Sol-gel immobilization of serine proteases for application in organic solvents

Biotechnology and Bioengineering

Volumn 75, Issue 2, 2001, Pages 154-158

  Van Unen, Dirk Jan ^a   Engbersen, Johan F J ^a   Reinhoudt, David N ^a  

^a UNIVERSITY OF TWENTE   (Netherlands)

Author keywords

Immobilization; Organic solvents; Serine proteases; Sol gel; Transesterification

Indexed keywords

ENZYMES; HYDROPHOBICITY; ORGANIC SOLVENTS; SOL-GELS;

ENZYME ACTIVITY;

CELL IMMOBILIZATION;

CHYMOTRYPSIN A; CYCLOHEXANE; ORGANIC SOLVENT; PHENYLALANINE DERIVATIVE; PROPANOL; SERINE PROTEINASE; SUBTILISIN; TRYPSIN;

ARTICLE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ESTERIFICATION; FREEZE DRYING; GEL; HYDROPHOBICITY;

CHROMATOGRAPHY, GAS; CHYMOTRYPSIN; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESTERIFICATION; KINETICS; PROTEIN CONFORMATION; SERINE ENDOPEPTIDASES; SILANES; SOLVENTS; SONICATION; SUBTILISIN; TRYPSIN;

EID: 0035922880     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1173     Document Type: Article

References (24)

1. Enzymes and other proteins entrapped in sol-gel materials
2. Effect of immiscible organic solvents on activity/stability of native chymotrypsin and immobilized-stabilized derivatives
3. Blueprint for a lipase support: Use of hydrophobic controlled-pore glasses as model systems
4. Large activation of serine proteases by pretreatment with crown ethers
5. Preservation of the native structure in myoglobin at low pH by sol-gel encapsulation
6. Encapsulation of proteins in transparant porous silicate glasses prepared by the sol-gel method
7. Encapsulation of biologicals within silicate, siloxane, and hybrid sol-gel polymers: An efficient and generic approach
8. On enzymatic activity in organic solvents as a function of enzyme history
9. A novel protocol to entrap active urease in a tetraethoxysilane-derived sol-gel thin-film architecture
10. Preparation of active and stable biocatalytic hydrogels for use in selective transformations
11. Practical route to high activity enzyme preparations for synthesis in organic media
12. Efficient heterogeneous biocatalysts for entrapment of lipases in hydrophobic sol-gel materials
13. Efficient immobilization of lipases by entrapment in hydrophobic sol-gel materials
14. Entrapment of biocatalysts in hydrophobic sol-gel materials for use in organic chemistry
15. Solvation of CBZ-amino acid nitrophenyl esters in organic media and the kinetics of their transesterification by subtilisin
16. Large acceleration of α-chymotrypsin-catalyzed dipeptide formation by 18-crown-6 in organic solvents
17. Crown ether activation of cross-linked subtilisin Carlsberg crystals in organic solvents
18. Improved activity retention of enzymes deposited on solid supports