Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos

Applied and Environmental Microbiology

Volumn 70, Issue 8, 2004, Pages 4681-4685

  Cho, Catherine Mee Hie ^a,b   Mulchandani, Ashok ^a   Chen, Wilfred ^a  

^a University of California Riverside   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONTAMINATION; CROPS; ENZYMES; HYDROLYSIS; INSECT CONTROL; ORGANIC SOLVENTS; SUBSTRATES;

CHLORPYRIFOS; HYDROLYZED SUBSTRATES; ORGANOPHOSPHORUS HYDROLASE (OPH);

PESTICIDES;

AGRICULTURAL CHEMICAL; CHLORPYRIFOS; HYDROLASE; ORGANOPHOSPHATE; ORGANOPHOSPHORUS COMPOUND; ORGANOPHOSPHORUS HYDROLASE; PARAOXON; PARATHION METHYL; UNCLASSIFIED DRUG;

MICROBIOLOGY;

ARTICLE; DIFFUSION; ENVIRONMENTAL MANAGEMENT; ENVIRONMENTAL PROTECTION; ENZYME SUBSTRATE; ESCHERICHIA COLI; HYDROLYSIS; MICROBIAL DEGRADATION; NONHUMAN;

AMINO ACID SUBSTITUTION; ARYLDIALKYLPHOSPHATASE; CHLORPYRIFOS; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ESCHERICHIA COLI; HYDROLYSIS; INSECTICIDES; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; INSECTA; LURIA; NEGIBACTERIA;

EID: 4143066086     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.8.4681-4685.2004     Document Type: Article

References (20)

1. Bushway, R. J., and Z. Fan. 1998. Complementation of GC-AED and ELISA for the determination of diazinon and chlorpyrifos in fruits and vegetables. J. Food Prot. 61:708-712.
2. Chen-Goodspeed, M., M. A. Sogorb, F. Wu, S.-B. Hong, and F. M. Raushel. 2001. Structure determinants of the substrate and stereochemical specificity of phosphotriesterase. Biochemistry 40:1325-1331.
3. Cho, C. M.-H., A. Mulchandani, and W. Chen. 2002. Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents. Appl. Environ. Microbiol. 68:2026-2030.
4. Di Sioudi, B. D., C. E. Miller, K, Lai, J. K. Grimsley, and J. R. Wild. 1999. Rational design of organophosphorus hydrolase for altered substrate specificities. Chem.-Biol. Interact. 119-120:211-223.
5. Donarski, W. J., D. P. Dumas, D. P. Heitmeyer, V. E. Lewis, and F. M. Raushel. 1989. Structure-activity relationships in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta. Biochemistry 28:4650-4655.
6. Dumas, D. P., S. R. Caldwell, J. R. Wild, and R. M. Raushel. 1989. Purification and properties of the phosphotriesterase from Pseudomonas diminuta. J. Biol. Chem. 261:19659-19665.
7. Griffiths, A. D., and D. S. Tawfik. 2003. Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization. EMBO J. 22:24-35.
8. Hill, C. M., W.-S. Li, J. B. Thoden, H. M. Holden, and F. M. Raushel. 2003. Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site. J. Am. Chem. Soc. 125:8990-8991.
9. Hong, S. B., and F. M. Raushel. 1999. Stereochemical constraints on the substrate specificity of phosphotriesterase. Biochemistry 38:1159-1165.
10. Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
11. Koča, J., C.-G. Zhan, R. C. Rittenhouse. and R. L, Ornstein. 2001. Mobility of the active site bound paraoxon and sarin in zinc-phosphotriesterase by molecular dynamics simulation and quantum chemical calculation. J. Am. Chem. Soc. 123:817-826.
12. Lai, K. H., J. K. Grimsley, B. D. Kuhlmann, L. Scapozza, S. P. Harvey, J. J. DeFrank, J. E. Kolakowski, and J. R. Wild. 1996. Rational enzyme design: computer modeling and site-directed mutagenesis for the modification of catalytic specificity in organophosphorus hydrolase. Chimia 50:430-431.
13. Lai, K., K. I. Dave, and J. R. Wild. 1994. Bimetallic binding motifs in organophosphorus hydrolase are important for catalysis and structural organization. J. Biol. Chem. 269:16579-16584.
14. Shimazu, M., A. Mulchandani, and W. Chen. 2001. Cell surface display of organophosphorus hydrolase using ice nucleation protein. Biotechnol. Prog. 17:76-80.
15. Shimazu, M., A. Mulchandani, and W. Chen. 2001. Simultaneous degradation of organophosphorus pesticides and p-nitrophenol by a genetically engineered Moraxella sp. with surface-expressed organophosphorus hydrolase. Biotechnol. Bioeng. 76:318-324.
16. Singh, B. K., A. Walker, J. A. W. Morgan, and D. J. Wright. 2003. Effects of soil pH on the biodegradation of chlorpyrifos and isolation of a chlorpyrifos-degrading bacterium. Appl. Environ. Microbiol. 69:5198-5206.
17. Stemmer, W. P. C. 1994. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Proc. Natl. Acad. Sci. USA 91:10747-10751.
18. Watkins, L. M., J. M. Kuo, M. Chen-Goodspeed, and F. M. Raushel. 1997. A combinatorial library for the binuclear metal center of bacterial phosphotriesterase. Proteins Struct. Funct. Genet. 29:553-561.
19. Wu, F, W.-S. Li, M. Chen-Goodspeed, M. A. Sogorb, and F. M. Raushel. 2000. Rationally engineered mutants of phosphotriesterase for preparative scale isolation of chiral organophosphates. J. Am. Chem. Soc. 122:10206-10207.
20. Yang, H., P. D. Carr, S. Y. McLoughlin, J. W. Liu, I. Horne, X. Qiu, C. M. J. Jeffries, R. J. Russell, J. G. Oakeshott, and D. L. Ollis. 2003. Evolution of an organophosphate-degrading enzyme: a comparison of natural and directed evolution. Protein Eng. 6:135-145.