Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface to Increase Multipoint Covalent Attachment

Applied and Environmental Microbiology

Volumn 70, Issue 2, 2004, Pages 1249-1251

  Abian, Olga ^a   Grazú, Valeria ^a   Hermoso, Juan ^b   González, Ramón ^c   García, José Luis ^d   Fernández Lafuente, Roberto ^a   Guisán, José Manuel ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

^c INSTITUTO DE FERMENTACIONES INDUSTRIALES CSIC   (Spain)

^d CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME IMMOBILIZATION; ENZYMES; ESCHERICHIA COLI; MUTAGENESIS;

ENZYME'S STABILITY; KINETIC PROPERTIES; MUTANTS;

MICROBIOLOGY;

AGAROSE; BACTERIAL ENZYME; LYSINE; MUTANT PROTEIN; N,N DIMETHYLFORMAMIDE; PENICILLIN G ACYLASE; UNCLASSIFIED DRUG;

MICROBIOLOGY;

ARTICLE; COVALENT BOND; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME STABILITY; ESCHERICHIA COLI; MUTATION; NONHUMAN; PH; SITE DIRECTED MUTAGENESIS; SURFACE PROPERTY; TEMPERATURE;

ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MUTAGENESIS, SITE-DIRECTED; MUTATION; PENICILLIN AMIDASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 2342591293     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.2.1249-1251.2004     Document Type: Article

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