Isolation of novel ribozymes that ligate AMP-activated RNA substrates

Chemistry and Biology

Volumn 4, Issue 8, 1997, Pages 607-617

  Hager, Alicia J ^a   Szostak, Jack W ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

ATP aptamer; Catalytic RNA; In vitro selection; Ligase; Ribozyme

Indexed keywords

EID: 0031215080     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(97)90246-5     Document Type: Article

References (19)

1. Bartel, D.P. & Szostak, J.W. (1993). Isolation of new ribozymes from a large pool of random sequences. Science 261, 1411-1418.
2. Ekland, E.H. & Bartel, D.P. (1995). The secondary structure and sequence optimization of an RNA ligase ribozyme. Nucleic Acids Res. 23, 3231-3238.
3. Ekland, E.H. & Bartel, D.P. (1996). RNA-catalyzed RNA polymerization using nucleoside triphosphates. Nature 382, 373-376.
4. Green, R. & Szostak, J.W. (1992). Selection of a ribozyme that functions as a superior template in a self-copying reaction. Science 258, 1910-1915.
5. Cuenoud, B. & Szostak, J.W. (1995). A DNA metalloenzyme with DNA ligase activity. Nature 375, 611-614.
6. Hager, A.H., Pollard, J.D., Jr. & Szostak, J.W. (1996). Ribozymes: aiming at RNA replication and protein synthesis. Chem. Biol. 3, 717-725.
7. Orgel, LE. (1986). RNA catalysis and the origins of life. J. Theor. Biol. 123, 127-149.
8. White, H.B. (1976). Coenzymes as fossils of an earlier metabolic state. J. Mol. Evol. 7, 101-104.
9. White, H.B. (1982). Evolution of coenzymes and the origin of pyridine nucleotides. In The Pyridine Nucleotide Coenzymes. (Everse, J., Anderson, B. & You, K.-S., eds), pp. 1-17, Academic Press, New York.
10. Sassanfar, M. & Szostak, J.W. (1993). An RNA motif that binds ATP. Nature 364, 550-553.
11. Dieckmann, T., Suzuki, E., Nakamura, G.K. & Feigon, J. (1996). Solution structure of an ATP-binding RNA aptamer reveals a novel fold. RNA 2, 628-640.
12. Jiang, F., Kumar, R.A., Jones, R.A. & Patel, D.J. (1996). Structural basis of RNA folding and recognition in an AMP-RNA aptamer complex. Nature 382, 183-186.
13. Chu, B.C.F. & Orgel, L.E. (1984). Preparation of ligation intermediates and related polynucleotide pyrophosphates. Biochim. Biophys. Acta 782, 103-105.
14. Ekland, E.H., Szostak, J.W. & Bartel, D.P. (1995). Structurally complex and highly active ligases derived from random RNA sequences. Science 269, 364-370.
15. Lorsch, J.R., Bartel, D.P. & Szostak, J.W. (1995). Reverse transcriptase reads through a 2′-5′ linkage and a 2′-thiophosphate in a template. Nucleic Acids Res. 23, 2811-2814.
16. Lorsch, J.R. & Szostak, J.W. (1995). Kinetic and thermodynamic characterization of the reaction catalyzed by a polynucleotide kinase ribozyme. Biochemistry 34, 15315-15327.
17. Lorsch, J.R. & Szostak, J.W. (1994). In vitro evolution of new ribozymes with polynucleotide kinase activity. Nature 371, 31-36.
18. Joyce, G.F., Inoue, T. & Orgel, L.E. (1984). Non-enzymatic template-directed synthesis on RNA random copolymers. J. Mol. Biol. 176, 279-306.
19. Cadwell, R.C. & Joyce, G.F. (1992). Randomization of genes by PCR mutagenesis. PCR Methods Appl. 2, 28-33.