Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

Protein Engineering

Volumn 9, Issue 1, 1996, Pages 77-83

  You, L ^a,b   Arnold, F H ^a  

^a California Institute of Technology   (United States)

^b BRISTOL MYERS SQUIBB COMPANY   (United States)

Author keywords

B.subtilis; Directed evolution; Organic solvents; Random mutagenesis; Subtilisin E; Total activity

Indexed keywords

N,N DIMETHYLFORMAMIDE; PEPTIDE; SUBTILISIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME MODIFICATION; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHUTTLE VECTOR;

EID: 0029969577     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/9.1.77     Document Type: Article

References (29)

1. Arnold,F.H. (1993) FASEB J., 7, 744-749.
2. Arnold,F.H. (1996) Chem. Eng. Sci., in press.
3. Bryan,P.N., Rollence,M.L., Pantoliano,M.W., Wood,J., Finzel,B.C., Gilliland,G.L., Howard,A.J. and Poulos,T.L. (1986) Proteins: Struct. Funct. Genet., 1, 326-334.
4. Canosi,U., Morelli,G. and Trautner,T.A. (1978) Mol. Gen. Genet., 166, 259-267.
5. Chen,K.Q. and Arnold,F.H. (1993) Proc. Natl Acad. Sci. USA, 90, 5618-5622.
6. Cunningham,B.C. and Wells,J.A. (1987) Protein Engng, 1, 319-325.
7. Dauter,Z , Betzel,C., Genov,N., Pipon,N. and Wilson,K.S. (1991) Acta Crystallogr., B47, 707-730.
8. de Vos,W.M., Venema,G., Canosi,U. and Trautner,T.A. (1981) Mol. Gen. Gener., 181, 424-433.
9. Graham,L.D., Haggett,K.D., Jennings,P.A., Le Brocque,D.S. and Whittaker,R.G. (1993) Biochemistry, 32, 6250-6258.
10. Ikemura,H., Takagi,H. and Inouye,M. (1987) J. Biol. Chem., 262, 7859-7864.
11. Joyet,P., Declerck,N. and Gailardin,C. (1992) Bio/Technology, 10, 1579-1583.
12. Leighton,T.J. and Doi,R.H. (1971) J. Biol. Chem., 246, 3189-3195.
13. Leung,D.W., Chen,E. and Goeddel,D.V. (1989) Technique, 1, 11-15.
14. Liao,H., McKenzie,T. and Hageman,R. (1986) Proc. Natl Acad. Sci. USA. 83, 576-580.
15. McKenzie,T., Hoshiro,T., Tanaka,T. and Sueka,N. (1986) Plasmid, 15, 93-103.
16. McKenzie,T., Hoshiro,T., Tanaka,T. and Sueoka,N. (1987) Plasmid, 17, 83-85.
17. Margolin,A.L., Fitzpatrick,P.A., Dubin,P.L. and Klibanov,A.M. (1991) J. Am. Chem. Soc., 113, 4693-4694.
18. Mottes,M., Grandi,G., Sgaramella,V., Canosi,U., Morelli,G. and Trautner,T.A. (1979) Mol. Gen. Genet., 174, 281-286.
19. Oliphant,A.R. and Struhl,K. (1989) Proc. Natl Acad. Sci. USA, 86, 9094-9098.
20. Park,S.-S., Wong,S.-L., Wang,L.-F. and Doi,R.H. (1989) J. Bacteriol., 6, 521-527.
21. Riva,S. and Klibanov,A.M. (1988) J. Am. Chem. Soc., 10, 3291-3295.
22. Riva,S., Chopineau,J., Kieboom,A.P.G. and Klibanov,A.M. (1988) J. Am. Chem. Soc.., 110, 584-589.
23. Saiki,R.K., Gelfand,D.H., Stoffel,S., Scharf,S.J., Higuchi,R., Horn,G.T., Mullis,K.B. and Erlich,H.A. (1988) Science, 239, 487-491.
24. Sambrook,J., Fritsch,E.F. and Maniatis,T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Siezen,R.J., De Vos,W.M., Leunissen,J.A.M. and Dijkstra,B.W. (1991) Protein Engng., 4, 719-737.
26. Stemmer,W.P.C. (1994) Nature, 370, 389-391.
27. Strausberg,S.L., Alexander,P.A., Gallagher,D.T., Gilliland,G.L., Barnett,B.L. and Bryan,P.N. (1995) Bio/Technology, 13, 669-673.
28. Wells,J.A. and Estell,D.A. (1988) Trends Biochem. Sci., 13, 291-297.
29. Wong,C.-H. and Wang,K.-T. (1991) Experientia, 47, 1123-1129.