Evidence for 'lock and key' character in an anti-phosphonate hydrolytic antibody catalytic site augmented by non-reaction centre recognition: Variation in substrate selectivity between an anti-phosphonate antibody, an anti-phosphate antibody and two hydrolytic enzymes

Biochemical Journal

Volumn 381, Issue 1, 2004, Pages 125-130

  Sonkaria, Sanjiv ^a,d   Boucher, Guillaume ^b,e   Flórez Álvarez, José ^a   Said, Bilal ^b,f   Hussain, Syeed ^a,g   Ostler, Elizabeth L ^b   Gul, Sheraz ^a,h   Thomas, Emrys W ^c   Resmini, Marina ^a   Gallacher, Gerard ^b   Brocklehurst, Keith ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF BRIGHTON   (United Kingdom)

^c UNIVERSITY OF SALFORD   (United Kingdom)

^d THROMBOSIS RESEARCH INSTITUTE   (United Kingdom)

^e UNIVERSITÉ DE NANTES   (France)

^f UNIVERSITY OF SHEFFIELD   (United Kingdom)

^g INSTITUTE OF CANCER RESEARCH   (United Kingdom)

^h GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Catalytic site rigidity; Hydrolytic catalytic antibody; Kinetic characterization; Lock and key; Substrate selectivity; Truncated carbonate and carboxylic ester substrate synthesis

Indexed keywords

BIOCHEMISTRY; CATALYSIS; ESTERS; HYDROLYSIS; PHOSPHATES;

DIFFERENTIAL KINETIC SELECTIVITIES; TRUNCATED SUBSTRATES;

ANTIBODIES;

4 NITROPHENOLATE; ANTIBODY; CARBONIC ACID; ESTER; HAPTEN; HYDROLASE; IMMUNOGLOBULIN G; PHENOL; PHOSPHATE ANTIBODY; PHOSPHONATE ANTIBODY; POLYCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; COMPARATIVE STUDY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ENZYME SYNTHESIS; HYDROLYSIS; KINETICS; MOLECULAR RECOGNITION; PRIORITY JOURNAL; REACTION ANALYSIS;

ANIMALS; ANTIBODIES, CATALYTIC; ANTIBODY AFFINITY; ANTIGEN-ANTIBODY REACTIONS; BINDING SITES, ANTIBODY; CATALYTIC DOMAIN; CATTLE; CHYMOTRYPSIN; ESTERASES; HYDROLASES; HYDROLYSIS; LIVER; MODELS, MOLECULAR; PANCREAS; PHOSPHATES; PHOSPHONIC ACIDS; SUBSTRATE SPECIFICITY; SWINE; VACCINES, SYNTHETIC;

EID: 3142729079     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20031966     Document Type: Article

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