-
1
-
-
3042681604
-
Protein tolerance to random amino acid change
-
H.H. Guo, J. Choe, and L.A. Loeb Protein tolerance to random amino acid change Proc Natl Acad Sci USA 101 2004 9205 9210
-
(2004)
Proc Natl Acad Sci USA
, vol.101
, pp. 9205-9210
-
-
Guo, H.H.1
Choe, J.2
Loeb, L.A.3
-
2
-
-
14144251690
-
Thermodynamic prediction of protein neutrality
-
J.D. Bloom, J.J. Silberg, C.O. Wilke, D.A. Drummond, C. Adami, and F.H. Arnold Thermodynamic prediction of protein neutrality Proc Natl Acad Sci USA 102 2005 606 611
-
(2005)
Proc Natl Acad Sci USA
, vol.102
, pp. 606-611
-
-
Bloom, J.D.1
Silberg, J.J.2
Wilke, C.O.3
Drummond, D.A.4
Adami, C.5
Arnold, F.H.6
-
3
-
-
17244380498
-
On the conservative nature of intragenic recombination
-
D.A. Drummond, J.J. Silberg, M.M. Meyer, C.O. Wilke, and F.H. Arnold On the conservative nature of intragenic recombination Proc Natl Acad Sci USA 102 2005 5380 5385
-
(2005)
Proc Natl Acad Sci USA
, vol.102
, pp. 5380-5385
-
-
Drummond, D.A.1
Silberg, J.J.2
Meyer, M.M.3
Wilke, C.O.4
Arnold, F.H.5
-
4
-
-
2342562489
-
Deciphering enzymes - Genetic selection as a probe of structure and mechanism
-
K.J. Woycechowsky, and D. Hilvert Deciphering enzymes - genetic selection as a probe of structure and mechanism Eur J Biochem 271 2004 1630 1637
-
(2004)
Eur J Biochem
, vol.271
, pp. 1630-1637
-
-
Woycechowsky, K.J.1
Hilvert, D.2
-
5
-
-
0344441707
-
Assessing the plasticity of DNA target site recognition of the Pl-Scel homing endonuclease using a bacterial two-hybrid selection system
-
F.S. Gimble, C.M. Moure, and K.L. Posey Assessing the plasticity of DNA target site recognition of the Pl-Scel homing endonuclease using a bacterial two-hybrid selection system J Mol Biol 334 2003 993 1008
-
(2003)
J Mol Biol
, vol.334
, pp. 993-1008
-
-
Gimble, F.S.1
Moure, C.M.2
Posey, K.L.3
-
6
-
-
4444306430
-
Directed evolution of enzymes for product chemistry
-
F. Valetti, and G. Gilardi Directed evolution of enzymes for product chemistry Nat Prod Rep 21 2004 490 511
-
(2004)
Nat Prod Rep
, vol.21
, pp. 490-511
-
-
Valetti, F.1
Gilardi, G.2
-
8
-
-
14844342448
-
Diversifying carotenoid biosynthetic pathways by directed evolution
-
D. Umeno, A.V. Tobias, and F.H. Arnold Diversifying carotenoid biosynthetic pathways by directed evolution Microbiol Mol Biol Rev 69 2005 51 78
-
(2005)
Microbiol Mol Biol Rev
, vol.69
, pp. 51-78
-
-
Umeno, D.1
Tobias, A.V.2
Arnold, F.H.3
-
9
-
-
0035810165
-
Functional proteins from a random-sequence library
-
A.D. Keefe, and J.W. Szostak Functional proteins from a random-sequence library Nature 410 2001 715 718
-
(2001)
Nature
, vol.410
, pp. 715-718
-
-
Keefe, A.D.1
Szostak, J.W.2
-
10
-
-
0842270116
-
Enzyme-like proteins from an unselected library of designed amino acid sequences
-
Y. Wei, and M.H. Hecht Enzyme-like proteins from an unselected library of designed amino acid sequences Protein Eng Des Sel 17 2004 67 75
-
(2004)
Protein Eng des Sel
, vol.17
, pp. 67-75
-
-
Wei, Y.1
Hecht, M.H.2
-
11
-
-
4143074011
-
Estimating the prevalence of protein sequences adopting functional enzyme folds
-
D.D. Axe Estimating the prevalence of protein sequences adopting functional enzyme folds J Mol Biol 341 2004 1295 1315
-
(2004)
J Mol Biol
, vol.341
, pp. 1295-1315
-
-
Axe, D.D.1
-
12
-
-
11244281311
-
The 'evolvability' of promiscuous protein functions
-
A. Aharoni, L. Gaidukov, O. Khersonsky, S.M. Gould, C. Roodveldt, and D.S. Tawfik The 'evolvability' of promiscuous protein functions Nat Genet 37 2005 73 76 The authors examine how new protein functions evolve by looking for mutations that improve the promiscuous activities of serum paraoxonase, bacterial phosphotriesterase and carbonic anhydrase II. The weak functions of these enzymes on a range of non-native substrates can be improved by a few mutations while the natural function remains largely unaffected.
-
(2005)
Nat Genet
, vol.37
, pp. 73-76
-
-
Aharoni, A.1
Gaidukov, L.2
Khersonsky, O.3
Gould, S.M.4
Roodveldt, C.5
Tawfik, D.S.6
-
13
-
-
4644335310
-
Directed evolution as a method to create enantioselective cyclohexanone monooxygenases for catalysis in Baeyer-Villiger reactions
-
M.T. Reetz, B. Brunner, T. Schneider, F. Schulz, C.M. Clouthier, and M.M. Kayser Directed evolution as a method to create enantioselective cyclohexanone monooxygenases for catalysis in Baeyer-Villiger reactions Angew Chem Int Ed Engl 43 2004 4075 4078 In a demonstration of the utility of full-gene random mutagenesis, the authors use directed evolution to create enantioselective cyclohexane monooxygenases that can catalyze the Baeyer-Villiger reaction with an enantioselectivity of over 90%.
-
(2004)
Angew Chem Int Ed Engl
, vol.43
, pp. 4075-4078
-
-
Reetz, M.T.1
Brunner, B.2
Schneider, T.3
Schulz, F.4
Clouthier, C.M.5
Kayser, M.M.6
-
14
-
-
10844265515
-
Random mutagenesis and selection of Escherichia coli cytosine deaminase for cancer gene therapy
-
S.D. Mahan, G.C. Ireton, C. Knoeber, B.L. Stoddard, and M.E. Black Random mutagenesis and selection of Escherichia coli cytosine deaminase for cancer gene therapy Protein Eng Des Sel 17 2004 625 633
-
(2004)
Protein Eng des Sel
, vol.17
, pp. 625-633
-
-
Mahan, S.D.1
Ireton, G.C.2
Knoeber, C.3
Stoddard, B.L.4
Black, M.E.5
-
15
-
-
21244500925
-
Directed evolution of mammalian cytochrome P450 2B1: Mutations outside of the active site enhance the metabolism of several substrates including the anticancer prodrugs cyclophosphamide and Ifosfamide
-
in press.
-
Kumar S, Chen CS, Waxman DJ, Halpert JR: Directed evolution of mammalian cytochrome P450 2B1: mutations outside of the active site enhance the metabolism of several substrates including the anticancer prodrugs cyclophosphamide and Ifosfamide. J Biol Chem 2005, in press.
-
(2005)
J Biol Chem
-
-
Kumar, S.1
Chen, C.S.2
Waxman, D.J.3
Halpert, J.R.4
-
16
-
-
0942301388
-
Selection of human cytochrome P450 1A2 mutants with enhanced catalytic activity for heterocyclic amine N-hydroxylation
-
D. Kim, and F.P. Guengerich Selection of human cytochrome P450 1A2 mutants with enhanced catalytic activity for heterocyclic amine N-hydroxylation Biochemistry 43 2004 981 988
-
(2004)
Biochemistry
, vol.43
, pp. 981-988
-
-
Kim, D.1
Guengerich, F.P.2
-
17
-
-
0041324888
-
A comparison of directed evolution approaches using the beta-glucuronidase model system
-
L.A. Rowe, M.L. Geddie, O.B. Alexander, and I. Matsumura A comparison of directed evolution approaches using the beta-glucuronidase model system J Mol Biol 332 2003 851 860
-
(2003)
J Mol Biol
, vol.332
, pp. 851-860
-
-
Rowe, L.A.1
Geddie, M.L.2
Alexander, O.B.3
Matsumura, I.4
-
18
-
-
2542525398
-
Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution
-
F.J. Ghadessy, N. Ramsay, F. Boudsocq, D. Loakes, A. Brown, S. Iwai, A. Vaisman, R. Woodgate, and P. Holliger Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution Nat Biotechnol 22 2004 755 759
-
(2004)
Nat Biotechnol
, vol.22
, pp. 755-759
-
-
Ghadessy, F.J.1
Ramsay, N.2
Boudsocq, F.3
Loakes, D.4
Brown, A.5
Iwai, S.6
Vaisman, A.7
Woodgate, R.8
Holliger, P.9
-
19
-
-
20144364220
-
Discovery of pectin-degrading enzymes and directed evolution of a novel pectate lyase for processing cotton fabric
-
A.I. Solbak, T.H. Richardson, R.T. McCann, K.A. Kline, F. Bartnek, G. Tomlinson, X. Tan, L. Parra-Gessert, G.J. Frey, and M. Podar Discovery of pectin-degrading enzymes and directed evolution of a novel pectate lyase for processing cotton fabric J Biol Chem 280 2005 9431 9438
-
(2005)
J Biol Chem
, vol.280
, pp. 9431-9438
-
-
Solbak, A.I.1
Richardson, T.H.2
McCann, R.T.3
Kline, K.A.4
Bartnek, F.5
Tomlinson, G.6
Tan, X.7
Parra-Gessert, L.8
Frey, G.J.9
Podar, M.10
-
20
-
-
5644249284
-
Directed evolution of a glycosynthase from Agrobacterium sp increases its catalytic activity dramatically and expands its substrate repertoire
-
Y.W. Kim, S.S. Lee, Warren RAJ, and S.G. Withers Directed evolution of a glycosynthase from Agrobacterium sp increases its catalytic activity dramatically and expands its substrate repertoire J Biol Chem 279 2004 42787 42793
-
(2004)
J Biol Chem
, vol.279
, pp. 42787-42793
-
-
Kim, Y.W.1
Lee, S.S.2
Raj, W.3
Withers, S.G.4
-
21
-
-
9344270529
-
Directed evolution of a glycosynthase via chemical complementation
-
H. Lin, H. Tao, and V.W. Cornish Directed evolution of a glycosynthase via chemical complementation J Am Chem Soc 126 2004 15051 15059
-
(2004)
J Am Chem Soc
, vol.126
, pp. 15051-15059
-
-
Lin, H.1
Tao, H.2
Cornish, V.W.3
-
22
-
-
3042655537
-
Computational design of a biologically active enzyme
-
M.A. Dwyer, L.L. Looger, and H.W. Hellinga Computational design of a biologically active enzyme Science 304 2004 1967 1971
-
(2004)
Science
, vol.304
, pp. 1967-1971
-
-
Dwyer, M.A.1
Looger, L.L.2
Hellinga, H.W.3
-
23
-
-
0041866683
-
Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site
-
C.M. Hill, W.S. Li, J.B. Thoden, H.M. Holden, and F.M. Raushel Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site J Am Chem Soc 125 2003 8990 8991
-
(2003)
J Am Chem Soc
, vol.125
, pp. 8990-8991
-
-
Hill, C.M.1
Li, W.S.2
Thoden, J.B.3
Holden, H.M.4
Raushel, F.M.5
-
24
-
-
0035930698
-
Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution
-
D.X. Zha, S. Wilensek, M. Hermes, K.E. Jaeger, and M.T. Reetz Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution Chem Commun 2001 2664 2665
-
(2001)
Chem Commun
, pp. 2664-2665
-
-
Zha, D.X.1
Wilensek, S.2
Hermes, M.3
Jaeger, K.E.4
Reetz, M.T.5
-
25
-
-
0042665295
-
Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression
-
Y. Koga, K. Kato, H. Nakano, and T. Yamane Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression J Mol Biol 331 2003 585 592
-
(2003)
J Mol Biol
, vol.331
, pp. 585-592
-
-
Koga, Y.1
Kato, K.2
Nakano, H.3
Yamane, T.4
-
26
-
-
12344337569
-
Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations
-
S. Park, K.L. Morley, G.P. Horsman, M. Holmquist, K. Hult, and R.J. Kazlauskas Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations Chem Biol 12 2005 45 54 The authors directly compare the effectiveness of full-gene random mutagenesis and targeted mutagenesis for improving the enantioselectivity of an esterase. They find that beneficial mutations can occur both in and distant from the active site, but that active site mutations are more likely to lead to large improvements.
-
(2005)
Chem Biol
, vol.12
, pp. 45-54
-
-
Park, S.1
Morley, K.L.2
Horsman, G.P.3
Holmquist, M.4
Hult, K.5
Kazlauskas, R.J.6
-
27
-
-
9644252999
-
Increasing the synthetic performance of penicillin acylase PAS2 by structure-inspired semi-random mutagenesis
-
E.M. Gabor, and D.B. Janssen Increasing the synthetic performance of penicillin acylase PAS2 by structure-inspired semi-random mutagenesis Protein Eng Des Sel 17 2004 571 579
-
(2004)
Protein Eng des Sel
, vol.17
, pp. 571-579
-
-
Gabor, E.M.1
Janssen, D.B.2
-
28
-
-
0037399074
-
Directed evolution of O-6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo
-
A. Juillerat, T. Gronemeyer, A. Keppler, S. Gendreizig, H. Pick, H. Vogel, and K. Johnsson Directed evolution of O-6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo Chem Biol 10 2003 313 317
-
(2003)
Chem Biol
, vol.10
, pp. 313-317
-
-
Juillerat, A.1
Gronemeyer, T.2
Keppler, A.3
Gendreizig, S.4
Pick, H.5
Vogel, H.6
Johnsson, K.7
-
29
-
-
4444326623
-
Evolution of a T7 RNA polymerase variant that transcribes 2′-O-methyl RNA
-
J. Chelliserrykattil, and A.D. Ellington Evolution of a T7 RNA polymerase variant that transcribes 2′-O-methyl RNA Nat Biotechnol 22 2004 1155 1160
-
(2004)
Nat Biotechnol
, vol.22
, pp. 1155-1160
-
-
Chelliserrykattil, J.1
Ellington, A.D.2
-
30
-
-
1242314258
-
Expanding the substrate repertoire of a DNA polymerase by directed evolution
-
M. Fa, A. Radeghieri, A.A. Henry, and F.E. Romesberg Expanding the substrate repertoire of a DNA polymerase by directed evolution J Am Chem Soc 126 2004 1748 1754
-
(2004)
J Am Chem Soc
, vol.126
, pp. 1748-1754
-
-
Fa, M.1
Radeghieri, A.2
Henry, A.A.3
Romesberg, F.E.4
-
31
-
-
1242284596
-
An engineered pathway for the formation of protein disulfide bonds
-
L. Masip, J.L. Pan, S. Haldar, J.E. Penner-Hahn, M.P. DeLisa, G. Georgiou, J.C. Bardwell, and J.F. Collet An engineered pathway for the formation of protein disulfide bonds Science 303 2004 1185 1189
-
(2004)
Science
, vol.303
, pp. 1185-1189
-
-
Masip, L.1
Pan, J.L.2
Haldar, S.3
Penner-Hahn, J.E.4
Delisa, M.P.5
Georgiou, G.6
Bardwell, J.C.7
Collet, J.F.8
-
33
-
-
5044219778
-
The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination
-
J. Xie, L. Wang, N. Wu, A. Brock, G. Spraggon, and P.G. Schultz The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination Nat Biotechnol 22 2004 1297 1301
-
(2004)
Nat Biotechnol
, vol.22
, pp. 1297-1301
-
-
Xie, J.1
Wang, L.2
Wu, N.3
Brock, A.4
Spraggon, G.5
Schultz, P.G.6
-
34
-
-
0345742616
-
A new strategy for the synthesis of glycoproteins
-
Z. Zhang, J. Gildersleeve, Y.Y. Yang, R. Xu, J.A. Loo, S. Uryu, C.H. Wong, and P.G. Schultz A new strategy for the synthesis of glycoproteins Science 303 2004 371 373
-
(2004)
Science
, vol.303
, pp. 371-373
-
-
Zhang, Z.1
Gildersleeve, J.2
Yang, Y.Y.3
Xu, R.4
Loo, J.A.5
Uryu, S.6
Wong, C.H.7
Schultz, P.G.8
-
35
-
-
7744222751
-
A genetically encoded photocaged amino acid
-
N. Wu, A. Deiters, T.A. Cropp, D. King, and P.G. Schultz A genetically encoded photocaged amino acid J Am Chem Soc 126 2004 14306 14307
-
(2004)
J Am Chem Soc
, vol.126
, pp. 14306-14307
-
-
Wu, N.1
Deiters, A.2
Cropp, T.A.3
King, D.4
Schultz, P.G.5
-
36
-
-
0346334531
-
Directed evolution of the dioxygenase complex for the synthesis of furanone flavor compounds
-
L.M. Newman, H. Garcia, T. Hudlicky, and S.A. Selifonov Directed evolution of the dioxygenase complex for the synthesis of furanone flavor compounds Tetrahedron 60 2004 729 734
-
(2004)
Tetrahedron
, vol.60
, pp. 729-734
-
-
Newman, L.M.1
Garcia, H.2
Hudlicky, T.3
Selifonov, S.A.4
-
37
-
-
3843075198
-
Biphenyl dioxygenases: Functional versatilities and directed evolution
-
K. Furukawa, H. Suenaga, and M. Goto Biphenyl dioxygenases: functional versatilities and directed evolution J Bacteriol 186 2004 5189 5196
-
(2004)
J Bacteriol
, vol.186
, pp. 5189-5196
-
-
Furukawa, K.1
Suenaga, H.2
Goto, M.3
-
38
-
-
0347635518
-
Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization
-
A. Aharoni, L. Gaidukov, S. Yagur, L. Toker, I. Silman, and D.S. Tawfik Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization Proc Natl Acad Sci USA 101 2004 482 487
-
(2004)
Proc Natl Acad Sci USA
, vol.101
, pp. 482-487
-
-
Aharoni, A.1
Gaidukov, L.2
Yagur, S.3
Toker, L.4
Silman, I.5
Tawfik, D.S.6
-
39
-
-
2342569704
-
Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes
-
M. Harel, A. Aharoni, L. Gaidukov, B. Brumshtein, O. Khersonsky, R. Meged, H. Dvir, R.B.G. Ravelli, A. McCarthy, and L. Toker Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes Nat Struct Mol Biol 11 2004 412 419
-
(2004)
Nat Struct Mol Biol
, vol.11
, pp. 412-419
-
-
Harel, M.1
Aharoni, A.2
Gaidukov, L.3
Brumshtein, B.4
Khersonsky, O.5
Meged, R.6
Dvir, H.7
Ravelli, R.B.G.8
McCarthy, A.9
Toker, L.10
-
40
-
-
2442668927
-
Discovery and directed evolution of a glyphosate tolerance gene
-
L.A. Castle, D.L. Siehl, R. Gorton, P.A. Patten, Y.H. Chen, S. Bertain, H.J. Cho, N. Duck, J. Wong, and D.L. Liu Discovery and directed evolution of a glyphosate tolerance gene Science 304 2004 1151 1154 In a remarkably successful application of directed evolution, the authors use eleven rounds of random mutagenesis and DNA shuffling to improve the activity of an enzyme that confers resistance to the herbicide glyphosate by four orders of magnitude. They demonstrate that the engineered enzyme can confer herbicide resistance on a range of hosts.
-
(2004)
Science
, vol.304
, pp. 1151-1154
-
-
Castle, L.A.1
Siehl, D.L.2
Gorton, R.3
Patten, P.A.4
Chen, Y.H.5
Bertain, S.6
Cho, H.J.7
Duck, N.8
Wong, J.9
Liu, D.L.10
-
41
-
-
14644390952
-
Construction of stabilized proteins by combinatorial consensus mutagenesis
-
N. Amin, A.D. Liu, S. Ramer, W. Aehle, D. Meijer, M. Metin, S. Wong, P. Gualfetti, and V. Schellenberger Construction of stabilized proteins by combinatorial consensus mutagenesis Protein Eng Des Sel 17 2004 787 793 The authors combine directed evolution with the technique of consensus design to stabilize a protein. Their approach involves making a library of sequences containing mutations to residues that are frequently found in an alignment of related proteins.
-
(2004)
Protein Eng des Sel
, vol.17
, pp. 787-793
-
-
Amin, N.1
Liu, A.D.2
Ramer, S.3
Aehle, W.4
Meijer, D.5
Metin, M.6
Wong, S.7
Gualfetti, P.8
Schellenberger, V.9
-
42
-
-
2942674459
-
An efficient method for creation and functional analysis of libraries of hybrid type I polyketide synthases
-
B.S. Kim, D.H. Sherman, and K.A. Reynolds An efficient method for creation and functional analysis of libraries of hybrid type I polyketide synthases Protein Eng Des Sel 17 2004 277 284
-
(2004)
Protein Eng des Sel
, vol.17
, pp. 277-284
-
-
Kim, B.S.1
Sherman, D.H.2
Reynolds, K.A.3
-
43
-
-
0141908152
-
Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplification
-
A. Ikeuchi, Y. Kawarasaki, T. Shinbata, and T. Yamane Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplification Biotechnol Prog 19 2003 1460 1467
-
(2003)
Biotechnol Prog
, vol.19
, pp. 1460-1467
-
-
Ikeuchi, A.1
Kawarasaki, Y.2
Shinbata, T.3
Yamane, T.4
-
44
-
-
0642334596
-
Enhanced crossover SCRATCHY: Construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers
-
Y. Kawarasaki, K.E. Griswold, J.D. Stevenson, T. Selzer, S.J. Benkovic, B.L. Iverson, and G. Georgiou Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers Nucleic Acids Res 31 2003 e126
-
(2003)
Nucleic Acids Res
, vol.31
, pp. 126
-
-
Kawarasaki, Y.1
Griswold, K.E.2
Stevenson, J.D.3
Selzer, T.4
Benkovic, S.J.5
Iverson, B.L.6
Georgiou, G.7
-
45
-
-
0038799745
-
General method for sequence-independent site-directed chimeragenesis
-
K. Hiraga, and F.H. Arnold General method for sequence-independent site-directed chimeragenesis J Mol Biol 330 2003 287 296
-
(2003)
J Mol Biol
, vol.330
, pp. 287-296
-
-
Hiraga, K.1
Arnold, F.H.2
-
46
-
-
0037415021
-
Assembly of designed oligonucleotides as an efficient method for gene recombination: A new tool in directed evolution
-
D. Zha, A. Eipper, and M.T. Reetz Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution ChemBioChem 4 2003 34 39
-
(2003)
ChemBioChem
, vol.4
, pp. 34-39
-
-
Zha, D.1
Eipper, A.2
Reetz, M.T.3
-
47
-
-
0038731100
-
Library analysis of SCHEMA-guided protein recombination
-
M.M. Meyer, J.J. Silberg, C.A. Voigt, J.B. Endelman, S.L. Mayo, Z.G. Wang, and F.H. Arnold Library analysis of SCHEMA-guided protein recombination Protein Sci 12 2003 1686 1693
-
(2003)
Protein Sci
, vol.12
, pp. 1686-1693
-
-
Meyer, M.M.1
Silberg, J.J.2
Voigt, C.A.3
Endelman, J.B.4
Mayo, S.L.5
Wang, Z.G.6
Arnold, F.H.7
-
48
-
-
4043109994
-
Functional evolution and structural conservation in chimeric cytochromes P450: Calibrating a structure-guided approach
-
C.R. Otey, J.J. Silberg, C.A. Voigt, J.B. Endelman, G. Bandara, and F.H. Arnold Functional evolution and structural conservation in chimeric cytochromes P450: calibrating a structure-guided approach Chem Biol 11 2004 309 318
-
(2004)
Chem Biol
, vol.11
, pp. 309-318
-
-
Otey, C.R.1
Silberg, J.J.2
Voigt, C.A.3
Endelman, J.B.4
Bandara, G.5
Arnold, F.H.6
-
51
-
-
2542577199
-
Key elements for protein foldability revealed by a combinatorial approach among similarly folded but distantly related proteins
-
S. Morimoto, and A. Tamura Key elements for protein foldability revealed by a combinatorial approach among similarly folded but distantly related proteins Biochemistry 43 2004 6596 6605
-
(2004)
Biochemistry
, vol.43
, pp. 6596-6605
-
-
Morimoto, S.1
Tamura, A.2
-
52
-
-
0141523276
-
On the structural and functional modularity of glycinamide ribonucleotide formyltransferases
-
S.G. Lee, S. Lutz, and S.J. Benkovic On the structural and functional modularity of glycinamide ribonucleotide formyltransferases Protein Sci 12 2003 2206 2214
-
(2003)
Protein Sci
, vol.12
, pp. 2206-2214
-
-
Lee, S.G.1
Lutz, S.2
Benkovic, S.J.3
-
53
-
-
2342642643
-
Directed evolution of protein enzymes using nonhomologous random recombination
-
J.A. Bittker, B.V. Le, J.M. Liu, and D.R. Liu Directed evolution of protein enzymes using nonhomologous random recombination Proc Natl Acad Sci USA 101 2004 7011 7016
-
(2004)
Proc Natl Acad Sci USA
, vol.101
, pp. 7011-7016
-
-
Bittker, J.A.1
Le, B.V.2
Liu, J.M.3
Liu, D.R.4
-
54
-
-
0346500466
-
Creation of an allosteric enzyme by domain insertion
-
G. Guntas, and M. Ostermeier Creation of an allosteric enzyme by domain insertion J Mol Biol 336 2004 263 273 The authors combine the functional properties of two different proteins by non-homologous recombination. By inserting the gene for a β-lactamase into a maltose-binding protein, they create a maltose-sensitive lactamase. This is a general approach for creating functional protein hybrids.
-
(2004)
J Mol Biol
, vol.336
, pp. 263-273
-
-
Guntas, G.1
Ostermeier, M.2
-
55
-
-
8844263869
-
A molecular switch created by in vitro recombination of nonhomologous genes
-
G. Guntas, S.F. Mitchell, and M. Ostermeier A molecular switch created by in vitro recombination of nonhomologous genes Chem Biol 11 2004 1483 1487
-
(2004)
Chem Biol
, vol.11
, pp. 1483-1487
-
-
Guntas, G.1
Mitchell, S.F.2
Ostermeier, M.3
-
56
-
-
16844379112
-
Directed evolution of the promiscuous esterase activity of carbonic anhydrase II
-
S.M. Gould, and D.S. Tawfik Directed evolution of the promiscuous esterase activity of carbonic anhydrase II Biochemistry 44 2005 5444 5452
-
(2005)
Biochemistry
, vol.44
, pp. 5444-5452
-
-
Gould, S.M.1
Tawfik, D.S.2
-
57
-
-
18744388856
-
Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity
-
N. Varadarajan, J. Gam, M.J. Olsen, G. Georgiou, and B.L. Iverson Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity Proc Natl Acad Sci USA 102 2005 6855 6860 Using a FACS-based selection-counterselection yielded a three-million-fold change in specificity for a protease cleavage site.
-
(2005)
Proc Natl Acad Sci USA
, vol.102
, pp. 6855-6860
-
-
Varadarajan, N.1
Gam, J.2
Olsen, M.J.3
Georgiou, G.4
Iverson, B.L.5
|