Improvement of oxidative and thermostability of N-carbamyl-D-amino acid amidohydrolase by directed evolution

Protein Engineering

Volumn 15, Issue 8, 2002, Pages 689-695

  Oh, Ki Hoon ^a   Nam, Sung Hun ^a   Kim, Hak Sung ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

D amino acids; Directed evolution; N carbamyl D amino acid amidohydrolase; Oxidative stability; Thermostability

Indexed keywords

BACTERIAL DNA; HYDROGEN PEROXIDE; HYDROLASE; N CARBAMOYLASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO ACID SYNTHESIS; ARTICLE; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME STRUCTURE; GENE MUTATION; MOLECULAR EVOLUTION; MUTATIONAL ANALYSIS; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; RHIZOBIUM RADIOBACTER; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; THERMOSTABILITY; WILD TYPE;

AMIDOHYDROLASES; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; GENE LIBRARY; HEAT; HYDROGEN PEROXIDE; INDUSTRIAL MICROBIOLOGY; MODELS, MOLECULAR; OXIDATIVE STRESS; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; RHIZOBIUM RADIOBACTER; STRUCTURE-ACTIVITY RELATIONSHIP;

AGROBACTERIUM; AGROBACTERIUM SP.; AGROBACTERIUM SP. KNK712; AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS);

EID: 0036699655     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/15.8.689     Document Type: Article

References (44)

1. Aida, K.I. Chibada, I., Nakayama, K., Takinami, K. and Yamada, H. (1986) In Progress in Industrial Microbiology/Biotechnology for Amino Acid Production, Vol. 24. Kodansha, Tokyo, pp. 269-279.
2. Bradford, M.M. (1976) Anal. Biochem., 72, 248-254.
3. Buson, A., Negro, A., Grassato, L., Tagliaro, M., Fontana, A. and Nuti, M.P. (1996) FEMS Microbiol. Lett., 145, 55-62.
4. Chao, Y., Juang, T., Chern, J. and Lee, C. (1999) Biotechnol. Prog., 15, 603-607.
5. Cherry, J.R., Lamsa, M.H., Schneider, P., Vind, J., Svendsen, A., Jones, A. and Pedersen, A.H. (1999) Nature Biotechnol., 17, 379-384.
6. Declerck, N., Machius, M., Wiegand, G., Huber, R. and Gaillardin, C. (2000) J. Mol. Biol., 301, 1041-1057.
7. Gao, J., Yin, D.H., Yao, Y., Sun, H., Qin, Z., Schoneich, C., Williams, T.D. and Squier, T.C. (1998) Biophys. J., 74, 1115-1134.
8. Giordano, A., Cannio, R., La Cara, F., Bartolucci, S., Rossi, M. and Raia, C.A. (1999) Biochemistry, 38, 3043-3054.
9. Giver, L., Gershenson, A., Freskgard, P. and Arnold, F.H. (1998) Proc. Natl Acad. Sci. USA, 95, 12809-12813.
10. Grifantini, R., Pratesi, C., Galli, G. and Grandi, G. (1996) J. Biol. Chem., 271, 9326-9331.
11. Ikenaka, Y., Nanba, H., Yamada, Y., Yajima, K., Takano, M. and Takahashi, S. (1998a) Biosci. Biotechnol. Biochem., 62, 882-886.
12. Ikenaka, Y., Nanba, H., Yajima, K., Yamada, Y., Takano, M. and Takahashi, S. (1998b) Biosci. Biotechnol. Biochem., 62, 1668-1671.
13. Ikenaka, Y., Nanba, H., Yajima, K., Yamada, Y., Takano, M. and Takahashi, S. (1998c) Biosci. Biotechnol. Biochem., 62, 1672-1675.
14. Johnsen, L., Fimland, G., Eijsink, V. and Nissen-Meyer, J. (2000) Appl. Environ. Microbiol., 66, 4798-4802.
15. Ju, S., Lin, L, Chien, H.R. and Hsu, W. (2000) FEMS Microbiol. Lett., 186, 215-219.
16. Kim, G.J. and Kim, H.S. (1995) Enzyme Microb. Technol., 17, 63-67.
17. Kim, G.J., Lee, D.E. and Kim, H.S. (2000a) Appl. Environ. Microbiol., 66, 2133-2138.
18. Kim, G.J., Cheon, Y.H. and Kim, H.S. (2000b) Biotechnol. Biosci., 68, 211-217.
19. Kim, Y.H., Berry, A.H., Spencer, D.H. and Stites, W.E. (2001) Protein Eng., 14, 343-347.
20. Komsa-Penkova, R., Koynova, R., Kostov, G. and Tenchov, B. (1999) Biophys. Chem., 83, 185-195.
21. Kraulis, P.J. (1991) J. Appl. Crystallogr., 24, 946-950.
22. Kurtzman, A.L., Govindarajan, S., Vahle, K., Jones, J.T., Heinrichs, V. and Patten, P.A., (2001) Curr. Opin. Biotechnol., 12, 361-370.
23. Laemmli, U.K. (1970) Nature, 227, 680-685.
24. Morawski, B., Quan, S. and Arnold, F.H. (2001) Biotechnol. Bioeng., 76, 99-107.
25. Nakai, T. et al. (2000) Struct. Fold. Des., 8, 729-727.
26. Nanba, H., Ikenaka, Y., Yamada, Y., Yajima, K., Takano, M. and Takahashi, S. (1998) Biosci. Biotechnol. Biochem., 62, 875-881.
27. Ness, J.E., Welch, M., Giver, L., Bueno, M., Cherry, J.R., Borchert, T.V., Stemmer, W.P.C. and Minshull, J. (1999) Nature Biotechnol., 17, 893-896.
28. Ostermeier, M., Shim, J.H. and Benkovic, S.J. (1999). Nature Biotechnol., 17, 1205-1209.
29. Park, J.H., Kim, G.J. and Kim, H.S. (2000) Biotechnol. Prog., 16, 564-570.
30. Petrounia, I.P. and Arnold, F.H., (2000) Curr. Opin. Biotechnol., 11, 325-330.
31. Rietveld, A.W. and Ferreira, S.T. (1998) Biochemistry, 37, 933-937.
32. Salminen, T., Teplyakov, A., Kankare, J., Cooperman, B.S., Lahti, R. and Goldman, A. (1996) Protein Sci., 5, 1014-1025.
33. Sambrook, J. and Russell, D.W. (2001) Molecular Cloning. A Laboratory Manual. 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 13.36-13.39.
34. Serrano, L., Day, A.G. and Fersht, A.R. (1993) J. Mol. Biol., 233, 305-312.
35. Shoichet, B.K., Baase, W.A., Kuroki, R. and Mattews, B.W. (1995) Proc. Natl Acad. Sci. USA, 95, 452-456.
36. Slusarczyk, H., Felber, S., Kula, M. and Pohl, M. (2000) Eur. J. Biochem., 267, 1280-1289.
37. Stemmer, W.P.C. (1994) Nature, 370, 389-391.
38. Syldatk, C., Laufer, A., Muller, R. and Hake, H. (1990) Adv. Biochem. Eng. Biotechnol., 41, 30-51.
39. Usami, A., Ohtsu, A., Takahama, S. and Fujii, T. (1996) J. Pharm. Biomed. Anal., 14, 1133-1140.
40. Wang, W., Hsu, W., Chien, F. and Chen, C. (2001) J. Mol. Biol., 306, 252-261.
41. Wiese, A., Wilms, B., Syldatk, C., Mattes, R. and Altenbuchner, J. (2001) Appl. Microbiol. Biotechnol., 55, 750-757.
42. Wright, H.T. (1991) Protein Eng., 4, 283-294.
43. Yang, Y., Jiang, L., Zhu, L., Wu, Y. and Yang, S. (2000) J. Biotechnol., 81, 113-118.
44. Zhao, H., Giver, L., Shao, Z., Affholter, J.A. and Amold, F.H. (1998) Nature Biotechnol., 1998, 16, 258-261.