-
1
-
-
0034667190
-
High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: Statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2
-
Abecassis, V., Pompon, D., and Truan, G. 2000. High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2. Nucleic Acids Res. 28: e88-98.
-
(2000)
Nucleic Acids Res
, vol.28
-
-
Abecassis, V.1
Pompon, D.2
Truan, G.3
-
2
-
-
33750026145
-
Producing chimeric genes by CLERY: In vitro and in vivo recombination
-
Abecassis, V., Pompon, D., and Truan, G. 2003. Producing chimeric genes by CLERY: in vitro and in vivo recombination. Methods Mol Biol. 231: 165-173.
-
(2003)
Methods Mol Biol
, vol.231
, pp. 165-173
-
-
Abecassis, V.1
Pompon, D.2
Truan, G.3
-
3
-
-
4143110558
-
Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase
-
Acharya, P., Rajakumara, E., Sankaranarayanan, R., and Rao, N. M. 2004. Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase. J Mol Biol. 341(5): 1271-1281.
-
(2004)
J Mol Biol
, vol.341
, Issue.5
, pp. 1271-1281
-
-
Acharya, P.1
Rajakumara, E.2
Sankaranarayanan, R.3
Rao, N.M.4
-
4
-
-
0034628501
-
Directed evolution of new catalytic activity using the α/β-barrel scaffold
-
Altamirano, M. M., Blackburn, J. M., Aguayo, C., and Fersht, A. R. 2000. Directed evolution of new catalytic activity using the α/β-barrel scaffold. Nature. 403: 617-622.
-
(2000)
Nature
, vol.403
, pp. 617-622
-
-
Altamirano, M.M.1
Blackburn, J.M.2
Aguayo, C.3
Fersht, A.R.4
-
5
-
-
0033023419
-
Directed evolution of biocatalysts
-
Arnold, F. H., and Volkov, A. A. 1999. Directed evolution of biocatalysts. Curr Opin Chem Biol. 3(1): 54-59.
-
(1999)
Curr Opin Chem Biol
, vol.3
, Issue.1
, pp. 54-59
-
-
Arnold, F.H.1
Volkov, A.A.2
-
6
-
-
18044384249
-
Alteration of substrate specificity of aspartase by directed evolution
-
Asano, Y., Kira, I., and Yokozeki, K. 2005. Alteration of substrate specificity of aspartase by directed evolution. Biomol Eng. 22(1-3): 95-101.
-
(2005)
Biomol Eng
, vol.22
, Issue.1-3
, pp. 95-101
-
-
Asano, Y.1
Kira, I.2
Yokozeki, K.3
-
7
-
-
18044383127
-
Engineering the substrate specificity of cytochrome P450 CYP102A2 by directed evolution: Production of an efficient enzyme for bioconversion of fine chemicals
-
Axarli, I., Prigipaki, A., and Labrou, N. E. 2005. Engineering the substrate specificity of cytochrome P450 CYP102A2 by directed evolution: production of an efficient enzyme for bioconversion of fine chemicals. Biomol Eng. 22(1-3): 81-88.
-
(2005)
Biomol Eng
, vol.22
, Issue.1-3
, pp. 81-88
-
-
Axarli, I.1
Prigipaki, A.2
Labrou, N.E.3
-
8
-
-
16244422032
-
On the origin of genomic adaptation at high temperature for prokaryotic organisms
-
Basak, S., and Ghosh, T. C. 2005. On the origin of genomic adaptation at high temperature for prokaryotic organisms. Biochem Biophys Res Commun. 330(3): 629-632.
-
(2005)
Biochem Biophys Res Commun
, vol.330
, Issue.3
, pp. 629-632
-
-
Basak, S.1
Ghosh, T.C.2
-
9
-
-
21244478636
-
Engineering of pyranose 2-oxidase from Peniophora gigantea towards improved thermostability and catalytic efficiency
-
Bastian, S., Rekowski, M. J., Witte, K., Heckmann-Pohl, D. M. and Giffhorn, F. 2005. Engineering of pyranose 2-oxidase from Peniophora gigantea towards improved thermostability and catalytic efficiency. Appl Microbiol Biotechnol. 67(5): 654-663.
-
(2005)
Appl Microbiol Biotechnol
, vol.67
, Issue.5
, pp. 654-663
-
-
Bastian, S.1
Rekowski, M.J.2
Witte, K.3
Heckmann-Pohl, D.M.4
Giffhorn, F.5
-
10
-
-
11844273937
-
Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery
-
Bernath, K., Magdassi, S., and Tawfik, D. S. 2005. Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery. J Mol Biol. 345(5): 1015-1026.
-
(2005)
J Mol Biol
, vol.345
, Issue.5
, pp. 1015-1026
-
-
Bernath, K.1
Magdassi, S.2
Tawfik, D.S.3
-
11
-
-
23044501802
-
Evolving strategies for enzyme engineering
-
Bloom, J. D., Meyer, M. M., Meinhold, P., Otey, C. R., Macmillan, D., and Arnold, F. H. 2005. Evolving strategies for enzyme engineering. Curr Opin Struct Biol. 15(4): 447-452.
-
(2005)
Curr Opin Struct Biol
, vol.15
, Issue.4
, pp. 447-452
-
-
Bloom, J.D.1
Meyer, M.M.2
Meinhold, P.3
Otey, C.R.4
Macmillan, D.5
Arnold, F.H.6
-
13
-
-
0036300748
-
Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene
-
Bosma, T., Damborsky, J., Stucki, G., and Janssen, D. B. 2002. Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene. Appl Environ Microbiol. 68(7): 3582-3587.
-
(2002)
Appl Environ Microbiol
, vol.68
, Issue.7
, pp. 3582-3587
-
-
Bosma, T.1
Damborsky, J.2
Stucki, G.3
Janssen, D.B.4
-
14
-
-
0003534466
-
-
VCH, Weinheim, Germany
-
Brunner, H., and Zettllmeier, W. 1993. Handbook of Enantioselective Catalysis with Transition Metal Compounds, vol I-II. VCH, Weinheim, Germany.
-
(1993)
Handbook of Enantioselective Catalysis with Transition Metal Compounds
, vol.1-2
-
-
Brunner, H.1
Zettllmeier, W.2
-
15
-
-
85045502002
-
Randomization of genes by PCR mutagenesis
-
Cadwell, R. C., and Joyce, G. F. 1992. Randomization of genes by PCR mutagenesis. PCR Methods Appl. 2(1): 28-33.
-
(1992)
PCR Methods Appl
, vol.2
, Issue.1
, pp. 28-33
-
-
Cadwell, R.C.1
Joyce, G.F.2
-
16
-
-
0003134846
-
Mutagenic PCR
-
Dieffenbach, C.W. and Dveksler, G.S., Eds. CSHL Press, New York
-
Cadwell, R. C., and Joyce, G. J. 1995. Mutagenic PCR. In: PCR Primer: A Laboratory Manual. pp. 583-9. Dieffenbach, C.W. and Dveksler, G.S., Eds. CSHL Press, New York.
-
(1995)
PCR Primer: A Laboratory Manual
, pp. 583-589
-
-
Cadwell, R.C.1
Joyce, G.J.2
-
17
-
-
0036136382
-
Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation
-
Canada, K. A., Iwashita, S., Shim, H., and Wood, T. K. 2002. Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation. J. Bacteriol. 184: 344-349.
-
(2002)
J Bacteriol
, vol.184
, pp. 344-349
-
-
Canada, K.A.1
Iwashita, S.2
Shim, H.3
Wood, T.K.4
-
18
-
-
0035177610
-
Enzyme engineering: Rational redesign versus directed evolution
-
Chen, R. 2001. Enzyme engineering: rational redesign versus directed evolution. Trends Biotechnol. 19(1): 13-14.
-
(2001)
Trends Biotechnol
, vol.19
, Issue.1
, pp. 13-14
-
-
Chen, R.1
-
19
-
-
0036208056
-
Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents
-
Cho, C. M.-H., Mulchandani, A., and Chen, W. 2002. Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents. Appl Environ Microbiol. 68(4): 2026-2030.
-
(2002)
Appl Environ Microbiol
, vol.68
, Issue.4
, pp. 2026-2030
-
-
Cho, C.M.-H.1
Mulchandani, A.2
Chen, W.3
-
20
-
-
4143066086
-
Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos
-
Cho, C. M.-H., Mulchandani, A., and Chen, W. 2004. Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos. Appl Environ Microbiol. 70(8): 4681-4685.
-
(2004)
Appl Environ Microbiol
, vol.70
, Issue.8
, pp. 4681-4685
-
-
Cho, C.M.-H.1
Mulchandani, A.2
Chen, W.3
-
21
-
-
32844460390
-
Directed evolution of ATP binding proteins from a zinc finger domain by using mRNA display
-
Cho, G. S., and Szostak, J. W. 2006. Directed evolution of ATP binding proteins from a zinc finger domain by using mRNA display. Chem Biol. 13(2): 139-147.
-
(2006)
Chem Biol
, vol.13
, Issue.2
, pp. 139-147
-
-
Cho, G.S.1
Szostak, J.W.2
-
22
-
-
4944240750
-
Narrowing substrate specificity in a directly evolved enzyme: The A293D mutant of aspartate aminotransferase
-
Chow, M. A., McElroy, K. E., Corbett, K. D., Berger, J. M., and Kirsch, J. F. 2004. Narrowing substrate specificity in a directly evolved enzyme: The A293D mutant of aspartate aminotransferase. Biochemistry. 43(40): 12780-12787.
-
(2004)
Biochemistry
, vol.43
, Issue.40
, pp. 12780-12787
-
-
Chow, M.A.1
McElroy, K.E.2
Corbett, K.D.3
Berger, J.M.4
Kirsch, J.F.5
-
23
-
-
0042522511
-
Global incorporation of norleucine in place of methionine in cytochrome P450 BM-3 heme domain increases peroxygenase activity
-
Cirino, P. C., Tang, Y., Takahashi, K., Tirrell, D. A., and Arnold, F. H. 2003. Global incorporation of norleucine in place of methionine in cytochrome P450 BM-3 heme domain increases peroxygenase activity. Biotechnol Bioeng. 83(6): 729-734.
-
(2003)
Biotechnol Bioeng
, vol.83
, Issue.6
, pp. 729-734
-
-
Cirino, P.C.1
Tang, Y.2
Takahashi, K.3
Tirrell, D.A.4
Arnold, F.H.5
-
24
-
-
9644266716
-
Alcohol-mediated error-prone PCR
-
Claveau, S., Sasseville, M., and Beauregard, M. 2004. Alcohol-mediated error-prone PCR. DNA Cell Biol. 23(11): 789-795.
-
(2004)
DNA Cell Biol
, vol.23
, Issue.11
, pp. 789-795
-
-
Claveau, S.1
Sasseville, M.2
Beauregard, M.3
-
25
-
-
0035067250
-
DNA shuffling method for generating highly recombined genes and evolved enzymes
-
Coco, W. M., Levinson, W. E., Crist, M. J., Hektor, H. J., Darzins, A., Pienkos, P. T., Squires, C. H., and Monticello, D. J. 2001. DNA shuffling method for generating highly recombined genes and evolved enzymes. Nat Biotechnol. 19(4): 354-359.
-
(2001)
Nat Biotechnol
, vol.19
, Issue.4
, pp. 354-359
-
-
Coco, W.M.1
Levinson, W.E.2
Crist, M.J.3
Hektor, H.J.4
Darzins, A.5
Pienkos, P.T.6
Squires, C.H.7
Monticello, D.J.8
-
26
-
-
0036714679
-
Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferase
-
Cohen, H. M., Tawfik, D. S. and Griffiths A. D. 2002. Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferase. Nucleic Acids Res. 30(17): 3880-3885.
-
(2002)
Nucleic Acids Res
, vol.30
, Issue.17
, pp. 3880-3885
-
-
Cohen, H.M.1
Tawfik, D.S.2
Griffiths, A.D.3
-
27
-
-
0842313326
-
Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization
-
Cohen, H. M., Tawfik, D. S., and Griffiths, A. D. 2004. Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization. Protein Eng Des Sel. 17(1): 3-11.
-
(2004)
Protein Eng des Sel
, vol.17
, Issue.1
, pp. 3-11
-
-
Cohen, H.M.1
Tawfik, D.S.2
Griffiths, A.D.3
-
28
-
-
0034950822
-
Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase
-
Delagrave, S., Murphy, D. J., Pruss, J. L., Maffia, A. M., Marrs, B. L., Bylina, E. J., Coleman, W. J., Grek, C. L., Dilworth, M. R., Yang, M. M., and Youvan, D. C. 2001. Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase. Protein Eng. 14(4): 261-267.
-
(2001)
Protein Eng
, vol.14
, Issue.4
, pp. 261-267
-
-
Delagrave, S.1
Murphy, D.J.2
Pruss, J.L.3
Maffia, A.M.4
Marrs, B.L.5
Bylina, E.J.6
Coleman, W.J.7
Grek, C.L.8
Dilworth, M.R.9
Yang, M.M.10
Youvan, D.C.11
-
29
-
-
0035313249
-
Enzymes from extremophiles
-
Demirjian, D. C., Moris-Varas, F., and Cassidy, C. S. 2001. Enzymes from extremophiles. Curr Opin Chem Biol. 5(2): 144-151.
-
(2001)
Curr Opin Chem Biol
, vol.5
, Issue.2
, pp. 144-151
-
-
Demirjian, D.C.1
Moris-Varas, F.2
Cassidy, C.S.3
-
30
-
-
0022912910
-
A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotides
-
Derbyshire, K. M., Salvo, J. J., and Grindley, N. D. 1986. A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotides. Gene. 46(2-3): 145-152.
-
(1986)
Gene
, vol.46
, Issue.2-3
, pp. 145-152
-
-
Derbyshire, K.M.1
Salvo, J.J.2
Grindley, N.D.3
-
31
-
-
3042855579
-
In vitro selection of restriction endonucleases by in vitro compartmentalization
-
Doi, N., Kumadaki, S., Oishi, Y., Matsumura, N., and Yanagawa, H. 2004. In vitro selection of restriction endonucleases by in vitro compartmentalization. Nucleic Acids Res. 32(12): e95-103.
-
(2004)
Nucleic Acids Res
, vol.32
, Issue.12
-
-
Doi, N.1
Kumadaki, S.2
Oishi, Y.3
Matsumura, N.4
Yanagawa, H.5
-
32
-
-
33644507991
-
Directed evolution and substrate specificity profile of homing endonuclease I-Scel
-
Doyon, J. B., Pattanayak, V., Meyer, C. B., and Liu, D. R. 2006. Directed evolution and substrate specificity profile of homing endonuclease I-Scel. J Am Chem Soc. 128(7): 2477-2484.
-
(2006)
J Am Chem Soc
, vol.128
, Issue.7
, pp. 2477-2484
-
-
Doyon, J.B.1
Pattanayak, V.2
Meyer, C.B.3
Liu, D.R.4
-
33
-
-
30444439246
-
Directed evolution of Bacillus subtilis lipase a by use of enantiomeric phosphonate inhibitors: Crystal structures and phage display selection
-
Droge, M. J., Boersma, Y. L., van Pouderoyen, G., Vrenken, T. E., Ruggeberg, C. J., Reetz, M. T., Dijkstra, B. W., and Quax, W. J. 2006. Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection. ChemBioChem. 7(1): 149-157.
-
(2006)
ChemBioChem
, vol.7
, Issue.1
, pp. 149-157
-
-
Droge, M.J.1
Boersma, Y.L.2
Van Pouderoyen, G.3
Vrenken, T.E.4
Ruggeberg, C.J.5
Reetz, M.T.6
Dijkstra, B.W.7
Quax, W.J.8
-
34
-
-
16244380464
-
New enzymes for biotransformations
-
Faber, K., and Kroutil, W. 2005. New enzymes for biotransformations. Curr Opin Chem Biol. 9(2): 181-187.
-
(2005)
Curr Opin Chem Biol
, vol.9
, Issue.2
, pp. 181-187
-
-
Faber, K.1
Kroutil, W.2
-
35
-
-
4644252576
-
Protein engineering of toluene 4-monooxygenase of Pseudomonas mendocina KR1 for synthesizing 4-nitrocatechol from nitrobenzene
-
Fishman, A., Tao, Y., Bentley, W. E., and Wood, T. K. 2004. Protein engineering of toluene 4-monooxygenase of Pseudomonas mendocina KR1 for synthesizing 4-nitrocatechol from nitrobenzene. Biotechnol Bioeng. 87(6): 779-790.
-
(2004)
Biotechnol Bioeng
, vol.87
, Issue.6
, pp. 779-790
-
-
Fishman, A.1
Tao, Y.2
Bentley, W.E.3
Wood, T.K.4
-
36
-
-
0033768504
-
Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars
-
Fong, S., Machajewski, T. D., Mak, C. C., and Wong, C. 2000. Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars. Chem Biol. 7(11): 873-883.
-
(2000)
Chem Biol
, vol.7
, Issue.11
, pp. 873-883
-
-
Fong, S.1
Machajewski, T.D.2
Mak, C.C.3
Wong, C.4
-
37
-
-
17644394187
-
Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity
-
Fujii, R., Nakagawa, Y., Hiratake, J., Sogabe, A., and Sakata, K. 2005. Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity. Protein Eng Des Sel. 18(2): 93-101.
-
(2005)
Protein Eng des Sel
, vol.18
, Issue.2
, pp. 93-101
-
-
Fujii, R.1
Nakagawa, Y.2
Hiratake, J.3
Sogabe, A.4
Sakata, K.5
-
38
-
-
3843075198
-
Biphenyl dioxygenases: Functional versatilities and directed evolution
-
Furukawa, K., Suenaga, H., and Goto, M. 2004. Biphenyl dioxygenases: functional versatilities and directed evolution. J Bacteriol. 186(16): 5189-96.
-
(2004)
J Bacteriol
, vol.186
, Issue.16
, pp. 5189-5196
-
-
Furukawa, K.1
Suenaga, H.2
Goto, M.3
-
39
-
-
0035260476
-
Orthogonal combinatorial mutagenesis: A codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols
-
Gaytan, P., Yanez, J., Sanchez, F., and Soberon, X. 2001. Orthogonal combinatorial mutagenesis: A codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols. Nucleic Acids Res. 29(3): e9-17.
-
(2001)
Nucleic Acids Res
, vol.29
, Issue.3
-
-
Gaytan, P.1
Yanez, J.2
Sanchez, F.3
Soberon, X.4
-
40
-
-
0035836707
-
Directed evolution of polymerase function by compartmentalized self-replication
-
Ghadessy, F. J., Ong, J. L., and Holliger, P. 2001. Directed evolution of polymerase function by compartmentalized self-replication. Proc Natl Acad Sci USA. 98: 4552-4557.
-
(2001)
Proc Natl Acad Sci USA
, vol.98
, pp. 4552-4557
-
-
Ghadessy, F.J.1
Ong, J.L.2
Holliger, P.3
-
41
-
-
2542525398
-
Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution
-
Ghadessy, F. J., Ramsay, N., Boudsocq, F., Loakes, D., Brown, A., Iwai, S., Vaisman, A., Woodgate, R., and Holliger, P. 2004. Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution. Nat Biotechnol. 22(6): 755-759.
-
(2004)
Nat Biotechnol
, vol.22
, Issue.6
, pp. 755-759
-
-
Ghadessy, F.J.1
Ramsay, N.2
Boudsocq, F.3
Loakes, D.4
Brown, A.5
Iwai, S.6
Vaisman, A.7
Woodgate, R.8
Holliger, P.9
-
42
-
-
0037413687
-
Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization
-
Griffiths, A. D., and Tawfik, D. S. 2003. Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization. EMBO J. 22(1): 24-35.
-
(2003)
EMBO J
, vol.22
, Issue.1
, pp. 24-35
-
-
Griffiths, A.D.1
Tawfik, D.S.2
-
43
-
-
0029159746
-
Forced evolution of glutathione S-transferase to create a more efficient drug detoxication enzyme
-
Gulick, A. M., and Fahl, W. E. 1995. Forced evolution of glutathione S-transferase to create a more efficient drug detoxication enzyme. Proc Natl Acad Sci USA 92(18): 8140-8144.
-
(1995)
Proc Natl Acad Sci USA
, vol.92
, Issue.18
, pp. 8140-8144
-
-
Gulick, A.M.1
Fahl, W.E.2
-
44
-
-
1642494902
-
Engineering p-hydroxyphenylpyruvate dioxygenase to a p-hydroxymandelate synthase and evidence for the proposed benzene oxide intermediate in homogentisate formation
-
Gunsior, M., Ravel, J., Challis, G. L., and Townsend, C. A. 2004. Engineering p-hydroxyphenylpyruvate dioxygenase to a p-hydroxymandelate synthase and evidence for the proposed benzene oxide intermediate in homogentisate formation. Biochemistry. 43(3): 663-674.
-
(2004)
Biochemistry
, vol.43
, Issue.3
, pp. 663-674
-
-
Gunsior, M.1
Ravel, J.2
Challis, G.L.3
Townsend, C.A.4
-
45
-
-
0031215080
-
Isolation of novel ribozymes that ligate AMP-activated RNA substrates
-
Hager, A. J., and Szostak, J. W. 1997. Isolation of novel ribozymes that ligate AMP-activated RNA substrates. Chem Biol. 4(8): 607-617.
-
(1997)
Chem Biol
, vol.4
, Issue.8
, pp. 607-617
-
-
Hager, A.J.1
Szostak, J.W.2
-
46
-
-
18044366809
-
A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents
-
Hao, J., and Berry, A. 2004. A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents. Protein Eng Des Sel. 17(9): 689-697.
-
(2004)
Protein Eng des Sel
, vol.17
, Issue.9
, pp. 689-697
-
-
Hao, J.1
Berry, A.2
-
47
-
-
0035852623
-
Metabolic engineering of bacteria for environmental applications: Construction of Pseudomonas strains for biodegradation of 2-chlorotoluene
-
Haro, M. A., and de Lorenzo, V. 2000. Metabolic engineering of bacteria for environmental applications: construction of Pseudomonas strains for biodegradation of 2-chlorotoluene. J Biotechnol. 85(2): 103-113.
-
(2000)
J Biotechnol
, vol.85
, Issue.2
, pp. 103-113
-
-
Haro, M.A.1
De Lorenzo, V.2
-
48
-
-
33646927894
-
Laboratory-evolved vanadium chloroperoxidase exhibits 100-fold higher halogenating activity at alkaline pH: Catalytic effects from first and second coordination sphere mutations
-
Hasan, Z., Renirie, R., Kerkman, R., Ruijssenaars, H. J., Hartog, A. F., and Wever, R. 2006. Laboratory-evolved vanadium chloroperoxidase exhibits 100-fold higher halogenating activity at alkaline pH: Catalytic effects from first and second coordination sphere mutations. J Biol Chem. 281(14): 9738-9744.
-
(2006)
J Biol Chem
, vol.281
, Issue.14
, pp. 9738-9744
-
-
Hasan, Z.1
Renirie, R.2
Kerkman, R.3
Ruijssenaars, H.J.4
Hartog, A.F.5
Wever, R.6
-
49
-
-
1842479434
-
Biocatalysis and biotransformation: Probing the potential usefulness and the mechanisms of action of some novel biocatalysts
-
Hauer, B., and Roberts, S. M. 2004. Biocatalysis and biotransformation: probing the potential usefulness and the mechanisms of action of some novel biocatalysts. Curr Opin Chem Biol. 8: 103-105.
-
(2004)
Curr Opin Chem Biol
, vol.8
, pp. 103-105
-
-
Hauer, B.1
Roberts, S.M.2
-
50
-
-
0032853779
-
Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay
-
Henke, E., and Bornscheuer, U. T. 1999. Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay. Biol Chem. 380(7-8): 1029-1033.
-
(1999)
Biol Chem
, vol.380
, Issue.7-8
, pp. 1029-1033
-
-
Henke, E.1
Bornscheuer, U.T.2
-
51
-
-
0038799745
-
General method for sequence-independent site-directed chimeragenesis
-
Hiraga, K., and Arnold, F. H. 2003. General method for sequence-independent site-directed chimeragenesis. J Mol Biol. 330(2): 287-296.
-
(2003)
J Mol Biol
, vol.330
, Issue.2
, pp. 287-296
-
-
Hiraga, K.1
Arnold, F.H.2
-
52
-
-
21544473154
-
Directed evolution of D-sialic acid aldolase to L-3-deoxy-manno-2- octulosonic acid (L-KDO) aldolase
-
Hsu, C. C., Hong, Z., Wada, M., Franke, D., and Wong, C. H. 2005. Directed evolution of D-sialic acid aldolase to L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase. Proc Natl Acad Sci USA. 102(26): 9122-9126.
-
(2005)
Proc Natl Acad Sci USA
, vol.102
, Issue.26
, pp. 9122-9126
-
-
Hsu, C.C.1
Hong, Z.2
Wada, M.3
Franke, D.4
Wong, C.H.5
-
53
-
-
0034805753
-
Changing the substrate specificity of cytochrome c peroxidase using directed evolution
-
Iffland, A., Gendreizig, S., Tafelmeyer, P., and Johnsson, K. 2001. Changing the substrate specificity of cytochrome c peroxidase using directed evolution. Biochem Biophys Res Commun. 286(1): 126-132.
-
(2001)
Biochem Biophys Res Commun
, vol.286
, Issue.1
, pp. 126-132
-
-
Iffland, A.1
Gendreizig, S.2
Tafelmeyer, P.3
Johnsson, K.4
-
54
-
-
26444478237
-
A novel method of screening thrombin-inhibiting DNA aptamers using an evolution-mimicking algorithm
-
Ikebukuro, K., Okumura, Y., Sumikura, K., and Karube, I. 2005. A novel method of screening thrombin-inhibiting DNA aptamers using an evolution-mimicking algorithm. Nucleic Acids Res. 33(12): e108-e115.
-
(2005)
Nucleic Acids Res
, vol.33
, Issue.12
-
-
Ikebukuro, K.1
Okumura, Y.2
Sumikura, K.3
Karube, I.4
-
55
-
-
16244376449
-
Whole organism biocatalysis
-
Ishige, T., Honda, K., and Shimizu, S. 2005. Whole organism biocatalysis. Curr Opin Chem Biol. 9(2): 174-180.
-
(2005)
Curr Opin Chem Biol
, vol.9
, Issue.2
, pp. 174-180
-
-
Ishige, T.1
Honda, K.2
Shimizu, S.3
-
56
-
-
33750002494
-
-
Jacobsen, E. N., Pfaltz, A., and Yamamoto, H. (Eds.). Springer, Berlin
-
Jacobsen, E. N., Pfaltz, A., and Yamamoto, H. (Eds.). 1999. Comprehensive Asymetric Catalysis I-III. Springer, Berlin.
-
(1999)
Comprehensive Asymetric Catalysis I-III
-
-
-
57
-
-
0033976143
-
Directed evolution of enantioselective enzymes for organic chemistry
-
Jaeger, K. E., and Reetz, M. T. 2000. Directed evolution of enantioselective enzymes for organic chemistry. Curr Opin Chem Biol. 4(1): 68-73.
-
(2000)
Curr Opin Chem Biol
, vol.4
, Issue.1
, pp. 68-73
-
-
Jaeger, K.E.1
Reetz, M.T.2
-
58
-
-
3543076929
-
Enantioselective biocatalysis optimized by directed evolution
-
Jaeger, K. E., and Eggert, T. 2004. Enantioselective biocatalysis optimized by directed evolution. Curr Opin Biotechnol. 15(4): 305-313.
-
(2004)
Curr Opin Biotechnol
, vol.15
, Issue.4
, pp. 305-313
-
-
Jaeger, K.E.1
Eggert, T.2
-
59
-
-
0035009353
-
Directed evolution and creation of enantioselective biocatalysts
-
Jaeger, K. E., Eggert, T., Eipper, A., and Reetz, M. T. 2001. Directed evolution and creation of enantioselective biocatalysts. Appl Microbiol Biotechnol. 55(5): 519-530.
-
(2001)
Appl Microbiol Biotechnol
, vol.55
, Issue.5
, pp. 519-530
-
-
Jaeger, K.E.1
Eggert, T.2
Eipper, A.3
Reetz, M.T.4
-
60
-
-
0036307433
-
Analysis of shuffled gene libraries
-
Joern, J. M., Meinhold, P., and Arnold, F. H. 2002. Analysis of shuffled gene libraries. J Mol Biol. 316(3): 643-656.
-
(2002)
J Mol Biol
, vol.316
, Issue.3
, pp. 643-656
-
-
Joern, J.M.1
Meinhold, P.2
Arnold, F.H.3
-
61
-
-
0037399074
-
6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo
-
6- alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo. Chem Biol. 10(4): 313-317.
-
(2003)
Chem Biol
, vol.10
, Issue.4
, pp. 313-317
-
-
Juillerat, A.1
Gronemeyer, T.2
Keppler, A.3
Gendreizig, S.4
Pick, H.5
Vogel, H.6
Johnsson, K.7
-
62
-
-
0034730195
-
Directed evolution of a (βα)8-barrel enzyme to catalyze related reactions in two different metabolic pathways
-
Jurgens, C., Strom, A., Wegener, D., Hettwer, S., Wilmanns, M., and Sterner, R. 2000. Directed evolution of a (βα)8-barrel enzyme to catalyze related reactions in two different metabolic pathways. Proc Natl Acad Sci USA 97(18): 9925-9930.
-
(2000)
Proc Natl Acad Sci USA
, vol.97
, Issue.18
, pp. 9925-9930
-
-
Jurgens, C.1
Strom, A.2
Wegener, D.3
Hettwer, S.4
Wilmanns, M.5
Sterner, R.6
-
63
-
-
0042530128
-
Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance
-
Kim, Y. W., Choi, J. H., Kim, J. W., Park, C., Kim, J. W., Cha, H., Lee, S. B., Oh, B. H., Moon, T. W., and Park, K. H. 2003. Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance. Appl Environ Microbiol. 69(8): 4866-4874.
-
(2003)
Appl Environ Microbiol
, vol.69
, Issue.8
, pp. 4866-4874
-
-
Kim, Y.W.1
Choi, J.H.2
Kim, J.W.3
Park, C.4
Kim, J.W.5
Cha, H.6
Lee, S.B.7
Oh, B.H.8
Moon, T.W.9
Park, K.H.10
-
64
-
-
0036669678
-
Industrial enzyme applications
-
Kirk, O., Borchert, T. V., and Fuglsang, C. C. 2002. Industrial enzyme applications. Curr Opin Biotechnol. 13(4): 345-351.
-
(2002)
Curr Opin Biotechnol
, vol.13
, Issue.4
, pp. 345-351
-
-
Kirk, O.1
Borchert, T.V.2
Fuglsang, C.C.3
-
65
-
-
0031874757
-
Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase
-
Kumamaru, T., Suenaga, H., Mitsuoka, M., Watanabe, T., and Furukawa, K. 1998. Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase. Nat Biotechnol. 16(7): 663-666.
-
(1998)
Nat Biotechnol
, vol.16
, Issue.7
, pp. 663-666
-
-
Kumamaru, T.1
Suenaga, H.2
Mitsuoka, M.3
Watanabe, T.4
Furukawa, K.5
-
66
-
-
21244500925
-
Directed evolution of mammalian cytochrome P450 2B1: Mutations outside of the active site enhance the metabolism of several substrates, including the anticancer prodrugs cyclophosphamide and ifosfamide
-
Kumar, S., Chen, C. S., Waxman, D. J., and Halpert, J. R. 2005. Directed evolution of mammalian cytochrome P450 2B1: mutations outside of the active site enhance the metabolism of several substrates, including the anticancer prodrugs cyclophosphamide and ifosfamide. J Biol Chem. 280(20): 19569-19575.
-
(2005)
J Biol Chem
, vol.280
, Issue.20
, pp. 19569-19575
-
-
Kumar, S.1
Chen, C.S.2
Waxman, D.J.3
Halpert, J.R.4
-
67
-
-
13844297445
-
Directed evolution of Escherichia coli farnesyl diphosphate synthase (IspA) reveals novel structural determinants of chain length specificity
-
Lee, P. C., Petri, R., Mijts, B. N., Watts, K. T., and Schmidt-Dannert, C. 2005. Directed evolution of Escherichia coli farnesyl diphosphate synthase (IspA) reveals novel structural determinants of chain length specificity. Metab Eng. 7(1): 18-26.
-
(2005)
Metab Eng
, vol.7
, Issue.1
, pp. 18-26
-
-
Lee, P.C.1
Petri, R.2
Mijts, B.N.3
Watts, K.T.4
Schmidt-Dannert, C.5
-
68
-
-
0037112614
-
Investigating the target recognition of DNA cytosine-5 methyltransferase Hhal by library selection using in vitro compartmentalisation (IVC)
-
Lee, Y. F., Tawfik, D. S., and Griffiths, A. D. 2002. Investigating the target recognition of DNA cytosine-5 methyltransferase Hhal by library selection using in vitro compartmentalisation (IVC). Nucleic Acids Res. 30(22): 4937-4944.
-
(2002)
Nucleic Acids Res
, vol.30
, Issue.22
, pp. 4937-4944
-
-
Lee, Y.F.1
Tawfik, D.S.2
Griffiths, A.D.3
-
69
-
-
0038070162
-
Conversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: The role of alanine 230 in acceptor subsite +1
-
Leemhuis, H., Rozeboom, H. J., Wilbrink, M., Euverink, G. J., Dijkstra, B. W., and Dijkhuizen, L. 2003. Conversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: The role of alanine 230 in acceptor subsite +1. Biochemistry. 42(24): 7518-7526.
-
(2003)
Biochemistry
, vol.42
, Issue.24
, pp. 7518-7526
-
-
Leemhuis, H.1
Rozeboom, H.J.2
Wilbrink, M.3
Euverink, G.J.4
Dijkstra, B.W.5
Dijkhuizen, L.6
-
70
-
-
0004110428
-
-
CBS Publishers, New Delhi
-
Lehninger, A. L., Nelson, D. L., and Cox, M. M. 1993. Principles of Biochemistry. 2nd edition, CBS Publishers, New Delhi.
-
(1993)
Principles of Biochemistry. 2nd Edition
-
-
Lehninger, A.L.1
Nelson, D.L.2
Cox, M.M.3
-
71
-
-
20444484058
-
Catalytic asymmetric vinylation and dienylation of ketones
-
Li, H., and Walsh, P. J. 2005. Catalytic asymmetric vinylation and dienylation of ketones. J Am Chem Soc. 127(23): 8355-8361.
-
(2005)
J Am Chem Soc
, vol.127
, Issue.23
, pp. 8355-8361
-
-
Li, H.1
Walsh, P.J.2
-
72
-
-
0033136115
-
Functional expression of horseradish peroxidase in E. coli by directed evolution
-
Lin, Z., Thorsen, T., and Arnold, F. H. 1999. Functional expression of horseradish peroxidase in E. coli by directed evolution. Biotechnol Prog. 15(3): 467-471.
-
(1999)
Biotechnol Prog
, vol.15
, Issue.3
, pp. 467-471
-
-
Lin, Z.1
Thorsen, T.2
Arnold, F.H.3
-
73
-
-
0036656222
-
Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis
-
Lingen, B., Grotzinger, J., Kolter, D., Kula, M. R., and Pohl, M. 2002. Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis. Protein Eng. 15(7): 585-93.
-
(2002)
Protein Eng
, vol.15
, Issue.7
, pp. 585-593
-
-
Lingen, B.1
Grotzinger, J.2
Kolter, D.3
Kula, M.R.4
Pohl, M.5
-
74
-
-
0041977179
-
Alteration of the substrate specificity of benzoylformate decarboxylase from Pseudomonas putida by directed evolution
-
Lingen, B., Kolter-Jung, D., Dunkelmann, P., Feldmann, R., Grotzinger, J., Pohl, M., and Muller, M. 2003. Alteration of the substrate specificity of benzoylformate decarboxylase from Pseudomonas putida by directed evolution. ChemBioChem. 4(8): 721-726.
-
(2003)
ChemBioChem
, vol.4
, Issue.8
, pp. 721-726
-
-
Lingen, B.1
Kolter-Jung, D.2
Dunkelmann, P.3
Feldmann, R.4
Grotzinger, J.5
Pohl, M.6
Muller, M.7
-
75
-
-
0035866186
-
Rapid generation of incremental truncation libraries for protein engineering using α-phosphothioate nucleotides
-
Lutz, S., Ostermeier, M., and Benkovic, S. J. 2001. Rapid generation of incremental truncation libraries for protein engineering using α-phosphothioate nucleotides. Nucleic Acids Res. 29(4): e16-22.
-
(2001)
Nucleic Acids Res
, vol.29
, Issue.4
-
-
Lutz, S.1
Ostermeier, M.2
Benkovic, S.J.3
-
76
-
-
0035949702
-
Creating multiple-crossover DNA libraries independent of sequence identity
-
Lutz, S., Ostermeier, M., Moore, G. L., Maranas, C. D., and Benkovic, S. J. 2001. Creating multiple-crossover DNA libraries independent of sequence identity. Proc Natl Acad Sci USA. 98(20): 11248-11253.
-
(2001)
Proc Natl Acad Sci USA
, vol.98
, Issue.20
, pp. 11248-11253
-
-
Lutz, S.1
Ostermeier, M.2
Moore, G.L.3
Maranas, C.D.4
Benkovic, S.J.5
-
77
-
-
0033054580
-
Directed evolution of the surface chemistry of the reporter enzyme β-glucuronidase
-
Matsumura, I., Wallingford, J. B., Surana, N. K., Vize, P. D., and Ellington, A. D. 1999. Directed evolution of the surface chemistry of the reporter enzyme β-glucuronidase. Nat Biotechnol. 17(7): 696-701.
-
(1999)
Nat Biotechnol
, vol.17
, Issue.7
, pp. 696-701
-
-
Matsumura, I.1
Wallingford, J.B.2
Surana, N.K.3
Vize, P.D.4
Ellington, A.D.5
-
78
-
-
0034059496
-
Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine
-
May, O., Nguyen, P. T., and Arnold, F. H. 2000. Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine. Nat Biotechnol. 18(3): 317-20.
-
(2000)
Nat Biotechnol
, vol.18
, Issue.3
, pp. 317-320
-
-
May, O.1
Nguyen, P.T.2
Arnold, F.H.3
-
79
-
-
0347130091
-
Growth of Escherichia coli coexpressing phosphotriesterase and glycerophosphodiester phosphodiesterase, using paraoxon as the sole phosphorus source
-
McLoughlin, S. Y., Jackson, C., Liu, J. W., and Ollis, D. L. 2004. Growth of Escherichia coli coexpressing phosphotriesterase and glycerophosphodiester phosphodiesterase, using paraoxon as the sole phosphorus source. Appl Environ Microbiol. 70(1): 404-412.
-
(2004)
Appl Environ Microbiol
, vol.70
, Issue.1
, pp. 404-412
-
-
McLoughlin, S.Y.1
Jackson, C.2
Liu, J.W.3
Ollis, D.L.4
-
80
-
-
0038731100
-
Library analysis of SCHEMA-guided protein recombination
-
Meyer, M. M., Silberg, J. J., Voigt, C. A., Endelman, J. B., Mayo, S. L., Wang, Z. G., and Arnold, F. H. 2003. Library analysis of SCHEMA-guided protein recombination. Protein Sci. 12(8): 1686-1693.
-
(2003)
Protein Sci
, vol.12
, Issue.8
, pp. 1686-1693
-
-
Meyer, M.M.1
Silberg, J.J.2
Voigt, C.A.3
Endelman, J.B.4
Mayo, S.L.5
Wang, Z.G.6
Arnold, F.H.7
-
81
-
-
17444391267
-
In vitro evolution and characterization of a ligase ribozyme adapted to acidic conditions: Effect of further rounds of evolution
-
Miyamoto, Y., Teramoto, N., Imanishi, Y., and Ito, Y. 2005. In vitro evolution and characterization of a ligase ribozyme adapted to acidic conditions: Effect of further rounds of evolution. Biotechnol Bioeng. 90(1): 36-45.
-
(2005)
Biotechnol Bioeng
, vol.90
, Issue.1
, pp. 36-45
-
-
Miyamoto, Y.1
Teramoto, N.2
Imanishi, Y.3
Ito, Y.4
-
82
-
-
33744515651
-
Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution
-
Miyazaki, K., Takenouchi, M., Kondo, H., Noro, N., Suzuki, M., and Tsuda, S. 2006. Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution. J Biol Chem. 281(15): 10236-10242.
-
(2006)
J Biol Chem
, vol.281
, Issue.15
, pp. 10236-10242
-
-
Miyazaki, K.1
Takenouchi, M.2
Kondo, H.3
Noro, N.4
Suzuki, M.5
Tsuda, S.6
-
83
-
-
0029670577
-
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents
-
Moore, J. C., and Arnold, F. H. 1996. Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents. Nat Biotechnol. 14(4): 458-467.
-
(1996)
Nat Biotechnol
, vol.14
, Issue.4
, pp. 458-467
-
-
Moore, J.C.1
Arnold, F.H.2
-
84
-
-
0034088553
-
Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris
-
Morawski, B., Lin, Z., Cirino, P., Joo, H., Bandara, G., and Arnold, F. H. 2000. Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. Protein Eng. 13(5): 377-384.
-
(2000)
Protein Eng
, vol.13
, Issue.5
, pp. 377-384
-
-
Morawski, B.1
Lin, Z.2
Cirino, P.3
Joo, H.4
Bandara, G.5
Arnold, F.H.6
-
85
-
-
0034842869
-
Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae
-
Morawski, B., Quan, S., and Arnold, F. H. 2001. Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae. Biotechnol Bioeng. 76(2): 99-107.
-
(2001)
Biotechnol Bioeng
, vol.76
, Issue.2
, pp. 99-107
-
-
Morawski, B.1
Quan, S.2
Arnold, F.H.3
-
86
-
-
0242286152
-
Mutations of penicillin acylase residue B71 extend substrate specificity by decreasing steric constraints for substrate binding
-
Morillas, M., McVey, C. E., Brannigan, J. A., Ladurner, A. G., Forney, L. J., and Virden, R. 2003. Mutations of penicillin acylase residue B71 extend substrate specificity by decreasing steric constraints for substrate binding. Biochem J. 371(Pt 1): 143-150.
-
(2003)
Biochem J
, vol.371
, Issue.1
, pp. 143-150
-
-
Morillas, M.1
McVey, C.E.2
Brannigan, J.A.3
Ladurner, A.G.4
Forney, L.J.5
Virden, R.6
-
87
-
-
0036901312
-
Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently
-
Ness, J. E., Kim, S., Gottman, A., Pak, R., Krebber, A., Borchert, T. V., Govindarajan, S., Mundorff, E. C., and Minshull, J. 2002. Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently. Nat Biotechnol. 20(12): 1251-1255.
-
(2002)
Nat Biotechnol
, vol.20
, Issue.12
, pp. 1251-1255
-
-
Ness, J.E.1
Kim, S.2
Gottman, A.3
Pak, R.4
Krebber, A.5
Borchert, T.V.6
Govindarajan, S.7
Mundorff, E.C.8
Minshull, J.9
-
88
-
-
16244421342
-
In vitro selection of functional nucleic acid sequences
-
Horton, R.M., and Tait, R.C., Eds. Horizon Scientific Press, UK
-
Nieuwlandt, D. 1998. In vitro selection of functional nucleic acid sequences. In: Genetic Engineering with PCR. pp. 117-132. Horton, R.M., and Tait, R.C., Eds. Horizon Scientific Press, UK.
-
(1998)
Genetic Engineering with PCR
, pp. 117-132
-
-
Nieuwlandt, D.1
-
89
-
-
0036960601
-
Structure-based combinatorial protein engineering (SCOPE)
-
O'Maille, P. E., Bakhtina, M., and Tsai, M. D. 2002. Structure-based combinatorial protein engineering (SCOPE). J Mol Biol. 321(4): 677-691.
-
(2002)
J Mol Biol
, vol.321
, Issue.4
, pp. 677-691
-
-
O'Maille, P.E.1
Bakhtina, M.2
Tsai, M.D.3
-
90
-
-
0036699655
-
Improvement of oxidative and thermostability of N-carbamyl-d-amino acid amidohydrolase by directed evolution
-
Oh, K. H., Nam, S. H., and Kim, H. S. 2002. Improvement of oxidative and thermostability of N-carbamyl-d-amino acid amidohydrolase by directed evolution. Protein Eng. 15(8): 689-695.
-
(2002)
Protein Eng
, vol.15
, Issue.8
, pp. 689-695
-
-
Oh, K.H.1
Nam, S.H.2
Kim, H.S.3
-
91
-
-
33644900247
-
Diversification and specialization of HIV protease function during in vitro evolution
-
O'Loughlin, T. L., Greene, D. N., and Matsumura, I. 2006. Diversification and specialization of HIV protease function during in vitro evolution. Mol Biol Evol. 23(4): 764-772.
-
(2006)
Mol Biol Evol
, vol.23
, Issue.4
, pp. 764-772
-
-
O'Loughlin, T.L.1
Greene, D.N.2
Matsumura, I.3
-
92
-
-
0035122917
-
Predicting the emergence of antibiotic resistance by directed evolution and structural analysis
-
Orencia, M. C., Yoon, J. S., Ness, J. E., Stemmer, W. P., and Stevens, R. C. 2001. Predicting the emergence of antibiotic resistance by directed evolution and structural analysis. Nat Struct Biol. 8(3): 238-242.
-
(2001)
Nat Struct Biol
, vol.8
, Issue.3
, pp. 238-242
-
-
Orencia, M.C.1
Yoon, J.S.2
Ness, J.E.3
Stemmer, W.P.4
Stevens, R.C.5
-
93
-
-
0037420903
-
Theoretical distribution of truncation lengths in incremental truncation libraries
-
Ostermeier, M. 2003. Theoretical distribution of truncation lengths in incremental truncation libraries. Biotechnol Bioeng. 82(5): 564-577.
-
(2003)
Biotechnol Bioeng
, vol.82
, Issue.5
, pp. 564-577
-
-
Ostermeier, M.1
-
94
-
-
0032850532
-
Incremental truncation as a strategy in the engineering of novel biocatalysts
-
Ostermeier, M., Nixon, A. E. and Benkovic, S. J. 1999. Incremental truncation as a strategy in the engineering of novel biocatalysts. Bioorg Med Chem. 7(10): 2139-2144.
-
(1999)
Bioorg Med Chem
, vol.7
, Issue.10
, pp. 2139-2144
-
-
Ostermeier, M.1
Nixon, A.E.2
Benkovic, S.J.3
-
95
-
-
17444363724
-
A combinatorial approach to hybrid enzymes independent of DNA homology
-
Ostermeier, M., Shim, J. H., and Benkovic, S. J. 1999. A combinatorial approach to hybrid enzymes independent of DNA homology. Nat Biotechnol. 17(12): 1205-1209.
-
(1999)
Nat Biotechnol
, vol.17
, Issue.12
, pp. 1205-1209
-
-
Ostermeier, M.1
Shim, J.H.2
Benkovic, S.J.3
-
96
-
-
32544436092
-
Preparation of human metabolites of propranolol using laboratory-evolved bacterial cytochromes P450
-
Otey, C. R., Bandara, G., Lalonde, J., Takahashi, K., and Arnold, F. H. 2006. Preparation of human metabolites of propranolol using laboratory-evolved bacterial cytochromes P450. Biotechnol Bioeng. 93(3): 494-499.
-
(2006)
Biotechnol Bioeng
, vol.93
, Issue.3
, pp. 494-499
-
-
Otey, C.R.1
Bandara, G.2
Lalonde, J.3
Takahashi, K.4
Arnold, F.H.5
-
97
-
-
0036829913
-
Altering the substrate specificity of cephalosporin acylase by directed evolution of the β-subunit
-
Otten, L. G., Sio, C. F., Vrielink, J., Cool, R. H., and Quax, W. J. 2002. Altering the substrate specificity of cephalosporin acylase by directed evolution of the β-subunit. J Biol Chem. 277(44): 42121-42127.
-
(2002)
J Biol Chem
, vol.277
, Issue.44
, pp. 42121-42127
-
-
Otten, L.G.1
Sio, C.F.2
Vrielink, J.3
Cool, R.H.4
Quax, W.J.5
-
98
-
-
33644980943
-
Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E. coli
-
Parikh, M. R., Greene, D. N., Woods, K. K., and Matsumura, I. 2006. Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E. coli. Protein Eng Des Sel. 19(3): 113-119.
-
(2006)
Protein Eng des Sel
, vol.19
, Issue.3
, pp. 113-119
-
-
Parikh, M.R.1
Greene, D.N.2
Woods, K.K.3
Matsumura, I.4
-
99
-
-
12344337569
-
Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations
-
Park, S., Morley, K. L., Horsman, G. P., Holmquist, M., Hult, K., and Kazlauskas, R. J. 2005. Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations. Chem Biol. 12(1): 45-54.
-
(2005)
Chem Biol
, vol.12
, Issue.1
, pp. 45-54
-
-
Park, S.1
Morley, K.L.2
Horsman, G.P.3
Holmquist, M.4
Hult, K.5
Kazlauskas, R.J.6
-
100
-
-
22544456237
-
In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase
-
Patrick, W. M., and Blackburn, J. M. 2005. In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase. FEBS J. 272(14): 3684-3697.
-
(2005)
FEBS J
, vol.272
, Issue.14
, pp. 3684-3697
-
-
Patrick, W.M.1
Blackburn, J.M.2
-
101
-
-
0242330792
-
Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3
-
Peters, M. W., Meinhold, P., Glieder, A., and Arnold, F. H. 2003. Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3. J AmChem Soc. 125(44): 13442-13450.
-
(2003)
J Am Chem Soc
, vol.125
, Issue.44
, pp. 13442-13450
-
-
Peters, M.W.1
Meinhold, P.2
Glieder, A.3
Arnold, F.H.4
-
102
-
-
0033908030
-
Designed evolution of enzymatic properties
-
Petrounia, I. P., and Arnold, F. H. 2000. Designed evolution of enzymatic properties. Curr Opin Biotechnol. 11(4): 325-330.
-
(2000)
Curr Opin Biotechnol
, vol.11
, Issue.4
, pp. 325-330
-
-
Petrounia, I.P.1
Arnold, F.H.2
-
103
-
-
16244419795
-
A general model of error-prone PCR
-
Pritchard, L., Corne, D., Kell, D., Rowland, J., and Winson, M. 2005. A general model of error-prone PCR. J Theor Biol. 234(4): 497-509.
-
(2005)
J Theor Biol
, vol.234
, Issue.4
, pp. 497-509
-
-
Pritchard, L.1
Corne, D.2
Kell, D.3
Rowland, J.4
Winson, M.5
-
104
-
-
21244447503
-
Application of the "codon-shuffling" method: Synthesis and selection of de novo proteins as antibacterials
-
Rao, A., Chopra, S., Ram, G., Gupta, A., and Ranganathan, A. 2005. Application of the "codon-shuffling" method: Synthesis and selection of de novo proteins as antibacterials. J Biol Chem. 280(25): 23605-23614.
-
(2005)
J Biol Chem
, vol.280
, Issue.25
, pp. 23605-23614
-
-
Rao, A.1
Chopra, S.2
Ram, G.3
Gupta, A.4
Ranganathan, A.5
-
105
-
-
0034583247
-
Application of directed evolution in the development of enantioselective enzymes
-
Reetz, M. T. 2000. Application of directed evolution in the development of enantioselective enzymes. Pure Appl Chem. 72(9): 1615-1622.
-
(2000)
Pure Appl Chem
, vol.72
, Issue.9
, pp. 1615-1622
-
-
Reetz, M.T.1
-
106
-
-
0141888961
-
An overview of high-throughput screening systems for enantioselective enzymatic transformations
-
Reetz, M. T. 2003. An overview of high-throughput screening systems for enantioselective enzymatic transformations. Methods Mol Biol. 230: 259-282.
-
(2003)
Methods Mol Biol
, vol.230
, pp. 259-282
-
-
Reetz, M.T.1
-
107
-
-
1942503297
-
Controlling the enantioselectivity of enzymes by directed evolution: Practical and theoretical ramifications
-
Reetz, M. T. 2004. Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications. Proc Natl Acad Sci USA. 101(16): 5716-5722.
-
(2004)
Proc Natl Acad Sci USA
, vol.101
, Issue.16
, pp. 5716-5722
-
-
Reetz, M.T.1
-
108
-
-
0037252787
-
REBASE: Restriction enzymes and methyltransferases
-
Roberts, R. J., Vincze, T., Posfai, J., and Macelis, D. 2003. REBASE: restriction enzymes and methyltransferases. Nucleic Acids Res. 31(1): 418-420.
-
(2003)
Nucleic Acids Res
, vol.31
, Issue.1
, pp. 418-420
-
-
Roberts, R.J.1
Vincze, T.2
Posfai, J.3
Macelis, D.4
-
109
-
-
1342310509
-
Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase
-
Rothman, S. C., Voorhies, M., and Kirsch, J. F. 2004. Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase. Protein Sci. 13(3): 763-772.
-
(2004)
Protein Sci
, vol.13
, Issue.3
, pp. 763-772
-
-
Rothman, S.C.1
Voorhies, M.2
Kirsch, J.F.3
-
110
-
-
85088715566
-
Chiral roundup
-
Rouhi, M. A. 2002. Chiral roundup. Chem Eng News. 80(23): 43-50.
-
(2002)
Chem Eng News
, vol.80
, Issue.23
, pp. 43-50
-
-
Rouhi, M.A.1
-
111
-
-
8744282801
-
Active site engineering of the epoxide hydrolase from Agrobacterium radiobacter AD1 to enhance aerobic mineralization of cis-1,2-dichloroethylene in cells expressing an evolved toluene orthomonooxygenase
-
Rui, L., Cao, L., Chen, W., Reardon, K. F., and Wood, T. K. 2004. Active site engineering of the epoxide hydrolase from Agrobacterium radiobacter AD1 to enhance aerobic mineralization of cis-1,2-dichloroethylene in cells expressing an evolved toluene orthomonooxygenase. J Biol Chem. 279(45): 46810-46817.
-
(2004)
J Biol Chem
, vol.279
, Issue.45
, pp. 46810-46817
-
-
Rui, L.1
Cao, L.2
Chen, W.3
Reardon, K.F.4
Wood, T.K.5
-
112
-
-
1942539970
-
Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione S-transferase, an evolved toluene o-monooxygenase, and γ-glutamylcysteine synthetase
-
Rui, L., Kwon, Y. M., Reardon, K. F., and Wood, T. K. 2004. Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione S-transferase, an evolved toluene o-monooxygenase, and γ-glutamylcysteine synthetase. Environ Microbiol. 6(5): 491-500.
-
(2004)
Environ Microbiol
, vol.6
, Issue.5
, pp. 491-500
-
-
Rui, L.1
Kwon, Y.M.2
Reardon, K.F.3
Wood, T.K.4
-
113
-
-
0342467880
-
Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution
-
Saab-Rincon, G., Juarez, V. R., Osuna, J., Sanchez, F., and Soberon, X. 2001. Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution. Protein Eng. 14(3): 149-155.
-
(2001)
Protein Eng
, vol.14
, Issue.3
, pp. 149-155
-
-
Saab-Rincon, G.1
Juarez, V.R.2
Osuna, J.3
Sanchez, F.4
Soberon, X.5
-
114
-
-
0035464767
-
Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate
-
Sakamoto, T., Joern, J. M., Arisawa, A., and Arnold, F. H. 2001. Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate. Appl Environ Microbiol. 67(9): 3882-3887.
-
(2001)
Appl Environ Microbiol
, vol.67
, Issue.9
, pp. 3882-3887
-
-
Sakamoto, T.1
Joern, J.M.2
Arisawa, A.3
Arnold, F.H.4
-
115
-
-
0141460411
-
Thermostabilization of a cytochrome p450 peroxygenase
-
Salazar, O., Cirino, P. C., and Arnold, F. H. 2003. Thermostabilization of a cytochrome p450 peroxygenase. ChemBioChem. 4(9): 891-893.
-
(2003)
ChemBioChem
, vol.4
, Issue.9
, pp. 891-893
-
-
Salazar, O.1
Cirino, P.C.2
Arnold, F.H.3
-
116
-
-
0037007010
-
Directed evolution of the site specificity of Cre recombinase
-
Santoro, S. W., and Schultz, P. G. 2002. Directed evolution of the site specificity of Cre recombinase. Proc Natl Acad Sci USA. 99(7): 4185-4190.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, Issue.7
, pp. 4185-4190
-
-
Santoro, S.W.1
Schultz, P.G.2
-
117
-
-
0034820028
-
Directed evolution to increase camptothecin sensitivity of human DNA topoisomerase I
-
Scaldaferro, S., Tinelli, S., Borgnetto, M. E., Azzini, A., and Capranico, G. 2001. Directed evolution to increase camptothecin sensitivity of human DNA topoisomerase I. Chem Biol. 8(9): 871-881.
-
(2001)
Chem Biol
, vol.8
, Issue.9
, pp. 871-881
-
-
Scaldaferro, S.1
Tinelli, S.2
Borgnetto, M.E.3
Azzini, A.4
Capranico, G.5
-
118
-
-
0035945238
-
Directed evolution of a recombinase for improved genomic integration at a native human sequence
-
Sclimenti, C. R., Thyagarajan, B., and Calos, M. P. 2001. Directed evolution of a recombinase for improved genomic integration at a native human sequence. Nucleic Acids Res. 29(24): 5044-5051.
-
(2001)
Nucleic Acids Res
, vol.29
, Issue.24
, pp. 5044-5051
-
-
Sclimenti, C.R.1
Thyagarajan, B.2
Calos, M.P.3
-
119
-
-
0842278671
-
Laboratory evolution of cytochrome p450 BM-3 monooxygenase for organic cosolvents
-
Seng Wong, T., Arnold, F. H., and Schwaneberg, U. 2004. Laboratory evolution of cytochrome p450 BM-3 monooxygenase for organic cosolvents. Biotechnol Bioeng. 85(3): 351-358.
-
(2004)
Biotechnol Bioeng
, vol.85
, Issue.3
, pp. 351-358
-
-
Seng Wong, T.1
Arnold, F.H.2
Schwaneberg, U.3
-
120
-
-
0035025314
-
Libraries of hybrid proteins from distantly related sequences
-
Sieber, V., Martinez, C. A., and Arnold, F. H. 2001. Libraries of hybrid proteins from distantly related sequences. Nat Biotechnol. 19(5): 456-460.
-
(2001)
Nat Biotechnol
, vol.19
, Issue.5
, pp. 456-460
-
-
Sieber, V.1
Martinez, C.A.2
Arnold, F.H.3
-
121
-
-
20144364220
-
Discovery of pectin-degrading enzymes and directed evolution of a novel pectate lyase for processing cotton fabric
-
Solbak, A. I., Richardson, T. H., McCann, R. T., Kline, K. A., Bartnek, F., Tomlinson, G., Tan, X., Parra-Gessert, L., Frey, G. J., Podar, M., Luginbuhl, P., Gray, K. A., Mathur, E. J., Robertson, D. E., Burk, M. J., Hazlewood, G. P., Short, J. M., and Kerovuo, J. 2005. Discovery of pectin-degrading enzymes and directed evolution of a novel pectate lyase for processing cotton fabric. J Biol Chem. 280(10): 9431-9438.
-
(2005)
J Biol Chem
, vol.280
, Issue.10
, pp. 9431-9438
-
-
Solbak, A.I.1
Richardson, T.H.2
McCann, R.T.3
Kline, K.A.4
Bartnek, F.5
Tomlinson, G.6
Tan, X.7
Parra-Gessert, L.8
Frey, G.J.9
Podar, M.10
Luginbuhl, P.11
Gray, K.A.12
Mathur, E.J.13
Robertson, D.E.14
Burk, M.J.15
Hazlewood, G.P.16
Short, J.M.17
Kerovuo, J.18
-
122
-
-
0034073503
-
Molecular determinants of xylose isomerase thermal stability and activity: Analysis of thermozymes by site-directed mutagenesis
-
Sriprapundh, D., Vieille, C., and Zeikus, J. G. 2000. Molecular determinants of xylose isomerase thermal stability and activity: analysis of thermozymes by site-directed mutagenesis. Protein Eng. 13(4): 259-265.
-
(2000)
Protein Eng
, vol.13
, Issue.4
, pp. 259-265
-
-
Sriprapundh, D.1
Vieille, C.2
Zeikus, J.G.3
-
123
-
-
0142215371
-
Directed evolution of Thermotoga neapolitana xylose isomerase: High activity on glucose at low temperature and low pH
-
Sriprapundh, D., Vieille, C., and Zeikus, J. G. 2003. Directed evolution of Thermotoga neapolitana xylose isomerase: High activity on glucose at low temperature and low pH. Protein Eng. 16(9): 683-690.
-
(2003)
Protein Eng
, vol.16
, Issue.9
, pp. 683-690
-
-
Sriprapundh, D.1
Vieille, C.2
Zeikus, J.G.3
-
124
-
-
0028110130
-
DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
-
Stemmer, W. P. C. 1994. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution. Proc Natl Acad Sci USA. 91(22): 10747-10751.
-
(1994)
Proc Natl Acad Sci USA
, vol.91
, Issue.22
, pp. 10747-10751
-
-
Stemmer, W.P.C.1
-
125
-
-
0028050350
-
Rapid evolution of a protein in vitro by DNA shuffling
-
Stemmer, W. P. C. 1994. Rapid evolution of a protein in vitro by DNA shuffling. Nature. 370(6488): 389-391.
-
(1994)
Nature
, vol.370
, Issue.6488
, pp. 389-391
-
-
Stemmer, W.P.C.1
-
126
-
-
0035183586
-
Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution
-
Sun, L., Petrounia, I. P., Yagasaki, M., Bandara, G., and Arnold, F. H. 2001. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng. 14(9): 699-704.
-
(2001)
Protein Eng
, vol.14
, Issue.9
, pp. 699-704
-
-
Sun, L.1
Petrounia, I.P.2
Yagasaki, M.3
Bandara, G.4
Arnold, F.H.5
-
127
-
-
2642565358
-
8-barrel enzyme into a split-protein sensor through directed evolution
-
8-barrel enzyme into a split-protein sensor through directed evolution. Chem Biol. 11(5): 681-689.
-
(2004)
Chem Biol
, vol.11
, Issue.5
, pp. 681-689
-
-
Tafelmeyer, P.1
Johnsson, N.2
Johnsson, K.3
-
128
-
-
0031854986
-
Man-made cell-like compartments for molecular evolution
-
Tawfik, D. S., and Griffiths, A. D. 1998. Man-made cell-like compartments for molecular evolution. Nat Biotechnol. 16(7): 652-656.
-
(1998)
Nat Biotechnol
, vol.16
, Issue.7
, pp. 652-656
-
-
Tawfik, D.S.1
Griffiths, A.D.2
-
129
-
-
24944477838
-
Designing catalysts for clean technology, green chemistry, and sustainable development
-
Thomas, J. M., and Raja, R. Designing catalysts for clean technology, green chemistry, and sustainable development. Annual Review of Materials Research 2005. 35: 315-350.
-
(2005)
Annual Review of Materials Research
, vol.35
, pp. 315-350
-
-
Thomas, J.M.1
Raja, R.2
-
130
-
-
0033809270
-
Directed evolution to improve the thermostability of prolyl endopeptidase
-
Uchiyama, H., Inaoka, T., Ohkuma-Soyejima, T., Togame, H., Shibanaka, Y., Yoshimoto, T., and Kokubo, T. 2000. Directed evolution to improve the thermostability of prolyl endopeptidase. J Biochem. 128(3): 441-447.
-
(2000)
J Biochem
, vol.128
, Issue.3
, pp. 441-447
-
-
Uchiyama, H.1
Inaoka, T.2
Ohkuma-Soyejima, T.3
Togame, H.4
Shibanaka, Y.5
Yoshimoto, T.6
Kokubo, T.7
-
131
-
-
4444306430
-
Directed evolution of enzymes for product chemistry
-
Valetti, F., and Gilardi, G. 2004. Directed evolution of enzymes for product chemistry. Nat Prod Rep. 21: 490-511.
-
(2004)
Nat Prod Rep
, vol.21
, pp. 490-511
-
-
Valetti, F.1
Gilardi, G.2
-
132
-
-
23644450225
-
Simultaneous optimization of enzyme activity and quaternary structure by directed evolution
-
Vamvaca, K., Butz, M., Walter, K. U., Taylor, S. V., and Hilvert, D. 2005. Simultaneous optimization of enzyme activity and quaternary structure by directed evolution. Protein Sci. 14(8): 2103-2114.
-
(2005)
Protein Sci
, vol.14
, Issue.8
, pp. 2103-2114
-
-
Vamvaca, K.1
Butz, M.2
Walter, K.U.3
Taylor, S.V.4
Hilvert, D.5
-
133
-
-
33644948874
-
Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution
-
van der Veen, B. A., Skov, L. K., Potocki-Veronese, G., Gajhede, M., Monsan, P., and Remaud-Simeon, M. 2006. Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution. FEBS J. 273(4): 673-681.
-
(2006)
FEBS J
, vol.273
, Issue.4
, pp. 673-681
-
-
Van Der Veen, B.A.1
Skov, L.K.2
Potocki-Veronese, G.3
Gajhede, M.4
Monsan, P.5
Remaud-Simeon, M.6
-
134
-
-
0034675342
-
Enhancement of the activity of I-aspartase from Escherichia coli W by directed evolution
-
Wang, L. J., Kong, X. D., Zhang, H. Y., Wang, X. P., and Zhang, J. 2000. Enhancement of the activity of I-aspartase from Escherichia coli W by directed evolution. Biochem Biophys Res Commun. 276(1): 346-349.
-
(2000)
Biochem Biophys Res Commun
, vol.276
, Issue.1
, pp. 346-349
-
-
Wang, L.J.1
Kong, X.D.2
Zhang, H.Y.3
Wang, X.P.4
Zhang, J.5
-
135
-
-
1842665130
-
Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution
-
Wilkinson, D., Akumanyi, N., Hurtado-Guerrero, R., Dawkes, H., Knowles, P. F., Phillips, S. E., and McPherson, M. J. 2004. Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution. Protein Eng Des Sel. 17(2): 141-148.
-
(2004)
Protein Eng des Sel
, vol.17
, Issue.2
, pp. 141-148
-
-
Wilkinson, D.1
Akumanyi, N.2
Hurtado-Guerrero, R.3
Dawkes, H.4
Knowles, P.F.5
Phillips, S.E.6
McPherson, M.J.7
-
136
-
-
0037453002
-
Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution
-
Williams, G. J., Domann, S., Nelson, A., and Berry, A. 2003. Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution. Proc Natl Acad Sci USA. 100(6): 3143-3148.
-
(2003)
Proc Natl Acad Sci USA
, vol.100
, Issue.6
, pp. 3143-3148
-
-
Williams, G.J.1
Domann, S.2
Nelson, A.3
Berry, A.4
-
137
-
-
23944490138
-
Enantioselective organocatalytic intramolecular Diels-Alder reactions: The asymmetric synthesis of solanapyrone D
-
Wilson, R. M., Jen, W. S., and Macmillan, D. W. 2005. Enantioselective organocatalytic intramolecular Diels-Alder reactions: The asymmetric synthesis of solanapyrone D. J Am Chem Soc. 127(33): 11616-11617.
-
(2005)
J Am Chem Soc
, vol.127
, Issue.33
, pp. 11616-11617
-
-
Wilson, R.M.1
Jen, W.S.2
Macmillan, D.W.3
-
138
-
-
0034644667
-
Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution
-
Wintrode, P. L., Miyazaki, K., and Arnold, F. H. 2000. Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution. J Biol Chem. 275(41): 31635-31640.
-
(2000)
J Biol Chem
, vol.275
, Issue.41
, pp. 31635-31640
-
-
Wintrode, P.L.1
Miyazaki, K.2
Arnold, F.H.3
-
139
-
-
2342535792
-
Sequence saturation mutagenesis (SeSaM): A novel method for directed evolution
-
Wong, T. S., Tee, K. L., Hauer, B., and Schwaneberg, U. 2004. Sequence saturation mutagenesis (SeSaM): A novel method for directed evolution. Nucleic Acids Research. 32(3): e26-34.
-
(2004)
Nucleic Acids Research
, vol.32
, Issue.3
-
-
Wong, T.S.1
Tee, K.L.2
Hauer, B.3
Schwaneberg, U.4
-
140
-
-
0035148643
-
Directed evolution of a new catalytic site in 2-keto-3-deoxy-6- phosphogluconate aldolase from Escherichia coli
-
Wymer, N., Buchanan, L. V., Henderson, D., Mehta, N., Botting, C. H., Pocivavsek, L., Fierke, C. A., Toone, E. J., and Naismith, J. H. 2001. Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. Structure (Camb). 9(1): 1-9.
-
(2001)
Structure (Camb)
, vol.9
, Issue.1
, pp. 1-9
-
-
Wymer, N.1
Buchanan, L.V.2
Henderson, D.3
Mehta, N.4
Botting, C.H.5
Pocivavsek, L.6
Fierke, C.A.7
Toone, E.J.8
Naismith, J.H.9
-
141
-
-
0037076311
-
Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase
-
Xia, G., Chen, L., Sera, T., Fa, M., Schultz, P. G., and Romesberg, F. E. 2002. Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase. Proc Natl Acad Sci USA. 99(10): 6597-6602.
-
(2002)
Proc Natl Acad Sci USA
, vol.99
, Issue.10
, pp. 6597-6602
-
-
Xia, G.1
Chen, L.2
Sera, T.3
Fa, M.4
Schultz, P.G.5
Romesberg, F.E.6
-
142
-
-
33344465082
-
Directed evolution of extradiol dioxygenase by a novel in vivo DNA shuffling
-
Xu, S., Ju, J., Misono, H., and Ohnishi, K. 2006. Directed evolution of extradiol dioxygenase by a novel in vivo DNA shuffling. Gene. 1(368): 126-137.
-
(2006)
Gene
, vol.1
, Issue.368
, pp. 126-137
-
-
Xu, S.1
Ju, J.2
Misono, H.3
Ohnishi, K.4
-
143
-
-
0042433116
-
New understandings of thermostable and peizostable enzymes
-
Yano, J. K., and Poulos, T. L. 2003. New understandings of thermostable and peizostable enzymes. Curr Opin Biotechnol. 14(4): 360-365.
-
(2003)
Curr Opin Biotechnol
, vol.14
, Issue.4
, pp. 360-365
-
-
Yano, J.K.1
Poulos, T.L.2
-
144
-
-
0032510667
-
Directed evolution of an aspartate aminotransferase with new substrate specificities
-
Yano, T., Oue, S., and Kagamiyama, H. 1998. Directed evolution of an aspartate aminotransferase with new substrate specificities. Proc Natl Acad Sci USA. 95(10): 5511-5515.
-
(1998)
Proc Natl Acad Sci USA
, vol.95
, Issue.10
, pp. 5511-5515
-
-
Yano, T.1
Oue, S.2
Kagamiyama, H.3
-
145
-
-
0029969577
-
Directed evolution of subtilisin e in Bacillus subtilis to enhance total activity in aqueous dimethylformamide
-
You, L., and Arnold, F. H. 1996. Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide. Protein Eng. 9(1): 77-83.
-
(1996)
Protein Eng
, vol.9
, Issue.1
, pp. 77-83
-
-
You, L.1
Arnold, F.H.2
-
146
-
-
0029869449
-
An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues
-
Zaccolo, M., Williams, D. M., Brown, D. M., and Gherardi, E. 1996. An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues. J Mol Biol. 255(4): 589-603.
-
(1996)
J Mol Biol
, vol.255
, Issue.4
, pp. 589-603
-
-
Zaccolo, M.1
Williams, D.M.2
Brown, D.M.3
Gherardi, E.4
-
147
-
-
0035313673
-
Industrial biocatalysis
-
Zaks, A. 2001. Industrial biocatalysis. Curr Opin Chem Biol. 5(2): 130-136.
-
(2001)
Curr Opin Chem Biol
, vol.5
, Issue.2
, pp. 130-136
-
-
Zaks, A.1
-
148
-
-
0037415021
-
Assembly of designed oligonucleotides as an efficient method for gene recombination: A new tool in directed evolution
-
Zha, D., Eipper, A., and Reetz, M. T. 2003. Assembly of designed oligonucleotides as an efficient method for gene recombination: A new tool in directed evolution. Chem Bio Chem. 4(1): 34-39.
-
(2003)
Chem Bio Chem
, vol.4
, Issue.1
, pp. 34-39
-
-
Zha, D.1
Eipper, A.2
Reetz, M.T.3
-
149
-
-
0141817121
-
Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution
-
Zhang, N., Suen, W. C., Windsor, W., Xiao, L., Madison, V., and Zaks, A. 2003. Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution. Protein Eng. 16(8): 599-605.
-
(2003)
Protein Eng
, vol.16
, Issue.8
, pp. 599-605
-
-
Zhang, N.1
Suen, W.C.2
Windsor, W.3
Xiao, L.4
Madison, V.5
Zaks, A.6
-
150
-
-
0031909113
-
Molecular evolution by staggered extension process (StEP) in vitro recombination
-
Zhao, H. M., Giver, L., Shao, Z. X., Affholter, J. A., and Arnold, F. H. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat Biotechnol. 16(3): 258-261.
-
Nat Biotechnol
, vol.16
, Issue.3
, pp. 258-261
-
-
Zhao, H.M.1
Giver, L.2
Shao, Z.X.3
Affholter, J.A.4
Arnold, F.H.5
-
151
-
-
33645823702
-
Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy
-
Zhou, M., Xu, H., Wei, X., Ye, Z., Wei, L., Gong, W., Wang, Y., and Zhu, Z. 2006. Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy. Plant Physiol. 40(1): 184-195.
-
(2006)
Plant Physiol
, vol.40
, Issue.1
, pp. 184-195
-
-
Zhou, M.1
Xu, H.2
Wei, X.3
Ye, Z.4
Wei, L.5
Gong, W.6
Wang, Y.7
Zhu, Z.8
-
152
-
-
25844504983
-
Critical review of development, validation, and transfer for high throughput bioanalytical LC-MS/MS Methods
-
Zhou, S., Song, Q., Tang, Y., and Naidong, W. 2005. Critical review of development, validation, and transfer for high throughput bioanalytical LC-MS/MS Methods. Current Pharmaceutical Analysis. 1(1): 3-14.
-
(2005)
Current Pharmaceutical Analysis
, vol.1
, Issue.1
, pp. 3-14
-
-
Zhou, S.1
Song, Q.2
Tang, Y.3
Naidong, W.4
-
153
-
-
0025988826
-
Random mutagenesis of gene-sized DNA molecule by use of PCR with Taq DNA polymerase
-
Zhou, Y. H., Zhang, X. P., and Ebright, R. H. 1991. Random mutagenesis of gene-sized DNA molecule by use of PCR with Taq DNA polymerase. Nucleic Acids Res. 19(21): 6052.
-
(1991)
Nucleic Acids Res
, vol.19
, Issue.21
, pp. 6052
-
-
Zhou, Y.H.1
Zhang, X.P.2
Ebright, R.H.3
-
154
-
-
33644941536
-
Generation of novel-substrate-accepting biphenyl dioxygenases through segmental random mutagenesis and identification of residues involved in enzyme specificity
-
Zielinski, M., Kahl, S., Standfuss-Gabisch, C., Camara, B., Seeger, M., and Hofer, B. 2006. Generation of novel-substrate-accepting biphenyl dioxygenases through segmental random mutagenesis and identification of residues involved in enzyme specificity. Appl Environ Microbiol. 72(3): 2191-2199.
-
(2006)
Appl Environ Microbiol
, vol.72
, Issue.3
, pp. 2191-2199
-
-
Zielinski, M.1
Kahl, S.2
Standfuss-Gabisch, C.3
Camara, B.4
Seeger, M.5
Hofer, B.6
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