In vitro evolution and characterization of a ligase ribozyme adapted to acidic conditions: Effect of further rounds of evolution

Biotechnology and Bioengineering

Volumn 90, Issue 1, 2005, Pages 36-45

  Miyamoto, Yoshitaka ^a   Teramoto, Naozumi ^b   Imanishi, Yukio ^a   Ito, Yoshihiro ^c  

^a NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

^b CHIBA INSTITUTE OF TECHNOLOGY   (Japan)

^c KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Extremozyme; In vitro evolution; Ligase; Ribozyme

Indexed keywords

ACIDITY; CHARACTERIZATION; PH EFFECTS; RNA;

IN VITRO EVOLUTION; LIGASE RIBOZYME; OLIGONUCLEOTIDE BINDING;

ENZYMES;

LIGASE; OLIGONUCLEOTIDE; RIBOZYME; RNA;

ACIDITY; ADAPTATION; ARTICLE; BINDING SITE; CHEMICAL REACTION; CIRCULAR DICHROISM; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME ISOLATION; ENZYME KINETICS; ENZYME MECHANISM; GENE LIBRARY; GENE SEQUENCE; GENETIC COMPLEMENTATION; GENETIC SELECTION; IN VITRO STUDY; INDUCED MUTATION; MOLECULAR CLONING; MOLECULAR EVOLUTION; PH; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; REACTION ANALYSIS; REACTION TIME; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; DNA LIGASES; ENZYME ACTIVATION; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; POLYNUCLEOTIDE LIGASES; PROTEIN ENGINEERING; RNA, CATALYTIC; SUBSTRATE SPECIFICITY;

EID: 17444391267     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20360     Document Type: Article

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