Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation

Journal of Bacteriology

Volumn 184, Issue 2, 2002, Pages 344-349

  Canada, K A ^a   Iwashita, S ^a   Shim, H ^a   Wood, T K ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1 NAPHTHOL; ALANINE; ANTHRACENE; ETHYLENE; FLUORENE; GROUND WATER; PHENANTHRENE; TOLUENE DERIVATIVE; TOLUENE ORTHO MONOOXYGENASE; TRICHLOROETHYLENE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; BURKHOLDERIA CEPACIA; DEGRADATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; EVOLUTION; GENETIC ENGINEERING; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; STEREOCHEMISTRY;

BURKHOLDERIA CEPACIA; DICHLOROETHYLENES; DIRECTED MOLECULAR EVOLUTION; GENE LIBRARY; MIXED FUNCTION OXYGENASES; MUTAGENESIS; NAPHTHOLS; SEQUENCE HOMOLOGY, NUCLEIC ACID; SUBSTRATE SPECIFICITY; TOLUENE; TRICHLOROETHYLENE;

BURKHOLDERIA; BURKHOLDERIA CEPACIA; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036136382     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.2.344-349.2002     Document Type: Article

References (47)

1. Analysis of the gene cluster encoding toluene/o-Xylene monooxygenase from Pseudomonas stutzeri OX1
2. Effect of contaminant concentration on aerobic microbial mineralization of DCE and VC in stream-bed sediments
3. Sequence analysis of the gene cluster encoding toluene-3-monooxygenase from Pseudomonas pickettii PK01
4. Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b
5. Oxidation of trichloroethylene, 1,1-dichloroethylene, and chloroform by toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1
6. Sequencing and analysis of the Methylococcus capsulatus (Bath) soluble methane monooxygenase genes
7. Molecular evolution of an arsenate detoxification pathway by DNA shuffling
8. DNA shuffling of a family of genes from diverse species accelerates directed evolution
9. Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: Chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids
10. Crystal structure of the hydroxylase component of methane monooxygenase from Methylosinus trichosporium OB3b
11. Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo
12. Biocatalytic syntheses of aromatics from D-glucose: Renewable microbial sources of aromatic compounds
13. The parB (hok/sok) locus of plasmid RI: A general purpose plasmid stabilization system
14. BioEdit: A user-friendly biological sequence editor and analysis program for Windows 95/98/NT
15. Enzymatic dihydroxylation of aromatics in enantioselective synthesis: Expanding asymmetric methodology
16. Trichloroethylene and chloroform degradation by a recombinant pseudomonad expressing soluble methane monooxygenase from Methylosinus trichosporium OB3b
17. Nucleotide sequence analysis of genes encoding a toluene/benzene-2-monooxygenase from Pseudomonas sp. strain JS150
18. Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase
19. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
20. Monitoring trichloroethylene mineralization by Pseudomonas cepacia G4 PR1
21. Breathing with chlorinated solvents
22. Toluene monooxygenase-catalyzed epoxidation of alkenes
23. A review of immiscible fluids in the subsurface: Properties, models, characterization and remediation
24. Aerobic metabolism of trichloroethylene by a bacterial isolate
25. Purification and characterization of toluene 2-monooxygenase from Burkholderia cepacia G4
26. Complete nucleotide sequence and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600
27. Methylosinus trichosporium OB3b mutants having constitutive expression of soluble methane monooxygenase in the presence of high levels of copper
28. Changes in the regiospecificity of aromatic hydroxylation produced by active site engineering in the diiron enzyme toluene 4-monooxygenase
29. Crystal structures of the methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath): Implications for substrate gating and component interactions
30. Crystal structure of a bacterial non-haem iron hydroxylase that catalyses the biological oxidation of methane
31. Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1
32. DNA sequencing with chain-terminating inhibitors
33. Isolation and Characterization of a facultatively aerobic bacterium that reductively dehalogenates tetrachloroethene to cis-1,2-dichloroethene
34. TOM, a new aromatic degradative plasmid from Burkholderia (Pseudomonas) cepacia G4
35. Constitutive degradation of trichloroethylene by an altered bacterium in a gas-phase bioreactor
36. Rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria
37. Aerobic degradation of mixtures of chlorinated aliphatics by cloned toluene-o-xylene monooxygenase and toluene o-monooxygenase in resting cells
38. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
39. Naphthalene derivatives
40. Transformation kinetics of chlorinated ethenes by Methylosinus trichosporium OB3b and detection of unstable epoxides by on-line gas chromatography
41. Enhanced plasmid stability through post-segregational killing of plasmid-free cells
42. Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase
43. Optimization of DNA shuffling for high fidelity recombination