Directed evolution of a recombinase for improved genomic integration at a native human sequence

Nucleic Acids Research

Volumn 29, Issue 24, 2001, Pages 5044-5051

  Sclimenti, Christopher R ^a   Thyagarajan, Bhaskar ^a   Calos, Michele P ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRASE; RECOMBINASE;

ARTICLE; BACTERIOPHAGE; CHROMOSOME 8; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; DNA INTEGRATION; DNA MODIFICATION; DNA SCREENING; DNA SEQUENCE; ESCHERICHIA COLI; EUKARYOTIC CELL; GENOME; HUMAN; HUMAN CELL; MAMMAL; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL;

ESCHERICHIA COLI; EUKARYOTA; MAMMALIA; NEGIBACTERIA;

EID: 0035945238     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/29.24.5044     Document Type: Article

References (29)

1. Mammalian genomes contain active recombinase recognition sites
2. Analysis of the integration function of the Streptomycete bacteriophage ΦC31
3. Structural analysis of the actinophage ΦC31 attachment site
4. In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family
5. A phage integrase directs efficient site-specific integration in human cells
6. Site-specific genomic integration in mammalian cells mediated by phage φC31 integrase
7. Directed evolution of a paranitrobenzyl esterase for aqueous-organic solvents
8. Protein evolution by molecular breeding
9. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
10. Rapid evolution of a protein in vitro by DNA shuffling
11. DNA shuffling of a family of genes from diverse species accelerates directed evolution
12. New cloning vectors with temperature-sensitive replication
13. Promoters of Escherichia coli: A hierarchy of in vivo strength indicates alternate structures
14. Construction of lacIts and lacIqts expression plasmids and evaluation of the thermosensitive lac repressor
15. A novel method for real time quantitative RT-PCR
16. Real time quantitative PCR
17. Crystal-structure of the site-specific recombinase gamma-delta resolvase complexed with a 34 bp cleavage site
18. The tyrosine-6 hydroxyl of gamma delta resolvase is not required for the DNA cleavage and rejoining reactions
19. Catalysis by site-specific recombinases
20. Exploring the DNA-binding specificities of zinc fingers with DNA microarrays
21. Towards the design of rare cutting restriction endonucleases: Using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude
22. Improved properties of FLP recombinase evolved by cycling mutagenesis
23. Cre mutants with altered DNA binding properties
24. Identifying determinants of recombination specificity: Construction and characterization of mutant bacteriophage integrases
25. Identifying determinants of recombination specificity: Construction and characterization of chimeric bacteriophage integrases
26. Site-specific recombination in mammalian cells catalyzed by γδ resolvase mutants: Implications for the topology of episomal DNA
27. Altering the DNA-binding specificity of Mu transposase in vitro
28. Molecular genetic analysis of transposase-end DNA sequence recognition: Cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn5 transposase