Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

Protein Engineering

Volumn 9, Issue 1, 1996, Pages 77-83

  You, L ^a,b   Arnold, F H ^a  

^a California Institute of Technology ^*  (United States)

^b BRISTOL MYERS SQUIBB COMPANY   (United States)

Author keywords

B.subtilis; Directed evolution; Organic solvents; Random mutagenesis; Subtilisin E; Total activity

Indexed keywords

N,N DIMETHYLFORMAMIDE; PEPTIDE; SUBTILISIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME MODIFICATION; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHUTTLE VECTOR;

EID: 0029969577     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/9.1.77     Document Type: Article

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