Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution

Nature Biotechnology

Volumn 22, Issue 6, 2004, Pages 755-759

  Ghadessy, Farid J ^a   Ramsay, Nicola ^a   Boudsocq, François ^b   Loakes, David ^a   Brown, Anthony ^c   Iwai, Shigenori ^d   Vaisman, Alexandra ^b   Woodgate, Roger ^b   Holliger, Philipp ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^c BioRobotics Ltd   (United Kingdom)

^d UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC CONVERTERS; DIMERIZATION; FLUORESCENCE; SIGNAL DISTORTION; SUBSTRATES;

NUCLEOTIDES; POLYMERASES; TRIPHOSPHATE;

DNA;

DNA POLYMERASE; NUCLEOTIDE DERIVATIVE; NUCLEOTIDE TRIPHOSPHATE; TAQ POLYMERASE; UNCLASSIFIED DRUG;

ARTICLE; DIRECTED MOLECULAR EVOLUTION; DNA HYBRIDIZATION; ENZYME SPECIFICITY; GENE DUPLICATION; MOLECULAR RECOGNITION; NUCLEIC ACID BASE SUBSTITUTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRUCTURAL GENE;

BASE PAIR MISMATCH; BIOTIN; DEOXYADENINE NUCLEOTIDES; DEOXYGUANINE NUCLEOTIDES; DEOXYRIBOSE; DEOXYURACIL NUCLEOTIDES; DIRECTED MOLECULAR EVOLUTION; DNA; DNA MUTATIONAL ANALYSIS; DNA PROBES; FLUORESCEIN-5-ISOTHIOCYANATE; INDOLES; KINETICS; MICROARRAY ANALYSIS; MODELS, MOLECULAR; MUTATION; POINT MUTATION; POLYMERASE CHAIN REACTION; PYRIMIDINE DIMERS; RHODAMINES; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; TAQ POLYMERASE; THIONUCLEOTIDES;

EID: 2542525398     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt974     Document Type: Article

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