotechnol Bioeng 1999, 66:17-34. 10.1002/ (SICI)1097-0290(1999)66:1<17::AID-BIT2<3.0.CO;2-F 10556791
26. Kim YH Lee J Ahn JY Gu MB Moon SH Enhanced degradation of an endocrine-disrupting chemical, butyl benzyl phthalate, by Fusarium oxysporum f. sp. pisi cutinase Appl Environ Microbiol 2002, 68:4684-4688. 124131 12200333 10.1128/AEM.68.9.4684-4688.2002
27. Martinez C De Geus P Lauwereys M Matthysens G Cambillau C Fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent Nature 1992, 356:615-618. 10.1038/356615a0 1560844
28. Gerard HC Fett WF Osman SF Moreau RA Evaluation of cutinase activity of various industrial lipases Biotechnol Appl Biochem 1993, 17:181-189.
29. Lin TS Kolattukudy PE Structural studies on cutinase, a glycoprotein containing novel amino acids and glucuronic acid amide at the N terminus Eur J Biochem 1980, 106:341-351. 10.1111/j.1432-1033.1980.tb04580.x 7398618
30. Kim DY Lee J Saraswat V Park YH Glucagon-induced self-association of recombinant proteins in Escherichia coli and affinity purification using a fragment of glucagon receptor Biotechnol Bioeng 2000, 69:418-428. 10.1002/1097-0290(20000820)69:4<418::AID-BIT8<3.0.CO;2-C 10862680
31. Choi H Ahn JY Sim SJ Lee J Glutamate decarboxylase -derived IDDM autoantigens displayed on self-assembled protein nanoparticles Biochem Biophys Res Commun 2005, 327:604-608. 10.1016/j.bbrc.2004.12.046 15629156
32. Kim SW Kim YH Lee J Thermal stability of human ferritin: Concentration dependence and enhanced stability of an N-terminal fusion mutant Biochem Biophys Res Commun 2001, 289:125-129. 10.1006/bbrc.2001.5931 11708788
33. Kim YH Han KY Lee K Heo JH Kang HY Lee J Comparative proteome analysis of Hansenula polymorpha DL1 and A16 Proteomics 2004, 4:2005-2013. 10.1002/pmic.200300739 15221762
34. MS Fit http://prospector.ucsf.edu/
35. ExPASy Proteomics Server http://www.expasy.org/
36. Compute pI/Mw tool http://www.expasy.org/tools/pi_tool.html
37. Aldente: Peptide mass fingerprinting tool http://au.expasy.org/tools/ aldente/