Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents

Applied and Environmental Microbiology

Volumn 68, Issue 4, 2002, Pages 2026-2030

  Cho, Catherine Mee Hie ^a   Mulchandani, Ashok ^a   Chen, Wilfred ^a  

^a University of California Riverside   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DEGRADATION; ESCHERICHIA COLI; GENETIC ENGINEERING; HYDROLYSIS; NEUROLOGY; PESTICIDES; SUBSTRATES; TOXIC MATERIALS;

NERVE AGENTS;

ENZYMES;

BACTERIAL ENZYME; HYDROLASE; ORGANOPHOSPHORUS COMPOUND; PARATHION METHYL; PESTICIDE;

ENZYME;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL CELL; BACTERIUM DETECTION; CATALYSIS; CELL SURFACE; CHEMICAL WARFARE; ECOSYSTEM; HYDROLYSIS; NONHUMAN; PHAGE DISPLAY; SCREENING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA;

EID: 0036208056     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.4.2026-2030.2002     Document Type: Article

References (23)

1. Three-dimensional structure of the binuclear metal center of phosphotriesterase
2. The binding of substrate analogs to phosphotriesterase
3. Microbiological and biotechnological aspects of metabolism of carbamates and organophosphates
4. Rational design of organophosphorus hydrolase for altered substrate specificities
5. Purification and properties of the phosphotriesterase from Pseudomonas diminuta
6. Inactivation of organophosphorus nerve agents by the phosphotriesterase from Pseudomonas diminuta
7. Metal-substrate interactions facilitate the catalytic activity of the bacterial phosphotriesterase
8. Stereochemical constraints on the substrate specificity of phosphotriesterase
9. Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZ alpha and pUC18 or pUC19 multiple cloning sites
10. Bimetallic binding motifs in organophosphorus hydrolase are important for catalysis and structural organization
11. Rational enzyme design: Computer modeling and site-directed mutagenesis for the modification of catalytic specificity in organophosphorus hydrolase
12. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction
13. Identification of a plasmid-borne parathion hydrolase gene from Flavobacterium sp. by Southern hybridization with opd from Pseudomonas diminuta
14. Hydrolysis of organophosphate insecticides by an immobilized-enzyme system
15. Degradation of methyl parathion by a mixed bacterial culture and a Bacillus sp. isolated from different soils
16. Designed evolution of enzymatic properties
17. Biodegradation of organophosphorus pesticides using surface-expressed organophosphorus hydrolase
18. Enzymatic hydrolysis of organophosphates: Cloning and expression of a parathion hydrolase gene from Pseudomonas diminuta
19. Cell surface display of organophosphorus hydrolase using ice nucleation protein
20. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
21. Three-dimensional structure of zinc-containing phosphotriesterase with the bound substrate analog diethyl 4-methylbenzylphosphonate
22. Augmented hydrolysis of diisopropyl fluorophosphates in engineered mutants of phosphotriesterase
23. Rationally engineered mutants of phosphotriesterase for preparative scale isolation of chiral organophosphates