Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus

Applied and Environmental Microbiology

Volumn 63, Issue 5, 1997, Pages 1696-1700

  Pasamontes, Luis ^a   Haiker, Monika ^a   Wyss, Markus ^a   Tessier, Michel ^a   Van Loon, Adolphus P G M ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; FUNGAL PROTEIN; PHYTASE; PHYTIC ACID;

ARTICLE; ASPERGILLUS FUMIGATUS; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME PURIFICATION; FUNGUS; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS; TEMPERATURE SENSITIVITY;

EID: 0030898612     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.5.1696-1700.1997     Document Type: Article

References (21)

1. Breathnach, R., C. Benoist, K. O'Hare, F. Gannon, and P. Chambon. 1978. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc. Natl. Acad. Sci. USA 75:4853-4857.
2. Cromwell, G. L., and R. D. Coffey. 1991. Phosphorus, a key essential nutrient, yet a possible major pollutant - its central role in animal nutrition, p. 134-145. In T. P. Lyons (ed.), Biotechnology in the feed industry. Alltech Technical Publications, Nicholasville, Ky.
3. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
4. Don, R. H., P.T. Cox, B. J. Wainwright, K. Baker, and J. S. Mattick. 1991. "Touchdown" PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 19:4008.
5. Dox, A. W., and R. Golden. 1911. Phytase in lower fungi. J. Biol. Chem. 10: 183-185.
6. Gibson, K. 1995. The pelleting stability of animal feed enzymes, p. 157-162. In W. Van Hartingsveldt, M. Hessing, J. P. van der Lugt, and W. A. C. Somers (ed.), Proceedings of the Second European Symposium on Feed Enzymes. TNO Nutrition and Food Research Institute, Zeist, The Netherlands.
7. Heery, D. M., F. Gannon, and R. Powell. 1990. A simple method for subclonins DNA fragments from gel slices. Trends Genet. 6:173.
8. Lloyd, A. T., and P. M. Sharp. 1991. Codon usage in Aspergillus nidulans. Mol. Gen. Genet. 230:288-294.
9. Lolas, G. M., and P. Markakis. 1977. Phytase of navy beans. J. Food Sci. 42:1094-1097.
10. Mitchell, D. B., K. Vogel, B. J. Weimann, L. Pasamontes, and A. P. G. M. van Loon. 1997. The phytase subfamily of histidinc acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila. Microbiology 143:245-252.
11. Mozhaev, V. V. 1993. Review: mechanism-based strategies for protein thermostabilization. Trends Biotechnol. 11:88-95.
12. Mullaney, E. J., J. E. Hamer, K. A. Roberti, M. M. Yelton, and W. E. Timberlake. 1985. Primary structure of the trpC gene from Aspergillus nidulans. Mol. Gen. Genet. 199:37-45.
13. Nelson, T. S., T. R. Shieh, R. J. Wodzinsky, and J. H. Ware. 1971. Effect of supplemental phytase on the utilization of phytate phosphorus by chicks. J. Nutr. 101:1289-1294.
14. Piddington, C. S., C. S. Houston, M. Paloheimo, M. Cantrell, A. Miettinen-Oinonen, H. Nevalainen, and J. Rambosek. 1993. The cloning and sequencing of the genes encoding phytase (phy) and pH2.5-optimum acid phosphatase (aph) from Aspergillus niger var. awamori. Gene 133:55-62.
15. Punt, P. J., and C. A. van den Hondel. 1992. Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers. Methods Enzymol. 216:447-457.
16. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
17. Ullah, A. H. J. 1988. Aspergillus ficuum phytase: partial primary structure, substrate selectivity, and kinetic characterization. Prep. Biochem. 18:459-471.
18. Van den Hondel, C. A. M. J. J., P. J. Punt, and R. F. M. van Gorcom. 1991. Heterologous gene expression in filamentous fungi, p. 396-428. In J. W. Bennett and L. L. Lasure (ed.), More gene manipulations in fungi. Academic Press, Inc., San Diego, Calif.
19. Van Hartingsveldt, W., C. M. J. Van Zeijl, G. M. Harteveld, R. J. Gouka, M. E. G. Suykerbuyk, R. G. M. Luiten, P. A. Van Paridon, G. C. M. Selten, A. E. Veenstra, R. F. van Gorcom, and C. A. van den Hondel. 1993. Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger. Gene 127:87-94.
20. Vincent, J. B., M. W. Crowder, and B. A. Averill. 1992. Hydrolysis of phosphate monoesters: a biological problem with multiple chemical solutions. Trends Biochem. Sci. 17:105-110.
21. von Heijne, G. 1983. Patterns of amino acids near signal sequence cleavage sites. Eur. J. Biochem. 133:17-21.