Organic toxins as tools to understand ion channel mechanisms and structure

Current Topics in Medicinal Chemistry

Volumn 15, Issue 7, 2015, Pages 581-603

  Morales Lázaro, Sara Luz ^a   Hernández García, Enrique ^a   Serrano Flores, Barbara ^a   Rosenbaum, Tamara ^a  

^a INSTITUTO DE FISIOLOGÍA CELULAR   (Mexico)

Author keywords

Gating modifiers; Ion channels; Ligand gated channels; Pore blockers; Structure function; Toxin; Voltage gated channel

Indexed keywords

BLOCKING AGENT; CALCIUM CHANNEL BLOCKING AGENT; CHOLINERGIC RECEPTOR; CIGUATOXIN; CONE SNAIL TOXIN; ION CHANNEL; ION TRANSPORT AFFECTING AGENT; KV CHANNEL INTERACTING PROTEIN; LIGAND GATED ION CHANNEL; MECHANO SENSITIVE ION CHANNEL; POTASSIUM CHANNEL; RESINIFERATOXIN; SCORPION VENOM; SEA ANEMONE TOXIN; SNAKE VENOM; SODIUM CHANNEL; SODIUM CHANNEL BLOCKING AGENT; SPIDER VENOM; TETRODOTOXIN; TOXIN; UNCLASSIFIED DRUG; VANILLOID RECEPTOR 1; VANILLOID RECEPTOR ANTAGONIST; VENOM; VOLTAGE GATED ION CHANNEL; LIGAND; PROTEIN SUBUNIT;

ARTICLE; BIOCHEMISTRY; HUMAN; NONHUMAN; PROTEIN FUNCTION; PROTEIN STRUCTURE; ANIMAL; ANTAGONISTS AND INHIBITORS; CHANNEL GATING; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECTS; METABOLISM; PROTEIN CONFORMATION; PROTEIN SUBUNIT; STRUCTURE ACTIVITY RELATION;

ANIMALS; HUMANS; ION CHANNEL GATING; ION CHANNELS; LIGANDS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN SUBUNITS; STRUCTURE-ACTIVITY RELATIONSHIP; TOXINS, BIOLOGICAL;

EID: 84930982023     PISSN: 15680266     EISSN: 18734294     Source Type: Journal    
DOI: 10.2174/1568026615666150217110710     Document Type: Article

References (297)

1. Rodriguez de la Vega R. C.; Merino, E.; Becerril, B.; Possani, L. D.; Novel interactions between K+ channels and scorpion toxins. Trends Pharmacol. Sci., 2003, 24(5), 222-7.
2. Miller, C. An overview of the potassium channel family. Genome. Biol, 2000, 1(4), REVIEWS 0004.
3. Bhave, G.; Zhu, W.; Wang, H.; Brasier, D. J.; Oxford, G. S.; Gereau, R. W.; t. cAMP-dependent protein kinase regulates desensitization of the capsaicin receptor (VR1) by direct phosphorylation. Neuron, 2002, 35(4), 721-31.
4. Lazniewska, J.; Weiss, N. The "Sweet" Side of Ion Channels. Rev. Physiol. Biochem. Pharmacol, 2014; 167: 67-114.
5. Olschewski, A.; Weir, E. K. Redox Regulation of Ion Channels in the Pulmonary Circulation. Antioxid. Redox. Signal., 2014.
6. Gruslova, A.; Semenov, I.; Wang, B. An extracellular domain of the accessory beta1 subunit is required for modulating BK channel voltage sensor and gate. J. Gen. Physiol., 2012, 139(1), 57-67.
7. Goldfarb, M. Voltage-gated sodium channel-associated proteins and alternative mechanisms of inactivation and block. Cell. Mol. Life Sci., 2012, 69(7), 1067-76.
8. Gutman, G. A.; Chandy, K. G.; Grissmer, S.; Lazdunski, M.; McKinnon D.; Pardo L. A.; Robertson G. A.; Rudy B.; Sanguinetti M. C.; Stuhmer W.; Wang X. International Union of Pharmacology. LIII. Nomenclature and molecular relationships of voltagegated potassium channels. Pharmacol. Rev., 2005, 57(4), 473-508.
9. Lee, S. Y.; Lee A.; Chen, J.; MacKinnon, R. Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane. Proc. Natl. Acad. Sci. U S A, 2005, 102(43), 15441-6.
10. Long, S. B.; Campbell, E. B.; Mackinnon, R. Crystal structure of a mammalian voltage-dependent Shaker family K+ channel. Science, 2005, 309(5736), 897-903.
11. Bezanilla, F. The voltage sensor in voltage-dependent ion channels. Physiol. Rev., 2000, 80(2), 555-92.
12. Elliott, D. J.; Neale, E. J.; Aziz, Q.; Dunham, J. P.; Munsey, T. S.; Hunter, M.; Sivaprasadarao, A. Molecular mechanism of voltage sensor movements in a potassium channel. EMBO J., 2004, 23(24), 4717-26.
13. Doyle, D. A.; Morais, Cabral, J.; Pfuetzner, R. A.; Kuo, A.; Gulbis, J. M.; Cohen, S. L.; Chait, B. T.; MacKinnon, R. The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science, 1998, 280(5360), 69-77.
14. Zhou, Y.; Morais-Cabral, J. H.; Kaufman, A.; MacKinnon, R. Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2. 0 A resolution. Nature, 2001, 414(6859), 43-8.
15. Corry, B.; Allen, T. W.; Kuyucak, S.; Chung, S. H. Mechanisms of permeation and selectivity in calcium channels. Biophys. J., 2001, 80(1), 195-214.
16. Furini, S.; Domene, C. On conduction in a bacterial sodium channel. PLoS Comput. Biol., 2012, 8(4), e1002476.
17. Catterall, W. A. Structure and function of voltage-gated sodium and calcium channels. Curr. Opin. Neurobiol., 1991, 1(1), 5-13.
18. Catterall, W. A. Structure and function of voltage-gated ion channels. Annu. Rev. Biochem., 1995, 64, 493-531.
19. Catterall, W. A. Voltage-gated sodium channels at 60: structure, function and pathophysiology. J. Physiol, 2012, 590(Pt 11), 2577-89.
20. Flockerzi, V.; Oeken, H. J.; Hofmann, F.; Pelzer, D.; Cavalie, A.; Trautwein, W. Purified dihydropyridine-binding site from skeletal muscle t-tubules is a functional calcium channel. Nature, 1986, 323(6083), 66-8.
21. Curtis, B. M.; Catterall, W. A. Purification of the calcium antagonist receptor of the voltage-sensitive calcium channel from skeletal muscle transverse tubules. Biochemistry, 1984, 23(10), 2113-8.
22. Hosey, M. M.; Barhanin, J.; Schmid, A.; Vandaele, S.; Ptasienski, J.; O'Callahan, C.; Cooper, C.; Lazdunski, M. Photoaffinity labelling and phosphorylation of a 165 kilodalton peptide associated with dihydropyridine and phenylalkylamine-sensitive calcium channels. Biochem. Biophys. Res. Commun., 1987, 147(3), 1137-45.
23. Leung, A. T.; Imagawa, T.; Campbell, K. P. Structural characterization of the 1, 4-dihydropyridine receptor of the voltage-dependent Ca2+ channel from rabbit skeletal muscle. Evidence for two distinct high molecular weight subunits. J. Biol. Chem., 1987, 262(17), 7943-6.
24. Striessnig, J.; Knaus, H. G.; Grabner, M.; Moosburger, K.; Seitz, W.; Lietz, H.; Glossmann, H. Photoaffinity labelling of the phenylalkylamine receptor of the skeletal muscle transverse-tubule calcium channel. FEBS. Lett., 1987, 212(2), 247-53.
25. Takahashi, M.; Seagar, M. J.; Jones, J. F.; Reber, B. F.; Catterall, W. A. Subunit structure of dihydropyridine-sensitive calcium channels from skeletal muscle. Proc. Natl. Acad. Sci. U S A, 1987, 84(15), 5478-82.
26. Liu, H.; De, Waard M.; Scott, V. E.; Gurnett, C. A.; Lennon, V. A.; Campbell, K. P. Identification of three subunits of the high affinity omega-conotoxin MVIIC-sensitive Ca2+ channel. J. Biol. Chem., 1996, 271(23), 13804-10.
27. Martin-Moutot, N.; Leveque, C.; Sato, K.; Kato, R.; Takahashi, M.; Seagar, M. Properties of omega conotoxin MVIIC receptors associated with alpha 1A calcium channel subunits in rat brain. FEBS. Lett., 1995, 366(1), 21-5.
28. McEnery, M. W.; Snowman, A. M.; Snyder, S. H. Evidence for subtypes of the omega-conotoxin GVIA receptor. Identification of the properties intrinsic to the high-affinity receptor. Ann. N. Y. Acad. Sci., 1991, 635, 435-7.
29. Witcher, D. R.; De, Waard M.; Liu, H.; Pragnell, M.; Campbell, K. P. Association of native Ca2+ channel beta subunits with the alpha 1 subunit interaction domain. J. Biol. Chem., 1995, 270(30), 18088-93.
30. Catterall, W. A. Structure and regulation of voltage-gated Ca2+ channels. Annu. Rev. Cell. Dev. Biol., 2000, 16, 521-55.
31. Catterall, W. A.; Striessnig, J.; Snutch, T. P.; Perez-Reyes, E. International Union of Pharmacology. XL. Compendium of voltagegated ion channels: calcium channels. Pharmacol. Rev., 2003, 55, 579-81.
32. Ertel, E. A.; Campbell, K. P.; Harpold, M. M.; Hofmann, F.; Mori, Y.; Perez-Reyes, E.; Schwartz, A.; Snutch, T. P.; Tanabe, T.; Birnbaumer, L.; Tsien, R. W.; Catterall, W. A. Nomenclature of voltage-gated calcium channels. Neuron, 2000, 25(3), 533-5.
33. Tang, L.; Gamal, El-Din T. M.; Payandeh, J.; Martinez, G. Q.; Heard, T. M.; Scheuer, T.; Zheng, N.; Catterall, W. A. Structural basis for Ca2+ selectivity of a voltage-gated calcium channel. Nature, 2014, 505(7481), 56-61.
34. De-la-Rosa, V.; Rangel-Yescas, G. E.; Ladron-de-Guevara, E.; Rosenbaum, T.; Islas, L. D. Coarse architecture of the transient receptor potential vanilloid 1 (TRPV1) ion channel determined by fluorescence resonance energy transfer. J. Biol. Chem., 2013, 288(41), 29506-17.
35. Cao, E.; Liao, M.; Cheng, Y.; Julius, D. TRPV1 structures in distinct conformations reveal activation mechanisms. Nature, 2013, 504(7478), 113-8.
36. Liao, M.; Cao, E.; Julius, D.; Cheng, Y. Structure of the TRPV1 ion channel determined by electron cryo-microscopy. Nature, 2013, 504(7478), 107-12.
37. Wess, J.; Eglen, R. M.; Gautam, D. Muscarinic acetylcholine receptors: mutant mice provide new insights for drug development. Nat. Rev. Drug Discov., 2007, 6(9), 721-33.
38. Albuquerque, E. X.; Barnard, E. A.; Porter, C. W.; Warnick, J. E. The density of acetylcholine receptors and their sensitivity in the postsynaptic membrane of muscle endplates. Proc. Natl. Acad. Sci. U S A, 1974, 71(7), 2818-22.
39. Arroyo-Jim nez, M. M.; Bourgeois, J. P.; Marubio, L. M.; Le, Sourd A. M.; Ottersen, O. P.; Rinvik, E.; Fairen, A.; Changeux, J. P. Ultrastructural localization of the alpha4-subunit of the neuronal acetylcholine nicotinic receptor in the rat substantia nigra. J. Neurosci., 1999, 19(15), 6475-87.
40. Clarke, P. B.; Schwartz, R. D.; Paul, S. M.; Pert, C. B.; Pert, A. Nicotinic binding in rat brain: autoradiographic comparison of [3H]acetylcholine, [3H]nicotine, and [125I]-alpha-bungarotoxin. J. Neurosci., 1985, 5(5), 1307-15.
41. Karlin, A. Emerging structure of the nicotinic acetylcholine receptors. Nat. Rev. Neurosci., 2002, 3(2), 102-14.
42. Albuquerque, E. X.; Pereira, E. F.; Alkondon, M.; Rogers, S. W. Mammalian nicotinic acetylcholine receptors: from structure to function. Physiol. Rev., 2009, 89(1), 73-120.
43. Unwin, N. Nicotinic acetylcholine receptor at 9 A resolution. J. Mol. Biol., 1993, 229(4), 1101-24.
44. Miyazawa, A.; Fujiyoshi, Y.; Stowell, M.; Unwin, N. Nicotinic acetylcholine receptor at 4. 6 A resolution: transverse tunnels in the cannel wall. J. Mol. Biol., 1999, 288(4), 765-86.
45. Miyazawa, A.; Fujiyoshi, Y.; Unwin, N. Structure and gating mechanism of the acetylcholine receptor pore. Nature, 2003, 423(6943), 949-55.
46. Unwin, N. Nicotinic acetylcholine receptor and the structural basis of neuromuscular transmission: insights from Torpedo postsynaptic membranes. Q. Rev. Biophys., 2013, 46(4), 283-322.
47. Auerbach, A.; Akk, G. Desensitization of mouse nicotinic acetylcholine receptor channels. A two-gate mechanism. J. Gen. Physiol., 1998, 112(2), 181-97.
48. Chen, J.; Zhang, Y.; Akk, G.; Sine, S.; Auerbach, A. Activation kinetics of recombinant mouse nicotinic acetylcholine receptors: mutations of alpha-subunit tyrosine 190 affect both binding and gating. Biophys. J., 1995, 69(3), 849-59.
49. Auerbach, A.; Sigurdson, W.; Chen, J.; Akk, G. Voltage dependence of mouse acetylcholine receptor gating: different charge movements in di-, mono-and unliganded receptors. J. Physiol., 1996, 494 (Pt 1), 155-70.
50. McIntosh, J. M.; Absalom, N.; Chebib, M.; Elgoyhen, A. B.; Vincler, M. Alpha9 nicotinic acetylcholine receptors and the treatment of pain. Biochem. Pharmacol., 2009, 78(7), 693-702.
51. Fabian-Fine R.; Skehel, P.; Errington, M. L.; Davies, H. A.; Sher, E.; Stewart, M. G.; Fine A. Ultrastructural distribution of the alpha7 nicotinic acetylcholine receptor subunit in rat hippocampus. J. Neurosci., 2001, 21(20), 7993-8003.
52. Caterina, M. J.; Schumacher, M. A.; Tominaga, M.; Rosen, T. A.; Levine, J. D.; Julius, D. The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature, 1997, 389(6653), 816-24.
53. Tominaga, M.; Caterina, M. J.; Malmberg, A. B.; Rosen, T. A.; Gilbert, H.; Skinner, K.; Raumann, B. E.; Basbaum, A. I.; Julius, D. The cloned capsaicin receptor integrates multiple painproducing stimuli. Neuron, 1998, 21(3), 531-43.
54. Salazar, H.; Llorente, I.; Jara-Oseguera, A.; Garcia-Villegas, R.; Munari, M.; Gordon, S. E.; Islas, L. D.; Rosenbaum, T. A single Nterminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. Nat. Neurosci., 2008, 11(3), 255-61.
55. Khomula, E. V.; Viatchenko-Karpinski, V. Y.; Borisyuk, A. L.; Duzhyy, D. E.; Belan, P. V.; Voitenko, N. V. Specific functioning of Cav3. 2 T-type calcium and TRPV1 channels under different types of STZ-diabetic neuropathy. Biochim. Biophys. Acta, 2013, 1832(5), 636-49.
56. Pabbidi, R. M.; Yu, S. Q.; Peng, S.; Khardori, R.; Pauza, M. E.; Premkumar, L. S. Influence of TRPV1 on diabetes-induced alterations in thermal pain sensitivity. Mol. Pain, 2008, 4, 9.
57. Engler, A.; Aeschlimann, A.; Simmen, B. R.; Michel, B. A.; Gay, R. E.; Gay, S.; Sprott, H. Expression of transient receptor potential vanilloid 1 (TRPV1) in synovial fibroblasts from patients with osteoarthritis and rheumatoid arthritis. Biochem. Biophys. Res. Commun., 2007, 359(4), 884-8.
58. Kelly, S.; Chapman, R. J.; Woodhams, S.; Sagar, D. R.; Turner, J.; Burston, J. J.; Bullock C., Paton K., Huang J., Wong A., McWilliams D. F., Okine B. N., Barrett D. A., Hathway G. J., Walsh D. A., Chapman V. Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain. Ann. Rheum. Dis., 2013.
59. Pan, H. L.; Zhang, Y. Q.; Zhao, Z. Q. Involvement of lysophosphatidic acid in bone cancer pain by potentiation of TRPV1 via PKCepsilon pathway in dorsal root ganglion neurons. Mol. Pain, 2010, 6, 85.
60. Cui, Y. Y.; Xu, H.; Wu, H. H.; Qi, J.; Shi, J.; Li, Y. Q. Spatiotemporal expression and functional involvement of transient receptor potential vanilloid 1 in diabetic mechanical allodynia in rats. PLoS One, 2014, 9(7), e102052.
61. Bowman, C. L.; Gottlieb, P. A.; Suchyna, T. M.; Murphy, Y. K.; Sachs, F. Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacology. Toxicon, 2007, 49(2), 249-70.
62. Gottlieb, P. A.; Suchyna, T. M.; Sachs, F. Properties and Mechanism of the Mechanosensitive Ion Channel Inhibitor GsMTx4, a Therapeutic Peptide Derived from Tarantula Venom. Curr. Top. Membr., 2007, 59, 81-109.
63. Hamill, O. P.; Martinac, B. Molecular basis of mechanotransduction in living cells. Physiol. Rev., 2001, 81(2), 685-740.
64. Kimelberg, H. K. O.; Anderson, E.; Kettenmann, H. Swellinginduced changes in electrophysiological properties of cultured astrocytes and oligodendrocytes. II. Whole-cell currents. Brain Res., 1990, 529(1-2), 262-8.
65. Kimelberg, H. K.; Goderie, S. K.; Higman, S.; Pang, S.; Waniewski, R. A. Swelling-induced release of glutamate, aspartate, and taurine from astrocyte cultures. J. Neurosci., 1990, 10(5), 1583-91.
66. Kimelberg, H. K., Kettenmann, H. Swelling-induced changes in electrophysiological properties of cultured astrocytes and oligodendrocytes. I. Effects on membrane potentials, input impedance and cell-cell coupling. Brain Res., 1990, 529(1-2), 255-61.
67. Martinac, B. Mechanosensitive ion channels: molecules of mechanotransduction. J. Cell Sci., 2004, 117(Pt 12), 2449-60.
68. Delmas, P.; Coste, B. Mechano-gated ion channels in sensory systems. Cell, 2013, 155(2), 278-84.
69. Kamajaya, A.; Kaiser, J. T.; Lee, J.; Reid, M.; Rees, D. C. The Structure of a Conserved Piezo Channel Domain Reveals a Topologically Distinct beta Sandwich Fold. Structure, 2014, 22(10), 1520-7.
70. Mintz, I. M.; Venema, V. J.; Swiderek, K. M.; Lee, T. D.; Bean, B. P.; Adams, M. E. P-type calcium channels blocked by the spider toxin omega-Aga-IVA. Nature, 199, 355(6363), 827-9.
71. Catterall, W. A. Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes. Annu. Rev. Pharmacol. Toxicol., 1980, 20, 15-43.
72. Miller, C. The charybdotoxin family of K+ channel-blocking peptides. Neuron, 1995, 15(1), 5-10.
73. Dutertre, S.; Lewis, R. J. Use of venom peptides to probe ion channel structure and function. J. Biol. Chem., 2010, 285(18), 13315-20.
74. Olivera, B. M.; Gray, W. R.; Zeikus, R.; McIntosh, J. M.; Varga, J.; Rivier, J.; de Santos, V.; Cruz, L. J. Peptide neurotoxins from fishhunting cone snails. Science (New York, N. Y.), 1985, 230(4732), 1338-43.
75. Possani, L. D.; Becerril, B.; Delepierre, M.; Tytgat, J. Scorpion toxins specific for Na+-channels. Eur. J. Biochem., 1999, 264(2), 287-300.
76. Bergeron, Z. L.; Bingham, J. P. Scorpion toxins specific for potassium (K+) channels: a historical overview of peptide bioengineering. Toxins (Basel), 2012, 4(11), 1082-119.
77. Gao, Y. D.; Garcia, M. L. Interaction of agitoxin2, charybdotoxin, and iberiotoxin with potassium channels: selectivity between voltage-gated and Maxi-K channels. Proteins, 2003, 52(2), 146-54.
78. Visan, V.; Fajloun, Z.; Sabatier, J. M.; Grissmer, S. Mapping of maurotoxin binding sites on hKv1. 2, hKv1. 3, and hIKCa1 channels. Mol. Pharmacol., 2004, 66(5), 1103-12.
79. Fajloun, Z.; Ferrat, G.; Carlier, E.; M'Barek, S.; Regaya, I.; Fathallah, M.; Rochat, H.; Darbon, H.; de Waard, M.; Sabatier, J. M. Synthesis, 3-D structure, and pharmacology of a reticulated chimeric peptide derived from maurotoxin and Tsk scorpion toxins. Biochem. Biophys. Res. Commun., 2002, 291(3), 640-8.
80. Adams, M. E.; Bindokas, V. P.; Hasegawa, L.; Venema, V. J. Omega-agatoxins: novel calcium channel antagonists of two subtypes from funnel web spider (Agelenopsis aperta) venom. J. boil. Chem., 1990, 265(2), 861-7.
81. Kitaguchi, T.; Swartz, K. J. An inhibitor of TRPV1 channels isolated from funnel Web spider venom. Biochemistry, 2005, 44(47), 15544-9.
82. Adams, M. E. Agatoxins: ion channel specific toxins from the American funnel web spider, Agelenopsis aperta. TOXICON: J. I. S. T. 2004, 43(5), 509-25.
83. King, G. F. Modulation of insect Ca(v) channels by peptidic spider toxins. Toxicon: J. I. S. T., 2007, 49(4), 513-30.
84. Pringos, E.; Vignes, M.; Martinez, J.; Rolland, V. Peptide neurotoxins that affect voltage-gated calcium channels: a close-up on ω-agatoxins. Toxins, 2011, 3(1), 17-42.
85. Reily, M. D.; Holub, K. E.; Gray, W. R.; Norris, T. M.; Adams, M. E. Structure-activity relationships for P-type calcium channelselective omega-agatoxins. Nat. Struct. Biol., 1994, 1(12), 853-6.
86. Kuwada, M.; Teramoto, T.; Kumagaye, K. Y.; Nakajima, K.; Watanabe, T.; Kawai, T.; Kawakami, Y.; Niidome, T.; Sawada, K.; Nishizawa, Y. Omega-agatoxin-TK containing D-serine at position 46, but not synthetic omega-[L-Ser46]agatoxin-TK, exerts blockade of P-type calcium channels in cerebellar Purkinje neurons. Mol. Pharmacol., 1994, 46(4), 587-93.
87. Lee, H. C.; Wang, J. M.; Swartz, K. J. Interaction between extracellular Hanatoxin and the resting conformation of the voltage-sensor paddle in Kv channels. Neuron, 2003, 40(3), 527-36.
88. Swartz, K. J. Tarantula toxins interacting with voltage sensors in potassium channels. Toxicon: I. T. S., 2007, 49(2), 213-30.
89. Suchyna, T. M.; Johnson, J. H.; Hamer, K.; Leykam, J. F.; Gage, D. A.; Clemo, H. F.; Baumgarten, C. M.; Sachs, F. Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channels. J. Gen. Physiol., 2000, 115(5), 583-98.
90. Siemens, J.; Zhou, S.; Piskorowski, R.; Nikai, T.; Lumpkin, E. A.; Basbaum, A. I.; King, D.; Julius, D. Spider toxins activate the capsaicin receptor to produce inflammatory pain. Nature, 2006, 444(7116), 208-12.
91. Bohlen, C. J.; Priel, A.; Zhou, S.; King, D.; Siemens, J.; Julius D. A bivalent tarantula toxin activates the capsaicin receptor, TRPV1, by targeting the outer pore domain. Cell, 2010, 141(5), 834-45.
92. Olivera, B. M. Conus peptides: biodiversity-based discovery and exogenomics. J. biol. chem., 2006, 281(42), 31173-7.
93. Olivera, B. M.; Teichert, R. W. Diversity of the neurotoxic Conus peptides: a model for concerted pharmacological discovery. Mol. Interventions, 2007, 7(5), 251-60.
94. Terlau, H.; Olivera, B. M. Conus venoms: a rich source of novel ion channel-targeted peptides. Physiol. Rev., 2004, 84(1), 41-68.
95. Bosmans, F.; Tytgat, J. Sea anemone venom as a source of insecticidal peptides acting on voltage-gated Na+ channels. Toxicon, 2007, 49(4), 550-60.
96. Honma, T.; Shiomi, K. Peptide toxins in sea anemones: structural and functional aspects. Marine biotechnology (New York, N. Y.), 2006, 8, 1-10.
97. Barhanin, J.; Hugues, M.; Schweitz, H.; Vincent, J. P.; Lazdunski, M. Structure-function relationships of sea anemone toxin II from Anemonia sulcata. J. Biol. Chem., 1981, 256(11), 5764-9.
98. Benzinger, G. R.; Kyle, J. W.; Blumenthal, K. M.; Hanck, D. A. A specific interaction between the cardiac sodium channel and site-3 toxin anthopleurin B. J. Biol. Chem., 1998, 273(1), 80-4.
99. Khera, P. K.; Blumenthal, K. M. Importance of highly conserved anionic residues and electrostatic interactions in the activity and structure of the cardiotonic polypeptide anthopleurin B. Biochemistry, 1996, 35(11), 3503-7.
100. Gooley, P. R.; Blunt, J. W.; Norton, R. S. Conformational heterogeneity in polypeptide cardiac stimulants from sea anemones. FEBS Lett., 1984, 174(1), 15-9.
101. Seibert, A. L.; Liu, J.; Hanck, D. A.; Blumenthal, K. M. Role of Asn-16 and Ser-19 in anthopleurin B binding. Implications for the electrostatic nature of Na(V) site 3. Biochemistry, 2004, 43(22), 7082-9.
102. Castaneda, O.; Sotolongo, V.; Amor, A. M.; Stocklin, R.; Anderson, A. J.; Harvey, A. L.; Engstrom, A.; Wernstedt, C.; Karlsson, E. Characterization of a potassium channel toxin from the Caribbean Sea anemone Stichodactyla helianthus. Toxicon, 1995, 33(5), 603-13.
103. Racape, J.; Lecoq, A.; Romi-Lebrun, R.; Liu, J.; Kohler, M.; Garcia, M. L.; Menez, A; Gasparini, S. Characterization of a novel radiolabeled peptide selective for a subpopulation of voltage-gated potassium channels in mammalian brain. J. Biol. Chem., 2002, 277(6), 3886-93.
104. Gilquin, B.; Braud, S.; Eriksson, M. A.; Roux, B.; Bailey, T. D.; Priest, B. T.; Garcia, M. L.; Menez, A.; Gasparini, S. A variable residue in the pore of Kv1 channels is critical for the high affinity of blockers from sea anemones and scorpions. J. Biol. Chem., 2005, 280(29), 27093-102.
105. Dufton, M. J.; Hider, R. C. Structure and pharmacology of elapid cytotoxins. Pharmacol. Ther., 1988, 36(1), 1-40.
106. Endo T., Tamiya N. Current view on the structure-function relationship of postsynaptic neurotoxins from snake venoms. Pharmacol. Ther., 1987, 34(3), 403-51.
107. Chang, C. C.; Lee, C. Y. Isolation of Neurotoxins from the Venom of Bungarus Multicinctus and Their Modes of Neuromuscular Blocking Action. Arch. Int. Pharmacodyn. Ther., 1963, 144, 241-57.
108. Barber, C. M.; Isbister, G. K.; Hodgson, W. C. Alpha neurotoxins. Toxicon, 2013, 66, 47-58.
109. Love, R. A.; Stroud, R. M. The crystal structure of alphabungarotoxin at 2. 5 A resolution: relation to solution structure and binding to acetylcholine receptor. Protein Eng., 1986, 1(1), 37-46.
110. Wang, F. C.; Parcej, D. N.; Dolly, J. O. alpha subunit compositions of Kv1. 1-containing K+ channel subtypes fractionated from rat brain using dendrotoxins. Eur. J. Biochem., 1999, 263(1), 230-7.
111. Rowan, E. G.; Harvey, A. L. Snake toxins from mamba venoms: unique tools for the physiologist. Acta. Chim. Slov., 2011, 58(4), 689-92.
112. Tsuda, K.; Kawamura, M. The constituents of the ovaries of globefish. VIII. Studies on tetrodotoxin. Pharm Bull, 1953, 1(2), 112-3.
113. Matsumura, M.; Yamamoto, S. The effect of tetrodotoxin on the neuromuscular junction and peripheral nerve of the toad. Jpn. J. Pharmacol., 1954, 4(1), 62-8.
114. Fuhrman, F. A. Tetrodotoxin. It is a powerful poison that is found in two almost totally unrelated kinds of animal: puffer fish and newts. It has been serving as a tool in nerve physiology and may provide a model for new local anesthetics. Sci. Am., 1967, 217(2), 60-71.
115. Hwang, D. F.; Tai, K. P.; Chueh, C. H.; Lin, L. C.; Jeng, S. S. Tetrodotoxin and derivatives in several species of the gastropod Naticidae. Toxicon: J. I. T. S, 1991, 29(8), 1019-24.
116. Mebs, D.; Schmidt, K. Occurrence of tetrodotoxin in the frog Atelopus oxyrhynchus. Toxicon: J. I. T. S, 1989, 27(7), 819-22.
117. Yasumoto, T.; Nagai, H.; Yasumura, D.; Michishita, T.; Endo, A.; Yotsu, M.; Kotaki, Y. Interspecies distribution and possible origin of tetrodotoxin. Ann. N. Y. Acad. Sci., 1986, 479, 44-51.
118. Jost, M. C.; Hillis, D. M.; Lu, Y.; Kyle, J. W.; Fozzard, H. A.; Zakon, H. H. Toxin-resistant sodium channels: parallel adaptive evolution across a complete gene family. Mol. Biol. Evol., 2008, 25(6), 1016-24.
119. Kaneko, Y.; Matsumoto, G.; Hanyu, Y. TTX resistivity of Na+ channel in newt retinal neuron. Biochem. Biophys. Res. Commun., 1997, 240(3), 651-6.
120. Maruta, S.; Yamaoka, K.; Yotsu-Yamashita M. Two critical residues in p-loop regions of puffer fish Na+ channels on TTX sensitivity. Toxicon: J. I. T. S, 2008, 51(3), 381-7.
121. Venkatesh, B.; Lu, S. Q.; Dandona, N.; See, S. L.; Brenner, S.; Soong, T. W. Genetic basis of tetrodotoxin resistance in pufferfishes. Current biology: CB, 2005, 15(22), 2069-72.
122. Geffeney, S. L.; Fujimoto, E.; Brodie, E. D.; Ruben, P. C. Evolutionary diversification of TTX-resistant sodium channels in a predator-prey interaction. Nature, 2005, 434(7034), 759-63.
123. Black, J. A.; Frezel, N.; Dib-Hajj, S. D.; Waxman, S. G. Expression of Nav1. 7 in DRG neurons extends from peripheral terminals in the skin to central preterminal branches and terminals in the dorsal horn. Mol Pain, 2012, 8, 82.
124. Pinto, V.; Derkach, V. A.; Safronov, B. V. Role of TTX-sensitive and TTX-resistant sodium channels in Adelta-and C-fiber conduction and synaptic transmission. J. Neurophysiol., 2008, 99(2), 617-28.
125. Ritter, A. M.; Mendell, L. M. Somal membrane properties of physiologically identified sensory neurons in the rat: effects of nerve growth factor. J. Neurophysiol., 1992, 68(6), 2033-41.
126. Villiere, V.; McLachlan, E. M. Electrophysiological properties of neurons in intact rat dorsal root ganglia classified by conduction velocity and action potential duration. J. Neurophysiol., 1996, 76(3), 1924-41.
127. Yoshida, S.; Matsuda, Y. Studies on sensory neurons of the mouse with intracellular-recording and horseradish peroxidase-injection techniques. J. Neurophysiol., 1979, 42(4), 1134-45.
128. Clark, R. F.; Williams, S. R.; Nordt, S. P.; Manoguerra, A. S. A review of selected seafood poisonings. Underse. Hypered, 1999, 26(3), 175-84.
129. Thottumkara, A. P.; Parsons, W. H.; Du, Bois J. Saxitoxin. Angew. Chem. Int. Ed. Engl., 2014, 53(23), 5760-84.
130. Benoit, E.; Juzans, P.; Legrand, A. M.; Molgo, J. Nodal swelling produced by ciguatoxin-induced selective activation of sodium channels in myelinated nerve fibers. Neuroscience, 1996, 71(4), 1121-31.
131. Durand-Clement, M. A study of toxin production by Gambierdiscus toxicus in culture. Toxicon, 1986, 24(11-12), 1153-7.
132. Lombet, A.; Bidard, J. N.; Lazdunski, M. Ciguatoxin and brevetoxins share a common receptor site on the neuronal voltagedependent Na+ channel. FEBS Lett., 1987, 219(2), 355-9.
133. Catterall, W. A.; Gainer, M. Interaction of brevetoxin A with a new receptor site on the sodium channel. Toxicon, 1985, 23(3), 497-504.
134. Gawley, R. E.; Rein, K. S.; Kinoshita, M.; Baden, D. G. Binding of brevetoxins and ciguatoxin to the voltage-sensitive sodium channel and conformational analysis of brevetoxin B. Toxicon, 1992, 30(7), 780-5.
135. Appendino, G.; Szallasi, A. Euphorbium: modern research on its active principle, resiniferatoxin, revives an ancient medicine. Life Sci., 1997, 60(10), 681-96.
136. Chou, M. Z.; Mtui, T.; Gao, Y. D.; Kohler, M.; Middleton, R. E. Resiniferatoxin binds to the capsaicin receptor (TRPV1) near the extracellular side of the S4 transmembrane domain. Biochemistry, 2004, 43(9), 2501-11.
137. Rodriguez de la Vega R. C.; Possani, L. D. Current views on scorpion toxins specific for K+-channels. Toxicon, 2004, 43(8), 865-75.
138. Hermann, A.; Erxleben, C. Charybdotoxin selectively blocks small Ca-activated K channels in Aplysia neurons. J. Gen. Physiol., 1987, 90(1), 27-47.
139. Miller, C.; Moczydlowski, E.; Latorre, R.; Phillips, M. Charybdotoxin, a protein inhibitor of single Ca2+-activated K+ channels from mammalian skeletal muscle. Nature, 1985, 313(6000), 316-8.
140. Goldstein, S. A.; Miller, C. Mechanism of charybdotoxin block of a voltage-gated K+ channel. Biophys. J., 1993, 65(4), 1613-9.
141. MacKinnon, R.; Heginbotham, L.; Abramson, T. Mapping the receptor site for charybdotoxin, a pore-blocking potassium channel inhibitor. Neuron, 1990, 5(6), 767-71.
142. MacKinnon, R.; Miller, C. Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor. Science, 1989, 245(4924), 1382-5.
143. Anderson, C. S.; MacKinnon, R.; Smith, C.; Miller, C. Charybdotoxin block of single Ca2+-activated K+ channels. Effects of channel gating, voltage, and ionic strength. J. Gen. Physiol., 1988, 91(3), 317-33.
144. MacKinnon, R.; Miller, C. Mechanism of charybdotoxin block of the high-conductance, Ca2+-activated K+ channel. J. Gen. Physiol., 1988, 91(3), 335-49.
145. Park, C. S.; Miller, C. Interaction of charybdotoxin with permeant ions inside the pore of a K+ channel. Neuron, 1992, 9(2), 307-13.
146. Banerjee, A.; Lee, A.; Campbell, E.; Mackinnon, R. Structure of a pore-blocking toxin in complex with a eukaryotic voltagedependent K(+) channel. Elife, 2013, 2, e00594.
147. Escobar, L.; Root, M. J.; MacKinnon, R. Influence of protein surface charge on the bimolecular kinetics of a potassium channel peptide inhibitor. Biochemistry, 1993, 32(27), 6982-7.
148. Goldstein, S. A.; Pheasant, D. J.; Miller, C. The charybdotoxin receptor of a Shaker K+ channel: peptide and channel residues mediating molecular recognition. Neuron, 1994, 12(6), 1377-88.
149. Naranjo, D.; Miller, C. A strongly interacting pair of residues on the contact surface of charybdotoxin and a Shaker K+ channel. Neuron, 1996, 16(1), 123-30.
150. Heginbotham, L.; Lu, Z.; Abramson, T.; MacKinnon, R. Mutations in the K+ channel signature sequence. Biophys. J., 1994, 66(4), 1061-7.
151. Lippiat, J. D.; Standen, N. B.; Davies, N. W. A residue in the intracellular vestibule of the pore is critical for gating and permeation in Ca2+-activated K+ (BKCa) channels. J. Physiol., 2000, 529 Pt 1, 131-8.
152. Ranganathan, R.; Lewis, J. H.; MacKinnon, R. Spatial localization of the K+ channel selectivity filter by mutant cycle-based structure analysis. Neuron, 1996, 16(1), 131-9.
153. Hidalgo, P.; MacKinnon, R. Revealing the architecture of a K+ channel pore through mutant cycles with a peptide inhibitor. Science, 1995, 268(5208), 307-10.
154. Lange, A.; Giller, K.; Hornig, S.; Martin-Eauclaire M. F.; Pongs, O.; Becker, S.; Baldus, M. Toxin-induced conformational changes in a potassium channel revealed by solid-state NMR. Nature, 2006, 440(7086), 959-62.
155. MacKinnon, R.; Reinhart, P. H.; White, M. M. Charybdotoxin block of Shaker K+ channels suggests that different types of K+ channels share common structural features. Neuron, 1988, 1(10), 997-1001.
156. MacKinnon, R. Determination of the subunit stoichiometry of a voltage-activated potassium channel. Nature, 1991, 350(6315), 232-5.
157. Ahlijanian, M. K.; Striessnig, J.; Catterall, W. A. Phosphorylation of an alpha 1-like subunit of an omega-conotoxin-sensitive brain calcium channel by cAMP-dependent protein kinase and protein kinase. J. Biol. Chem., 1991, 266(30), 20192-7.
158. Krasilnikov, O. V.; Merzlyak, P. G.; Yuldasheva, L. N.; Rodrigues, C. G.; Bhakdi, S.; Valeva, A. Electrophysiological evidence for heptameric stoichiometry of ion channels formed by Staphylococcus aureus alpha-toxin in planar lipid bilayers. Mol. Microbiol., 2000, 37(6), 1372-8.
159. Cotton, J.; Crest, M.; Bouet, F.; Alessandri, N.; Gola, M.; Forest, E.; Karlsson, E.; Castaneda, O.; Harvey, A. L.; Vita C.; Menez, A. A potassium-channel toxin from the sea anemone Bunodosoma granulifera, an inhibitor for Kv1 channels. Revision of the amino acid sequence, disulfide-bridge assignment, chemical synthesis, and biological activity. Eur. J. Biochem., 1997, 244(1), 192-202.
160. Gilquin, B.; Racapé, J.; Wrisch, A.; Visan, V.; Lecoq, A.; Grissmer, S.; Ménez, A.; Gasparini, S. Structure of the BgK-Kv1. 1 complex based on distance restraints identified by double mutant cycles. Molecular basis for convergent evolution of Kv1 channel blockers. J. biol. chem., 2002, 277(40), 37406-13.
161. Savarin, P.; Guenneugues, M.; Gilquin, B.; Lamthanh, H.; Gasparini, S.; Zinn-Justin, S.; Ménez, A. Three-dimensional structure of kappa-conotoxin PVIIA, a novel potassium channel-blocking toxin from cone snails. Biochemistry, 1998, 37(16), 5407-16.
162. Naranjo, D. Inhibition of single Shaker K channels by kappaconotoxin-PVIIA. Biophys. J., 2002, 82(6), 3003-11.
163. Jacobsen, R. B.; Koch, E. D.; Lange-Malecki, B.; Stocker, M.; Verhey, J.; Van, Wagoner R. M.; Vyazovkina, A.; Olivera, B. M.; Terlau, H. Single amino acid substitutions in kappa-conotoxin PVIIA disrupt interaction with the shaker K+ channel. J. biol. chem., 2000, 275(32), 24639-44.
164. Leipold, E.; Hansel, A.; Olivera, B. M.; Terlau, H.; Heinemann, S. H. Molecular interaction of delta-conotoxins with voltage-gated sodium channels. FEBS Lett., 2005, 579(18), 3881-4.
165. Gurevitz, M. Mapping of scorpion toxin receptor sites at voltagegated sodium channels. Toxicon, 2012, 60(4), 502-11.
166. Cestele S., Catterall W. A. Molecular mechanisms of neurotoxin action on voltage-gated sodium channels. Biochimie, 2000, 82(9-10), 883-92.
167. Catterall, W. A.; Cestele, S.; Yarov-Yarovoy, V.; Yu, F. H.; Konoki, K.; Scheuer, T. Voltage-gated ion channels and gating modifier toxins. Toxicon, 2007, 49(2), 124-41.
168. Hui, K.; Lipkind, G.; Fozzard, H. A.; French, R. J. Electrostatic and steric contributions to block of the skeletal muscle sodium channel by mu-conotoxin. J. gen. physio., 2002, 119(1), 45-54.
169. Hill, J. M.; Alewood, P. F.; Craik, D. J. Three-dimensional solution structure of mu-conotoxin GIIIB, a specific blocker of skeletal muscle sodium channels. Biochemistry, 1996, 35(27), 8824-35.
170. Wakamatsu, K.; Kohda, D.; Hatanaka, H.; Lancelin, J. M.; Ishida, Y.; Oya, M.; Nakamura, H.; Inagaki, F.; Sato, K. Structure-activity relationships of mu-conotoxin GIIIA: structure determination of active and inactive sodium channel blocker peptide by NMR and simulated annealing calculations. Biochemistry, 1992, 31(50), 12577-84.
171. Goldin, A. L.; Barchi, R. L.; Caldwell, J. H.; Hofmann, F.; Howe, J. R.; Hunter, J. C.; Kallen, R. G.; Mandel, G.; Meisler, M. H.; Netter, Y. B.; Noda, M.; Tamkun, M. M.; Waxman, S. G.; Wood, J. N.; Catterall, W. A. Nomenclature of voltage-gated sodium channels. Neuron, 2000, 28(2), 365-8.
172. Moczydlowski, E.; Olivera, B. M.; Gray, W. R.; Strichartz, G. R. Discrimination of muscle and neuronal Na-channel subtypes by binding competition between [3H]saxitoxin and mu-conotoxins. Proc. N. Acad. Sci U S A, 1986, 83(14), 5321-5.
173. Ohizumi, Y.; Nakamura, H.; Kobayashi, J.; Catterall, W. A. Specific inhibition of [3H] saxitoxin binding to skeletal muscle sodium channels by geographutoxin II, a polypeptide channel blocker. J. boil. Chem., 1986, 261(14), 6149-52.
174. Yanagawa, Y.; Abe, T.; Satake, M. Blockade of [3H]lysinetetrodotoxin binding to sodium channel proteins by conotoxin GIII. Neurosci. Lett., 1986, 64(1), 7-12.
175. Chahine, M.; Chen, L. Q.; Fotouhi, N.; Walsky, R.; Fry, D.; Santarelli, V.; Horn, R.; Kallen, R. G. Characterizing the mu-conotoxin binding site on voltage-sensitive sodium channels with toxin analogs and channel mutations. Receptors & channels, 1995, 3(3), 161-74.
176. Chahine, M.; Sirois, J.; Marcotte, P.; Chen, L.; Kallen, R. G. Extrapore residues of the S5-S6 loop of domain 2 of the voltage-gated skeletal muscle sodium channel (rSkM1) contribute to the muconotoxin GIIIA binding site. Biophys. J., 1998, 75(1), 236-46.
177. Dudley, S. C.; Todt, H.; Lipkind, G.; Fozzard, H. A. A muconotoxin-insensitive Na+ channel mutant: possible localization of a binding site at the outer vestibule. Biophys. J., 1995, 69(5), 1657-65.
178. Stephan, M. M.; Potts, J. F.; Agnew, W. S. The microI skeletal muscle sodium channel: mutation E403Q eliminates sensitivity to tetrodotoxin but not to mu-conotoxins GIIIA and GIIIB. J. Membr. Biol., 1994, 137(1), 1-8.
179. Becker, S.; Prusak-Sochaczewski, E.; Zamponi, G.; Beck-Sickinger, A. G.; Gordon, R. D.; French, R. J. Action of derivatives of mu-conotoxin GIIIA on sodium channels. Single amino acid substitutions in the toxin separately affect association and dissociation rates. Biochemistry, 1992, 31(35), 8229-38.
180. Sato, K.; Ishida, Y.; Wakamatsu, K.; Kato, R.; Honda, H.; Ohizumi, Y.; Nakamura, H.; Ohya, M.; Lancelin, J. M.; Kohda, D. Active site of mu-conotoxin GIIIA, a peptide blocker of muscle sodium channels. J. boil. chem., 1991, 266(26), 16989-91.
181. French, R. J.; Prusak-Sochaczewski, E.; Zamponi, G. W.; Becker, S.; Kularatna, A. S.; Horn R. Interactions between a pore-blocking peptide and the voltage sensor of the sodium channel: an electrostatic approach to channel geometry. Neuron, 1996, 16(2), 407-13.
182. Li, R. A.; Sato, K.; Kodama, K.; Kohno, T.; Xue, T.; Tomaselli, G. F.; Marbán, E. Charge conversion enables quantification of the proximity between a normally-neutral mu-conotoxin (GIIIA) site and the Na+ channel pore. FEBS Lett., 2002, 511(1-3), 159-64.
183. Li, R. A.; Ennis, I. L.; French, R. J.; Dudley, S. C. Jr.; Tomaselli, G. F.; Marban, E. Clockwise domain arrangement of the sodium channel revealed by (mu)-conotoxin (GIIIA) docking orientation. J. Biol. Chem., 2001, 276(14), 11072-7.
184. Dudley, S. C.; Chang, N.; Hall, J.; Lipkind, G.; Fozzard, H. A.; French, R. J. mu-conotoxin GIIIA interactions with the voltagegated Na(+) channel predict a clockwise arrangement of the domains. J. gen. physio., 2000, 116(5), 679-90.
185. Bezanilla, F.; Armstrong, C. M. Gating currents of the sodium channels: three ways to block them. Science (New York, N. Y.), 1974, 183(4126), 753-4.
186. Hille, B. The receptor for tetrodotoxin and saxitoxin. A structural hypothesis. Biophys. J., 1975, 15(6), 615-9.
187. Kao C. Y., Nishiyama A. Actions of saxitoxin on peripheral neuromuscular systems. J. Physiol, 1965, 180(1), 50-66.
188. Keynes, R. D.; Rojas, E. Kinetics and steady-state properties of the charged system controlling sodium conductance in the squid giant axon. J. physio., 1974, 239(2), 393-434.
189. Gilchrist, J.; Olivera, B. M.; Bosmans, F. Animal toxins influence voltage-gated sodium channel function. Handb Exp Pharmacol. 2212014. p. 203-29.
190. Goto, T.; Kishi, Y.; Takahashi, S.; Hirata, T. The structure op tetrodotoxin. Tetrahedron Lett., 1963, 4(30), 2105-13.
191. Tsuda, K.; Tachikawa, R.; Sakai, K.; Tamura, C.; Amakasu, O.; Kawamura, M.; Ikuma, S. On the Structure of Tetrodotoxin. Chem. Pharm. Bull. (Tokyo), 1964, 12, 642-5.
192. Hille, B. Charges and potentials at the nerve surface. Divalent ions and pH. J. Gen. Physiol., 1968, 51(2), 221-36.
193. Hille, B. The permeability of the sodium channel to organic cations in myelinated nerve. J. Gen. Physiol., 1971, 58(6), 599-619.
194. Zhang, X.; Ren, W.; DeCaen, P.; Yan, C.; Tao, X.; Tang, L.; Wang, J.; Hasegawa, K.; Kumasaka, T.; He, J.; Wang, J.; Clapham, D. E.; Yan, N. Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel. Nature, 2012, 486(7401), 130-4.
195. Payandeh, J.; Scheuer, T.; Zheng, N.; Catterall, W. A. The crystal structure of a voltage-gated sodium channel. Nature, 2011, 475(7356), 353-8.
196. Payandeh, J.; Gamal, El-Din T. M.; Scheuer, T.; Zheng, N.; Catterall, W. A. Crystal structure of a voltage-gated sodium channel in two potentially inactivated states. Nature, 2012, 486(7401), 135-9.
197. Narahashi, T. Chemicals as tools in the study of excitable membranes. Physiol. Rev., 1974, 54(4), 813-89.
198. Narahashi, T.; Moore, J. W.; Scott, W. R. Tetrodotoxin Blockage of Sodium Conductance Increase in Lobster Giant Axons. J. Gen. Physiol., 1964, 47, 965-74.
199. Ritchie, J. M.; Rogart, R. B. The binding of saxitoxin and tetrodotoxin to excitable tissue. Rev. Physiol. Biochem. Pharmacol., 1977, 79, 1-50.
200. Henderson, R.; Ritchie, J. M.; Strichartz, G. R. Evidence that tetrodotoxin and saxitoxin act at a metal cation binding site in the sodium channels of nerve membrane. Proc. Natl. Acad. Sci. U S A, 1974, 71(10), 3936-40.
201. Lipkind, G. M.; Fozzard, H. A. A structural model of the tetrodotoxin and saxitoxin binding site of the Na+ channel. Biophys. J., 1994, 66(1), 1-13.
202. Tikhonov, D. B.; Zhorov, B. S. Architecture and pore block of eukaryotic voltage-gated sodium channels in view of NavAb bacterial sodium channel structure. Mol. Pharmacol., 2012, 82(1), 97-104.
203. Chen, R.; Chung, S. H. Binding modes of mu-conotoxin to the bacterial sodium channel (NavAb). Biophys. J., 2012, 102(3), 483-8.
204. Penzotti, J. L.; Fozzard, H. A.; Lipkind, G. M.; Dudley, S. C. Differences in saxitoxin and tetrodotoxin binding revealed by mutagenesis of the Na+ channel outer vestibule. Biophys. J., 1998, 75(6), 2647-57.
205. Terlau, H.; Heinemann, S. H.; Stühmer, W.; Pusch, M.; Conti, F.; Imoto, K.; Numa, S. Mapping the site of block by tetrodotoxin and saxitoxin of sodium channel II. FEBS Lett., 1991, 293(1-2), 93-6.
206. Chen, R.; Chung, S. H. Mechanism of tetrodotoxin block and resistance in sodium channels. Biochem. Biophys. Res. Commun., 2014, 446, 370-4.
207. Feldman, C. R.; Brodie, E. D. Jr.; Brodie, E. D.; 3rd, Pfrender M. E. Constraint shapes convergence in tetrodotoxin-resistant sodium channels of snakes. Proc. Natl. Acad. Sci. U S A, 2012, 109(12), 4556-61.
208. Payandeh, J.; Scheuer, T.; Zheng, N.; Catterall, W. A. The crystal structure of a voltage-gated sodium channel. Nature, 2011, 475(7356), 353-8.
209. Taraska, J. W., Puljung, M. C.; Olivier, N. B.; Flynn, G. E.; Zagotta, W. N. Mapping the structure and conformational movements of proteins with transition metal ion FRET. Nat. Methods, 2009, 6(7), 532-7.
210. Angelides, K. J.; Brown, G. B. Fluorescence resonance energy transfer on the voltage-dependent sodium channel. Spatial relationship and site coupling between the batrachotoxin and Leiurus quinquestriatus quinquestriatus alpha-scorpion toxin receptors. J. Biol. Chem., 1984, 259(10), 6117-26.
211. Du, Y.; Garden, D.; Khambay, B.; Zhorov, B. S.; Dong, K. Batrachotoxin, pyrethroids, and BTG 502 share overlapping binding sites on insect sodium channels. Mol. Pharmacol., 2011, 80(3), 426-33.
212. Wang, S. Y.; Mitchell, J.; Tikhonov, D. B.; Zhorov, B. S.; Wang, G. K. How batrachotoxin modifies the sodium channel permeation pathway: computer modeling and site-directed mutagenesis. Mol. Pharmacol., 2006, 69(3), 788-95.
213. Campos, F. V.; Chanda, B.; Beirao, P. S.; Bezanilla, F. Alphascorpion toxin impairs a conformational change that leads to fast inactivation of muscle sodium channels. J. Gen. Physiol., 2008, 132(2), 251-63.
214. Angelides, K. J.; Nutter, T. J. Mapping the molecular structure of the voltage-dependent sodium channel. Distances between the tetrodotoxin and Leiurus quinquestriatus quinquestriatus scorpion toxin receptors. J. Biol. Chem., 1983, 258(19), 11958-67.
215. Stocker, J. W.; Nadasdi, L.; Aldrich, R. W.; Tsien, R. W. Preferential interaction of omega-conotoxins with inactivated N-type Ca2+ channels. J. Neurosci., 1997, 17(9), 3002-13.
216. Altier, C.; Dale, C. S.; Kisilevsky, A. E.; Chapman, K.; Castiglioni, A. J.; Matthews, E. A.; Evans, R. M.; Dickenson, A. H., Lipscombe D., Vergnolle N., Zamponi G. W. Differential role of N-type calcium channel splice isoforms in pain. J. Neurosci., 2007, 27(24), 6363-73.
217. Raingo, J.; Castiglioni, A. J.; Lipscombe, D. Alternative splicing controls G protein-dependent inhibition of N-type calcium channels in nociceptors. Nat. Neurosci., 2007, 10(3), 285-92.
218. Schmidtko, A.; Lötsch, J.; Freynhagen, R.; Geisslinger, G. Ziconotide for treatment of severe chronic pain. Lancet, 2010, 375(9725), 1569-77.
219. Calcagnotto, M. E.; Baraban, S. C. An examination of calcium current function on heterotopic neurons in hippocampal slices from rats exposed to methylazoxymethanol. Epilepsia, 2003, 44(3), 315-21.
220. Imaizumi T., Kocsis J. D., Waxman S. G. The role of voltage-gated Ca2+ channels in anoxic injury of spinal cord white matter. Brain Res., 1999, 817(1-2), 84-92.
221. McCleskey, E. W.; Fox, A. P.; Feldman, D. H.; Cruz, L. J.; Olivera, B. M.; Tsien, R. W.; Yoshikami, D. Omega-conotoxin: direct and persistent blockade of specific types of calcium channels in neurons but not muscle. Proc. Nat. Acad. Sci. U. S. A., 1987, 84(12), 4327-31.
222. Wennemuth, G.; Westenbroek, R. E.; Xu, T.; Hille, B.; Babcock, D. F. CaV2. 2 and CaV2. 3 (N-and R-type) Ca2+ channels in depolarization-evoked entry of Ca2+ into mouse sperm. J. biol. chem., 2000, 275(28), 21210-7.
223. Olivera, B. M.; Miljanich, G. P.; Ramachandran, J.; Adams, M. E. Calcium channel diversity and neurotransmitter release: the omegaconotoxins and omega-agatoxins. Annu. Rev. Biochem., 1994, 63, 823-67.
224. Miljanich, G. P. Ziconotide: neuronal calcium channel blocker for treating severe chronic pain. Curr. Med. Chem., 2004, 11(23), 3029-40.
225. Smith, H. S.; Deer, T. R. Safety and efficacy of intrathecal ziconotide in the management of severe chronic pain. Ther. Clin. Risk. Manag., 2009, 5(3), 521-34.
226. Adams D. J.; Berecki, G. Mechanisms of conotoxin inhibition of N-type (Ca(v)2. 2) calcium channels. Biochim. Biophys. Acta, 2013, 1828(7), 1619-28.
227. Scanlon, M. J.; Naranjo, D.; Thomas, L.; Alewood, P. F.; Lewis, R. J.; Craik, D. J. Solution structure and proposed binding mechanism of a novel potassium channel toxin kappa-conotoxin PVIIA. Structure (London, England: 1993), 1997, 5(12), 1585-97.
228. Schroeder, C. I.; Lewis, R. J. a. A. D. J. Block of Voltage-Gated Calcium Channels by Peptide Toxins. In: Zamponi GW, editor. Georgetown, Texas: Bookshelf, Madame Curie Bioscience Database-NCBI; 2000. p. 294-308.
229. Nielsen, K. J.; Schroeder, T.; Lewis, R. Structure-activity relationships of omega-conotoxins at N-type voltage-sensitive calcium channels. J. Mol. Recognit, 2000, 13(2), 55-70.
230. Schroeder, C. I.; Nielsen, K. J.; Adams, D. A.; Loughnan, M.; Thomas, L.; Alewood, P. F.; Lewis, R. J.; Craik, D. J. Effects of Lys2 to Ala2 substitutions on the structure and potency of ω-conotoxins MVIIA and CVID. Biopolymers, 2012, 98(4), 345-56.
231. Chen, R.; Chung, S.-H. Complex structures between the N-type calcium channel (CaV2. 2) and ω-conotoxin GVIA predicted via molecular dynamics. Biochemistry, 2013, 52(21), 3765-72.
232. Kim, J. I.; Takahashi, M.; Martin-Moutot, N.; Seagar, M. J.; Ohtake, A.; Sato, K. Tyr13 is essential for the binding of omegaconotoxin MVIIC to the P/Q-type calcium channel. Biochem. Biophys. Res. Commun., 1995, 214(2), 305-9.
233. Kim, J. I.; Takahashi, M.; Ogura, A.; Kohno, T.; Kudo, Y.; Sato, K. Hydroxyl group of Tyr13 is essential for the activity of omegaconotoxin GVIA, a peptide toxin for N-type calcium channel. J. biol. chem., 1994, 269(39), 23876-8.
234. Sato, K.; Park, N. G.; Kohno, T.; Maeda, T.; Kim, J. I.; Kato, R.; Takahashi, M. Role of basic residues for the binding of omegaconotoxin GVIA to N-type calcium channels. Biochem. Biophys. Res. Commun., 1993, 194(3), 1292-6.
235. Ellinor, P. T.; Zhang, J. F.; Horne, W. A.; Tsien, R. W. Structural determinants of the blockade of N-type calcium channels by a peptide neurotoxin. Nature, 1994, 372(6503), 272-5.
236. McDonough, S. I.; Boland, L. M.; Mintz, I. M.; Bean, B. P. Interactions among toxins that inhibit N-type and P-type calcium channels. J. gen. physio., 2002, 119(4), 313-28.
237. Beress, L.; Beress, R. Purification of three polypeptides with neuro-and cardiotoxic activity from the sea anemone Anemonia sulcata. Toxicon, 1975, 13(5), 359-67.
238. Driscoll, P. C.; Clore, G. M.; Beress, L.; Gronenborn, A. M. A proton nuclear magnetic resonance study of the antihypertensive and antiviral protein BDS-I from the sea anemone Anemonia sulcata: sequential and stereospecific resonance assignment and secondary structure. Biochemistry, 1989, 28(5), 2178-87.
239. Yeung, S. Y.; Thompson, D.; Wang, Z.; Fedida, D.; Robertson, B. Modulation of Kv3 subfamily potassium currents by the sea anemone toxin BDS: significance for CNS and biophysical studies. J. Neurosci., 2005, 25(38), 8735-45.
240. Diochot, S.; Schweitz, H.; Beress, L.; Lazdunski, M. Sea anemone peptides with a specific blocking activity against the fast inactivating potassium channel Kv3. 4. J. Biol. Chem., 1998, 273(12), 6744-9.
241. Llewellyn, L. E.; Norton, R. S. Binding of the sea anemone polypeptide BDS II to the voltage-gated sodium channel. Biochem Int, 1991, 24(5), 937-46.
242. Liu, P.; Jo, S.; Bean, B. P. Modulation of neuronal sodium channels by the sea anemone peptide BDS-I. J. Neurophysiol., 2012, 107(11), 3155-67.
243. Swartz, K. J.; MacKinnon, R. An inhibitor of the Kv2. 1 potassium channel isolated from the venom of a Chilean tarantula. Neuron, 1995, 15(4), 941-9.
244. Swartz, K. J.; MacKinnon, R. Hanatoxin modifies the gating of a voltage-dependent K+ channel through multiple binding sites. Neuron, 1997, 18(4), 665-73.
245. Swartz, K. J.; MacKinnon, R. Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels. Neuron, 1997, 18, 675-82.
246. Phillips, L. R.; Milescu, M.; Li-Smerin, Y.; Mindell, J. A., Kim J. I., Swartz K. J. Voltage-sensor activation with a tarantula toxin as cargo. Nature, 2005, 436(7052), 857-60.
247. Li-Smerin, Y.; Swartz, K. J. Localization and molecular determinants of the Hanatoxin receptors on the voltage-sensing domains of a K(+) channel. J. gen. physio., 2000, 115(6), 673-84.
248. Lee, S. Y.; MacKinnon, R. A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom. Nature, 2004, 430(6996), 232-5.
249. Jung, H. J.; Lee, J. Y.; Kim, S. H.; Eu, Y. J.; Shin, S. Y.; Milescu, M.; Swartz, K. J.; Kim, J. I. Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel. Biochemistry, 2005, 44(16), 6015-23.
250. Smith, L. A.; Reid, P. F.; Wang, F. C.; Parcej, D. N.; Schmidt, J. J.; Olson, M. A.; Dolly, J. O. Site-directed mutagenesis of dendrotoxin K reveals amino acids critical for its interaction with neuronal K+ channels. Biochemistry, 1997, 36(25), 7690-6.
251. Imredy, J. P.; MacKinnon, R. Energetic and structural interactions between delta-dendrotoxin and a voltage-gated potassium channel. J. Mol. Biol., 2000, 296(5), 1283-94.
252. Imredy, J. P.; MacKinnon R. Energetic and structural interactions between delta-dendrotoxin and a voltage-gated potassium channel. J. mol. boil., 2000, 296(5), 1283-94.
253. Gasparini, S.; Danse, J. M.; Lecoq, A.; Pinkasfeld, S.; Zinn-Justin, S.; Young, L. C.; de Medeiros, C. C.; Rowan, E. G.; Harvey, a. L.; Ménez, A. Delineation of the functional site of alpha-dendrotoxin. The functional topographies of dendrotoxins are different but share a conserved core with those of other Kv1 potassium channelblocking toxins. J. boil. chem., 1998, 273(39), 25393-403.
254. Jiang, Y.; Lee, A.; Chen, J.; Ruta, V.; Cadene, M.; Chait, B. T.; MacKinnon, R. X-ray structure of a voltage-dependent K+ channel. Nature, 2003, 423(6935), 33-41.
255. Posson, D. J.; Ge, P.; Miller, C.; Bezanilla, F.; Selvin, P. R. Small vertical movement of a K+ channel voltage sensor measured with luminescence energy transfer. Nature, 2005, 436(7052), 848-51.
256. Cestèle, S.; Catterall, W. a. Molecular mechanisms of neurotoxin action on voltage-gated sodium channels. Biochimie, 2000, 82(9-10), 883-92.
257. Ujihara, S.; Oishi, T.; Torikai, K.; Konoki, K.; Matsumori, N.; Murata, M.; Oshima, Y.; Aimoto, S. Interaction of ladder-shaped polyethers with transmembrane??-helix of glycophorin A as evidenced by saturation transfer difference NMR and surface plasmon resonance. Bioorg. Med. Chem. Lett., 2008, 18(23), 6115-8.
258. Pedraza, Escalona M.; Possani, L. D. Scorpion beta-toxins and voltage-gated sodium channels: interactions and effects. Front. Biosci. (Landmark Ed), 2013, 18, 572-87.
259. Cestele, S.; Yarov-Yarovoy, V.; Qu, Y.; Sampieri, F.; Scheuer, T.; Catterall, W. A. Structure and function of the voltage sensor of sodium channels probed by a beta-scorpion toxin. J. Biol. Chem., 2006, 281(30), 21332-44.
260. Rogers, J. C.; Qu, Y.; Tanada, T. N.; Scheuer, T.; Catterall, W. A. Molecular Determinants of High Affinity Binding of-Scorpion Toxin and Sea Anemone Toxin in the S3-S4 Extracellular Loop in Domain IV of the Na+ Channel Subunit. J. Biol. Chem., 1996, 271(27), 15950-62.
261. Dias-Kadambi, B. L.; Drum, C. L.; Hanck, D. A.; Blumenthal, K. M. Leucine 18, a hydrophobic residue essential for high affinity binding of anthopleurin B to the voltage-sensitive sodium channel. J. Biol. Chem., 1996, 271(16), 9422-8.
262. Leipold, E.; Hansel, A.; Olivera, B. M.; Terlau, H.; Heinemann, S. H. Molecular interaction of delta-conotoxins with voltage-gated sodium channels. FEBS lett., 2005, 579(18), 3881-4.
263. Terlau, H.; Olivera, B. M. Conus venoms: a rich source of novel ion channel-targeted peptides. Physiol. Rev., 2004, 84(1), 41-68.
264. Winterfield, J. R.; Swartz, K. J. A hot spot for the interaction of gating modifier toxins with voltage-dependent ion channels. J. gen. physio., 2000, 116(5), 637-44.
265. Rogers, J. C.; Qu, Y.; Tanada, T. N.; Scheuer, T.; Catterall, W. A. Molecular determinants of high affinity binding of alpha-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the Na+ channel alpha subunit. j. biol. chem., 1996, 271(27), 15950-62.
266. Keith, R. A.; Mangano, T. J.; Lampe, R. A.; DeFeo, P. A.; Hyde, M. J.; Donzanti, B. A. Comparative actions of synthetic omegagrammotoxin SIA and synthetic omega-Aga-IVA on neuronal calcium entry and evoked release of neurotransmitters in vitro and in vivo. Neuropharmacology, 1995, 34(11), 1515-28.
267. Lampe, R.; Defeo, P.; Davison, M.; Young, J.; Herman, J.; Spreen, R.; Horn, M.; Mangano, T.; Keith, R. Isolation and pharmacological characterization of omega-grammotoxin SIA, a novel peptide inhibitor of neuronal voltage-sensitive calcium channel responses. Mol. Pharmacol., 1993, 44(2), 451-60.
268. Chuang, R. S.; Jaffe, H.; Cribbs, L.; Perez-Reyes, E.; Swartz, K. J. Inhibition of T-type voltage-gated calcium channels by a new scorpion toxin. Nat. Neurosci., 1998, 1(8), 668-74.
269. Olamendi-Portugal, T.; García, B. I.; López-González, I.; Van Der, Walt J.; Dyason, K.; Ulens, C.; Tytgat, J.; Felix, R.; Darszon, A.; Possani, L. D. Two new scorpion toxins that target voltage-gated Ca2+ and Na+ channels. Biochem. Biophys. Res. Commun., 2002, 299(4), 562-8.
270. Sidach, S. S.; Mintz, I. M. Kurtoxin a gating modifier of neuronal high-and low-threshold ca channels. J. Neurosci.: the official journal of the Society for Neuroscience, 2002, 22(6), 2023-34.
271. Stotz, S. C.; Spaetgens, R. L.; Zamponi, G. W. Block of voltagedependent calcium channel by the green mamba toxin calcicludine. J. memb. boil., 2000, 174(2), 157-65.
272. Wang, X.; Du, L.; Peterson, B. Z. Calcicludine binding to the outer pore of L-type calcium channels is allosterically coupled to dihydropyridine binding. Biochemistry, 2007, 46(25), 7590-8.
273. Hellwig, N.; Albrecht, N.; Harteneck, C.; Schultz, G.; Schaefer, M. Homo-and heteromeric assembly of TRPV channel subunits. J. Cell Sci., 2005, 118(Pt 5), 917-28.
274. Jara-Oseguera, A.; Llorente, I.; Rosenbaum, T.; Islas, L. D. Properties of the inner pore region of TRPV1 channels revealed by block with quaternary ammoniums. J. Gen. Physiol., 2008, 132(5), 547-62.
275. Moiseenkova-Bell, V. Y.; Stanciu, L. A.; Serysheva, II; Tobe, B. J.; Wensel, T. G. Structure of TRPV1 channel revealed by electron cryomicroscopy. Proc. Natl. Acad. Sci. U S A, 2008, 105(21), 7451-5.
276. Salazar, H.; Jara-Oseguera, A.; Hernandez-Garcia, E.; Llorente, I.; Arias, O. II; Soriano-Garcia, M.; Islas, L. D.; Rosenbaum, T. Structural determinants of gating in the TRPV1 channel. Nat. Struct. Mol. Biol., 2009, 16(7), 704-10.
277. Susankova, K.; Ettrich, R.; Vyklicky, L.; Teisinger, J.; Vlachova, V. Contribution of the putative inner-pore region to the gating of the transient receptor potential vanilloid subtype 1 channel (TRPV1). J. Neurosci., 2007, 27(28), 7578-85.
278. Changeux, J. P.; Kasai, M.; Lee, C. Y. Use of a snake venom toxin to characterize the cholinergic receptor protein. Proc. Natl. Acad. Sci. U S A, 1970, 67(3), 1241-7.
279. Eldefrawi, M. E.; Eldefrawi, A. T. Purification and molecular properties of the acetylcholine receptor from Torpedo electroplax. Arch. Biochem. Biophys., 1973, 159(1), 362-73.
280. Wilson, P. T.; Lentz, T. L.; Hawrot, E. Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica. Proc. Natl. Acad. Sci. U S A, 1985, 82(24), 8790-4.
281. Basus, V. J.; Song, G.; Hawrot, E. NMR solution structure of an alpha-bungarotoxin/nicotinic receptor peptide complex. Biochemistry, 1993, 32(46), 12290-8.
282. Samson, A.; Scherf, T.; Eisenstein, M.; Chill, J.; Angliste, J. The mechanism for acetylcholine receptor inhibition by alphaneurotoxins and species-specific resistance to alpha-bungarotoxin revealed by NMR. Neuron, 2002, 35(2), 319-32.
283. Zeng, H.; Moise, L.; Grant, M. A.; Hawrot, E. The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica. J. Biol. Chem., 2001, 276(25), 22930-40.
284. Harel M., Kasher R., Nicolas A., Guss J. M., Balass M., Fridkin M., Smit A. B., Brejc K., Sixma T. K., Katchalski-Katzir E., Sussman J. L., Fuchs S. The binding site of acetylcholine receptor as visualized in the X-Ray structure of a complex between alphabungarotoxin and a mimotope peptide. Neuron, 2001, 32(2), 265-75.
285. Dellisanti, C. D.; Yao, Y.; Stroud, J. C.; Wang, Z. Z.; Chen, L. Crystal structure of the extracellular domain of nAChR alpha1 bound to alpha-bungarotoxin at 1. 94 A resolution. Nat. Neurosci., 2007, 10(8), 953-62.
286. Huang, S.; Li, S. X.; Bren, N.; Cheng, K.; Gomoto, R.; Chen, L.; Sine, S. M. Complex between alpha-bungarotoxin and an alpha7 nicotinic receptor ligand-binding domain chimaera. Biochem. J., 2013, 454(2), 303-10.
287. Samson, A. O.; Levitt, M. Inhibition mechanism of the acetylcholine receptor by alpha-neurotoxins as revealed by normal-mode dynamics. Biochemistry, 2008, 47(13), 4065-70.
288. Hogg, R. C.; Hopping, G.; Alewood, P. F.; Adams, D. J.; Bertrand, D. Alpha-conotoxins PnIA and [A10L]PnIA stabilize different states of the alpha7-L247T nicotinic acetylcholine receptor. J. Biol. Chem., 2003, 278(29), 26908-14.
289. Kasheverov, I. E.; Zhmak, M. N.; Vulfius, C. A.; Gorbacheva, E. V.; Mordvintsev, D. Y.; Utkin, Y. N.; van Elk R., Smit A. B.; Tsetlin, V. I. Alpha-conotoxin analogs with additional positive charge show increased selectivity towards Torpedo californica and some neuronal subtypes of nicotinic acetylcholine receptors. FEBS J., 2006, 273(19), 4470-81.
290. Gao, B.; Harvey, P. J.; Craik, D. J.; Ronjat, M.; De, Waard M.; Zhu, S. Functional evolution of scorpion venom peptides with an inhibitor cystine knot fold. Biosci. Rep., 2013, 33(3).
291. Mouhat, S.; Andreotti, N.; Jouirou, B.; Sabatier, J. M. Animal toxins acting on voltage-gated potassium channels. Curr. Pharm. Des., 2008, 14(24), 2503-18.
292. Lee, C. W.; Kim, S.; Roh, S. H.; Endoh, H.; Kodera, Y.; Maeda, T.; Kohno, T.; Wang, J. M.; Swartz, K. J.; Kim, J. I. Solution structure and functional characterization of SGTx1, a modifier of Kv2. 1 channel gating. Biochemistry, 2004, 43(4), 890-7.
293. Posokhov, Y. O.; Gottlieb, P. A.; Morales, M. J.; Sachs, F.; Ladokhin, A. S. Is lipid bilayer binding a common property of inhibitor cysteine knot ion-channel blockers? Biophys. J., 2007, 93(4), L20-2.
294. Bae, C.; Sachs, F.; Gottlieb, P. A. The mechanosensitive ion channel Piezo1 is inhibited by the peptide GsMTx4. Biochemistry, 2011, 50(29), 6295-300.
295. Kamaraju, K.; Gottlieb, P. A.; Sachs, F.; Sukharev, S. Effects of GsMTx4 on bacterial mechanosensitive channels in inside-out patches from giant spheroplasts. Biophys. J., 2010, 99(9), 2870-8.
296. Bae, C.; Gottlieb, P. A.; Sachs, F. Human PIEZO1: removing inactivation. Biophys. J., 2013, 105(4), 880-6.
297. Pettersen, E. F.; Goddard, T. D.; Huang, C. C.; Couch, G. S.; Greenblatt, D. M.; Meng, E. C.; Ferrin, T. E. UCSF Chimera-a visualization system for exploratory research and analysis. J. Comput. Chem., 2004, 25(13), 1605-12.