Laboratory Evolution of Cytochrome P450 BM-3 Monooxygenase for Organic Cosolvents

Biotechnology and Bioengineering

Volumn 85, Issue 3, 2004, Pages 351-358

  Wong, Tuck Seng ^a,b   Arnold, Frances H ^a   Schwaneberg, Ulrich ^a,b  

^a California Institute of Technology   (United States)

^b JACOBS UNIVERSITY BREMEN   (Germany)

Author keywords

Bacillus megaterium; CYP102; Directed evolution; Organic solvent resistance; P450 BM 3; Random mutagenesis

Indexed keywords

ENZYME KINETICS; HYDROXYLATION; POLYCYCLIC AROMATIC HYDROCARBONS; SOLUBILITY; SOLVENTS; SUBSTRATES;

COSOLVENTS; HETEROCYCLES;

BIOTECHNOLOGY;

ACETONE; ACETONITRILE; ALCOHOL; DIMETHYL SULFOXIDE; N,N DIMETHYLFORMAMIDE; ORGANIC SOLVENT; TETRAHYDROFURAN; UNSPECIFIC MONOOXYGENASE;

ARTICLE; BIOENGINEERING; CATALYSIS; CHEMICAL REACTION; EPOXIDATION; HYDROXYLATION; LABORATORY; SOLUBILITY;

BACILLUS MEGATERIUM; BACTERIAL PROTEINS; CATALYSIS; CYTOCHROME P-450 ENZYME SYSTEM; DIMETHYL SULFOXIDE; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVATION; ESCHERICHIA COLI; FURANS; MIXED FUNCTION OXYGENASES; MUTAGENESIS, SITE-DIRECTED; ORGANIC CHEMICALS; PROTEIN ENGINEERING; SOLUTIONS; SOLVENTS; STRUCTURE-ACTIVITY RELATIONSHIP;

BACILLUS MEGATERIUM;

EID: 0842278671     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10896     Document Type: Article

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