Alteration of substrate specificity of aspartase by directed evolution

Biomolecular Engineering

Volumn 22, Issue 1-3, 2005, Pages 95-101

  Asano, Yasuhisa ^a   Kira, Ikuo ^a,b   Yokozeki, Kenzo ^b  

^a TOYAMA PREFECTURAL UNIVERSITY   (Japan)

^b AJINOMOTO CO INC   (Japan)

Author keywords

Alteration of substrate specificity; Aspartase; Directed evolution

Indexed keywords

AMINATION; AMINO ACIDS; CATALYSIS; ESCHERICHIA COLI; GENES; MUTAGENESIS; PH;

ASPARTASE; MUTANT LIBRARY; MUTATED ENZYMES; RANDOM MUTAGENESIS;

ENZYMES;

ASPARTATE AMMONIA LYASE; ASPARTIC ACID ALPHA AMIDE; ASPARTIC ACID DERIVATIVE; ENZYME VARIANT; FUMARIC ACID DERIVATIVE; FUMARIC ACID MONOAMIDE; UNCLASSIFIED DRUG;

ACIDITY; ALKALINITY; ARTICLE; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; ENZYME MECHANISM; ENZYME SPECIFICITY; ERROR; GENETIC ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 18044384249     PISSN: 13890344     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bioeng.2004.12.002     Document Type: Article

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