μ-Conotoxin GIIIA interactions with the voltage-gated Na+ channel predict a clockwise arrangement of the domains

Journal of General Physiology

Volumn 116, Issue 5, 2000, Pages 679-689

  Dudley S C , Jr ^a   Chang, N ^a   Hall, J ^a   Lipkind, G ^a   Fozzard, H A ^a   French, R J ^a  

^a EMORY UNIVERSITY   (United States)

Author keywords

Binding sites; Electrophysiology; Kinetics; Molecular models; Site directed mutagenesis

Indexed keywords

ANTIARRHYTHMIC AGENT; ANTICONVULSIVE AGENT; ARGININE; GLUTAMINE; HYDROXYPROLINE; ION CHANNEL; LOCAL ANESTHETIC AGENT; LYSINE; METHIONINE; MU CONOTOXIN GIIIA; NEUROTOXIN; POLYPEPTIDE; SODIUM CHANNEL; THREONINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING SITE; CHANNEL GATING; CHEMICAL STRUCTURE; ELECTRIC ACTIVITY; MOLECULAR INTERACTION; NONHUMAN; PROTEIN DOMAIN; RAT; SITE DIRECTED MUTAGENESIS; SKELETAL MUSCLE;

AMINO ACIDS; ANIMALS; BINDING SITES; CALCIUM CHANNEL BLOCKERS; CONOTOXINS; ELECTROPHYSIOLOGY; KINETICS; MODELS, MOLECULAR; MUSCLE, SKELETAL; MUTAGENESIS, SITE-DIRECTED; PATCH-CLAMP TECHNIQUES; RATS; SODIUM CHANNELS;

EID: 0033756777     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.116.5.679     Document Type: Article

References (51)

1. + channel revealed by the NMR-derived structures of scorpion toxins
2. The signature sequence of voltage-gated potassium channels projects into the external vestibule
3. Synthesis and characterization of μ-conotoxin IIIa
4. Synthesis and characterization of an N-terminal-specific I-125-photoaffinity derivative of μ-conotoxin GIIIA which binds to the voltage-dependent sodium channel
5. Action of derivatives of μ-conotoxin GIIIA on sodium channels. Single amino acid substitutions in the toxin separately affect association and dissociation rates
6. Molecular motions within the pore of voltage-dependent sodium channels
7. Propagation of errors
8. Characterizing the μ-conotoxin binding site on voltage-sensitive sodium channels with toxin analogs and channel mutations
9. Extrapore residues of the loop of domain 2 of the voltage-gated skeletal muscle sodium channel (rSkM1) contribute to the μ-conotoxin GIIIA binding site
10. + channel localized by mutant cycle analysis
11. Chimeric study of sodium channels from rat skeletal and cardiac muscle
12. + channel revealed by cysteine mutagenesis
13. Conus geographus toxins that discriminate between neuronal and muscle sodium channels
14. + conduction and selectivity
15. + channel mutant: Possible localization of a binding site at the outer vestibule
16. On the choice of reference mutant states in the application of the double-mutant cycle method
17. I. Theory of protein engineering analysis of stability and pathway of protein folding
18. Interactions between a pore-blocking peptide and the voltage sensor of the sodium channel: An electrostatic approach to channel geometry
19. Agitoxin footprinting the Shaker potassium channel pore
20. A model relating the structure of the sodium channel to its function
21. + channel pore through mutant cycles with a peptide inhibitor
22. Strength and co-operativity of contributions of surface salt bridges to protein stability
23. Structure-activity relations of tetrodotoxin, saxitoxin and analogues
24. Tertiary structure of conotoxin GIIIA in aqueous solution
25. + channels revealed by cysteine mutagenesis
26. + channel
27. Probing a potassium channel pore with an engineered protonatable site
28. Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor
29. Mapping the receptor site for charybdotoxin, a pore-blocking potassium channel inhibitor
30. Structural conservation in prokaryotic and eukaryotic potassium channels
31. 3H]saxitoxin and μ-conotoxins
32. + channel
33. Diversity of conus neuropeptides
34. Solution structure of μ-conotoxin GIIIA analysed by 2D-NMR and distance geometry calculations
35. Mapping function to structure in a channel-blocking peptide: Electrostatic mutants of charybdotoxin
36. Structure of the sodium channel pore revealed by serial cysteine mutagenesis
37. Mechanisms of sodium/calcium selectivity in sodium channels probed by cysteine mutagenesis and sulfhydryl modification
38. + channel selectivity filter by mutant cycle-based structure analysis
39. Active site of μ-conotoxin GIIIA, a peptide blocker of muscle sodium channels
40. 2+-occupied conductance states of cardiac sodium channels modified by batrachotoxin: Exploring ion-ion interactions in a multi-ion channel
41. Pore properties of rat brain II sodium channels mutated in the selectivity filter domain
42. Energetics of protein-protein interactions: Analysis of the Barnase-Barstar interface by single mutations and double mutant cycles
43. Estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles
44. The μI skeletal muscle sodium channel: Mutation E403Q eliminates sensitivity to tetrodotoxin but not to μ-conotoxins GIIIA and GIIIB
45. + channels
46. Mapping the site of block by tetrodotoxin and saxitoxin of sodium channel-II
47. + channels is produced by a structural rearrangement of the outer vestibule
48. + channel pores revealed by single- and double-cysteine replacements
49. Structure-activity relationships of μ-conotoxin GIIIA: Structure determination of active and inactive sodium channel blocker peptides by NMR and simulated annealing calculations
50. Site-directed mutagenesis as a probe of enzyme structure and catalysis: Tyrosyl-tRNA synthetase cysteine-35 to glycine-35 mutation
51. Topology of the P segments in the sodium channel pore revealed by cysteine mutagenesis