Preparation, optimization, and structures, of cross-linked enzyme aggregates (CLEAs)

Biotechnology and Bioengineering

Volumn 87, Issue 6, 2004, Pages 754-762

  Schoevaart, R ^a,b   Wolbers, M W ^a   Golubovic, M ^a   Ottens, M ^a   Kieboom, A P G ^b   Van Rantwijk, F ^a   Van Der Wielen, L A M ^a   Sheldon, R A ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^b LEIDEN UNIVERSITY   (Netherlands)

Author keywords

CLEA; CLEC; Immobilization; Protein

Indexed keywords

AGGLOMERATION; BIOTECHNOLOGY; CROSSLINKING; ENZYME IMMOBILIZATION; ENZYME KINETICS; OPTIMIZATION;

AGGREGATION METHODS; CROSS-LINKED ENZYME AGGREGATES (CLEA);

ENZYMES;

ARTICLE; CROSS LINKING; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME ISOLATION; ENZYME STRUCTURE; HIGH THROUGHPUT SCREENING; PROTEIN AGGREGATION; TECHNIQUE;

CROSS-LINKING REAGENTS; ENZYME ACTIVATION; ENZYMES; ENZYMES, IMMOBILIZED; FRACTIONAL PRECIPITATION; MULTIPROTEIN COMPLEXES; PARTICLE SIZE; PROTEIN CONFORMATION;

EID: 4644232857     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20184     Document Type: Article

References (23)

1. Asaad N, Engberts JBFN. 2003. Cytosol-mimetic chemistry: Kinetics of the trypsin-catalyzed hydrolysis of p-nitrophenyl acetate upon addition of polyethylene glycol and N-tert-butyl acetoacetamide. J Am Chem Soc 125:6874-6875.
2. Avigad G, Asensio C, Horecker BL, Amaral D. 1962. D-Galactose oxidase of Polyporys circinatus. J Biol Chem 237:2736-2740.
3. Boller T, Meier C, Menzler S. 2002. Eupergit oxirane acrylic beads: How to make the enzyme fit for biocatalysts. Org Proc Res Dev 6:509-519.
4. Bourbonnais R, Paice MG. 1990. Oxidation of nonphenolic substrates-An expanded role for laccase in lignin biodegradation. FEBS Lett 267:99-102.
5. Cao LQ, van Rantwijk F, Sheldon RA. 2000. Cross-linked enzyme aggregates: A simple and effective method for the immobilization of Penicillin acylase. Org Lett 2:1361-1364.
6. Cao LQ, van Langen LM, van Rantwijk F, Sheldon RA. 2001. Cross-linked aggregates of Penicillin acylase: Robust catalysts for the synthesis of β-lactam antibiotics. J Mol Cat B:Enzym 11:665-670.
7. Cao LQ, van Langen LM, Sheldon RA. 2003. Immobilised enzymes: Carrier-bound or carrier-free? Curr Opin Biotechnol 14(4):387-394.
8. Doscher MS, Richards FM. 1963. The actvitiy of an enzyme in the crystalline state: Ribonuclease S. J Biol Chem 238:2399-2406.
9. Dvorakova J, Volfova O, Kopecky J. 1997. Characterization of phytase produced by Aspergillus niger. Folia Microbiologica 42:349-352.
10. Gröger H, Hummel W, Rollmann C, Chamouleau F, Hüsken H, Werner H, Wunderlich C, Abokitse K, Drauz K, Buchholza S. 2004. Preparative asymmetric reduction of ketones in a biphasic medium with an (S)-alcohol dehydrogenase under in situ-cofactor-recycling with a formate dehydrogenase. Tetrahedron 60:633-640.
11. Häring D, Schreier P. 1999. Cross-linked enzyme crystals. Curr Opin Biotechnol 3:35-38.
12. Hofland GW, de Rijke A, Thiering R, van der Wielen LAM, Witkamp GJ. 2000. Isoelectric precipitation of soybean protein using carbon dioxide as a volatile acid. J Chromatogr B 743:357-368.
13. Kim CS, Ji E-S, Oh D-K. 2003. Expression and characterization of Kluyveromyces lactis β-galactosidase in Escherichia coli. Biotechnol Lett 25:1769-1774.
14. Kragl U. 1996. Enzyme membrane reactors. In: Godfrey T, West S, editors. Industrial enzymology-Application of enzymes in industry, 2nd ed. London: MacMillan. p 271-283.
15. Lalonde J. 1997. Practical catalysis with enzyme crystals. Chemtech 27 (2):38-45.
16. López-Serrano P, Cao L, van Rantwijk F, Sheldon RA. 2002. Cross-linked enzyme aggregates with enhanced activity: Application to lipases. Biotechnol Lett 24:1379-1383.
17. Margolin AL. 1996. Novel crystalline catalysts. Trends Biotechnol 14: 223-230.
18. Margolin AL, Navia MA. 2001. Protein crystals as novel catalytic materials. Angew Chem Int Ed Engl 40:2204-2222.
19. Messing RA. 1975. Immobilized enzymes for industrial reactors. London: Academic Press.
20. Quiocho FA, Richards FM. 1964. Intermolecular cross-linking of a protein in the crystalline state: Carboxypeptidase A. Proc Natl Acad Sci USA 52:833-839.
21. Rella R, Raia CA, Pensa M, Pisani FM, Gambacorta A, De Rosa M, Rossi M. 1987. A novel archaebacterial NAD+-dependent alcohol dehydrogenase: Purification and properties. Eur J Biochem 167:475-479.
22. St. Clair NL, Navia MA. 1992. Cross-linked enzyme crystals as robust biocatalysts. J Am Chem Soc 114:7314-7316.
23. Van Langen LM, Janssen MHA, Oosthoek NHP, Pereira SRM, Svedas VK, van Rantwijk F, Sheldon RA. 2002. Active site titration as a tool for the evaluation of immobilization procedures of Penicillin acylase. Biotechnol Bioeng 79(2):224-228.