Engineering p-Hydroxyphenylpyruvate Dioxygenase to a p-Hydroxymandelate Synthase and Evidence for the Proposed Benzene Oxide Intermediate in Homogentisate Formation

Biochemistry

Volumn 43, Issue 3, 2004, Pages 663-674

  Gunsior, Michele ^a   Ravel, Jacques ^a,b   Challis, Gregory L ^a,c   Townsend, Craig A ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b J CRAIG VENTER INSTITUTE   (United States)

^c UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BENZENE; BIOASSAY; CATALYSIS; CHEMICAL MODIFICATION; CRYSTAL STRUCTURE; ENZYMES; FLUORESCENCE; IRON; METABOLISM; METABOLITES; OXIDATION; OXYGEN;

CATABOLISM; DECARBOXYLATION;

BIOCHEMISTRY;

2 OXOACID; 4 HYDROXYMANDELATE SYNTHASE; 4 HYDROXYPHENYLPYRUVATE DIOXYGENASE; AMINO ACID; BENZENE DERIVATIVE; BENZENE EPOXIDE; HOMOGENTISIC ACID; IRON; MANDELIC ACID; OXEPINONE; SYNTHETASE; UNCLASSIFIED DRUG;

AMINO ACID METABOLISM; AMINO ACID SYNTHESIS; ARTICLE; CATABOLISM; CRYSTAL STRUCTURE; DECARBOXYLATION; ENZYME ENGINEERING; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PRIORITY JOURNAL; PROTEIN FAMILY; PSEUDOMONAS FLUORESCENS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STREPTOMYCES;

4-HYDROXYPHENYLPYRUVATE DIOXYGENASE; ALKYL AND ARYL TRANSFERASES; CATALYSIS; CYCLOHEXANES; GLYCINE; HOMOGENTISIC ACID; MANDELIC ACIDS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHYLOGENY; PROTEIN ENGINEERING; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STREPTOMYCES;

PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; STREPTOMYCES; STREPTOMYCES AVERMITILIS;

EID: 1642494902     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035762w     Document Type: Article

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