Alteration of the substrate specificity of benzoylformate decarboxylase from Pseudomonas putida by directed evolution

ChemBioChem

Volumn 4, Issue 8, 2003, Pages 721-726

  Lingen, Bettina ^a   Kolter Jung, Doris ^b   Dünkelmann, Pascal ^b   Feldmann, Ralf ^b   Grötzinger, Joachim ^c   Pohl, Martina ^a   Müller, Michael ^b  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b FORSCHUNGSZENTRUM JÜLICH   (Germany)

^c UNIVERSITY OF KIEL   (Germany)

Author keywords

Asymmetric synthesis; Directed evolution; Enzyme catalysis hydroxy ketones; Protein engineering

Indexed keywords

BACTERIAL ENZYME; BENZALDEHYDE DERIVATIVE; BENZOYLFORMATE DECARBOXYLASE; CARBOXYLYASE; KETOL; MUTANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; ASYMMETRIC SYNTHESIS; BIOCATALYST; CATALYSIS; DECARBOXYLATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; EVOLUTION; GAS CHROMATOGRAPHY; GENE MUTATION; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTON NUCLEAR MAGNETIC RESONANCE; PSEUDOMONAS PUTIDA;

BINDING SITES; CARBOXY-LYASES; CATALYSIS; EVOLUTION; KETONES; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PSEUDOMONAS PUTIDA; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0041977179     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/cbic.200200475     Document Type: Article

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