Directed evolution of the surface chemistry of the reporter enzyme β- glucuronidase

Nature Biotechnology

Volumn 17, Issue 7, 1999, Pages 696-701

  Matsumura, Ichiro ^a   Wallingford, John B ^a,b   Surana, Neeraj K ^a,c   Vize, Peter D ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c WASHINGTON UNIVERSITY   (United States)

Author keywords

glucuronidase; Directed evolution; DNA shuffling; In vitro evolution; Reporter gene; Xenopus laevis

Indexed keywords

BETA GLUCURONIDASE; FORMALDEHYDE; GLUTARALDEHYDE;

AMINO ACID SUBSTITUTION; ARTICLE; CELL LINEAGE; CROSS LINKING; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE MUTATION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; SURFACE PROPERTY; THERMOSTABILITY; TISSUE FIXATION; XENOPUS;

ANIMALS; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVATION; ESCHERICHIA COLI; FEMALE; FORMALDEHYDE; GENES, BACTERIAL; GENES, REPORTER; GLUCURONIDASE; GLUTARAL; HUMANS; XENOPUS;

ESCHERICHIA COLI; PROKARYOTA; XENOPUS LAEVIS;

EID: 0033054580     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/10910     Document Type: Article

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