Introduction

Enzyme Biocatalysis: Principles and Applications

Volumn , Issue , 2008, Pages 1-56

  Illanes, Andrés ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84889966360     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-1-4020-8361-7_1     Document Type: Editorial

References (451)

1. Abelson PH (1999) A potential phosphate crisis. Science 283(5410):2015
2. Abián O, Mateo C, Fernández-Lorente G et al. (2001) Stabilization of immobilized enzymes against water soluble organic cosolvents and generation of hyper-hydrophilic microenvironments surrounding enzyme molecules. Biocatal Biotransfor 19:489-504
3. Abián O, Grazú V, Hermoso J et al. (2004) Stabilization of penicillin G acylase from Escherichia coli: Site-directed mutagenesis of the protein surface to increase multipoint covalent attachment. Appl Env Microbiol 70(2):1249-1251
4. Abu-Reesh I, Faqir N (1996) Simulation of glucose isomerase reactor: Optimum operating temperature. Bioproc Eng 14:205-210
5. Adamczak M, Hari Krishna S (2004) Strategies for improving enzymes for efficient biocatalysis. Food Technol Biotechnol 42(4):251-264
6. Adams M, Kelly R (1998) Finding and using hyperthermophilic ezymes. TIBTECH 16:329-332
7. Adlercreutz P, Iborra JL, Schmid E et al. (1994) Applications. In: Cabral JMS, Boros DBL, Tramper J (eds). Applied biocatalysis. Harwood Acad Publ, Chur, pp 108-156
8. AehleW(2003) Enzymes in industry: Production and applications.Wiley-VCH, Weinheim, 484
9. Aguilera JM (1994) Identifying opportunities for engineering applications in agriculture, food and nutrition biochemistry in Latin America. In: Aguilera JM, San Martín R, Edwardson W (eds). Bioengineering and bioprocesses: Needs and opportunities in Latin America. Universidad de Santiago, Chile, pp 9-27
10. Aitken MD (1993) Waste treatment applications of enzymes: Opportunities and obstacles. Chem Eng J 52:B49-B58
11. Akbar U, Aschenbrenner CD, Harper MR et al. (2007) Direct solubilization of enzyme aggregates with enhanced activity in nonaqueous media. Biotechnol Bioeng 96(6):1030-1039
12. Alberghina L (2000) Protein engineering in industrial biotechnology. CRC Press, Boca Raton 376 pp. ISBN 9057024128
13. Alcalde M, Ferrera M, Plou FJ et al. (2006) Environmental biocatalysis: From remediation with enzymes to novel green processes. TIBTECH 24(6):281-287
14. Alexeeva M, Carr R, Turner NJ (2003) Directed evolution of enzymes: New biocatalysts for asymmetric synthesis Org Biomol Chem 1:4133-4137
15. Allen G, Lowe G (1973) Investigation of the active site of papain with fluorescent probes. Biochem J 133:679-686
16. Amorim-Fernandes J, Mcalphine M, Halling P (2005) Operational stability of subtilisin CLECs in organic solvents in repeated batch and continuous operation. Biochem Eng J 24(1):11-15
17. Anish R, Rahman MS, Rao M (2006) Application of cellulases from an alkalothermophilic Thermomonospora sp. in biopolishing of denims. Biotechnol Bioeng 96(1):48-56
18. Anonymous (1979) Nomenclature Committee of the International Union of Biochemistry (NC-IUB). Units of Enzyme Activity. Eur J Biochem 97:319-320
19. Anonymous (1984) Nomenclature Committee of IUB (NC-IUB) IUB-IUPAC Joint Commission on Biochemical Nomenclature (JCBN). J Biosc Rep 4(2):177-180
20. Arica MY (2000) Epoxy-derived pHEMA membrane for use bioactive macromolecules immobilization: Covalently bound urease in a continuous model system. J Appl Polym Sci 77(9):2000-2008
21. Arnold FH (2001) Combinatorial and computational challenges for biocatalyst design. Nature 409:253-257
22. Arnold FH, Moore JC (1997) Optimizing industrial enzymes by directed evolution. Adv Biochem Eng Biotechnol 58:1-14
23. Asano Y (2002) Overview of screening for new microbial catalysts and their uses in organic synthesis-selection and optimization of biocatalysts. J Biotechnol 94:65-72
24. Ashina Y, Suto M (1993) Development of an enzymatic process for manufacturing acrylamide and recent progress. In: Tanaka A, Tosa T, Kobayashi T (eds). Industrial application of immobilized biocatalysts. Marcel Dekker, New York pp 91-107
25. Aslanidis C, de Jong PJ (1990) Ligation-independent cloning of PCR products (LIC-PCR). Nucl Acid Res 18(20):6069-6074
26. Ayhan F, Rad AY, Ayhan H (2002) Biocompatibility investigation and urea removal from blood by urease-immobilized HEMA incorporated poly(ethyleneglycol dimethacrylate) microbeads. J Biomed Mat Res 64B(1):13-18
27. Baker JO, Ehrman CI, Adney WS et al. (1998) Hydrolysis of cellulose using ternary mixtures of purified cellulases. Appl Biochem Biotechnol (70):395-403
28. Ballesteros A, Bornscheuer U, Capewell A et al. (1995) Enzymes in non-conventional phases. Biocatal Biotransfor 13:1-42
29. Barberis S, Illanes A (1996) Catálisis enzimática en fase orgánica. Ing Quim March:165-173
30. Barberis S, Quiroga E, Arribére MC et al. (2002) Peptide synthesis in aqueous-organic biphasic systems catalyzed by a protease isolated from Morrenia brachystephana (Asclepiadaceae). J Mol Catal B: Enzym 17:39-47
31. Bardy SL, Ng SYM, Carnegie DS et al. (2005) Site-directed mutagenesis analysis of amino acids critical for activity of the type I signal peptidase of the archaeon Methanococcus voltae. J Bacteriol 187(3):1188-1191
32. Barros EJ, Wehtje E, Adlercreutz P (2000) Mass transfer studies on immobilized α-chymotrypsin biocatalysts prepared by deposition for use in organic medium. Biotechnol Bioeng 59:364-373
33. Barton LL, Georgi CE, Lineback DR (1972) Effect of maltose on glucoamylase formation by Aspergillus niger. J Bacteriol 111(3):771-777
34. Barton JW, Reed EK, Davison BH (1997) Gas-phase enzyme catalysis using immobilized lipase for ester production. Biotechnol Tech 11(10):747-750
35. Bartlett JMS, Stirling D (2003) PCR protocols. Humana Press, Totowa NJ USA, 556 pp. ISBN 0896036278
36. Barzana E, Klibanov A, Karel M (1987) Enzyme catalyzed, gas-phase reactions. Appl. Biochem Biotechnol 15:25-34
37. Barzana E, Karel M, Klibanov A (1989) Enzymatic oxidation of ethanol in the gaseous phase. Biotechnol Bioeng 34:1178-1185
38. Barzana E, García-Garibay M (1994) Production of fish protein concentrates. In: Martin AM (ed). Fisheries processing. Chapman Hall, London, pp 206-222
39. Basso A, De Martin L, Ebert C et al. (2000) High isolated yields in thermodynamically controlled peptide synthesis in toluene catalysed by thermolysin adsorbed on Cellite R-640. Chem Commun 467-468
40. Basso A, Spizzo P, Toniutti M (2006) Kinetically controlled synthesis of ampicillin and cepahalexin in highly condensed systems in the absence of a liquid aqueous phase. J Mol Catal B: Enzym 39:105-111
41. Bedford MR (2000) Exogenous enzymes in monogastric nutrition-Their current value and future benefits. Animal Feed Sci Technol 86(1):1-13
42. Bélafi-Bakó K, Nemestóthy N, Gubicza L (2004) A study on applications of membrane techniques in bioconversion of fumaric acid to L-malic acid. Desalin 162:301-306
43. Benkovic SJ, Ballesteros A (1997) Biocatalysts-The next generation. TIBTECH 15:385-386
44. Benković SJ, Hammes-Schiffer S (2003) A perspective on enzyme catalysis. Science 301(5637):1196-1202
45. Bhargava AK, Lalb H, Pundira CS (1999) Discrete analysis of serum uric acid with immobilized uricase and peroxidase J Biochem Biophys Meth 39(3):125-136
46. Bhosale SH, Rao MB, Deshpande VV (1996) Industrial aspects of glucose isomerase. Microbiol Rev 60:280-300
47. Bisswanger H (2004) Practical enzymology. Wiley VCH, Weinheim, 272
48. Björup P, Adlercreutz P, Clapés P (1999) Useful methods in enzymatic synthesis of peptides. A comparative study focussing on kinetically controlled synthesis of Ac-Phe-Ala-NH2 catalyzed by α-chymotripsin. Biocatal Biotransfor 17:319-345
49. Black M, Miller R (2006) Platform chemicals from crops. J Chem Technol Biotechnol 81(11):1725-1728
50. Boersma YL, Dröge MJ, Quax WJ (2007) Selection strategies for improved biocatalysts. FEBS J 274 (9):2181-2195
51. Boidin A, Effront J (1917) Process manufacturing diastases and toxins by oxidizing ferments. US Patent 1227525
52. Boller T, Meier C, Menzler S (2002) Eupergit oxirane acrylic beads: How to make enzymes fit for biocatalysis. Org Proc Res Dev 6(4):509-519
53. Bommarius AS, Riebel BR (2004) Biocatalysis: Fundamentals and applications. Wiley-VCH, Weinheim, Germany, 611 pp. ISBN 3527303448
54. Bommarius AS, Broering JM (2005) Established and novel tools to investigate biocatalyst stability. Biocatal Biotransfor 23(3/4):125-139
55. Bordusa F (2002) Proteases in organic synthesis. Chem Rev 102:4817-4867
56. Bornscheuer UT, Kazlauskas RJ (1999) Hydrolases in organic synthesis. Wiley VCH, Weinheim, 336
57. Bowers LD (1986) Applications of immobilized biocatalysts in chemical analysis Anal. Chem 58(4):513-530
58. Brakmann S, Johnsson K (eds) (2002) Molecular evolution of proteins: Or how to improve enzymes for biocatalysis. Wiley-VCH, Weinheim, 368
59. Brenner S, Johnson M, Bridgham J et al. (2000) Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays. Nat Biotechnol 18:630-634
60. Brink LES, Tramper J, Luyben KCAM et al. (1988) Biocatalysis in organic media. Enzyme Microb Technol 10:736-743
61. Bruggink A (2001) Synthesis of β-lactam antibiotics, Kluwer Acad Publ, Dordrecht, 335
62. Bruggink A, Straathof AJJ, van der Wielen LAM (2003) Fine chemical industry for life science products: Green solutions to chemical challenges. In: Advances in biochemical engineering/ biotechnology, vol 80. Springer-Verlag, Berlin, Heidelberg, New York, pp 69-113
63. Brühlmann F (1995) Purification and characterization of an extracellular pectate lyase from an Amycolota sp. Appl Environ Hicrobiol 61(10):3580-3585
64. Bryjak J, Aniulyte J, Liesiene J (2007) Evaluation of man-Tailored cellulose-based carriers in glucoamylase immobilization. Carbohyd Res 342(8):1105-1109
65. Buchholz K, Kasche V, Bornscheuer UT (2005) Biocatalysts and enzyme technology.Wiley VCH, Weinhein, 448
66. Bucke C (1996) Oligosaccharide synthesis using glycosidases. J Chem Technol Biotechnol 67:217-220
67. Cammarota MC, Freire DMG (2006) A review on hydrolytic enzymes in the treatment of wastewater with high oil and grease content. Biores Technol 97(17):2195-2210
68. Canevascini G, Gattlen C (1981) A comparative investigation of various cellulase assay procedures. Biotechnol Bioeng 23(7):1573-1590
69. Cao L, Fischer A, Bornscheuer UT et al. (1997) Lipase-catalysed solid phase synthesis of sugar fatty acid esters. Biocatal Biotransfor 14:269-283
70. Cao L, van Langen L, Sheldon, R (2003) Immobilised enzymes: Carrier-bound or carrier-free? Curr Opin Biotechnol 14:1-8
71. Carasik W, Carroll OJ (1983) Development of immobilized enzymes for production of highfructose corn syrup. Food Technol 37(10):85-91
72. Caridis KA, Papathanasiou TD (1995). The dynamic performance of an immobilised-urease bioreactor in a recycle loop Bioproc Eng 14(1):41-50
73. Carrea G, Riva S (2000) Properties and synthetic applications of enzymes in organic solvents. Angew Chem Int Ed 39:2226-2254
74. Castang S, Shevchik VE, Hugouvieux-Cotte-Pattat N et al. (2004) Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative. Acta Cryst 60:190-192
75. Castro GR (1999) Enzymatic activities of proteases dissolved in organic solvents. Enzyme Microb Technol 25:689-694
76. Castro GR, Knubovets T (2003) Homogeneous biocatalysis in organic solvents and water-organic mixtures. Critic Rev Biotechnol 23(3):195-231
77. Castro M, Cabral J (1989) Kinetic studies of hydrogenase in AOT reverse micelles. Enzyme Microb Technol 11(6-11
78. Castro RC (2000) Properties of soluble α-chymotrypsin in neat glycerol and water. Enzyme Microb Technol 27:143-150
79. Chaiwut P, Kanasawud P, Halling PJ (2007) Solid-To-solid peptide synthesis by glycyl endopeptidase. Enzyme Microb Technol 40(4):954-960
80. Cheetham PSJ (1994) Case studies in applied biocatalysis-from ideas to products. In: Cabral JMS, Boros DBL, Tramper J (eds). Applied biocatalysis. Harwood Acad Publ, Chur, pp 47-108
81. Chen DH, Leu JC, Huang TC (1994) Transport and hydrolysis of urea in a reactor-separator combining an anion-exchange membrane and immobilized urease. J Chem Technol Biotechnol 61(4):351-357
82. Chen JP, Wang HY (1998) Improved properties of bilirubin oxidase by entrapment in alginate-silicate sol-gel matrix. Biotechnol Tech 12(11):851-853
83. Chen Y, Kang ET, Neoh KG et al. (2000) Covalent immobilization of invertase onto the surfacemodified polyaniline from graft copolymerization with acrylic acid. Eur Polym J 36(10):2095-2103
84. Chen R (2001) Enzyme engineering: Rational design versus directed evolution. TIBTECH 19(1):13-14
85. Chibata I, Tosa T, Sato T (1974) Process for the production of L-Aspartic acid. US Patent 3791926. Issued on February 12, 1974
86. Chibata I, Tosa T, Sato T (1987) Application of immobilized biocatalysts in pharmaceutical and chemical industries. In: Rehm HJ, Reed G (eds). Biotechnology, vol. 7a. Verlag Chemie, Weinhein, pp 653-684
87. Cho H, Adrio JL, Luengo JM et al. (1998) Elucidation of conditions allowing conversion of penicillin G and other penicillins to deacetoxycephalosporins by resting cells and extracts of Streptomyces clavuligerus NP1. Proc Natl Acad Sci USA 95(20):11544-11548
88. Choct M, Hughes RJ, Trimble RP et al. (1995) Non-starch polysaccharide-degrading enzymes increase the performance of broiler chickens fed wheat of low apparent metabolizable energy. J Nutr 125:485-492
89. Chodorge M, Fourage L, Ullmann C et al. (2005) Rational strategies for directed evolution of biocatalysts-Application to Candida antarctica lipase B (CALB) Adv Synth Catal 347:1022-1026
90. Christensen H, Thyssen HH, Schebye O et al. (1990) Three highly sensitive "bedside" serum and urine tests for pregnancy compared. Clinic Chem 36:1686-1688
91. Christie RB (1980) The medical use of proteolytic enzymes. In:Wiseman A (ed). Topics in enzyme and fermentation biotechnology 4. Ellis Horwood, Chichester, pp 25-84
92. Cianci JJ (1986) The market outlook for enzymes. In: Bioprocessing. The world biotech report, vol 2. Part 3. Online International Inc, New York, pp 55-72
93. Clark DS (2004) Characteristics of nearly dry enzymes in organic solvents: Implications for biocatalysis in the absence of water. Philos Trans R Soc Lond B Biol Sci 359:1299-1307
94. Colby TD, Bahnson BJ, Chin JK et al. (1998) Active site modifications in a double mutant of liver alcohol dehydrogenase: Structural studies of two enzyme-ligand complexes. Biochem 37(26):9295-9304
95. Comfort AR, Albert EC, Langer R (1989) Immobilized enzyme cellulose hollow fibers: Immobilization of heparinase Biotechnol Bioeng 34(11):1366-1373
96. Convents A, Busch A, Baeck AC (1995) Detergent compositions with high activity cellulase and softening clays. US Patent 5443750. Issued on August 22, 1995
97. Cooperband LR, Good LW (2002) Biogenic phosphate minerals in manure: Implications for phosphorus loss to surface waters. Environ Sci Technol 36:5075-5082
98. Coulon D, Ghoul M (1998) The enzymatic synthesis of non-ionic surfactants: The sugar esters-An overview. Agro Food Ind Hi-Tech (July/August):22-26
99. Coutinho P, Reilly PJ (1997) Glucoamylase structural functional and evolutionary relationships. Proteins 29:334-347
100. Cowan D (1996) Industrial enzyme technology. TIBTECH 14:177-178
101. Crabb WD, Mitchinson C (1997) Enzymes involved in the processing of starch to sugars. TIBTECH 15:349-352
102. Crabb WD, Shetty J (1999) Commodity scale production of sugars from starches. Curr Opin Microbiol 2:252-256
103. Creighton TE (1993) Proteins 2nd edn. W.H Freeman and Company, New York, 507
104. Dai C, Wang B, Zhao H (2005) Microencapsulation peptide and protein drugs delivery system. Colloids Surf B Biointerf 41(2-3):117-120
105. Danielsson B (1987) The enzyme thermistor: A versatile instrument for measurements on enzymes, substrates, and cells. Ann NY Acad Sci 501(1):543-544
106. Davis B (2003) Chemical modification of biocatalysts. Curr Opin Biotechnol 14:379-386
107. Davis BG, Boyer V (2001) Biocatalysis and enzymes in organic synthesis. Nat Prod Rep 18:618-640
108. Davis M (1998) Making a living at the extremes. TIBTECH 16:102-104
109. Declerck N, Jiyet D, Trosset JY et al. (1995) Hyperthermostable mutants of Bacillus licheniformis alpha amylase: Multiple amino acid replacements and molecular modelling. Protein Eng 8:1029-1037
110. Declerck N, Machius M, Joyet P et al. (2003) Hyperthermostabilization of Bacillus licheniformis α-Amylase and modulation of its stability over a 50°C temperature range Prot Eng 16(4):287-293
111. Deetz JA, Rozzell J (1988) Enzyme-catalysed reactions in non-Aqueous media. TIBTECH 6:15-19
112. Demirjian DC, Moris-Varas F, Cassidy CS (2001) Enzymes from extremophiles. Curr Opin Chem Biol. 5(2):144-51
113. Dickinson M, Fletcher P (1989) Enzymes in organic solvents. Enzyme Microb Technol 11:55-56
114. Disioudi B, Grimsley JK, Lai K et al. (1999) Modification of near active site residues in organophosphorus hydrolase reduces metal stoichiometry and alters substrate specificity. Biochem 38(10):2866-2872
115. Demain AL (1968) Regulatory mechanisms and the industrial production of microbial metabolites. Lloydia 31(4):395-418
116. Dixon M, Webb EC (1979) Enzymes 3rd ed. Academic Press, New York, 1116
117. Drauz K, Waldmann H (2002) Enzyme catalysis in organic synthesis, vol II. Wiley VCH, Weinhein, Germany, 998
118. Dravis BC, LeJeune KE, Hetro AD et al. (2000) Enzymatic dehalogenation of gas phase substrates with haloalkane dehalogenase. Biotechnol Bioeng 69(3):235-241
119. D'Souza S (1999) Immobilized enzymes in bioprocess. Curr Sci 77:69-78
120. Durand J, Teuma E, Gómez M (2007) Ionic liquids as a medoium for enantioselective catalysis. C R Chimie 10:152-177
121. Duval M, Suciu S, Ferster A et al. (2002) Comparison of Escherichia coli-Asparaginase with Erwinia-Asparaginase in the treatment of childhood lymphoid malignancies: Results of a randomized European Organisation for Research and Treatment of Cancer-Children's Leukemia Group phase 3 trial. Blood 99(8):2734-2739
122. Ebert C, Gardossi L, Linda P et al. (1996) Influence of organic solvents on enzyme chemoselectivity and their role in enzyme-substrate interaction. Tetrahedron 52:4867-4876
123. Eijsink VGH, Bjǿrk A, G̊aseidnes S et al. (2004) Rational engineering of enzyme stability. J Biotechnol 113:105-120
124. Eisenthal R, Peterson ME, Daniel RM et al. (2006) The thermal behaviour of enzyme activity: Implications for biotechnology. TIBTECH 24(7):289-292
125. Endo J, Tabata M, Okada S, Murachi T (1979) Use of immobilized enzymes in automated clinical analysis: Determination of uric acid and glucose using immobilized enzymes in column form. Clin Chim Acta 95(2):411-417
126. Enfors SO, Molin N (1978) Enzyme electrodes for fermentation control. Proc Biochem 13:9-24
127. Erbeldinger M, Ni X, Halling PJ (1998) Effect of water and enzyme concentration on thermolysincatalyzed solid-To-solid peptide synthesis. Biotechnol Bioeng 59(1):68-72
128. Eriksson KEL, Akhtar M, Argyropoulos DS et al. (1997) Biotechnology in the pulp and paper industry. Advances in biochemical engineering biotechnology 57. Springer, Berlin, Heidelberg, New York, 339
129. Eschenbrenner E, Covès J, Fontecave M (1995) The flavin reductase activity of the flavoprotein component of sulfite reductase from Escherichia coli. J Biol Chem 270(35):20550-20555
130. Faber K (1997) Biotransformations in organic chemistry. Springer, Berlin, Heidelberg, New York, 402 pp. ISBN 3540616888
131. Fang TY, Ford C (1998) Protein engineering of Aspergillus awamori glucoamylase to increase its pH optimum. Protein Eng 11:383-388
132. Faqir N, Abu-Reesh I (1998) Optimum temperature operation mode for glucose isomerase operating at constant glucose conversion. Bioproc Eng 19:11-17
133. Fonseca M.J, Jagtenberg JC, Haisma HJ et al. (2003) Liposome-mediated targeting of enzymes to cancer cells for site-specific activation of prodrugs: Comparison with the corresponding antibody-enzyme conjugate. Pharm Res 20(3)423-428
134. Foody B, Tolan JS (1999) Method and enzyme mixture for improved depilling of cotton goods. United States Patent 5866407. Issued on February 2, 1999
135. Fowler T, Brown RD (1992) The bgl1 gene encoding extracellular beta-glucosidase from Trichoderma reesei is required for rapid induction of the cellulase complex. Mol Microbiol 6(21):3225-3235
136. Furui M, Sakata N, Otsuki O et al. (1988) A bioreactor-crystallizer for L-malic acid production. Biocatal Biotransfor 2(1):69-77
137. Fusee MC (1987) Industrial production of L-Aspartic acid using polyurethane-immobilized cells containing aspartase. Methods Enzymol 136:463-471
138. Garcia S, Lourenço NMT, Lousa D et al. (2004) A comparative study of biocatalysis in nonconventional solvents: Ionic liquids, supercritical fluids and organic media. Green Chem 6:466-470
139. García-Junceda E, García-García JF, Bastida A et al. (2004) Enzymes in the synthesis of bioactive compounds: The prodigious decades. Bioorg Med Chem 12:1817-1834
140. Gaseidnes S, Synstad B, Nielsen JE et al. (2003) Rational engineering of the stability and the catalytic performance of enzymes. J Mol Catal B: Enzym 21:3-8
141. Gekas V, López-Leiva M (1985) Hydrolysis of lactose: A literature review. Proc Biochem 20(1):2-12
142. Gill I, López-Fandiño R, Jorba X et al. (1996) Biologically active peptides and enzymatic approaches to their production. Enzyme Microb Technol 18:162-183
143. Godbole S, Decker SR, Nieves RA et al. (1999) Cloning and expression of Trichoderma reesei CBH I in Pichia pastoris. Biotechnol Prog (15(3):828-833
144. Gomes J, Steiner W (2004) The biocatalytic potential of extremophiles and extermozymes. Food Technol Biotechnol 42(4):223-235
145. Gómez-Puyou MT, Gómez-Puyou A (1998) Enzymes in low water systems. Crit Rev Biochem Mol Biol 33:53-89
146. Gorbhel B, Sellami-Kamoun A, Nasri M (2003) Stability studies of protease from Bacillus cereus BG1. Enzyme Microb Technol 32:513-518
147. Gorton L, Csoregi E, Dominguez E et al. (1991) Selective detection in flow analysis based on the combination of immobilized enzymes and chemically modified electrodes Anal Chim Acta 250(1):203-248
148. Goto CE, Barbosa EP, Kistner LCL et al. (1998) Production of amylase by Aspergillus fumigatus utilizing α-methyl-D-glycoside, a synthetic analogue of maltose, as substrate. FEMS Microbiol Lett 167(2):139-143
149. Gray KA, Zhao L, Emptage M (2006) Bioethanol. Curr Opin Chem Biol 10(2):141-146
150. Greenberg NA, Mahoney RR (1981) Immobilisationof lactase (β-galactosidase) for use in dairy processing: A review. Proc. Biochem 16(2):2-49
151. Greenberg M (1996) Nutritional formula. US Patent 5569458. Issued on Oct 29, 1996
152. Gulati R, Isar J, Kumar V et al. (2005) Production of a novel alkaline lipase by Fusarium globulosum using neem oil, and its applications. Pure Appl Chem 77(1):251-262
153. Gupta MN, Roy I (2004) Enzymes in organic media: Forms, functions and applications Eur J Biochem 271:2573-2583
154. Gupta R, Gupta K, Saxena RK et al. (1999) Bleach-stable, alkaline protease from Bacillus sp. Biotechnol Lett 21(2):135-138
155. Gupta R, Beg QK, Lorenz P (2002) Bacterial alkaline protease: Molecular approaches and industrial applications. Appl Microbiol Biotechnol 59:15-32
156. Gusakov AV, Markov AV, Grishutin SG et al. (2002) Viscometric method for assaying of total endodepolymerase activity of pectinases. J Biochem (Moscow) 67(6):676-682
157. Gutiérrez A, del Rió JC, Martínez MJ et al. (2001) The biotechnological control of pitch in paper pulp manufacturing. TIBTECH 19:340-348
158. Guzmán F, Barberis S, Illanes A (2007). Peptide synthesis: Chemical or enzymatic. Electron J Biotechnol 10(2):279-314
159. Haki GD, Rakshit SK (2003) Developments in industrially important thermostable enzymes: A review. Biores Technol 89(1):17-34
160. Halldórsdóttir S, Thórólfsdóttir E, Spilliaert R et al. (1998) Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosilhydrolase family 12. Appl Microbiol Biotechnol 49:277-284
161. Halling PJ (2004) What can we learn by studying enzymes in non-Aqueous media? Philos Trans R Soc Lond B Biol Sci 359:1287-1296
162. Han MS, Jung SO, Kim MJ et al. (2004) Fluorometric assay protocol for protease-catalyzed transesterification reactions in organic solvents J Org Chem 69(8):2853-2855
163. Hanbin M, Yang T, Cremer PS (2002) Design and characterization of immobilized enzymes in microfluidic systems. Anal Chem 74:379-385
164. Hari Krishna S (2002) Developments and trends in enzyme catalysis in non-conventional media. Biotechnol Adv 20:239-267
165. Häring D, Schreier P (1999) Cross-linked enzyme crystals. Curr Opin Chem Biol 3:35-38
166. Hasan F, Shah AA, Hameed A (2006) Industrial applications of microbial lipases. Enzyme Microb Technol 39:235-251
167. Haschemeyer RH, Haschemeyer AEV (1973). Proteins: A guide to study by physical and chemical methods, John Wiley, New York, 358
168. Hemmingsen S (1979) Development of an immobilized glucose isomerase for industrial applications. In: Wingard L, Katchalski-Katzir E, Goldstein L (eds). Applied biochemistry and bioengineering, vol 2. Academic Press, New York, pp 157-182
169. Heyman MB (2006) Lactose intolerance in infants, children, and adolescents. Pediatrics 118(3):1279-1286
170. Hodgson J (1994) The changing bulk biocatalyst market. Biotechnol 12:789-790
171. Hoffmeister HM, Szabo S, Kastner C et al. (1998) Thrombolytic therapy in acute myocardial infarction: Comparison of procoagulant effects of streptokinase and alteplase regimens with focus on the kallikrein system and plasmin. Circulation 98:2527-2533
172. Hoondal G, Tiwari R, Dahiya N et al. (2002) Microbial alkaline pectinases and their industrial applications: A review. J Appl Microbiol Biotechnol 59(4-5):409-418
173. Hudson EP, Eppler RK, Clark DS (2005) Biocatalysis in semi-Aqueous and nearly anhydrous conditions. Curr Opin Biotechnol 16:637-643
174. Huisman GW, Gray D (2002) Towards novel processes for the fine-chemical and pharmaceutical industries. Curr Opin Biotechnol 13(4):352-358
175. Hultin HO (1983) Current and potential uses of immobilized enzymes. Food Technol 37(10):66-82
176. Hummel W (1999) Large-scale applications of NAD(P)-dependent oxidoreductases: Recent developments. TIBTECH 17:487-493
177. Illanes A (1999) Stability of biocatalysts. Elect J Biotechnol 2:1-9
178. Illanes A (2000) Biocatalysts for the food industry. In: Lozano JE, Añón C, Parada-Arias E, Barbosa-Cánovas GV (eds). Trends in food engineering. Technomic Publ, Co, Lancaster, pp 287-298
179. Illanes A, Fajardo A (2001) Kinetically controlled synthesis of ampicillin with immobilized penicillin acylase in the presence of organic cosolvents. J Mol Catal B Enzym 11:587-595
180. Illanes A, Rossi MC (1980) Inducción de celulasas de Trichoderma reesei en medios de cultivo definidos. Rev Arg Microbiol 12:79-86
181. Illanes A, Schaffeld G (1983) Protein enrichment of treated and untreated leached beet cosette. Biotechnol Lett 5(5):305-310
182. Illanes A, Gentina JC, Marchese MP (1988) Production and stabilization of cellulases from Trichoderma reesei. MIRCEN J Appl Microbiol Biotechnol 4:407-414
183. Illanes A, Ruiz A, Zuñiga M et al. (1990) Immobilization of lactase for the continuous hydrolysis of whey permeate. Bioproc Eng 5:257-262
184. Illanes A, Zuñiga ME, Contreras S et al. (1992) Reactor design for the enzymatic isomerization of glucose to fructose. Bioproc Eng 7:199-204
185. Illanes A, Ruiz A, Zuñiga ME (1993) Análisis comparativo de dos lactasas microbianas inmovilizadas. Alimentos 18(1):26-34
186. Illanes A, Wilson L, Raiman L (1999) Design of immobilized enzyme reactors for the continuous production of fructose syrup from whey permeate. Bioproc Eng 21:509-515
187. Illanes A, Wilson L, Tomasello G (2000) Temperature optimization for reactor operation with chitin-immobilized lactase under modulated inactivation. Enzyme Microb Technol 27:270-278
188. Illanes A, Anjari S, Altamirano C et al. (2004) Optimization of cephalexin synthesis with immobilized penicillin acylase in ethylene glycol medium at low temperatures. J Mol Catal B: Enzym 30:95-103
189. Illanes A, Wilson L, Caballero E et al. (2006) Crosslinked penicillin acylase aggregates for synthesis of β-lactam antibiotics in organic medium. Appl Biochem Biotechnol 133:189-202
190. Ijima Y, Matoishi K, Terao Y et al. (2005) Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism. Chem Commun 877-879
191. Ikeda K, Schiltz E, Fujii T et al. (2005) Phenylalanine ammonia-lyase modified with polyethylene glycol: Potential therapeutic agent for phenylketonuria. Amino Acids 29(3):283-287
192. Imanaka T, Shibazaki M, Takagi M (1986) A new way of enhancing the thermostability of proteases Nature 324:695-697
193. International Union of Biochemistry (1965) Enzyme nomenclature, recommendations 1964 of the International Union of Biochemistry, Elsevier, Amsterdam
194. Ioannou YA, Zeidner KM, Gordon RE et al. (2001) Fabry disease: Preclinical studies demonstrate the effectiveness of α-galactosidase replacement in enzyme-deficient mice Amer J Human Gen 68:14-25
195. Jaeger KE, Eggert T (2002) Lipases for biotechnology. Curr Opin Biotechnol 13(4):390-397
196. Jianrong C, Yuqing M, Nongyueb H et al. (2004) Nanotechnology and biosensors. Biotechnol Adv 22(7):505-518
197. Joyet P, Declerck N, Gaillardin C (1992) Hyperthermostable variants of a highly thermostable alpha-Amylase. Bio/Technology 10:1579-1583
198. Józefiak D, Rutkowski A, Jensen BB et al. (2006) The effect of beta-glucanase supplementation of barley- and oat-based diets on growth performance and fermentation in broiler chicken gastrointestinal tract. Brit Poult Sci 47(1):57-64
199. Kallenberg A, van Rantwijk F, Sheldon R (2005) Immobilization of penicillin G acylase: The key to optimum performance. Adv Synth Catal 347:905-926
200. Kamat S, Beckman E, Russell, A (1995) Enzyme activity in fluids. Critic Rev Biotechnol 15:41-71
201. Kanerva LT, Klibanov AM (1989) Hammett analysis of enzyme action in organic solvents. J Amer Chem Soc 111:6864-6865
202. Karma J, Nicell JA (1997) Potential applications of enzymes in waste treatment. J Chem Technol Biotechnol 69:141-153
203. Kashyap DR, Vohra PK, Chopra S et al. (2001) Applications of pectinases in the commercial sector: A review Biores. Technol 77(3):215-227
204. Katchalsky-Katzir E (1993) Immobilized enzymes. Learning from past successes and failures. TIBTECH 11:471-478
205. Kaur J, Sharma R (2006) Directed evolution: An approach to engineer enzymes. Crit Rev Biotechnol 26:165-199
206. Kaur P, Kunze G, Satyanarayana T (2007) Yeast phytases: Present scenario and future perspectives. Critic Rev Biotechnol 27:93-109
207. Kawashiro K, Sugahara H, Sugiyama S (1997) Effect of organic solvents on enantioselectivity of protease catalysis. Biotechnol Bioeng 53:26-31
208. Kim J, Grate JW, Wang P (2006) Nanostructures for enzyme stabilization. Chem Eng Sci 61(3):1017-1026
209. Kirk Wright S, Viola RE (2001) Alteration of the specificity of malate dehydrogenase by chemical modulation of an active site arginine. J Biol Chem 276(33):31151-311551
210. Klein MD, Langer R (1986) Immobilized enzymes in clinical medicine: An emerging approach to new drug therapies. TIBTECH 4:179-186
211. Klein AE, Freiberg J, Same S et al. (1989) Rapid colorimetric determination of activity of subtilisin enzymes in cleaning products. Assoc Off Anal Chem 72(6):881-882
212. Klibanov AM (1977) A new approach to preparative enzymatic synthesis. Biotechnol Bioeng 28:417-421
213. Klibanov AM (1983) Stabilization of enzymes against thermal inactivation. Adv Appl Microbiol 29:1-28
214. Klibanov AM (1986) Enzymes that work in organic solvents. Chem Technol 6:354-359
215. Klibanov AM (1989) Enzymatic catalysis in anhydrous organic solvents. TIBTECH 14(4):141-144
216. Klibanov AM (1997) Why are enzymes less active in organic solvents than in water? TIBTECH 15:97-101
217. Klibanov A (2001) Improving enzymes by using them in organic solvents. Nature 409:241-246
218. Knez Z, Laudani CG, Habulin M et al. (2007) Exploiting the pressure effect on lipase-catalyzed wax ester synthesis in dense carbon dioxide. Biotechnol Bioeng 97(6):1366-1375
219. Kobayashi M, Nagasawa T, Yamada H (1995) Enzymatic synthesis of acrylamide: A success story not yet over. TIBTECH 10:402-408
220. Kodama S (1996) Optimal conditions for effective use of acid urease in wine. J Food Sci 61(3):548-552
221. Koeller KM, Wong CH (2001) Enzymes for chemical synthesis. Nature 409:232-240
222. Koshland DE (1958) Application of a theory of enzyme specificity to protein synthesis. Proc Natl Acad Sci USA 44(2):98-104
223. Koskinen AMP, Klibanov AM (1996) Enzymatic reactions in organic media. Blackie Academic & Professional, London, 314
224. Kovalenko GA, Perminova LV, Terent'eva TG et al. (2007) Catalytic properties of glucoamylase immobilized on synthetic carbon material Sibunit. J Appl Biochem Microbiol 43(4):374-378
225. Krackhardt A, Schwartz S, Thiel E (2000) Targeting folates by carboxypeptidase G2: Potential applications in anticancer therapy. Onkologie 23(6):538-543
226. Krieger N, Bhatnagar T, Baratti J et al. (2004) Non-Aqueous biocatalysis in heterogeneous solvent systems. Food Technol Biotechnol 42(4):279-286
227. Kuhl P, Jakubke HD (1990) Protease-catalyzed synthesis of peptides in biphasic aqueous-organic systems. Pharmazie 45:393-400
228. Kula MR (2002) Cofactors and coenzymes. In: Drauiz K, Waldmann H (eds). Enzyme catalysis in organic synthesis. Wiley-VC, Weinhein, pp 12-20
229. Laane C, Boeren S, Vos K et al. (1987) Rules for optimisation of biocatalysis in organic solvents. Biotechnol Bioeng 30:81-87
230. Lamare S, Legoy M (1993) Biocatalysis in the gas phase. TIBTECH 11:413-418
231. Laudani CG, Habulin M, Knez Ž et al. (2007) Lipase-catalyzed long chain fatty ester synthesis in dense carbon dioxide: Kinetics and thermodynamics. J Supercritical Fluids 41:92-101
232. Lauwers A, Scharpé S (1997) Pharmaceutical enzymes. Marcel Dekker, New York, 401
233. Laval JM, Thomas D, Mazeran PE (2000) Nanobiotechnology and its role in the development of new analytical devices. Analyst 125:29-3
234. Law BA (2002) Enzymes in the manufacture of dairy products. In: Whitehurst RJ, Law BA (eds). Enzymes in food technology. CRC Press, Boca Raton, pp 76-10
235. Lawung R, Danielsson, Prachayasittiku V et al. (2001) Calorimetric analysis of cephalosporins using an immobilized TEM-1 b-lactamase on Ni21chelating Sepharose fast flow. Anal Biochem 296:57-62
236. Lee CM, Bagdasarian M, Zeikus JG (1990) Characterization of Thermoanaerobacter glucose isomerase in relation to saccharidase synthesis and development of single-step process for sweetener production. Appl Environ Microbiol 56:2895-2901
237. Lee MY, Dordick JS (2002) Enzyme activation for nonaqueous media. Curr Opin Biotechnol 13(4):376-384
238. Lehninger A (1970) Biochemistry. Worth Publ Inc, New York, 833
239. Leal MCMR, Cammarota MC, Freire MG et al. (2002) Hydrolytic enzymes as coadjuvants in the anaerobic treatment of dairy wastewaters. Braz J Chem Eng 19(2):175-180
240. Leisola M, Turunen O (2007) Protein engineering: Opportunities and challenges. Appl Microbiol Biotechnol 75(6):1225-1232
241. Leuchtenberger W, Huthmacher K, Drauz K (2005) Biotechnological production of amino acids and derivatives: Current status and prospects. J Appl Microbiol Biotechnol 69(1):1-8
242. Levitsky VY, Lozano P, Iborra JL (1999) Kinetic analysis of deactivation of immobilized α-chymotripsin by water-miscible organic solvent in kyotorphin synthesis. Biotechnol Bioeng 65:170-175
243. Liang JF, Li YT, Yang VC (2000) Biomedical application of immobilized enzymes. J Pharm Sci 89(8):979-990
244. Liese A, Seelbach K, Buchholz A et al. (2006) Lyases. In: Liese A, Seelbach K, Wandrey C (eds). Industrial biotransformations, 2nd edn. Wiley-VCH Verlag, Weinheim, pp 447-503
245. Lima UA, Basso LC, de Amorim HV (2001) Producǎo de etanol. In: Lima UA, Aquarone E, Borzani W, Schmidell W (ed). Biotecnologia industrial. Edgard Blücher, Sao Paulo, pp 1-44
246. Liu SY,Wiegel J, Gherardini FC (1996) Purification and cloning of a thermostable xylose (glucose) isomerase with an acidic ph optimum from Thermoanaerobacterium strain JW/SL-YS 489. J Bacteriol 178(20):5938-5945
247. Liu YY, Lou WY, Zong MH (2005) Increased enantioselectivity in the enzymatic hydrolysis of amino acid esters in the ionic liquid 1-butyl-3-methyl-imidazolium. Biocatal Biotransfor 23(2):89-95
248. Longo MA, Meynial I, Combes D (1995) Chemical and enzymatic glycosylation of enzymes. Modification of their properties. Ann NY Acad Sci 750(1):125-129
249. López C, Moreira MT, Feijoo G et al. (2004). Dye decolorization by manganese peroxidase in an enzymatic membrane bioreactor. Biotechnol Progs 20:74-81
250. Lowe CR (1989) Biosensors. Philos Trans R Soc Lond B Biol Sci 324(1224):487-496
251. Lübbe AS, Alexiou C, Bergemann C (2001) Clinical applications of magnetic drug targeting. J Surg Res 95:200-206
252. Lucca P, Hurrell R, Potrykus I (2001) Genetic engineering approaches to improve the bioavailability and the level of iron in rice grains. Theor Appl Genet 102:392-397
253. Machado MF, Saraiva JM (2005) Thermal stability and activity regain of horseradish peroxidase in aqueous mixtures of imidazolium-based ionic liquids. Biotechnol Lett 27:1233-1239
254. Maeda H, Chen L, Tsao G (1979) Glucoamylase immobilized on DEAE-cellulose beads in column raction. J Ferment Technol 57:238-243
255. Maksimenko AM (1998) New strategy of thrombolysis: Conjunctive effect of plasminogen activators with different pharmacokinetic profiles. Ann NY Acad Sci 864(1):96-105
256. Mandels M, Reese ET (1960) Induction of cellulases in fungi by cellobiose. J Bacteriol 79(6):816-826
257. Mandenius CF, Bülow L, Danielsson B et al. (1985) Monitoring and control of enzymic sucrose hydrolysis using on-line biosensors. Appl Microbiol Biotechnol 21(3-4):135-142
258. Marsden WL, Gray PP (1986) Enzymatic hydrolysis of cellulase in lignocellulosic materials. CRC Critic Rev Biotechnol 3:235-274
259. Martínez E, Hough G, Contarini A (1990) Sandiness prevention in dulce de leche by seeding with lactose microcrystals. J Dairy Sci 73:612-616
260. Marty A, ChulalaksananukulW,Willemot RM et al. (2004) Kinetics of lipase-catalyzed esterification in supercritical CO2. Biotechnol Bioeng 39:273-280
261. Marwaha SS, Kennedy JF (1988) Whey-pollution problems and potential utilization. Int J Food Sci Technol 23:323-336
262. Mason M (1983) Determination of glucose, sucrose, lactose, and ethanol in foods and beverages, using immobilized enzyme electrodes. J Assoc Off Anal Chem 66(4):981-984
263. Mateo C, Palomo JM, van Langen L et al. (2004) A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates. Biotechnol Bioeng 86:273-276
264. Mateo C, Abián A, Bernedo M et al. (2005) Some special features of glyoxyl supports to immobilize proteins. Enzyme Microb Technol 37:456:462
265. Mateo C, Palomo JM, Fernandez-Lorente G et al. (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol 40:1451-1463
266. Matsumoto K (1993) Removal of urea from alcoholic beverages by immobilized acid urease. In: In: Tanaka A, Tosa T, Kobayashi T (eds). Marcel Dekker, New York, pp 255-273
267. Maurer K (2004) Detergent proteases. Curr Opin Biotechnol 15:330-334
268. Melanson KJ, Angelopoulos TJ, Zukley LM et al. (2007) Effects of high-fructose corn syrup and sucrose consumption on circulating glucose, insulin, leptin, and ghrelin and on appetite in normal-weight women. Nutrition 23(2):103-112
269. Mesiano AJ, Beckman E, Russell AJ (1999) Supercritical biocatalysis. Chem Rev 99(2):623-633
270. Michael-Sinclair P (1965) Enzymes convert starch to dextrose. Chem Eng 72(18):90-96
271. Miller J, Nagarajan V (2000) The impact of biotechnology on the chemical industry in the 21st century. TIBTECH 18:190-196
272. Milosavić N, Prodanović R, Jovanović S et al. (2007) Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA). Enzyme Microb Technol 40(5):1422-1426
273. Mishra A, Debnath M (2002) Effect of pH on simultaneous saccharification and isomerization by glucoamylase and glucose isomerase. Appl Biochem Biotechnol 102-103:193-199
274. Mislovičová D, Masarová J, Bučo M et al. (2006) Stability of penicillin G acylase modified with various polysaccharides. Enzyme Microb Technol 39:579-585
275. Monte WC (1999) Ice cream containing a lactose enzyme: Composition and method. US Patent 5942264. Issued on August 24, 1999
276. Montenecourt BS, Eveleigh DE (1977) Preparation of mutants of Trichoderma reesei with enhanced cellulase production. Appl Environ Microbiol 34(6):777-782
277. Moore JC, Arnold FH (1996) Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents. Nat Biotechnol 14:458-467
278. Mori T, Kobayashi A, Okahata Y (1998) Biocatalytic esterification in supercritical carbon dioxide using a lipid-coated lipase. Chem Lett 9:921-922
279. Mori M, Gómez García R, Belleville MP (2005) A new way to conduct enzymatic synthesis in an active membrane using ionic liquids as catalyst support. Catal Today 104:313-317
280. Morley KL, Kazlauskas RJ (2005) Improving enzyme properties: When are closer mutations better? TIBTECH 23(5):231-237
281. Mozhaev VV, Khmelnitsky Y, Sergeeva MV et al. (1989) Catalytic activity and denaturation of enzymes in water/organic cosolvent mixtures. Alpha-chymotrypsin and laccase in mixed water/ alcohol, water/glycol and water/formamide solvents. Eur J Biochem 184:597-602
282. Nagasawa T, Yamada H (1990) Large-scale bioconversion of nitriles into useful amides and acids. In: Abramowicz DA (ed). Biocatalysis. Van Nostrand Reinhold, New York, pp 277-318
283. Nasser W, Chalet F, Robert-Baudouy J (1990) Purification and characterization of extracellular pectate lyase from Bacillus subtilis. Biochimie 72(9):689-695
284. Neidleman SL (1991) Historical perspective on the industrial uses of biocatalysts. In: Dordick JS (ed). Biocatalysts for industry. Plenum Press, New York, pp 21-33
285. Neuhaus W, Novalin S, Klimacek, M et al. (2006) Optimization of an innovative hollow-fiber process to produce lactose-reduced skim milk. Appl Biochem Biotechnol 134(1):1-14
286. Nield BS, Willows RD, Torda AE et al. (2002) New enzymes from environmental cassette arrays: Functional attributes of a phosphotransferase and an RNA-methyltransferase. Protein Sci 13:1651-1659
287. Nielsen PM, Olsen HS (2002) Enzymic modification of food protein. In: Whitehurst RJ, Law BA (eds). Enzymes in food technology. CRC Press, Boca Raton, pp 109-143
288. Nilsson H, Mosbach K, Enfors SO et al. (1978) An enzyme electrode for measurement of penicillin in fermentation broth: A step toward the application of enzyme electrodes in fermentation control. Biotechnol Bioeng 20(4):527-539
289. Noor R, Mittal S, Iqbal J (2002) Superoxide dismutase-Applications and relevance to human diseases. Med Sci Monit 8(9):210-215
290. Novalin S, Neuhaus W, Kulbe KD (2005) A new innovative process to produce lactose-reduced skim milk. J Biotechnol 119(2):212-218
291. O'Fágáin C (1997) Stabilizing protein function. Springer-Verlag, Berlin 200
292. O'Fágáin C (2003) Enzyme stabilization-recent experimental progress. Enzyme Microb Technol 33:137:149
293. Ogino H, Uchiho T, Yokoo J et al. (2001) Role of intermolecular disulfide bonds of the organic solvent stable PST-01 protease in its organic solvent stability. Appl Environ Microbiol 67:942-947
294. Okamoto S, Eltis LD (2007) Purification and characterization of a novel nitrile hydratase from Rhodococcus sp. RHA1. Molec Microbiol 65(3):828-838
295. O'Learys M, Brummund W (1974) pH jump studies of glutamate decarboxylase. Evidence for a pH-dependent conformation change. The J Biol Chem 249(12):3737-3745
296. Olsen HS (2002) Enzymes in starch modification. In: Whitehurst RJ, Law BA (eds). Enzymes in food technology. CRC Press, Boca Raton, pp 200-22
297. Olsen T, Kerton F, Marriott R et al. (2006) Biocatalytic esterification of lavandulol in supercritical carbon dioxide using acetic acid as the acyl donor. Enzyme Microb Technol 39(4):621-625
298. Ottolina G, de Gonzalo G, Carrea G et al. (2005) Enzymatic Baeyer-Villiger oxidation of bicyclic diketones. Adv Synth Catal 347(7-8):1035-104
299. Ouriel K, Veith FJ, Sasahara AA (1998) Comparison of recombinant urokinase with vascular surgery as initial treatment for acute arterial occlusion of the legs. New Eng J Med 338:1105-1111
300. Outtrup H, Boyce COL (1990) Microbial proteinases and biotechnology. In: Fogarty WM, Kelly CT (eds). Elsevier, London, New York, pp 133-176
301. Ovando-Chacón SL,Waliszeewski KN (2005) Preparativos de celulasas comerciales y aplicaciones en procesos extractivos. Universidad y Ciencia 21(42):113-122
302. Özturk DC, Kazan D, Erarslan A (2002) Stabilization and functional properties of Escherichia coli penicillin G acylase by covalent conjugation of anionic polysaccharide carboxymethyl cellulose. World J Microbiol Biotechnol 18:881-888
303. Padmakumar R, Oriel P (1999) Bioconversion of acrylonitrile to acrylamide using a thermostable nitrile hydratase. Appl Biochem Biotechnol 79(1-3):671-679
304. Parales RE, Lee K, Resnick SM et al. (2000) Substrate specificity of naphthalene dioxygenase: Effect of specific amino acids at the active site of the enzyme. J Bacteriol 182(6):1641-1649
305. Paramonov BA, Galenko-Yaroshevskii VP, Turkovskii II et al. (2005) Ointments with superoxide dismutase and interleukin-1β: Effect on reparative processes and impedance of burn wound. Bull Exp Biol Med 139(1):56-59
306. Park S, Kazlauskas RJ (2003) Biocatalysis in ionic liquids-Advantages beyond green. Curr Opin Biotechnol 14:432-437
307. Parmar A, Kumar H, Marwaha S et al. (2000) Advances in enzymatic transformation of penicillins to 6-Aminopenicillanic acid (6-APA). Biotechnol Adv 18:289-301
308. Pasamontes L, Haiker M, Wyss M et al. (1997) Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus. Appl Environ Microbiol 63(5):1696-1700
309. Pastores G, Thadhani R (2001) Enzyme-replacement therapy for Anderson-Fabry disease. Lancet 358(9282):601-603
310. Petkar M, Llai A, Caimi P et al. (2006) Immobilization of lipases for non-Aqueous synthesis. J Mol Catal B: Enzym 39:83-90
311. Petzelbauer I, Splechtna B, Nidetzky B (2002) Development of an ultrahigh-Temperature process for the enzymatic hydrolysis of lactose. III. Utilization of two thermostable β-glycosidases in a continuous ultrafiltration membrane reactor and galacto-oligosaccharide formation under steady state condition. Biotechnol Bioneg 77(4):394-404
312. Pingoud AM (2004) Restriction endonucleases. Springer, Berlin, Heidelberg, New York, 443
313. Polisson C (1992) Type II restriction endonuclease obtainable from Pseudomonas alcaligenes and a process for producing the same. US Patent 5098839. Publication date March 24, 1992
314. Pollard DJ, Woodley JM (2006) Biocatalysis for pharmaceutical intermediates: The future is now. TIBTECH 25(2):66-73
315. Poulsen PB (1984) Current application of immobilized enzymes for manufacturing purposes. Biotechnol Gen Eng Rev 1:121-139
316. Powell KA, Ramer SW, del Cardayré SB et al. (2001) Directed evolution and biocatalysis. Angew Chem Int Ed 40(21):3948-3959
317. Price CP (1983) Enzymes as reagents in clinical chemistry. Philos Trans R Soc Lond B Biol Sci 300 (1100) 411-422
318. Priolo N, Morcelle del Valle S, Arribere MC et al. (2000) Isolation and characterization of a cysteine protease from the latex of Araujia hortorum fruits. J Protein Chem 19(1):39-49
319. Quax W, Mrabet N, Litten R et al. (1991) Enhancing the thermostability of glucose isomerase by protein engineering. Bio/Technology 9:738-742
320. Quiroga E, Priolo N, Marchese J et al. (2005) Behaviour of araujiain, a cystein phytoprotease, in organic media with low water activity. Electron J Biotechnol 9:18-25
321. Quiroga E, Cami G, Marchese J et al. (2007) Organic solvents effect on the secondary structure of araujiain hI, in different media. Biochem Eng J 35:198-202
322. Qin Y, Cabral JMS (2002) Properties and applications of urease. Biocatal Biotransfor 20(1):1-4
323. Rachman B (1997) Unique features and application of non-Animal derived enzymes. Clin Nutr Ins 5(10):1-4
324. Rathi P, Bradoo S, Saxena RK et al. (2001) A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Proc Biochem 37:187-192
325. Reetz MT, Wiesenhöfer W (2004) Liquid poly(ethylene glycol) and supercritical carbon dioxide as a biphasic solvent system for lipase-catalyzed esterification. Chem Commun 2750-2751
326. Rehdorf J, Kirschner A, Bornscheuer UT (2007) Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440. Biotechnol Lett 29(9):1393-1398
327. Richter ER (1993) Biosensors: Applications for dairy food industry. J Dairy Sci 76(10):3114-3117
328. Roberts S (1998) Preparative biotransformations: The employment of enzymes and whole-cells in synthetic organic chemistry. J Chem Soc Perkin Trans 1:157-170
329. Roberts S (2000) Preparative biotransformations. J Chem Soc Perkin Trans 1:611-633
330. Roig M, Kennedy J (1992) Perspectives of chemical modification of enzymes. Critic Rev Biotechnol 12:391-412
331. Roy I, Gupta MN (2004) Hydrolysis of starch by a mixture of glucoamylase and pullulanase entrapped individually in calcium alginate beads. Enzyme Microb Technol 34(1):26-32
332. Roy JJ, Abraham TE (2006) Preparation and characterization of cross-linked enzyme crystals of laccase. J Mol Catal B: Enzym 38:31-36
333. Röhm O (1908) Preparation of hides for the manufacture of leather. US Patent 886411
334. Ru MT, Wu KC, Lindsay JP et al. (2002) Towards more active biocatalysts in organic media: Increasing the activity of salt-Activated enzymes. Biotechnol Bioeng 75(2):187-196
335. Russell AJ, Beckman E J (1991) Enzyme activity in supercritical fluids. Appl Biochem Biotechnol 31(2):197-202
336. Russell AJ, Beckman E J, Chaudhary AK (1994) Studying enzyme activity in supercritical fluids. Chemtech 24(3):33-37
337. Ryu DDY, Mandels M (1980) Cellulases: Biosynthesis and applications. Enzyme Microb Technol 2:91-102
338. Sabin RD,Wasserman BPJ (1987) A continuous spectrophotometric screening assay for glucoamylase Agric Food Chem 35:649-651
339. Salleh AB, Basri M, TaibM et al. (2002) Modified enzymes for reactions in organic solvents. Appl Biochem Biotechnol 102-103:349-357
340. San Martín R, Aguilera JM Hohlberg AI (1988) Effect of cellulose pretreatment on red-Algae agar extractability. Carbohyd Polym 8:33-43
341. Sarkissian CN, Shao Z, Blain F et al. (1999) A different approach to treatment of phenylketonuria: Phenylalanine degradation with recombinant phenylalanine ammonia lyase. Proc Natl Acad Sci USA 96(5):2339-2344
342. Sato T, Tosa T (1993a) Optical resolution of racemic amino acids by aminoacylase. In: Tanaka A, Tosa T, Kobayashi T (eds). Industrial application of immobilized biocatalysts. Marcel Dekker, New York, pp 1-14
343. Sato T, Tosa T (1993b) Production of L-Aspartic acid. In: Tanaka A, Tosa T, Kobayashi T (eds). Industrial application of immobilized biocatalysts. Marcel Dekker, New York, pp 15-24
344. Schaffeld G, Bruzzone P, Illanes A et al. (1989) Enzymatic treatment of stickwater from fishmeal industry with the protease from Cucurbita ficifolia. Biotechnol Lett 11(7):521-522
345. Schmedding DJM, van Gestel MJMC (2002) Enzymes in brewing. In: Whitehurst RJ, Law BA (eds). Enzymes in food technology. CRC Press, Boca Raton, USA, pp 57-75
346. Schmid A, Dordick JS, Hauer B et al. (2001) Industrial biocatalysis today and tomorrow. Nature 409:258-268
347. Schoemaker HE, Mink D, Wubbolts MG (2003) Dispelling the myths-biocatalysis in industrial synthesis. Science 299(5613):1694-1697
348. Schoevaart R, Wolbers M, Golubovic M et al. (2004) Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEA). Biotechnol Bioeng 87:754-762
349. Schubert C (2006) Can biofuels finally take center stage? Nat Biotechnol 24:777-784
350. Schumacher G, Sizmann D, Haug H et al. (1986) Penicillin acylase from E. coli: Unique gene-protein relation. Nucl Acid Res 14(14):5713-5727
351. Schügerl K (2001) Progress in monitoring, modeling and control of bioprocesses during the last 20 years. J Biotechnol 85(2):149-173
352. Schülein M (2000) Protein engineering of cellulases. Biochim Biophys Acta 1543:239-252
353. Schwedt G, Stein K (1994) Immobilized enzymes as tools in food analysis. Z Lebens Forsch 199(3):171-182
354. Scouten WH, Luong JHT, Brown RS (1995) Enzyme or protein immobilization techniques for application in biosensor design. TIBTECH 13:178-185
355. Scoville JR, Novicova IA (1999) Foaming enzyme spray cleaning composition and method of delivery. US Patent 5998342. Issued on December 7, 1999
356. Serafini S, Rossi L, Antonelli A et al. (2004) Drug delivery through phagocytosis of red blood cells. Transf Med Hemother 31:92-101
357. Shaw A, Bott R (1996) Engineering enzymes for stability. Curr Opin Struct Biol 6(4):546-550
358. Sheehan J, Himmel ME (1999). Enzymes, energy, and the environment: Cellulase development in the emerging bioethanol industry. Biotechnol Prog 15(3):817-827
359. Sheldon RA, Sorgedrager M, Janssen MHA (2007) Use of cross-linked enzyme aggregates (CLEAs) for performing biotransformations. Chem Today 25(1):62-67
360. Shibatani T, Nishimura N, Nabe K et al. (1974) Enzymatic production of urocanic acid by Achromobacter liquidum. Appl Microbiol 27(4):688-694
361. Shibuya H, Kaneko S, Hayashi K (2000) Enhancement of the thermostability and hydrolytic activity of xylanase by random gene shuffling. Biochem J 349:651-656
362. Si JQ, Drost-Lustenberger C (2002) Enzymes for bread, pasta and noodle products. In: Whitehurst RJ, Law BA (eds). Enzymes in food technology. CRC Press, Boca Raton, pp 19-56
363. Sierks M, Svennson B (1994) Protein engineering of the relative specificity of glucoamylase from Aspergillus awamori based on sequence similarities between starch degrading enzymes. Protein Eng 7:1479-1484
364. Soltys PJ, Mullon C, Langer R (1992) Oral treatment for jaundice using immobilized bilirubin oxidase. Artif Organs 16(4):331-335
365. Sonkaria S, Boucher G, Flórez-Alvarez J et al. (2004) Evidence for 'lock and key' character in an anti-phosphonate hydrolytic antibody catalytic site augmented by non-reaction centre recognition: Variation in substrate selectivity between an anti-phosphonate antibody, an anti-phosphate antibody and two hydrolytic enzymes. Biochem J 381:125-130
366. Sprössler B, Plainer H (1983) Immobilized lactase for processing whey. Food Technol 37:93-96
367. Stemmer WP (1994) Rapid evolution of a protein in vitro by DNA shuffling. Nature 370:389-391
368. Sticklen M (2006) Plant genetic engineering to improve biomass characteristics for biofuels. Curr Opin Biotechnol 17(3):315-319
369. Sugai T (1999) Applications of enzyme- and microorganism-catalyzed reactions to organic synthesis. Curr Org Chem 3:373-406
370. Sutherland TD, Horne I,Weir KM et al. (2004) Enzymatic bioremediation: From enzyme discovery to applications. Clin Exp Pharm Physiol 31(11)817-821
371. Stevenson D, Stanley R, Furneaux R (1993) Optimization of β-D-galactopyranoside synthesis from lactose using commercially available β-galactosidases. Biotechnol Bioeng 42:657-666
372. Svendsen A (2000) Lipase protein engineering. Biochim Biophys Acta 1543;223-238
373. Swi TM (2007) Artificial cells. World Scientific Publ, Singapore, 400
374. Sylvestre J, Chautard H, Cedrone F et al. (2006) Directed evolution of biocatalysts. Org Proc Res Dev 10(3):562-571
375. Takamine J (1894) Preparing and making taka-koji. US Patents 525820 and 525823
376. Tang XM, Lakay FM, ShenWet al. (2004) Purification and characterisation of an alkaline protease used in tannery industry from Bacillus licheniformis. Biotechnol Lett 26(18):1421-1424
377. Teplyakov AV, van der Laan JM, Lammers AA et al. (1992) Protein engineering of the highalkaline serine protease PB92 from Bacillus alcalophilus: Functional and structural consequences of mutation at the S4 substrate binding pocket. Prot Eng 5(5):413-420
378. Terao Y, Miyamoto K, Ohta H (2006) Introduction of single mutation changes arylmalonate decarboxylase to racemase. Chem Commun 3600-3602
379. Thanikaivelan P, Rao JR, Nair BU et al. (2004) Progress and recent trends in biotechnological methods for leather processing. TIBTECH 22(4)181-188
380. Thayer AM (2006) Enzymes at work. Chem Eng News 84(339:15-25
381. Thomas SM, DiCosimo R, Nagarajan V (2002) Biocatalysis: Applications and potentials for the chemical industry. TIBTECH 20(6):238-242
382. Tindall KR, Kunkel TA (1988) Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Biochemistry 27:6008-6013
383. Tjalsma H, Koetje EJ, Kiewiet R et al. (2004) Engineering of quorum-sensing systems for improved production of alkaline protease by Bacillus subtilis. J Appl Microbiol 96(3):569-578
384. Torres S, Castro G (2004) Non-Aqueous biocatalysis in homogeneous solvent systems. Food Technol Biotechnol 42(4):271-277
385. Tramper J (1994) Applied biocatalysis: From product request to idea to product. In: Cabral JMS, Boros DBL, Tramper J (eds). Applied biocatalysis. Harwood Acad Publ, Chur, pp 1-45
386. Trivedi AH, Spiess AC, Daussmann T et al. (2006) Effect of additives on gas-phase catalysis with immobilised Thermoanaerobacter species alcohol dehydrogenase. Appl Microbiol Biotechnol 71(4):407-414
387. Trojanowicz M (2006) Analytical applications of carbon nanotubes: A review. Trends Anal Chem 25(5):480-489
388. Tryfona T, Bustard MT (2005) Fermentative production of lysine by Corynebacterium glutamicum: Transmembrane transport and metabolic flux analysis. Proc Biochem 40(2):499-508
389. Trzecieski J (1983) Lactose-reduced ice cream and process for the production thereof. US Patent 4374861
390. Tucker GA (1995) Fundamentals of enzyme activity. In: Tucker GA, Woods LFJ (eds). Enzymes in food processing. Blackie Academic & Professional, London, pp 1-26
391. Uhlig H (1998) Industrial enzymes and their applications. Wiley-IEEE, New York, 454
392. Ulbert O, Belafi-Bakó K, Tonova K et al. (2005) Thermal stability of Candida rugosa lipase using ionic liquids. Biocatal Biotransfor 23(3-4):177-183
393. Ulijn RV, Janssen AEM, Moore BD et al. (2001) Predicting when precipitation-driven synthesis is feasible: Applications to biocatalysis. Chem Eur J 7(10):2089-2097
394. Ulijn RV, De Martin L, Gardossi L et al. (2002a) Solvent selection for solid-To-solid synthesis. Biotechnol Bioeng 80(5):509-515
395. Ulijn RV, De Martin L, Hallinga PJ et al. (2002b) Enzymatic synthesis of β-lactam antibiotics via direct condensation. J Biotechnol 99(3):215-222
396. Ulijn RV, Martin LD, Gardossi L et al. (2003) Biocatalysis in reaction mixtures with undissolved solid substrates and products. Curr Org Chem 7(13):1333-1346
397. Undurraga D, Markovits A, Erazo S (2001) Cocoa butter equivalent through enzymic interesterification of palm oil midfraction. Proc Biochem 36:933-939
398. van Beilen JB, Li Z (2002) Enzyme technology: An overview. Curr Opin Biotechnol 13:338-344
399. van Brunt J (1987) Biosensors for bioprocesses. Biotechnology 5(5):437-440
400. van den Burg B, Eijsink VGH (2002) Selection of mutations for increased protein stability. Curr Opin Biotechnol 13:333-337
401. van den Burg B (2003) Extremophiles as a source for novel enzymes. Curr Opin Microbiol 6:213-218
402. van den Hout H, Reuser A, Vulto A et al. (2000) Recombinant human α-glucosidase from rabbit milk in Pompe patients. Lancet 356(9227):397-398
403. van der Donk WA, Zhao H (2003) Recent developments in pyridine nucleotide regeneration. Curr Opin Biotechnol 14:421-426
404. van der Werf MJ, van den Tweel WJJ, Kamphuis J et al. (1994) The potential of lyases for the industrial production of optically active compounds. TIBTECH 12:95-103
405. van Dijk PWM (1999) Chymosyn and phytase made by genetic engineering. J Biotechnol 67:77-80
406. van Griethuysen-Dilber E, Flaschel E, Renken A (1988) Process development for the hydrolysis of lactose in whey by immobilized Aspergillus oryzae. Proc Biochem 23(4):55-59
407. van Langen LM, de Vrooom E, van Rantwijk F et al. (1999) Enzymatic synthesis of β-lactam antibiotics using penicillin G acylase in frozen media. FEBS Lett 4566:89-92
408. van Langen LM, van Rantwijk F, Svedas VK et al. (2000) Penicillin acylase catalyzed peptide synthesis: A chemoenzymatic route to stereoisomers of 3,6diphenylpiperazine 2,5 dione. Tetrahed Assym 11:1077-1083
409. van Oort M, Canal-Llaubères RM (2002) Enzymes in wine production In: Whitehurst RJ, Law BA (eds). Enzymes in food technology. CRC Press, Boca Raton, pp 76-108
410. van Rantwijk F, Lau RM, Sheldon RA (2003) Biocatalytic transformations in ionic liquids. TIBTECH 21(3):131-138
411. van Unen DJ, Engbersen FJ, Reinhoudt DN (2001) Studies on the mechanism of crown-etherinduced activation of enzymes in non-Aqueous media. Biotechnol Bioeng 75:154-158
412. Varela H, Ferrari MD, Belobradjic L et al. (1997) Skin unhairing proteases of Bacillus subtilis: Production and partial characterization. Biotechnol Lett 19:755-758
413. Verduyn C, Zomerdijk TPL, van Dijken JHP et al. (1984) Continuous measurement of ethanol production by aerobic yeast suspensions with an enzyme electrode. J Appl Microbiol Biotechnol 19(3):181-185
414. Verma IM, Somia N (1997) Gene therapy-promises, problems and prospects. Nature 389:239-242
415. Verma N, Kumar K, Kaur G et al. (2007) L-Asparaginase: A promising chemotherapeutic agent. Crit Rev Biotechnol 27:45-62
416. Vidal L, Ferrer P, Alvaro G et al. (2005) Influence of induction and operation mode on recombinant rhamnulose-1-phosphate aldolase production in Escherichia coli using the T5 promoter. J Biotechnol 118:75-87
417. Vohra A, Satyanarayana T (2003) Phytases: Microbial sources, production, purification and potential biotechnological applications. Critic Rev Biotechnol 23(1):29-60
418. Walsh GA, Powerr RF, Headon DR (1993) Enzymes in the animal-feed industry. TIBTECH 11:424-430
419. Wandrey C, Liese A, Kihumbu D (2000) Industrial biocatalysis: Past, present, and future. Org Proc Res Develop 4(4):286-290
420. Wegman M, Janssen M, van Rantwijk F et al. (2001) Towards biocatalytic synthesis of β-lactam antibiotics. Adv Synth Catal 343:559-576
421. Weigel B, Hitzmann B, Kretzmer G et al. (1996) Analysis of various sugars by means of immobilized enzyme coupled flow injection analysis. J Biotechnol 50(2-3):93-106
422. Weissensteiner T, Griffin AM, Griffin HG (2003) PCR technology: Current innovations. CRC Press, Boca Raton, 416
423. Wescott CR, Klibanov AM (1994) The solvent dependence of enzyme specificity. Biochim Biophys Acta 1206:1-9
424. Whitaker JR (1990) Microbial pectolytic enzyme. In: Fogarty WM, Kelly CT (eds). Elsevier, London, New York, pp 133-176
425. Whitehurst RJ, Law BA (2002) Enzymes in food technology. CRC Press, Boca Raton, USA, 255 pp. ISBN 0849397820
426. Wilson L, Illanes A, Pessela CB et al. (2004a) Encapsulation of crosslinked penicillin G acylase aggregates in Lentikats: Evaluation of a novel biocatalyst in organic media. Biotechnol Bioeng 86(5):558-562
427. Wilson L, Illanes A, Abián O et al. (2004b) Co-Aggregation of penicillin G acylase and polyionic polymers: An easy methodology to prepare enzyme biocatalysts stable in organic media. Biomacromolecules 5:852-857
428. Wilson L, Fernández-Lorente G, Fernández-Lafuente R et al. (2006). CLEAs of lipases and polyionic polymers: A simple way of preparing stable biocatalysts with improved properties. Enzyme Microb Technol 39:750-755
429. Winkler ME, Blaber M, Bennett GL et al. (1985) Purification and characterization of recombinant urokinase from Escherichia coli. Bio/Technology 3:990-1000
430. Wisdom GB (1976) Enzyme-immunoassay. Clin Chem 22:1243-1255
431. Wong DWS (1995) Food enzymes: Structure and mechanisms. Chapman Hall, New York, 390
432. Woodyer R, van der Donk WA, Zhao H (2006) Optimizing a biocatalyst for improved NAD(P)H regeneration: Directed evolution of phosphite dehydrogenase. Comb Chem 9:237-245
433. Wortmann RL (2005) Recent advances in the management of gout and hyperuricemia. Curr Opin Rheumat 17(3):319-324
434. Wrotnowsky C (1997) Unexpected niche applications for industrial enzymes drives market growth. Gen Eng News 17(3):14-30
435. Wyman CE (1996) Bioethanol: Production and utilization. Taylor & Francis, Bristol, PA, USA, 424 pp. ISBN 1560325534
436. Xu F, Ding H (2007) A new kinetic model for heterogeneous (or spatially confined) enzymatic catalysis: Contributions from the fractal and jamming (overcrowding) effect. Appl Catal A: General 317(1):70-81
437. Xu Q, Zhu JJ, Hu XY (2007) Ordered mesoporous polyaniline film as a new matrix for enzyme immobilization and biosensor construction. Anal Chim Acta 597(1):151-156
438. Yakovleva J, Davidsson R, Lobanova A et al. (2002) Enzyme immunoassay using silicon microchip with immobilized antibodies and chemiluminescence detection. Anal Chem 74(13):2994-3004
439. Yamada H, Kobayashi M (1996) Nitrile hydratase and its application to industrial production of acrylamide. Biosci Biotechnol Biochem 60:1391-1400
440. Yamamoto K, Ohgaru T, Torimura M et al. (2000) Highly-sensitive flow injection determination of hydrogen peroxide with a peroxidase-immobilized electrode and its application to clinical chemistry. Anal Chim Acta 40(2):201-207
441. Yan AX, Li XW, Ye YH (2002) Recent progress on immobilization of enzymes in molecular sieves for reactions in organic solvents. Appl Biochem Biotechnol 101:113-129
442. Yang VC, Bernstein H, Cooney CL et al. (1986) Removal of the anticoagulant activities of the low molecular weight heparin fractions and fragments with flavobacterial heparinase. Thromb Res 44(5):599-610
443. Yousef MS, Clark SA, Pruett PK et al. (2003) Induced fit in guanidino kinases-comparison of substrate-free and transition state analog structures of arginine kinase. Protein Sci 12:103-111
444. Youshko MI, Švedas VK (2002) Penicillin acylase-catalyzed solid state ampicillin synthesis. Adv Sinth Catal 344:894-898
445. Zaks A, Klibanov AM (1984) Enzymatic catalysis in organic media at 100°C. Science 224:1249-1251
446. Zaks A, Klibanov AM(1985) Enzyme-catalyzed processes in organic solvents. Proc Natl Acad Sci USA 82:3192-3196
447. Zaks A, Klibanov AM (1988a) Enzymatic catalysis in nonaqueous solvents. J Biol Chem 263:3194-3201
448. Zaks A, Klibanov AM (1988b) The effect of water on enzyme action in organic media. J Biol Chem 263:8017-8021
449. Zeikus JG, Vieille C, Savchenko A (2004) Thermozymes: Biotechnology and structure-function relationships. Extremophiles 2(3):179-183
450. Zhao H, Giver L, Shao Z et al. (1998) Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat Biotechnol 16:258-261
451. Zhao H, Chockalingam K, Chen Z (2002) Directed evolution of enzymes and pathways for industrial biocatalysis. Curr Opin Biotechnol 13(2):104-10