Directed Evolution of a Para-Nitrobenzyl Esterase for Aqueous-Organic Solvents

Nature Biotechnology

Volumn 14, Issue 4, 1996, Pages 458-467

  Moore, Jeffrey C ^a   Arnold, Frances H ^a  

^a California Institute of Technology ^*  (United States)

Author keywords

Antibiotic synthesis; Enzymatic deprotection; Random mutagenesis

Indexed keywords

ANTIBIOTIC AGENT; ESTERASE; LORACARBEF; ORGANIC SOLVENT;

ARTICLE; CATALYSIS; DRUG STRUCTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; MUTAGENESIS; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

AMINO ACID SUBSTITUTION; ANTI-BACTERIAL AGENTS; BACILLUS SUBTILIS; BASE SEQUENCE; BINDING SITES; BIOTECHNOLOGY; CARBOXYLIC ESTER HYDROLASES; DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; DNA, RECOMBINANT; ESCHERICHIA COLI; GENES, BACTERIAL; KINETICS; MODELS, MOLECULAR; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SOLVENTS; WATER;

EID: 0029670577     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt0496-458     Document Type: Article

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