Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli

Structure

Volumn 9, Issue 1, 2001, Pages 1-9

  Wymer, N ^a   Buchanan, L V ^a   Henderson, D ^a   Mehta, N ^a   Botting, C H ^a   Pocivavsek, L ^a   Fierke, C A ^a   Toone, E J ^a   Naismith, J H ^a  

^a DUKE UNIVERSITY   (United States)

Author keywords

Chemical synthesis; Crystal; Enzyme catalysis; Schiff base

Indexed keywords

2 KETO 3 DEOXY 6 PHOSPHOGLUCONATE ALDOLASE; FRUCTOSE BISPHOSPHATE ALDOLASE; UNCLASSIFIED DRUG;

ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL;

ALDEHYDE-LYASES; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GENE LIBRARY; KINETICS; LYSINE; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN STRUCTURE, SECONDARY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY;

EID: 0035148643     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(00)00555-4     Document Type: Article

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