Directed evolution to improve the thermostability of prolyl endopeptidase

Journal of Biochemistry

Volumn 128, Issue 3, 2000, Pages 441-447

  Uchiyama, Hidefumi ^a   Inaoka, Tetsuya ^a   Ohkuma Soyejima, Toyomi ^a   Togame, Hiroko ^a   Shibanaka, Yasuhiko ^a   Yoshimoto, Tadashi ^b   Kokubo, Toshio ^a  

^a Takarazuka Research Institute   (Japan)

^b NAGASAKI UNIVERSITY   (Japan)

Author keywords

Active staining; Directed evolution; Prolyl endopeptidase; Thermostability

Indexed keywords

MUTANT PROTEIN; PROLYL ENDOPEPTIDASE;

AMINO ACID SUBSTITUTION; ARTICLE; CHRYSEOBACTERIUM MENINGOSEPTICUM; ENZYME ANALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; THERMOSTABILITY;

CHRYSEOBACTERIUM MENINGOSEPTICUM; ESCHERICHIA COLI; FLAVOBACTERIUM;

EID: 0033809270     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022772     Document Type: Article

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