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Enzyme Catalysis In Organic Synthesis
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Intermolecular cross-linking of a protein in the crystalline state: Carboxypeptidase-A
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Kinetic properties of carboxypeptidase B in solutions and crystals
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Alter GM, Leussing DL, Neurath H, Vallee BL Kinetic properties of carboxypeptidase B in solutions and crystals. Biochemistry. 16:1977;3663-3668.
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0000032049
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Cross-linked crystalline horse liver alcohol dehydrogenase as a redox catalyst: Activity and stability towards organic solvent
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Cross-linked enzyme crystals as robust biocatalysts
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St Clair NL, Navia MA Cross-linked enzyme crystals as robust biocatalysts. J Am Chem Soc. 114:1992;7314-7316.
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Cross-linked enzyme crystals (CLECs): Efficient and stable biocatalysts for preparative organic chemistry
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Zelinski T, Waldmann H Cross-linked enzyme crystals (CLECs): efficient and stable biocatalysts for preparative organic chemistry. Angew Chem Int Ed Engl. 36:1997;722-724.
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Zelinski T, Waldmann H Cross-linked enzyme crystals (CLECs). Powerful biocatalysts for synthetic chemistry. J Prakt Chem. 339:1997;394-396.
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Practical catalysis with enzyme crystals
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Lalonde J Practical catalysis with enzyme crystals. Chemtech. 27:1997;38-45.
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Cross-linked enzyme crystals of lipases as catalysts for kinetic resolution of acids and alcohols
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Lalonde JJ, Navia MA, Margolin AL Cross-linked enzyme crystals of lipases as catalysts for kinetic resolution of acids and alcohols. Methods Enzymol. 286:1997;443-464.
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Why are enzymes less active in organic solvents than in water?
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X-ray crystal structure of cross-linked subtilisin carlsberg in water vs. acetonitrile
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X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture
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This paper describes one of the few X-ray studies of an enzyme in an organic medium. The influence of water content on the protein conformation and flexibility is described. The results help us to understand the interaction of an organic solvent with the enzyme at the molecular level.
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Wang Z, Zhu G, Huang Q, Qian M, Shao M, Jia Y, Tang Y X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture. Biochim Biophys Acta. 1384:1998;335-344. This paper describes one of the few X-ray studies of an enzyme in an organic medium. The influence of water content on the protein conformation and flexibility is described. The results help us to understand the interaction of an organic solvent with the enzyme at the molecular level.
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Biochim Biophys Acta
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Wang, Z.1
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Enzymatic synthesis of nonracemic inherently chiral calix[4]arenes by lipase-catalysed transesterification
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Browne JK, McKervey MA, Pitarch M, Russell JA, Millership JS Enzymatic synthesis of nonracemic inherently chiral calix[4]arenes by lipase-catalysed transesterification. Tetrahedron Lett. 39:1998;1787-1790.
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19
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0028958932
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Cross-linked enzyme crystals (CLECs) of thermolysin in the synthesis of peptides
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Persichetti RA, St. Clair NL, Griffith JP, Navia MA, Margolin AL Cross-linked enzyme crystals (CLECs) of thermolysin in the synthesis of peptides. J Am Chem Soc. 117:1995;2732-2737.
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Persichetti, R.A.1
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20
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Application of structure-based thermodynamic calculations to the rationalization of the enantioselectivity of subtilisin in organic solvents
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Colombo G, Ottolina G, Carrea G, Bernardi A, Scolastico C Application of structure-based thermodynamic calculations to the rationalization of the enantioselectivity of subtilisin in organic solvents. Tetrahedron - Asymmetry. 9:1998;1205-1214.
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21
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0030914481
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Cross-linked crystals of subtilisin: A versatile catalyst for organic synthesis
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The application of cross-linkedcrystals of subtilisin Carlsberg in the synthesis of peptides or peptidomimetics, the hydrolysis of amino acid and peptide amides and the enantioselective hydrolysis of esters is described comprehensively. Because of the detailed experimental section, a good insight into the handling of cross-linked enzyme crystals is provided.
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Wang YF, Yakovlevsky K, Zhang B, Margolin AL Cross-linked crystals of subtilisin: a versatile catalyst for organic synthesis. J Org Chem. 62:1997;3488-3495. The application of cross-linkedcrystals of subtilisin Carlsberg in the synthesis of peptides or peptidomimetics, the hydrolysis of amino acid and peptide amides and the enantioselective hydrolysis of esters is described comprehensively. Because of the detailed experimental section, a good insight into the handling of cross-linked enzyme crystals is provided.
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J Org Chem
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Wang, Y.F.1
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Cross-linked enzyme crystals as highly active catalysts in organic solvents
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Khalaf N, Govardhan CP, Lalonde JJ, Persichetti RA, Wang YF, Margolin AL Cross-linked enzyme crystals as highly active catalysts in organic solvents. J Am Chem Soc. 118:1996;5494-5495.
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Khalaf, N.1
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Lalonde, J.J.3
Persichetti, R.A.4
Wang, Y.F.5
Margolin, A.L.6
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23
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0029883864
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The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents
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Schmitke JL, Wescott CR, Klibanov AM The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents. J Am Chem Soc. 118:1996;3360-3365.
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Schmitke, J.L.1
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24
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0000741925
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Cross-linked LDH crystals for lactate synthesis coupled to electroenzymatic regeneration of NADH
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Sobolov SB, Leonida MD, Bartiszko-Malik A, Voivodov KI, McKinney F, Kim J, Fry AJ Cross-linked LDH crystals for lactate synthesis coupled to electroenzymatic regeneration of NADH. J Org Chem. 64:1996;2125-2128.
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Sobolov, S.B.1
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25
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0344621439
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New biopolymers for electroenzymatic synthesis of alpha-hydroxy acids
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Fry AJ, Sobolov SB, Leonida MD, Voivodov KI, Fenton J New biopolymers for electroenzymatic synthesis of alpha-hydroxy acids. Electrochem Soc Proc. 97:1997;115-122.
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Electrochem Soc Proc
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Fry, A.J.1
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Voivodov, K.I.4
Fenton, J.5
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26
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0023732620
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Kaiser ET Catalytic activity of enzymes altered at their active sites. Angew Chem Int Ed Engl. 27:1988;913-922.
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0032526868
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Reasoning enantioselectivity and kinetics of seleno-subtilisin from the subtilisin template
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Häring D, Hubert B, Schüler E, Schreier P Reasoning enantioselectivity and kinetics of seleno-subtilisin from the subtilisin template. Arch Biochim Biophys. 354:1998;263-269.
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Arch Biochim Biophys
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Häring, D.1
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28
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0345055315
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Novel biocatalysts by chemical modification of known enzymes: Cross-linked microcrystals of the semisynthetic peroxidase seleno-subtilisin
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The serine protease subtilisin was crystallised, cross-linked and chemically converted into a semisynthetic peroxidase. Cross-linked crystals of seleno-subtilisin were reusable, highly stable and reduced enantioselectively racemic hydroperoxides. The first combination of cross-linked enzyme crystals and semisynthetic enzymes demonstrated the potential of chemical engineering of enzymes.
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Häring D, Schreier P Novel biocatalysts by chemical modification of known enzymes: cross-linked microcrystals of the semisynthetic peroxidase seleno-subtilisin. Angew Chem Int Ed Engl. 37:1998;2471-2473. The serine protease subtilisin was crystallised, cross-linked and chemically converted into a semisynthetic peroxidase. Cross-linked crystals of seleno-subtilisin were reusable, highly stable and reduced enantioselectively racemic hydroperoxides. The first combination of cross-linked enzyme crystals and semisynthetic enzymes demonstrated the potential of chemical engineering of enzymes.
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Angew Chem Int Ed Engl
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Häring, D.1
Schreier, P.2
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29
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33845185284
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Conversion of a protease into an acyl transferase: Selenosubtilisin
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Wu ZP, Hilvert D Conversion of a protease into an acyl transferase: selenosubtilisin. J Am Chem Soc. 111:1989;4513-4514.
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30
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0032513723
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Protein crystals as novel microporous materials
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The characteristics of cross-linked protein (including enzyme) crystals as microporous materials were studied. The crystals were applied for the first time as stationary phases in chromatography. This new aspect of protein crystals as 'organic zeolites' may lead to further novel applications.
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Vilenchik LZ, Griffith JP, St Clair NL, Navia MA, Margolin AL Protein crystals as novel microporous materials. J Am Chem Soc. 120:1998;4290-4294. The characteristics of cross-linked protein (including enzyme) crystals as microporous materials were studied. The crystals were applied for the first time as stationary phases in chromatography. This new aspect of protein crystals as 'organic zeolites' may lead to further novel applications.
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(1998)
J Am Chem Soc
, vol.120
, pp. 4290-4294
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Vilenchik, L.Z.1
Griffith, J.P.2
St Clair, N.L.3
Navia, M.A.4
Margolin, A.L.5
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