Rapid generation of incremental truncation libraries for protein engineering using α-phosphothioate nucleotides

Nucleic Acids Research

Volumn 29, Issue 4, 2021, Pages

  Lutz, Stefan ^a   Ostermeier, Marc ^a   Benkovic, Stephen J ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA PHOSPHOTHIOATE NUCLEOTIDE DERIVATIVE; DNA POLYMERASE; EXODEOXYRIBONUCLEASE III; EXONUCLEASE; NUCLEOTIDE DERIVATIVE; NUCLEOTIDE TRIPHOSPHATE DERIVATIVE; PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE; UNCLASSIFIED DRUG; HYBRID PROTEIN; PEPTIDE LIBRARY; PHOSPHOROTHIOIC ACID DERIVATIVE; TRANSFERASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; DNA DEGRADATION; ESCHERICHIA COLI; EXPERIMENTAL STUDY; HOST CELL; HYDROLYSIS; INCREMENTAL TRUNCATION LIBRARY; INTERMETHOD COMPARISON; METHODOLOGY; NONHUMAN; PEPTIDE LIBRARY; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; AMINO ACID SEQUENCE; CHEMISTRY; ENZYMOLOGY; GENE DELETION; GENETIC COMPLEMENTATION; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; BASE SEQUENCE; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; HUMANS; HYDROXYMETHYL AND FORMYL TRANSFERASES; MOLECULAR SEQUENCE DATA; ORGANOTHIOPHOSPHORUS COMPOUNDS; PEPTIDE LIBRARY; PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE DELETION;

MLCS; MLOWN;

EID: 0035866186     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/29.4.e16     Document Type: Article

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