Biofortified Crops Generated by Breeding, Agronomy, and Transgenic Approaches Are Improving Lives of Millions of People around the World

Frontiers in Nutrition

Volumn 5, Issue , 2018, Pages

  Garg, Monika ^a   Sharma, Natasha ^a   Sharma, Saloni ^a   Kapoor, Payal ^a   Kumar, Aman ^a   Chunduri, Venkatesh ^a   Arora, Priya ^a  

^a NATIONAL AGRI FOOD BIOTECHNOLOGY INSTITUTE   (India)

Author keywords

agronomic; biofortification; breeding; malnutrition; transgenic

Indexed keywords

EID: 85054489813     PISSN: None     EISSN: 2296861X     Source Type: Journal    
DOI: 10.3389/fnut.2018.00012     Document Type: Review

References (325)

1. McGuire S. FAO, IFAD, and WFP. The state of food insecurity in the world 2015: meeting the 2015 international hunger targets: taking stock of uneven progress. Rome: FAO. Adv Nutr (2015) 6(5):623–4.10.3945/an.115.009936
2. Hodge J. Hidden hunger: approaches to tackling micronutrient deficiencies. In: Gillespie S, Hodge J, Yosef S, Pandya-Lorch R, editors. Nourishing Millions: Stories of Change in Nutrition. Washington: International Food Policy Research Institute (IFPRI) (2016). p. 35–43.
3. Muthayya A, Rah JH, Sugimoto JD, Roos FF, Kraemer K, Black RE. The global hidden hunger indices and maps: an advocacy tool for action. PLoS One (2013) 8(6):e67860.10.1371/journal.pone.006786023776712
4. Gould J. Nutrition: a world of insecurity. Nat Outlook (2017) 544:S7.10.1038/544S6a
5. Khush GS, Lee S, Cho JI, Jeon JS. Biofortification of crops for reducing malnutrition. Plant Biotechnol Rep (2012) 6:195–202.10.1007/s11816-012-0216-5
6. Gilani GS, Nasim A. Impact of foods nutritionally enhanced through biotechnology in alleviating malnutrition in developing countries. J AOAC Int (2007) 90(5):1440–4.17955991
7. Perez-Massot E, Banakar R, Gomez-Galera S, Zorrilla-Lopez U, Sanahuja G, Arjo G, et al. The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints. Genes Nutr (2013) 8(1):29–41.10.1007/s12263-012-0315-522926437
8. Bouis HE. Economics of enhanced micronutrient density in food staples. Field Crops Res (1999) 60:165–73.10.1016/S0378-4290(98)00138-5
9. Nestel P, Bouis HE, Meenakshi JV, Pfeiffer W. Biofortification of staple food crops. J Nutr (2006) 136:1064–7.10.1093/jn/136.4.106416549478
10. Pfeiffer WH, McClafferty B. HarvestPlus: breeding crops for better nutrition. Crop Sci (2007) 47:S88–100.10.2135/cropsci2007.09.0020IPBS
11. Qaim M, Stein AJ, Meenakshi JV. Economics of biofortification. Agric Econ (2007) 37:119–33.10.1111/j.1574-0862.2007.00239.x
12. Hirschi KD. Nutrient biofortification of food crops. Annu Rev Nutr (2009) 29:401–21.10.1146/annurev-nutr-080508-14114319400753
13. Meenakshi JV, Johnson NL, Manyong VM, DeGroote H, Javelosa J, Yanggen DR, et al. How cost-effective is biofortification in combating micronutrient malnutrition? An ex ante assessment. World Dev (2010) 38(1):64–75.10.1016/j.worlddev.2009.03.014
14. Hefferon KL. Can biofortified crops help attain food security? Curr Mol Biol Rep (2016) 2(4):180–5.10.1007/s40610-016-0048-0
15. Bazuin S, Azadi H, Witlox F. Application of GM crops in Sub-Saharan Africa: lessons learned from green revolution. Biotechnol Adv (2011) 29:908–12.10.1016/j.biotechadv.2011.07.01121813087
16. Das JK, Kumar R, Salam RA, Bhutta ZA. Systematic review of zinc fortification trials. Ann Nutr Metab (2013) 62(1):44–56.10.1159/00034826223689112
17. Bouis HE. Enrichment of food staples through plant breeding: a new strategy for fighting micronutrient malnutrition. Nutrition (2000) 16:701–4.10.1016/S0899-9007(00)00266-5
18. Prashanth L, Kattapagari KK, Chitturi RT, Baddam VR, Prasad LK. A review on role of essential trace elements in health and disease. J NTR Univ Health Sci (2015) 4:75–85.10.4103/2277-8632.158577
19. White J, Broadley MR. Biofortifying crops with essential mineral elements. Trends Plant Sci (2005) 10:586–93.10.1016/j.tplants.2005.10.00116271501
20. Welch RM, Graham RD. Breeding for micronutrients in staple food crops from a human nutrition perspective. J Exp Bot (2004) 55:353–64.10.1093/jxb/erh06414739261
21. Graham RD, Welch RM, Bouis HE. Addressing micronutrient malnutrition through enhancing the nutritional quality of staple foods: principles, perspectives and knowledge gaps. Adv Agron (2001) 70:77–142.10.1016/S0065-2113(01)70004-1
22. McGuire J. Addressing micronutrient malnutrition. SCN News (1993) 9:1–10.
23. Schneeman BO. Linking agricultural production and human nutrition. J Sci Food Agri (2001) 81:3–9.10.1002/1097-0010(20010101)81:1<3::AID-JSFA743>3.0.CO;2-Q
24. Chizuru N, Ricardo U, Shiriki K, Prakash S. The joint WHO/FAO expert consultation on diet, nutrition and the prevention of chronic diseases: process, product and policy implications. Public Health Nutr (2003) 7(1a):245–50.10.1079/PHN2003592
25. Branca F, Ferrari M. Impact of micronutrient deficiencies on growth: the stunting syndrome. Ann Nutr Metab (2002) 46:8–17.10.1159/00006639712428076
26. Golden MHN. The nature of nutritional deficiencies in relation to growth failure and poverty. Acta Paediatr Scand (1991) 374:95–110.10.1111/j.1651-2227.1991.tb12012.x
27. Grantham-McGregor SM, Ani CC. The role of micronutrients in psychomotor sad cognitive development. Br Med Bull (1999) 55:511–27.10.1258/0007142991902583
28. Ramakrishnan U, Manjrekar R, Rivera J, Gonzales-Cossio T, Martorell R. Micronutrients and pregnancy outcome: a review of the literature. Nutr Res (1999) 19:103–59.10.1016/S0271-5317(98)00178-X
29. Caballero B. Global patterns of child health: the role of nutrition. Ann Nutr Metab (2002) 46:3–7.10.1159/000066400
30. Stevens GA, Finucane MM, De-Regil L, Paciorek CJ, Flaxman SR, Branca F, et al. Global, regional, and national trends in haemoglobin concentration and prevalence of total and severe anaemia in children and pregnant and non-pregnant women for 1995–2011: a systematic analysis of population-representative data. Lancet Glob Health (2013) 1(1):e16–25.10.1016/S2214-109X(13)70001-9
31. Brotanek JM, Halterman JS, Auinger P, Flores G, Weitzman M. Iron deficiency, prolonged bottle-feeding, and racial/ethnic disparities in young children. Arch Pediatr Adolesc Med (2005) 159:1038–42.10.1001/archpedi.159.11.103816275794
32. Zhu C, Naqvi S, Gomez-Galera S, Pelacho AM, Capell T, Christou P. Transgenic strategies for the nutritional enhancement of plants. Trends Plant Sci (2007) 12:548–55.10.1016/j.tplants.2007.09.00718006362
33. Welch RM, Graham RD. A new paradigm for world agriculture: meeting human needs-productive, sustainable, nutritious. Field Crops Res (1999) 60:1–10.10.1016/S0378-4290(98)00129-4
34. Saltzman A, Birol E, Bouis HE, Boy E, De Moura FF, Islam Y, et al. Biofortification: progress toward a more nourishing future. Glob Food Secur (2014) 2(1):9–17.10.1016/j.gfs.2012.12.003
35. Brinch-Pedersen H, Borg S, Tauris B, Holm PB. Molecular genetic approaches to increasing mineral availability and vitamin content of cereals. J Cereal Sci (2007) 46:308–26.10.1016/j.jcs.2007.02.004
36. Christou P, Twyman RM. The potential of genetically enhanced plants to address food insecurity. Nutr Res Rev (2004) 17:23–42.10.1079/NRR20037319079913
37. Newell-McGloughlin M. Nutritionally improved agricultural crops. Plant Physiol (2008) 147:939–53.10.1104/pp.108.121947
38. Shewmaker CK, Sheehu JA, Daley M, Colburn S, Ke DY. Seed-specific overexpression of phytoene synthase: increase in carotenoids and metabolic effects. Plant J (1999) 20:41–412.10.1046/j.1365-313x.1999.00611.x
39. Agrawal PK, Kohli A, Twyman RM, Christou P. Transformation of plants with multiple cassettes generates simple transgene integration patterns and high expression levels. Mol Breed (2005) 16:247–60.10.1007/s11032-005-0239-5
40. Yang SH, Moran DL, Jia HW, Bicar EH, Lee M, Scott MP. Expression of a synthetic porcine alpha-lactalbumin gene in the kernels of transgenic maize. Transgenic Res (2002) 11:11–20.10.1023/A:101399612912511874099
41. Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, Beyer P, et al. Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoids-free) rice endosperm. Science (2000) 287:303–5.10.1126/science.287.5451.303
42. Beyer P, Al-Babili S, Ye X, Lucca P, Schaub P, Welsch R, et al. Golden rice: introducing the β-carotene biosynthesis pathway into rice endosperm by genetic engineering to defeat vitamin A deficiency. J Nutr (2002) 132(3):506S–10S.10.1093/jn/132.3.506S
43. Datta K, Baisakh N, Oliva N, Torrizo L, Abrigo E, Tan J, et al. Bioengineered ‘golden’ Indica rice cultivars with beta-carotene metabolism in the endosperm with hygromycin and mannose selection systems. Plant Biotechnol J (2003) 1:81–90.10.1046/j.1467-7652.2003.00015.x17147745
44. Paine JA, Shipton CA, Chaggar S, Howells RM, Kennedy MJ, Vernon G, et al. Improving the nutritional value of golden rice through increased pro-vitamin A content. Nat Biotechnol (2005) 23:482–7.10.1038/nbt108215793573
45. Burkhardt PK, Beyer P, Wuenn J, Kloeti A, Armstrong GA, Schledz M, et al. Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. Plant J (1997) 11:1071–8.10.1046/j.1365-313X.1997.11051071.x9193076
46. Storozhenko S, De Brouwer V, Volckaert M, Navarrete O, Blancquaert D, Zhang GF, et al. Folate fortification of rice by metabolic engineering. Nat Biotechnol (2007) 25(11):1277–9.10.1038/nbt135117934451
47. Blancquaert D, Van daele J, Strobbe S, Kiekens F, Storozhenko S, De Steur H, et al. Improving folate (vitamin B9) stability in biofortified rice through metabolic engineering. Nat Biotechnol (2015) 33:1076–8.10.1038/nbt.335826389575
48. Takahashi M, Nakanishi H, Kawasaki S, Nishizawa NK, Mori S. Enhanced tolerance of rice to low iron availability in alkaline soils using barley nicotianamine aminotransferase genes. Nat Biotechnol (2001) 19:466–9.10.1038/8814311329018
49. Lee S, An G. Over-expression of OsIRT1 leads to increased iron and zinc accumulations in rice. Plant Cell Environ (2009) 32:408–16.10.1111/j.1365-3040.2009.01935.x19183299
50. Zheng L, Cheng Z, Ai C, Jiang X, Bei X, Zheng Y, et al. Nicotianamine, a novel enhancer of rice iron bioavailability to humans. PLoS One (2010) 5(4):e10190.10.1371/journal.pone.001019020419136
51. Lee S, Kim YS, Jeon US, Kim YK, Schjoerring JK, An G. Activation of rice nicotinamine synthase 2 (OsNAS2) enhances iron availability for biofortification. Mol Cell (2012) 33:269–75.10.1007/s10059-012-2231-3
52. Trijatmiko K, Duenas C, Tsakirpaloglou N, Torrizo L, Arines FM, Adeva C, et al. Biofortified indica rice attains iron and zinc nutrition dietary targets in the field. Sci Rep (2016) 6:19792.10.1038/srep1979226806528
53. Goto F, Yoshihara T, Shigemoto N, Toki S, Takaiwa F. Iron fortification of rice seed by the soybean ferritin gene. Nat Biotechnol (1999) 17:282–6.10.1038/702910096297
54. Vasconcelos M, Datta K, Oliva N, Khalekuzzaman M, Torrizo L, Krishnan S, et al. Enhanced iron and zinc accumulation in transgenic rice with the ferritin gene. Plant Sci (2003) 164:371–8.10.1016/S0168-9452(02)00421-1
55. Lucca P, Hurrell R, Potrykus I. Fighting iron deficiency anemia with iron-rich rice. J Am Coll Nutr (2002) 21:184S–90S.10.1080/07315724.2002.1071926412071303
56. Wirth J, Poletti S, Aeschlimann B, Yakandawala N, Drosse B, Osorio S, et al. Rice endosperm iron biofortification by targeted and synergistic action of nicotianamine synthase and ferritin. Plant Biotechnol J (2009) 7:631–44.10.1111/j.1467-7652.2009.00430.x19702755
57. Masuda H, Ishimaru Y, Aung MS, Kobayashi T, Kakei Y, Takahashi M, et al. Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition. Sci Rep (2012) 2:534.10.1038/srep0054322848789
58. Masuda H, Kobayashi T, Ishimaru Y, Takahashi M, Aung MS, Nakanishi H, et al. Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene. Front Plant Sci (2013) 4:132.10.3389/fpls.2013.0013223675379
59. Hurrell R, Egli I. Iron bioavailability and dietary reference values. Am J Clin Nutr (2010) 91:1461S–7S.10.3945/ajcn.2010.28674F20200263
60. Masuda H, Suzuki M, Morikawa KC, Kobayashi T, Nakanishi H, Takahashi M, et al. Increase in iron and zinc concentrations in rice grains via the introduction of barley genes involved in phytosiderophore synthesis. Rice (2008) 1:100–8.10.1007/s12284-008-9007-6
61. Zheng A, Sumi K, Tanaka K, Murai N. The bean seed storage protein β-phaseolin is synthesized, processed and accumulated in the vacuolar type-II protein bodies of transgenic rice endosperm. Plant Physiol (1995) 109:777–86.10.1104/pp.109.3.777
62. Sindhu AS, Zheng Z, Murai N. The pea seed storage protein legumin was synthesized, processed, and accumulated stably in transgenic rice endosperm. Plant Sci (1997) 130:189–96.10.1016/S0168-9452(97)00219-7
63. Lee TTT, Wang MMC, Hou RCW, Chen LJ, Su RC, Wang CS, et al. Enhanced methionine and cysteine levels in transgenic rice seeds by the accumulation of sesame 2S albumin. Biosci Biotechnol Biochem (2003) 67:1699–705.10.1271/bbb.67.169912951502
64. Katsube T, Kurisaka N, Ogawa M, Maruyama N, Ohtsuka R, Utsumi S, et al. Accumulation of soybean glycinin and its assembly with the glutelins in rice. Plant Physiol (1999) 120:1063–73.10.1104/pp.120.4.106310444090
65. Yang QQ, Zhang CQ, Chan ML, Zhao DS, Chen JZ, Wang Q, et al. Biofortification of rice with the essential amino acid lysine: molecular characterization, nutritional evaluation, and field performance. J Exp Bot (2016) 67(14):4285–96.10.1093/jxb/erw20927252467
66. Lee SI, Kim HU, Lee YH, Suh SC, Lim YP, Lee HY, et al. Constitutive and seed-specific expression of a maize lysine-feedback-insensitive dihydrodipicolinate synthase gene leads to increased free lysine levels in rice seeds. Mol Breed (2001) 8:75–84.10.1023/A:1011977219926
67. Wakasa K, Hasegawa H, Nemoto H, Matsuda F, Miyazawa H, Tozawa Y, et al. High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. J Exp Bot (2006) 57:3069–78.10.1093/jxb/erl06816908506
68. Zhou Y, Cai H, Xiao J, Li X, Zhang Q, Lian X. Over-expression of aspartate aminotransferase genes in rice resulted in altered nitrogen metabolism and increased amino acid content in seeds. Theor Appl Genet (2009) 118:1381–90.10.1007/s00122-009-0988-319259642
69. Anai T, Koga M, Tanaka H, Kinoshita T, Rahman SM, Takagi Y. Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene. Plant Cell Rep (2003) 21(10):988–92.10.1007/s00299-003-0609-612835909
70. Shin YM, Park HJ, Yim SD, Baek NI, Lee CH, An G, et al. Transgenic rice lines expressing maize C1 and R-S regulatory genes produce various flavonoids in the endosperm. Plant Biotechnol J (2006) 4:303–15.10.1111/j.1467-7652.2006.00182.x17147636
71. Ogo Y, Ozawa K, Ishimaru T, Murayama T, Takaiwa F. Transgenic rice seed synthesizing diverse flavonoids at high levels: a new platform for flavonoid production with associated health benefits. Plant Biotechnol J (2013) 11:734–46.10.1111/pbi.1206423551455
72. Liu Q, Wang Z, Chen X, Cai X, Tang S, Yu H, et al. Stable inheritance of the antisense waxy gene in transgenic rice with reduced amylose level and improved quality. Transgenic Res (2003) 12(1):71–82.10.1023/A:102214882401812650526
73. Itoh K, Ozaki H, Okada K, Hori H, Takeda Y, Mitsui T. Introduction of Wx transgene into rice wx mutants leads to both high- and low-amylose rice. Plant Cell Physiol (2003) 44(5):473–80.10.1093/pcp/pcg06812773633
74. Wei C, Zhang J, Chen Y, Zhou W, Xu B, Wang Y, et al. Physicochemical properties and development of wheat large and small starch granules during endosperm development. Acta Physiol Plant (2010) 32:905–16.10.1007/s11738-010-0478-x
75. Nandi S, Suzuki YA, Huang J, Yalda D, Pham P, Wu L, et al. Expression of human lactoferrin in transgenic rice grains for the application in infant formula. Plant Sci (2002) 163:713–22.10.1016/S0168-9452(02)00165-6
76. Wang C, Zeng J, Li Y, Hu W, Chen L, Miao Y, et al. Enrichment of provitamin A content in wheat (Triticum aestivum L.) by introduction of the bacterial carotenoid biosynthetic genes CrtB and CrtI. J Exp Bot (2014) 65(9):2545–56.10.1093/jxb/eru13824692648
77. Cong L, Wang C, Chen L, Liu H, Yang G, He G. Expression of phytoene synthase1 and carotene desaturase crtI genes result in an increase in the total carotenoids content in transgenic elite wheat (Triticum aestivum L.). J Agric Food Chem (2009) 57(18):8652–60.10.1021/jf901221819694433
78. Xiaoyan S, Yan Z, Shubin W. Improvement Fe content of wheat (Triticum aestivum) grain by soybean ferritin expression cassette without vector backbone sequence. J Agric Biotechnol (2012) 20:766–73.
79. Borg S, Brinch-Pedersen H, Tauris B, Madsen LH, Darbani B, Noeparvar S, et al. Wheat ferritins: improving the iron content of the wheat grain. J Cereal Sci (2012) 56:204–13.10.1016/j.jcs.2012.03.005
80. Brinch-Pederson H, Olesen A, Rasmussen SK, Holm PB. Generation of transgenic wheat (Triticum aestivum L.) for constitutive accumulation of an Aspergillus phytase. Mol Breed (2000) 6:195–206.10.1023/A:1009690730620
81. Bhati KK, Alok A, Kumar A, Kaur J, Tiwari S, Pandey AK. Silencing of ABCC13 transporter in wheat reveals its involvement in grain development, phytic acid accumulation and lateral root formation. J Exp Bot (2016) 67(14):4379–89.10.1093/jxb/erw22427342224
82. Tamas C, Kisgyorgy BN, Rakszegi M, Wilkinson MD, Yang MS, Lang L, et al. Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality. Plant Cell Rep (2009) 28(7):1085–94.10.1007/s00299-009-0716-019466426
83. Doshi KM, Eudes F, Laroche A, Gaudet D. Transient embryo specific expression of anthocyanin in wheat. In Vitro Cell Dev Biol Plant (2006) 42:432–8.10.1079/IVP2006778
84. Sestili F, Janni M, Doherty A, Botticella E, D’Ovidio R, Masci S, et al. Increasing the amylose content of durum wheat through silencing of the SBEIIa genes. BMC Plant Biol (2010) 10:144.10.1186/1471-2229-10-14420626919
85. Aluru M, Xu Y, Guo R, Wang Z, Li S, White W, et al. Generation of transgenic maize with enhanced provitamin A content. J Exp Bot (2008) 59(13):3551–62.10.1093/jxb/ern21218723758
86. Decourcelle M, Perez-Fons L, Baulande S, Steiger S, Couvelard L, Hem S, et al. Combined transcript, proteome, and metabolite analysis of transgenic maize seeds engineered for enhanced carotenoid synthesis reveals pleotropic effects in core metabolism. J Exp Bot (2015) 66(11):3141–50.10.1093/jxb/erv12025796085
87. Cahoon EB, Hall SE, Ripp KG, Ganzke TS, Hitz WD, Coughlan SJ. Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content. Nat Biotechnol (2003) 21:1082–7.10.1038/nbt85312897790
88. Levine M, Dhariwal KR, Welch RW, Wang Y, Park JB. Determination of optimal vitamin C requirements in humans. Am J Clin Nutr (1995) 62:1347S–56S.10.1093/ajcn/62.6.1347S7495230
89. Chen Z, Young TE, Ling J, Chang SC, Gallie DR. Increasing vitamin C content of plants through enhanced ascorbate recycling. Proc Natl Acad Sci U S A (2003) 100:3525–30.10.1073/pnas.063517610012624189
90. Naqvi S, Zhu C, Farre G, Ramessar K, Bassie L, Breitenbach J, et al. Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways. Proc Natl Acad Sci U S A (2009) 106(19):7762–7.10.1073/pnas.090141210619416835
91. Drakakaki G, Marcel S, Glahn RP, Lund EK, Pariagh S, Fischer R, et al. Endosperm-specific co-expression of recombinant soybean ferritin and Aspergillus phytase in maize results in significant increases in the levels of bioavailable iron. Plant Mol Biol (2005) 59(6):869–80.10.1007/s11103-005-1537-316307363
92. Aluru MR, Rodermel SR, Reddy MB. Genetic modification of low phytic acid 1-1 maize to enhance iron content and bioavailability. J Agric Food Chem (2011) 59(24):12954–62.10.1021/jf203485a22088162
93. Chen R, Xue G, Chen P, Yao B, Yang W, Ma Q, et al. Transgenic maize plants expressing a fungal phytase gene. Transgenic Res (2008) 17(4):633–43.10.1007/s11248-007-9138-317932782
94. Shi J, Wang H, Schellin K, Li B, Faller M, Stoop JM, et al. Embryo-specific silencing of a transporter reduces phytic acid content of maize and soybean seeds. Nat Biotechnol (2007) 25(8):930–7.10.1038/nbt132217676037
95. Yu J, Peng P, Zhang X, Zhao Q, Zhu D, Sun X, et al. Seed-specific expression of the lysine-rich protein gene sb401 significantly increases both lysine and total protein content in maize seeds. Food Nutr Bull (2005) 26(4):427–31.10.1177/15648265050264S31116465991
96. Tang M, He X, Luo Y, Ma L, Tang X, Huang K. Nutritional assessment of transgenic lysine-rich maize compared with conventional quality protein maize. J Sci Food Agric (2013) 93:1049–54.10.1002/jsfa.584523400871
97. Frizzi A, Huang S, Gilbertson LA, Armstrong TA, Luethy MH, Malvar TM. Modifying lysine biosynthesis and catabolism in corn with a single bifunctional expression/silencing transgene cassette. Plant Biotechnol J (2008) 6(1):13–21.10.1111/j.1467-7652.2007.00290.x17725550
98. Huang S, Frizzi A, Florida CA, Kruger DE, Luethy MH. High lysine and high tryptophan transgenic maize resulting from the reduction of both 19- and 22-kD alpha-zeins. Plant Mol Biol (2006) 61(3):525–35.10.1007/s11103-006-0027-616830184
99. Lai JS, Messing J. Increasing maize seed methionine by mRNA stability. Plant J (2002) 30:395–402.10.1046/j.1365-313X.2001.01285.x12028570
100. Ramesh SA, Choimes S, Schachtman DP. Over-expression of an Arabidopsis zinc transporter in Hordeum vulgare increases short-term zinc uptake after zinc deprivation and seed zinc content. Plant Mol Biol (2004) 54(3):373–85.10.1023/B:PLAN.0000036370.70912.3415284493
101. Holme IB, Dionisio G, Brinch-Pedersen H, Wendt T, Madsen CK, Vincze E, et al. Cisgenic barley with improved phytase activity. Plant Biotechnol J (2012) 10(2):237–47.10.1111/j.1467-7652.2011.00660.x21955685
102. Ohnoutkova L, Zitka O, Mrizova K, Vaskova J, Galuszka P, Cernei N, et al. Electrophoretic and chromatographic evaluation of transgenic barley expressing a bacterial dihydrodipicolinate synthase. Electrophoresis (2012) 33(15):2365–73.10.1002/elps.20120003322887157
103. Dikeman CL, Fahey GC. Viscosity as related to dietary fiber: a review. Crit Rev Food Sci Nutr (2006) 46:649–63.10.1080/1040839050051186217092830
104. Burton RA, Collins HM, Kibble NA, Smith JA, Shirley NJ, Jobling SA, et al. Over-expression of specific HvCslF cellulose synthase-like genes in transgenic barley increases the levels of cell wall (1,3;1,4)-β-d-glucans and alters their fine structure. Plant Biotechnol J (2011) 9(2):117–35.10.1111/j.1467-7652.2010.00532.x20497371
105. Carciofi M, Blennow A, Jensen SL, Shaik SS, Henriksen A, Buleon A, et al. Concerted suppression of all starch branching enzyme genes in barley produces amylose-only starch granules. BMC Plant Biol (2012) 12(1):223.10.1186/1471-2229-12-22323171412
106. Mihalik D, Gubisova M, Klempova T, Certik M, Ondreickova K, Hudcovicova M, et al. Transgenic barley producing essential polyunsaturated fatty acids. Biol Plant (2014) 58(2):348–54.10.1007/s10535-014-0406-9
107. Kamenarova K, Gecheff K, Stoyanova M, Muhovski Y, Anzai H, Atanassov A, et al. Production of recombinant human lactoferin in transgenic barley. Biotechnol Biotech Eq (2007) 21(1):18–27.10.1080/13102818.2007.10817407
108. Lipkie TE, De Moura FF, Zhao Z-Y, Albertsen MC, Che P, Glassman K, et al. Bioaccessibility of carotenoids from transgenic provitamin A biofortified Sorghum. J Agric Food Chem (2013) 61(24):5764–71.10.1021/jf305361s23692305
109. Zhao ZY, Glassman K, Sewalt V, Wang N, Miller M, Chang S, et al. Nutritionally Improved Transgenic Sorghum. InPlant Biotechnology 2002 and Beyond. Netherlands: Springer (2003). p. 413–6.
110. Elkonin LA, ItalianskayaI JV, Domanina VN, Selivanov NY, Rakitin AL, Ravi NV. Transgenic Sorghum with improved digestibility of storage proteins obtained by Agrobacterium-mediated transformation. Russ J Plant Physiol (2016) 63:678–89.10.1134/S1021443716050046
111. Grootboom AW, Mkhonza NL, Mbambo Z, O’Kennedy MM, Da Silva LS, Taylor J, et al. Co-suppression of synthesis of major α-kafirin sub-class together with γ-kafirin-1 and γ-kafirin-2 required for substantially improved protein digestibility in transgenic Sorghum. Plant Cell Rep (2014) 33(3):521–37.10.1007/s00299-013-1556-524442398
112. Schmidt MA, Parrott WA, Hildebrand DF, Berg RH, Cooksey A, Pendarvis K, et al. Transgenic soya bean seeds accumulating β-carotene exhibit the collateral enhancements of oleate and protein content traits. Plant Biotechnol J (2015) 13(4):590–600.10.1111/pbi.12286
113. Pierce EC, LaFayette PR, Ortega MA, Joyce BL, Kopsell DA, Parrott WA. Ketocarotenoid production in soybean seeds through metabolic engineering. PLoS One (2015) 10(9):e0138196.10.1371/journal.pone.013819626376481
114. Kim MJ, Kim JK, Kim HJ, Pak JH, Lee JH, Kim DH, et al. Genetic modification of the soybean to enhance the β-carotene content through seed-specific expression. PLoS One (2012) 7(10):e48287.10.1371/journal.pone.004828723118971
115. Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, Thorne GM, et al. Engineering vitamin E content: from Arabidopsis mutant to soy oil. Plant Cell (2003) 15:3007–19.10.1105/tpc.01587514630966
116. Kim WS, Chronis D, Juergens M, Schroeder AC, Hyun SW, Jez J, et al. Transgenic soybean plants overexpressing O-acetylserine sulfhydrylase accumulate enhanced levels of cysteine and Bowman-Birk protease inhibitor in seeds. Planta (2012) 235(1):13–23.10.1007/s00425-011-1487-821805150
117. Dinkins RD, Reddy MSS, Meurer CA, Yan B, Trick H, Thibaud-Nissen F, et al. Increased sulfur amino acids in soybean plants overexpressing the maize 15 kDa zein protein. In Vitro Cell Dev Biol Plant (2001) 37:742–7.10.1007/s11627-001-0123-x
118. Song S, Hou W, Godo I, Wu C, Yu Y, Matityahu I, et al. Soybean seeds expressing feedback-insensitive cystathionine γ-synthase exhibit a higher content of methionine. J Exp Bot (2013) 64(7):1917–26.10.1093/jxb/ert05323530130
119. Hanafy MS, Rahman SM, Nakamoto Y, Fujiwara T, Naito S, Wakasa K, et al. Differential response of methionine metabolism in two grain legumes, soybean and azuki bean, expressing a mutated form of Arabidopsis cystathionine γ-synthase. J Plant Physiol (2013) 170(3):338–45.10.1016/j.jplph.2012.10.01823286999
120. Flores T, Karpova O, Su X, Zeng P, Bilyeu K, Sleper DA, et al. Silencing of GmFAD3 gene by siRNA leads to low alpha-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]. Transgenic Res (2008) 17(5):839–50.10.1007/s11248-008-9167-618256901
121. Sato S, Xing A, Ye X, Schweiger B, Kinney A, Graef G, et al. Production of γ-linolenic acid and stearidonic acid in seeds of marker-free transgenic soybean. Crop Sci (2004) 44(2):646–52.10.2135/cropsci2004.0646
122. 6 desaturase and the Arabidopsis delta 15 desaturase results in high accumulation of stearidonic acid in the seeds of transgenic soybean. Planta (2006) 224(5):1050–7.10.1007/s00425-006-0291-3
123. Zhang L, Yang XD, Zhang YY, Yang J, Qi GX, Guo DQ, et al. Changes in oleic acid content of transgenic soybeans by antisense RNA mediated posttranscriptional gene silencing. Int J Genomics (2014) 2014:8.10.1155/2014/92195025197629
124. Yu O, Shi J, Hession AO, Maxwell CA, McGonigle B, Odell JT. Metabolic engineering to increase isoflavone biosynthesis in soybean seed. Phytochemistry (2003) 63:753–63.10.1016/S0031-9422(03)00345-512877915
125. Aragao FJL, Barros LMG, De Sousa MV, Grossi de Sa MF, Almeida ERP, Gander ES, et al. Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae). Genet Mol Biol (1999) 22(3):445–9.10.1590/S1415-47571999000300026
126. Molvig L, Tabe LM, Eggum BO, Moore AE, Craig S, Spencer D, et al. Enhanced methionine levels and increased nutritive value of seeds of transgenic lupins (Lupinus angustifolius L.) expressing a sunflower seed albumin gene. Proc Natl Acad Sci U S A (1997) 94(16):8393–8.10.1073/pnas.94.16.83939237987
127. Ducreux LJM, Morris WL, Hedley PE, Shepherd T, Davies HV, Millam S, et al. Metabolic engineering of high carotenoid potato tubers containing enhanced levels of β-carotene and lutein. J Exp Bot (2004) 56:81–9.10.1093/jxb/eri016
128. Diretto G, Tavazza R, Welsch R, Pizzichini D, Mourgues F, Papacchioli V, et al. Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase. BMC Plant Biol (2006) 6:13.10.1186/1471-2229-6-1316800876
129. Van Eck J, Conlin B, Garvin DF, Mason H, Navarre DA, Brown CR. Enhancing beta-carotene content in potato by rnai-mediated silencing of the beta-carotene hydroxylase gene. Am J Potato Res (2007) 84:331–42.10.1007/BF02986245
130. Song XY, Zhu WJ, Tang RM, Cai JH, Chen M, Yang Q. Over-expression of StLCYb increases β-carotene accumulation in potato tubers. Plant Biotechnol Rep (2016) 10(2):95–104.10.1007/s11816-016-0390-y
131. Lopez AB, Van Eck J, Conlin BJ, Paolillo DJ, O’Neill J, Li L. Effect of the cauliflower or transgene on carotenoid accumulation and chromoplast formation in transgenic potato tubers. J Exp Bot (2008) 59(2):213–23.10.1093/jxb/erm29918256051
132. Romer S, Lubeck J, Kauder F, Steiger S, Adomat C, Sandmann G. Genetic engineering of a zeaxanthin-rich potato by antisense inactivation and co-suppression of carotenoid epoxidation. Metab Eng (2002) 4(4):263–72.10.1006/mben.2002.023412646321
133. Upadhyaya CP, Young KE, Akula N, Soon Kim H, Heung JJ, Oh O, et al. Over-expression of strawberry d-galacturonic acid reductase in potato leads to accumulation of vitamin C with enhanced abiotic stress tolerance. Plant Sci (2009) 177(6):659–67.10.1016/j.plantsci.2009.08.004
134. Dancs G, Kondrak M, Banfalvi Z. The effects of enhanced methionine synthesis on amino acid and anthocyanin content of potato tubers. BMC Plant Biol (2008) 8:65.10.1186/1471-2229-8-6518549488
135. Huang T, Joshi V, Jander G. The catabolic enzyme methionine gamma-lyase limits methionine accumulation in potato tubers. Plant Biotechnol J (2014) 12(7):883–93.10.1111/pbi.1219124738868
136. Zeh M, Casazza AP, Kreft O, Roessner U, Bieberich K, Willmitzer L, et al. Antisense inhibition of threonine synthase leads to high methionine content in transgenic potato plants. Plant Physiol (2001) 127:792–802.10.1104/pp.01043811706163
137. Goo YM, Kim TW, Lee MK, Lee SW. Accumulation of PrLeg, a Perilla legumin protein in potato tuber results in enhanced level of sulphur-containing amino acids. C R Biol (2013) 336(9):433–9.10.1016/j.crvi.2013.09.00224161240
138. Di R, Kim J, Martin MN, Leustek T, Jhoo J, Ho CT, et al. Enhancement of the primary flavor compound methional in potato by increasing the level of soluble methionine. J Agric Food Chem (2003) 51(19):5695–702.10.1021/jf030148c12952421
139. Chakraborty S, Chakraborty N, Agrawal L, Ghosh S, Narula K, Shekhar S, et al. Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber. Proc Natl Acad Sci U S A (2010) 107(41):17533–8.10.1073/pnas.100626510720855595
140. Oakes JV, Shewmaker CK, Stalker DM. Production of cyclodextrins, a novel carbohydrate, in the tubers of transgenic potato plants. Biotechnology (N Y) (1991) 9(10):982–6.10.1038/nbt1091-9821370622
141. Lukaszewicz M, Matysiak-Kata I, Skala J, Fecka I, Cisowski W, Szopa J. Antioxidant capacity manipulation in transgenic potato tuber by changes in phenolic compounds content. J Agric Food Chem (2004) 52:1526–33.10.1021/jf034482k15030206
142. Hellwege EM, Gritscher D, Willmitzer L, Heyer AG. Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom discs. Plant J (1997) 12:1057–65.10.1046/j.1365-313X.1997.12051057.x
143. Hellwege EM, Czapla S, Jahnke A, Willmitzer L, Heyer AG. Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots. Proc Natl Acad Sci U S A (2000) 97:8699–704.10.1073/pnas.15004379710890908
144. Kim SH, Kim YH, Ahn YO, Ahn MJ, Jeong JC, Lee HS, et al. Downregulation of the lycopene ε-cyclase gene increases carotenoid synthesis via the β-branch-specific pathway and enhances salt-stress tolerance in sweetpotato transgenic calli. Physiol Plant (2013) 147(4):432–42.10.1111/j.1399-3054.2012.01688.x
145. Park SC, Kim YH, Kim SH, Jeong YJ, Kim CY, Lee JS, et al. Overexpression of the IbMYB1 gene in an orange-fleshed sweet potato cultivar produces a dual-pigmented transgenic sweet potato with improved antioxidant activity. Physiol Plant (2015) 153(4):525–37.10.1111/ppl.1228125220246
146. Telengech PK, Maling’a JN, Nyende AB, Gichuki ST, Wanjala BW. Gene expression of beta carotene genes in transgenic biofortified cassava. 3 Biotech (2015) 5(4):465–72.10.1007/s13205-014-0243-828324550
147. Welsch R, Arango J, Bar C, Salazar B, Al-Babili S, Beltran J, et al. Provitamin A accumulation in cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene. Plant Cell (2010) 22:3348–56.10.1105/tpc.110.07756020889914
148. Park S, Kim C-K, Pike LM, Smith RH, Hirschi KD. Increased calcium in carrots by expression of an Arabidopsis H+/Ca2+ transporter. Mol Breed (2004) 14:275–82.10.1023/B:MOLB.0000047773.20175.ae
149. Morris J, Hawthorne KM, Hotze T, Abrams SA, Hirschi KD. Nutritional impact of elevated calcium transporter activity in carrots. Proc Natl Acad Sci U S A (2008) 105:1431–5.10.1073/pnas.0709005105
150. Goto F, Yoshihara T, Saiki H. Iron accumulation and enhanced growth in transgenic lettuce plants expressing the iron-binding protein ferritin. Theor Appl Genet (2000) 100:658–64.10.1007/s001220051336
151. Lu S, Van Eck J, Zhou X, Lopez AB, O’Halloran DM, Cosman KM, et al. The cauliflower or gene encodes a DnaJ cysteine-rich domain-containing protein that mediates high levels of β-carotene accumulation. Plant Cell (2006) 18:3594–605.10.1105/tpc.106.046417
152. Lorenc-Kukula K, Wrobel-Kwiatkowska M, Starzycki M, Szopa J. Engineering flax with increased flavonoid content and thus Fusarium resistance. Physiol Mol Plant Pathol (2007) 70:38–48.10.1016/j.pmpp.2007.05.005
153. Galili G, Galili S, Lewinsohn E, Tadmor Y. Genetic, molecular and genomic approaches to improve the value of plant foods and feeds. Crit Rev Plant Sci (2002) 21:167–204.10.1080/0735-260291044232
154. Abbadi A, Domergue F, Bauer J, Napier JA, Welti R, Zähringer U, et al. Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell (2004) 16:2734–48.10.1105/tpc.104.02607015377762
155. Fujisawa M, Watanabe M, Choi SK, Teramoto M, Ohyama K, Misawa N. Enrichment of carotenoids in flaxseed (Linumu sitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtB. J Biosci Bioeng (2008) 105(6):636–41.10.1263/jbb.105.63618640603
156. Ravanello MP, Ke D, Alvarez J, Huang B, Shewmaker CK. Coordinate expression of multiple bacterial carotenoid genes in canola leading to altered carotenoid production. Metab Eng (2003) 5(4):255–63.10.1016/j.ymben.2003.08.00114642353
157. Fujisawa M, Takita E, Harada H, Sakurai N, Suzuki H, Ohyama K, et al. Pathway engineering of Brassica napus seeds using multiple key enzyme genes involved in ketocarotenoid formation. J Exp Bot (2009) 60(4):1319–32.10.1093/jxb/erp00619204032
158. Yu B, Lydiate DJ, Young LW, Schafer UA, Hannoufa A. Enhancing the carotenoid content of Brassica napus seeds by downregulating Lycopene epsilon cyclase. Transgenic Res (2008) 17(4):573–85.10.1007/s11248-007-9131-x17851775
159. Wei S, Li X, Gruber MY, Li R, Zhou R, Zebarjadi A, et al. RNAi-mediated suppression of DET1 alters the levels of carotenoids and sinapate esters in seeds of Brassica napus. J Agric Food Chem (2009) 57(12):5326–33.10.1021/jf803983w19459679
160. Falco SC, Guida T, Locke M, Mauvais J, Sanders C, Ward RT, et al. Transgenic canola and soybean seeds with increased lysine. Nat Biotechnol (1995) 13:577–82.10.1038/nbt0695-5779634796
161. Dehesh K, Jones A, Knutzon DS, Voelker TA. Production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by overexpression of Ch FatB2, a thioesterase cDNA from Cuphea hookeriana. Plant J (1996) 9:167–72.10.1046/j.1365-313X.1996.09020167.x8820604
162. Liu JW, DeMichele S, Bergana M, Bobik E, Hastilow C, Chuang LT, et al. Characterization of oil exhibiting high γ-linolenic acid from a genetically transformed canola strain. J Am Oil Chem Soc (2001) 78(5):489–93.10.1007/s11746-001-0291-2
163. Flider FJ. GLA: uses and new sources. Inform (2005) 16(5):279–82.
164. Hong H, Datla N, Reed DW, Covello PS, MacKenzie SL, Qiu X. High-level production of γ-linolenic acid in Brassica juncea using a Δ6 desaturase from pythium irregular. Plant Physiol (2002) 129(1):354–62.10.1104/pp.001495
165. Enfissi E, Fraser PD, Lois LM, Boronat A, Schuch W, Bramley PM. Metabolic engineering of the mevalonate and nonmevalonate isopentenyl diphosphate-forming pathways for the production of health-promoting isoprenoids in tomato. Plant Biotechnol J (2005) 3:17–27.10.1111/j.1467-7652.2004.00091.x
166. Fraser PD, Enfissi EM, Halket JM, Truesdale MR, Yu D, Gerrish C, et al. Manipulation of phytoene levels in tomato fruit: effects on isoprenoids, plastids, and intermediary metabolism. Plant Cell (2007) 19(10):3194–211.10.1105/tpc.106.04981717933904
167. Rosati C, Aquilani R, Dharmapuri S, Pallara P, Marusic C, Tavazza R, et al. Metabolic engineering of β-carotene and lycopene content in tomato fruit. Plant J (2000) 24:413–9.10.1046/j.1365-313x.2000.00880.x
168. Apel W, Bock R. Enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin A conversion. Plant Physiol (2009) 151(1):59–66.10.1104/pp.109.14053319587100
169. Wurbs D, Rup S, Bock R. Contained metabolic engineering in tomatoes by expression of carotenoid biosynthesis genes from the plastid genome. Plant J (2007) 49(2):276–88.10.1111/j.1365-313X.2006.02960.x17241450
170. Huang JC, Zhong YJ, Liu J, Sandmann G, Chen F. Metabolic engineering of tomato for high-yield production of astaxanthin. Metab Eng (2013) 17:59–67.10.1016/j.ymben.2013.02.00523511430
171. Dharmapuri S, Rosati C, Pallara P, Aquilani R, Bouvier F, Camara B, et al. Metabolic engineering of xanthophyll content in tomato fruits. FEBS Lett (2002) 519(1–3):30–4.10.1016/S0014-5793(02)02699-612023013
172. Davuluri GR, Van Tuinen A, Fraser PD, Manfredonia A, Newman R, Burgess D, et al. Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes. Nat Biotechnol (2005) 23:890–5.10.1038/nbt110815951803
173. Zhang C, Liu J, Zhang Y, Cai X, Gong P, Zhang J, et al. Overexpression of SlGMEs leads to ascorbate accumulation with enhanced oxidative stress, cold, and salt tolerance in tomato. Plant Cell Rep (2011) 30:389–98.10.1007/s00299-010-0939-020981454
174. Haroldsen VM, Chi-Ham CL, Kulkarni S, Lorence A, Bennet AB. Constitutively expressed DHAR and MDHAR influence fruit, but not foliar ascorbate levels in tomato. Plant Physiol Biochem (2011) 49:1244–9.10.1016/j.plaphy.2011.08.00321875809
175. Cronje C, George GM, Fernie AR, Bekker J, Kossmann J, Bauer R. Manipulation of L-ascorbic acid biosynthesis pathways in Solanum lycopersicum elevated GDP-mannose pyrophosphorylase activity enhances L-ascorbate levels in red fruit. Planta (2012) 235:553–64.10.1007/s00425-011-1525-621979413
176. De la Graza RD, Quinlivan LP, Klaus MJS, Basset GJC, Gregory JF, III Hanson AD. Folate biofortification in tomatoes by engineering the pteridine branch of folate synthesis. Proc Natl Acad Sci U S A (2004) 101(38):13720–5.10.1073/pnas.040420810115365185
177. De la Graza RD, Gregory JF, III Hanson AD. Folate biofortification of tomato fruit. Proc Natl Acad Sci U S A (2007) 104(10):4218–22.10.1073/pnas.070040910417360503
178. Muir SR, Collins GJ, Robinson S, Hughes S, Bovy A, Ric De Vo CH, et al. Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols. Nature (2001) 19:470–4.10.1038/8815011329019
179. Zuluaga DL, Gonzali S, Loreti E, Pucciariello C, Degl’Innocenti E, Guidi L, et al. Arabidopsis thaliana MYB75/PAP1 transcription factor induces anthocyanin production in transgenic tomato plants. Funct Plant Biol (2008) 35(7):606–18.10.1071/FP08021
180. Niggeweg R, Michael AJ, Martin C. Engineering plants with increased levels of the antioxidant chlorogenic acid. Nat Biotechnol (2004) 22:746–54.10.1038/nbt96615107863
181. Giovinazzo G, D’Amico L, Paradiso A, Bollini R, Sparvoli F, DeGara L. Antioxidant metabolite profiles in tomato fruit constitutively expressing the grapevine stilbene synthase gene. Plant Biotechnol J (2005) 3(1):57–69.10.1111/j.1467-7652.2004.00099.x17168899
182. Luo J, Butelli E, Hill L, Parr A, Niggeweg R, Bailey P, et al. AtMYB12 regulates caffeoyl quinic acid and flavonol synthesis in tomato: expression in fruit results in very high levels of both types of polyphenol. Plant J (2008) 56(2):316–26.10.1111/j.1365-313X.2008.03597.x18643978
183. Shih CH, Chen Y, Wang M, Chu IK, Lo C. Accumulation of isoflavone genistin in transgenic tomato plants overexpressing a soybean isoflavone synthase gene. J Agric Food Chem (2008) 56(14):5655–61.10.1021/jf800423u18540614
184. Szankowski I, Briviba K, Fleschhut J, Schönherr J, Jacobsen HJ, Kiesecker H. Transformation of apple (Malus domestica Borkh.) with the stilbene synthase gene from grapevine (Vitis vinifera L.) and a PGIP gene from kiwi (Actinidia deliciosa). Plant Cell Rep (2003) 22:141–9.10.1007/s00299-003-0668-814504909
185. Waltz E. Vitamin A super banana in human trials. Nat Biotechnol (2014) 32:857.10.1038/nbt0914-857
186. Deavours BE, Dixon RA. Metabolic engineering of isoflavonoid biosynthesis in alfalfa. Plant Physiol (2005) 138(4):2245–59.10.1104/pp.105.06253916006598
187. Avaram T, Badani H, Galili S, Aamir R. Enhanced levels of methionine and cysteine in transgenic alfalfa (Medicago sativa L.) plants over-expressing the Arabidopsis cystathionine γ-synthase gene. Plant Biotechnol J (2005) 3:71–9.10.1111/j.1467-7652.2004.00102.x
188. Reddy MS, Chen F, Shadle G, Jackson L, Aljoe H, Dixon RA. Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.). Proc Natl Acad Sci U S A (2005) 102:16573–8.10.1073/pnas.050574910216263933
189. Austin-Phillips S, Koegel RG, Straub RJ, Cook M. Animal Feed Compositions Containing Phytase Derived from Transgenic Alfalfa and Methods of Use Thereof. United States patent US 6248938 (2001).
190. Bibbins-Domingo K, Grossman DC, Curry SJ, Davidson KW, Epling JW, Garcia FA, et al. Folic acid supplementation for the prevention of neural tube defects US preventive services task force recommendation statement. JAMA (2017) 317(2):183–9.10.1001/jama.2016.1943828097362
191. Lee S, Jeon US, Lee SJ, Kim YK, Persson DP, Husted S, et al. Iron fortification of rice seeds through activation of the nicotianamine synthase gene. Proc Natl Acad Sci U S A (2009) 106:22014–9.10.1073/pnas.091095010620080803
192. Crawford M, Galli C, Visioli F, Renaud S, Simopoulos AP, Spector AA. Role of plant-derived omega-3 fatty acids in human nutrition. Ann Nutr Metab (2000) 44:263–5.10.1159/00004669411146334
193. Lee JH, Kim IG, Kim HS, Shin KS, Suh SC, Kweon SJ, et al. Development of transgenic rice lines expressing the human lactoferrin gene. J Plant Biotechnol (2010) 37(4):556–61.10.5010/JPB.2010.37.4.556
194. Zhu C, Naqvi S, Breitenbach J, Sandmann G, Christou P, Capell T. Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize. Proc Natl Acad Sci U S A (2008) 105(47):18232–7.10.1073/pnas.080973710519011084
195. Dutton HJ, Lancaster CJ, Evans CD, Cowan JC. The flavor problem of soybean oil. VIII. Linolenic acid. J Am Oil Chem Soc (1951) 28:115–8.10.1007/BF02612206
196. Watanabe S, Uesugi S, Kikuchi Y. Isoflavones for prevention of cancer, cardiovascular diseases, gynecological problems and possible immune potentiation. Biomed Pharmacother (2002) 56:302–12.10.1016/S0753-3322(02)00182-812224602
197. Teow CC, Truong VD, McFeeters RF, Thompson RL, Pecota KV, Yencho GC. Antioxidant activities, phenolic and b-carotene contents of sweet potato genotypes with varying flesh colours. Food Chem (2007) 103:829–38.10.1016/j.foodchem.2006.09.033
198. Miller GD, Jarvis JK, McBean LD. The importance of meeting calcium needs with food. J Am Coll Nutr (2001) 20:168–85.10.1080/07315724.2001.10719029
199. Newton IS. Long-chain polyunsaturated fatty acids—the new frontier in nutrition. Lipid Technol (1998) 10:77–81.
200. Harborne JB. Recent advances in the ecological chemistry of plant terpenoids. Ecol Chem Biochem Plant Terpenoids (1991) 6:399–426.
201. Maligeppagol M, Chandra GS, Navale PM, Deepa H, Rajeev PR, Asokan R, et al. Anthocyanin enrichment of tomato (Solanum lycopersicum L.) fruit by metabolic engineering. Curr Sci (2013) 105(1):72–80.
202. Cakmak I, Kutman UB. Agronomic biofortification of cereals with zinc: a review. Eur J Soil Sci (2017) 69:172–80.10.1111/ejss.12437
203. Daniels LA. Selenium metabolism and bioavailability. Biol Trace Elem Res (1996) 54(3):185–99.10.1007/BF027844308909692
204. Erisman JW, Sutton MA, Galloway JN, Klimont Z, Winiwarter W. How a century of ammonia synthesis changed the world. Nat Geo Sci (2008) 1:636–9.10.1038/ngeo325
205. Graham RD, Welch RM, Saunders DA, Ortiz-Monasterio I, Bouis HE, Bonierbale M, et al. Nutritious subsistence food systems. Adv Agron (2007) 92:1–74.10.2134/agronj2005.0222
206. Cakmak I. Enrichment of cereal grains with zinc: agronomic or genetic biofortification. Plant Soil (2008) 302:1–17.10.1007/s11104-008-9584-6
207. Aro A, Alfthan G, Varo P. Effects of supplementation of fertilizers on human selenium status in Finland. Analyst (1995) 120:841–3.10.1039/an9952000841
208. Cakmak I, Kalaycı M, Ekiz H, Braun HJ, Kılınç Y, Yılmaz A. Zinc deficiency as a practical problem in plant and human nutrition in Turkey: a NATO-science for stability project. Field Crops Res (1999) 60:175–88.10.1016/S0378-4290(98)00139-7
209. Jiang XM, Cao XY, Jiang JY, Ma T, James DW, Rakeman MA, et al. Dynamics of environmental supplementation of iodine: four years’ experience in iodination of irrigation water in Hotien, Xinjiang, China. Arch Environ Health (1997) 52(6):399–408.10.1080/00039899709602218
210. Rengel Z, Batten GD, Crowley DE. Agronomic approaches for improving the micronutrient density in edible portions of field crops. Field Crops Res (1999) 60:27–40.10.1016/S0378-4290(98)00131-2
211. Smith SE, Read DJ. Mycorrhizal Symbiosis. 3rd ed. London, UK: Elsevier (2007).
212. Hardarson G, Broughton WJ. Maximising the use of biological nitrogen fixation in agriculture. Ann Bot (2004) 93(4):477.10.1093/aob/mch065
213. Cavagnaro TR. The role of arbuscular mycorrhizas in improving plant zinc nutrition under low soil zinc concentrations: a review. Plant Soil (2008) 304:315–25.10.1007/s11104-008-9559-7
214. He W, Shohag MJ, Wei Y, Feng Y, Yang X. Iron concentration, bioavailability, and nutritional quality of polished rice affected by different forms of foliar iron fertilizer. Food Chem (2013) 141(4):4122–6.10.1016/j.foodchem.2013.07.00523993594
215. Yuan L, Wu L, Yang C, Quin LV. Effects of iron and zinc foliar applications on rice plants and their grain accumulation and grain nutritional quality. J Sci Food Agric (2013) 93(2):254–61.10.1002/jsfa.574922740351
216. Fang Y, Wang L, Xin Z, Zhao L, An X, Hu Q. Effect of foliar application of zinc, selenium, and iron fertilizers on nutrients concentration and yield of rice grain in China. J Agric Food Chem (2008) 6(56):2079–84.10.1021/jf800150z18311920
217. Wei Y, Shohag MJ, Yang X, Yibin Z. Effects of foliar iron application on iron concentration in polished rice grain and its bioavailability. J Agric Food Chem (2012) 60(45):11433–9.10.1021/jf3036462
218. Wei Y, Shohag MJ, Yang X. Biofortification and bioavailability of rice grain zinc as affected by different forms of foliar zinc fertilization. PLoS One (2012) 7(9):e45428.10.1371/journal.pone.0045428
219. Boonchuay P, Cakmak I, Rerkasem B, Prom-U-Thai C. Effect of different foliar zinc application at different growth stages on seed zinc concentration and its impact on seedling vigor in rice. Soil Sci Plant Nutr (2013) 59(2):180–8.10.1080/00380768.2013.763382
220. Jiang W, Struik PC, Keulen HV, Zhao M, Jin LN, Stomph TJ. Does increased zinc uptake enhance grain zinc mass concentration in rice? Ann Appl Biol (2008) 153(1):135–47.10.1111/j.1744-7348.2008.00243.x
221. Mabesa RL, Impa SM, Grewal D, Beebout SEJ. Contrasting grain-Zn response of biofortification rice (Oryza sativa L.) breeding lines to foliar Zn application. Field Crops Res (2013) 149:223–33.10.1016/j.fcr.2013.05.012
222. Shivay YS, Kumar D, Prasad R, Ahlawat IPS. Relative yield and zinc uptake by rice from zinc sulphate and zinc oxide coatings onto urea. Nutr Cycl Agroecosys (2008) 80(2):181–8.10.1007/s10705-007-9131-5
223. Ram H, Rashid A, Zhang W, Duarte AP, Phattarakul N, Simunji S, et al. Biofortification of wheat, rice and common bean by applying foliar zinc fertilizer along with pesticides in seven countries. Plant Soil (2016) 1(403):389–401.10.1007/s11104-016-2815-3
224. Guo JX, Feng XM, Hu XY, Tian GL. Effects of soil zinc availability, nitrogen fertilizer rate and zinc fertilizer application method on zinc biofortification of rice. J Agric Sci (2016) 154(4):584–97.10.1017/S0021859615000441
225. Chen L, Yang F, Xu J, Hu Y, Hu Q, Zhang Y, et al. Determination of selenium concentration of rice in china and effect of fertilization of selenite and selenate on selenium content of rice. J Agric Food Chem (2002) 50(18):5128–30.10.1021/jf020137412188618
226. Ros GH, VanRotterdm AMD, Bussink DW, Bindraban PS. Selenium fertilization strategies for bio-fortification of food: an agro-ecosystem approach. Plant Soil (2016) 404:99–112.10.1007/s11104-016-2830-4
227. Premarathna L, McLaughlin MJ, Kirby JK, Hettiarachchi GM, Stacey S, Chittleborough DJ. Selenate-enriched urea granules are a highly effective fertilizer for selenium biofortification of paddy rice grain. J Agric Food Chem (2012) 60(23):6037–44.10.1021/jf300578822630040
228. Xu J, Hu Q. Effect of foliar application of selenium on the antioxidant activity of aqueous and ethanolic extracts of selenium-enriched rice. J Agric Food Chem (2004) 52(6):1759–63.10.1021/jf034983615030242
229. Giacosa A, Faliva MA, Perna S, Minoia C, Ronchi A, Rondanelli M. Selenium fortification of an Italian rice cultivar via foliar fertilization with sodium selenate and its effects on human serum selenium levels and on erythrocyte glutathione peroxidase activity. Nutrients (2014) 6(3):1251–61.10.3390/nu603125124667132
230. Liu K, Gu Z. Selenium accumulation in different brown rice cultivars and its distribution in fractions. J Agric Food Chem (2009) 57(2):695–700.10.1021/jf802948k19154168
231. Aciksoz SB, Yazici A, Ozturk L, Cakmak I. Biofortification of wheat with iron through soil and foliar application of nitrogen and iron fertilizers. Plant Soil (2011) 349(1):215–25.10.1007/s11104-011-0863-2
232. Cakmak I. Biofortification of cereals with zinc and iron through fertilization strategy. In 19th World Congress of Soil Science, Soil Solutions for a Changing World (2010) Vol. 5. p. 1–6.
233. Yang XW, Tian XH, Lu XC, Cao YX, Chen ZH. Impacts of phosphorus and zinc levels on phosphorus and zinc nutrition and phytic acid concentration in wheat (Triticum aestivum L.). J Sci Food Agric (2011) 91(13):2322–8.10.1002/jsfa.445921547926
234. Nooria M, Adibiana M, Sobhkhizia A, Eyidozehib K. Effect of phosphorus fertilizer and mycorrhiza on protein percent, dry weight, weight of 1000 grain in wheat. Int J Plant Anim Environ Sci (2014) 4(2):561–4.
235. Ramzani PMA, Khalid M, Naveed M, Ahmad R, Shahid M. Iron biofortification of wheat grains through integrated use of organic and chemical fertilizers in pH affected calcareous soil. Plant Physiol Biochem (2016) 104:284–93.10.1016/j.plaphy.2016.04.05327179316
236. Ramesh A, Sharma SK, Sharma MP, Yadav N, Joshi OP. Inoculation of zinc solubilizing Bacillus aryabhattai strains for improved growth, mobilization and biofortification of zinc in soybean and wheat cultivated in vertisols of central India. Appl Soil Ecol (2014) 73:87–96.10.1016/j.apsoil.2013.08.009
237. Alvarez JM, Rico MI. Effects of zinc complexes on the distribution of zinc in calcareous soil and zinc uptake by maize. J Agric Food Chem (2003) 51(19):5760–7.10.1021/jf030092m12952430
238. Lopez-Valdivia LM, Fernandez MD, Obrador A, Alvarez JM. Zinc transformations in acidic soil and zinc efficiency on maize by adding six organic zinc complexes. J Agric Food Chem (2002) 50(6):1455–60.
239. Fahad S, Hussain S, Saud S, Hassan S, Shan D, Chen Y, et al. Grain cadmium and zinc concentrations in maize influenced by genotypic variations and zinc fertilization. Clean Soil Air Water (2015) 43(10):1433–40.10.1002/clen.201400376
240. Wang J, Mao H, Zhao H, Huang D, Wang Z. Different increases in maize and wheat grain zinc concentrations caused by soil and foliar applications of zinc in Loess plateau, China. Field Crops Res (2012) 135:89–96.10.1016/j.fcr.2012.07.010
241. Zhang YQ, Pang LL, Yan P, Liu DY, Zhang W, Yost R, et al. Zinc fertilizer placement affects zinc content in maize plant. Plant Soil (2013) 372:81–92.10.1007/s11104-013-1904-9
242. Prasanna R, Bidyarani N, Babu S, Hossain F, Shivay YS, Nain L. Cyanobacterial inoculation elicits plant defence response and enhanced Zn mobilization in maize hybrids. Cogent Food Agric (2015) 1(1):998507.10.1080/23311932.2014.998507
243. Maleki FS, Chaichi MR, Mazaheri D, Tavakkol AR, Savaghebi G. Barley grain mineral analysis as affected by different fertilizing systems and by drought stress. J Agric Sci Tec (2011) 13:315–26.
244. Dhawi F, Datta R, Ramakrishna W. Mycorrhiza and PGPB modulate maize biomass, nutrient uptake and metabolic pathways in maize grown in mining-impacted soil. Plant Physiol Biochem (2015) 97:390–9.10.1016/j.plaphy.2015.10.02826546782
245. Dhawi F, Datta R, Ramakrishna W. Mycorrhiza and heavy metal resistant bacteria enhance growth, nutrient uptake and alter metabolic profile of Sorghum grown in marginal soil. Chemosphere (2016) 157:33–41.10.1016/j.chemosphere.2016.04.11227208643
246. Patidar M, Mali AL. Effect of farmyard manure, fertility levels and bio-fertilizers on growth, yield and quality of Sorghum (Sorghum bicolor). In J Agron (2004) 2(49):117–20.
247. Yang F, Chen L, Hu Q, Pan G. Effect of the application of selenium on selenium content of soybean and its products. Biol Trace Elem Res (2003) 93(1–3):249–56.10.1385/BTER:93:1-3:24912835506
248. Sathya A, Vijayabharati R, Srinivas V, Gopalakrishnan S. Plant growth-promoting action-bacteria on chickpea seed mineral density: an upcoming complementary tool for sustainable biofortification strategy. 3 Biotech (2013) 6(2):138.10.1007/s13205-016-0458-y
249. Pellegrino E, Bedini S. Enhancing ecosystem services in sustainable agriculture: biofertilization and biofortification of chickpea (Cicer arietinum L.) by arbuscular mycorrhizal fungi. Soil Biol Biochem (2014) 68:429–39.10.1016/j.soilbio.2013.09.030
250. Shivay YS, Prasad R, Pal M. Effects of source and method of zinc application on yield, zinc biofortification of grain, and Zn uptake and use efficiency in chickpea (Cicer arietinum L.). Commun Soil Sci Plant Anal (2015) 46(17):2191–200.10.1080/00103624.2015.1069320
251. Poblaciones MJ, Rodrigo S, Santamaria O, Chen Y, McGrath SP. Selenium accumulation and speciation in biofortified chickpea (Cicer arietinum L.) under Mediterranean conditions. J Sci Food Agric (2014) 94(6):1101–6.10.1002/jsfa.637223983062
252. Poblaciones MJ, Rengel Z. Soil and foliar zinc biofortification in field pea (Pisum sativum L.). Grain accumulation and bioavailability in raw and cooked grains. Food Chem (2016) 212:427–33.10.1016/j.foodchem.2016.05.18927374552
253. Ibrahim EA, Ramadan WA. Effect of zinc foliar spray alone and combined with humic acid or/and chitosan on growth, nutrient elements content and yield of dry bean (Phaseolus vulgaris L.) plants sown at different dates. Sci Hortic (2015) 184:101–15.10.1016/j.scienta.2014.11.010
254. Westermann DT, Teran H, Munoz-Perea CG, Singh SP. Plant and seed nutrient uptake in common bean in seven organic and conventional production systems. Can J Plant Sci (2011) 91:1089–99.10.4141/cjps10114
255. Yasin M, El Mehdawi AF, Jahn CE, Anwar A, Turner MF, Faisal M, et al. Seleniferous soils as a source for production of selenium-enriched foods and potential of bacteria to enhance plant selenium uptake. Plant Soil (2015) 386:385–94.10.1007/s11104-014-2270-y
256. Poggi V, Arcioni A, Filippini P, Pifferi PG. Foliar application of selenite and selenate to potato (Solanum tuberosum): effect of a ligand agent on selenium content of tubers. J Agric Food Chem (2000) 48(10):4749–51.10.1021/jf000368f11052729
257. Cuderman P, Kreft I, Germ M, Kovacevic M, Stibilj V. Selenium species in selenium-enriched and drought-exposed potatoes. J Agric Food Chem (2008) 56(19):9114–20.10.1021/jf801496918795781
258. Laurie SM, Faber M, Van Jaarsveld PJ, Laurie RN, Du Plooy CP, Modisane PC. β-Carotene yield and productivity of orange-fleshed sweet potato (Ipomoea batatas L. Lam.) as influenced by irrigation and fertilizer application treatments. Sci Hortic (2012) 142:180–4.10.1016/j.scienta.2012.05.017
259. Smolen S, Skoczylas L, Ledwozyw-Smolen L, Rakoczy R, Kopec A, Piatkowska E, et al. Biofortification of carrot (Daucus carota L.) with iodine and selenium in a field experiment. Front Plant Sci (2016) 7:730.10.3389/fpls.2016.0073027303423
260. Smolen S, Kowalska L, Sady W. Assessment of biofortification with iodine and selenium of lettuce cultivated in the NFT hydroponic system. Sci Hortic (2014) 166:9–16.10.1016/j.scienta.2013.11.011
261. Carvalho KM, Gallardo-Williams MT, Benson RF, Martin DF. Effects of selenium supplementation on four agricultural crops. J Agric Food Chem (2003) 51:704–9.10.1021/jf025855512537445
262. Landini M, Gonzali S, Perata P. Iodine biofortification in tomato. J Plant Nutr Soil Sci (2011) 174(3):480–6.10.1002/jpln.201000395
263. Nosheen A, Bano A, Ullah F. Nutritive value of canola (Brassica napus L.) as affected by plant growth promoting rhizobacteria. Eur J Lipid Sci Tech (2011) 113(11):1342–6.10.1002/ejlt.201000549
264. White PJ, Thompson JA, Wright G, Rasmussen SK. Biofortifying Scottish potatoes with zinc. Plant Sci (2017) 411(1):151–65.10.1007/s11104-016-2903-4
265. Fardart A. New hypotheses for the health-protective mechanisms of whole-grain cereals: what is beyond fibre? Nutr Res Rev (2010) 23(1):65–134.10.1017/S095442241000004120565994
266. Tighe P, Duthie G, Vaughan N, Brittenden J, Simpson WG, Duthie S, et al. Effect of increased consumption of whole-grain foods on blood pressure and other cardiovascular risk markers in healthy middle-aged persons: a randomized controlled trial. Am J Clin Nutr (2010) 92(4):733–40.10.3945/ajcn.2010.29417
267. Lafiandra D, Riccardi G, Shewry PR. Improving cereal grain carbohydrates for diet and health. J Cereal Sci (2014) 59:312–26.10.1016/j.jcs.2014.01.00124966450
268. Bouis HE, Welch RM. Biofortification—a sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop Sci (2010) 50:S20–32.10.2135/cropsci2009.09.0531
269. Gregorio GB, Senadhira D, Htut H, Graham RD. Breeding for trace mineral density in rice. Food Nutr Bull (2000) 21:382–6.10.1177/156482650002100407
270. Monasterio I, Graham RD. Breeding for trace minerals in wheat. Food Nutr Bull (2000) 21(4):392–6.10.1177/156482650002100409
271. Welch RM, House RA, Ortiz-Monasterio I, Cheng Z. Potential for improving bioavailable zinc in wheat grain (Triticum species) through plant breeding. J Agric Food Chem (2005) 53:2176–80.10.1021/jf040238x15769153
272. Cakmak I, Torun A, Millet E, Feldman M, Fahima T, Korol A, et al. Triticum dicoccoides: an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat. Soil Sci Plant Nutr (2004) 50:1047–54.10.1080/00380768.2004.10408573
273. Digesu AM, Platani C, Cattivelli L, Mangini G, Blanco A. Genetic variability in yellow pigment components in cultivated and wild tetraploid wheats. J Cereal Sci (2009) 50:210–8.10.1016/j.jcs.2009.05.002
274. Ficco DB, Mastrangelo AM, Trono D, Borrelli GM, De Vita P, Fares C, et al. The colours of durum wheat: a review. Crop Pasture Sci (2014) 65(1):1–15.10.1071/CP13293
275. Garg M, Chawla M, Chunduri V, Kumar R, Sharma S, Sharma NK, et al. Transfer of grain colors to elite wheat cultivars and their characterization. J Cereal Sci (2016) 71:138–44.10.1016/j.jcs.2016.08.004
276. Havrlentova M, Psenakova I, Zofajova A, Ruckschloss L, Kraic J. Anthocyanins in wheat seed – a mini review. Nova Biotechnol Chim (2014) 13(1):1–12.10.2478/nbec-2014-0001
277. Martinek P, Jirsa O, Vaculova K, Chrpova J, Watanabe N, Buresova V, et al. Use of wheat gene resources with different grain colour in breeding. Tagung Ver Pflanzenzüchter Saatgutkaufleute Osterreichs (2013–2014) 64(1):75–8.
278. Palmer AC, Healy K, Barffour MA, Siamusantu W, Chileshe J, Schulze KJ, et al. Provitamin A carotenoid-biofortified maize consumption increases pupillary responsiveness among Zambian children in a randomized controlled trial. J Nutr (2016) 146(12):2551–8.10.3945/jn.116.23920227798345
279. Muzhingi T, Palacios N, Miranda A, Cabrera ML, Yeum KJ, Tang G. Genetic variation of carotenoids, vitamin E and phenolic compounds in biofortified maize. J Sci Food Agric (2016) 97(3):793–801.10.1002/jsfa.7798
280. Lago C, Cassani E, Zanzi C, Pilu R. Development and study of a maize cultivar rich in anthocyanins: coloured polenta, a new functional food. Plant Breed (2014) 133(2):210–7.10.1111/pbr.12153
281. Goffman FD, Bohme T. Relationship between fatty acid profile and vitamin E content in maize hybrids (Zea mays L.). J Agric Food Chem (2001) 49(10):4990–4.10.1021/jf010156y11600056
282. Reddy BVS, Ramesh S, Longvah T. Prospects of breeding for micronutrients and β-carotene-dense sorghums. Int Sorghum Millets Newsl (2005) 46:10–4.
283. Velu G, Rai KN, Muralidharan V, Kulkarni VN, Longvah T, Raveendran TS. Prospects of breeding biofortified pearl millet with high grain iron and zinc content. Plant Breed (2007) 126:182–5.10.1111/j.1439-0523.2007.01322.x
284. Rai KN, Govindraj M, Rao AS. Genetic enhancement of grain iron and zinc content in pearl millet. Crops Food (2012) 4(3):119–25.10.1111/j.1757-837X.2012.00135.x
285. Blair MW, Astudillo C, Grusak MA, Graham R, Beebe SE. Inheritance of seed iron and zinc concentrations in common bean (Phaseolus vulgaris L.). Mol Breed (2009) 23(2):197–207.10.1007/s11032-008-9225-z
286. Gelin JR, Forster S, Grafton KF, McClean P, Rojas-Cifuentes GA. Analysis of seed-zinc and other nutrients in a recombinant inbred population of navy bean (Phaseolus vulgaris L.). Crop Sci (2006) 47:1361–6.10.2135/cropsci2006.08.0510
287. Beebe S, Gonzalez AV, Rengifo J. Research on trace minerals in the common bean. Food Nutr Bull (2000) 21:387–91.10.1177/156482650002100408
288. Lachman J, Hamouz K. Red and purple coloured potatoes as a significant antioxidant source in human nutrition – a review. Plant Soil Environ (2005) 51:477–82.
289. Andre CM, Ghislain MP, Bertin O, Mouhssin M, Del Rosario H, Hoffmann L, et al. Andean potato cultivars (Solanum tuberosum L.) as a source of antioxidant and mineral micronutrients. J Agric Food Chem (2007) 55(2):366–78.10.1021/jf062740i17227067
290. Burgos G, Amoros W, Morote M, Stangoulis J, Bonierbale M. Fe and Zn concentration of native Andean potato cultivars from a human nutrition perspective. J Food Sci Agric (2007) 87:668–75.10.1002/jsfa.2765
291. Brown CR, Haynes KG, Moore M, Pavek MJ, Hane DC, Love SL, et al. Stability and broad-sense heritability of mineral content in potato: iron. Am J Potato Res (2010) 87(4):390–6.10.1007/s12230-010-9145-4
292. Haynes KG, Yencho GC, Clough ME, Henninger MR, Sterrett SB. Genetic variation for potato tuber micronutrient content and implications for biofortification of potatoes to reduce micronutrient malnutrition. Am J Potato Res (2012) 89:192–8.10.1007/s12230-012-9242-7
293. Kumagai T, Umemura Y, Baba T, Iwanaga M. The inheritance of β-amylase null in storage roots of sweet potato, (Ipomoea batatas L.). Theor Appl Genet (1990) 79(3):369–76.10.1007/BF01186081
294. Maziya-Dixon B, Kling JG, Menkir A, Dixon A. Genetic variation in total carotene, iron, and zinc contents of maize and cassava genotypes. Food Nutr Bull (2000) 21:419–22.10.1177/156482650002100415
295. Chavez AL, Sanchez T, Jaramillo G, Bedoya JM, Echeverry J, Bolanos EA, et al. Variation of quality traits in cassava roots evaluated in landraces and improved clones. Euphytica (2005) 143(1–2):125–33.10.1007/s10681-005-3057-2
296. Mazzucato A, Papa R, Bitocchi E, Mosconi P, Nanni R, Negri V, et al. Genetic diversity, structure and marker-trait associations in a collection of Italian tomato (Solanum lycopersicum L.) landraces. Theor Appl Genet (2008) 116(5):657–69.10.1007/s00122-007-0699-618193185
297. Ortiz-Monasterio JI, Rojas NP, Meng E, Pixley K, Trethowan R, Pena RJ. Enhancing the mineral and vitamin content of wheat and maize through plant breeding. J Cereal Sci (2007) 46(3):293–307.10.1016/j.jcs.2007.06.005
298. Li W, Beta T, Sun S, Corke H. Protein characteristics of Chinese black-grained wheat. Food Chem (2006) 98:463–72.10.1016/j.foodchem.2005.06.020
299. Eticha F, Grausgruber H, Siebenhandl-ehn S, Berghofer E. Some agronomic and chemical traits of blue aleurone and purple pericarp wheat (Triticum L.). J Agric Sci Technol (2011) 1:48–58.
300. Pixley K, Palacios-Rojas N, Babu R, Mutale R, Surles R, Simpungwe E. Biofortification of maize with provitamin A carotenoids. In: Tanumihardjo SA, editor. Carotenoids and Human Health. New York: Springer Science (2013). p. 271–92.
301. CIMMYT. Biofortification to Fight “Hidden Hunger” in Zimbabwe. (2016). Available from: http://www.cimmyt.org/biofortification-to-fight-hidden-hunger-in-zimbabwe/
302. Waters BM, Pedersen JF. Sorghum germplasm profiling to assist breeding and gene identification for biofortification of grain mineral and protein concentrations. The Proceedings of the International Plant Nutrition Colloquium XVI. California (2009).
303. Fernandez MS, Kapran I, Souley S, Abdou M, Maiga IH, Acharya CB, et al. Collection and characterization of yellow endosperm fertilizers on human selenium status in Finland. Analyst (2009) 120:841–3.10.1007/s10722-009-9417-3
304. Kumar AA, Reddy BVS, Ramaiah B. Biofortification for combating micronutrient malnutrition: identification of commercial Sorghum cultivars with high grain iron and zinc concentrations. Indian J Dryland Agric Dev (2013) 28(1):89–94.
305. Rao PP, Birthal PS, Reddy BVS, Rai KN, Ramesh S. Diagnostics of Sorghum and pearl millet grains-based nutrition in India. Int Sorghum Millets Newsl (2006) 47:93–6.
306. Sarker A, Agrawal SK. Combating Micronutrient Malnutrition with Biofortified Lentils. Amman Jordan the International Center for Agriculture Research in the Dry Areas. The International Center for Agriculture Research in the Dry Areas (2015).
307. Thavarajah D, Ruszkowski J, Vandenberg A. High potential for selenium biofortification of lentils (Lens culinaris L.). J Agric Food Chem (2008) 56(22):10747–53.10.1021/jf802307h18954072
308. Petry N, Boy E, Wirth JP, Hurrell RF. Review: the potential of the common bean (Phaseolus vulgaris) as a vehicle for iron biofortification. Nutrients (2015) 7(2):1144–73.10.3390/nu702114425679229
309. Broadley M, Lochlainn S, Hammond J, Bowen H, Cakmak I, Eker S. Shoot zinc (Zn) concentration varies widely within Brassica oleracea L. and is affected by soil Zn and phosphorus (P) levels. J Hortic Sci (2010) 85(5):375–80.10.1080/14620316.2010.11512683
310. Rick CM, Chetelat RT. Utilization of related wild species for tomato improvement. Acta Hortic (1995) 412:21–38.10.17660/ActaHortic.1995.412.1
311. Lauricella M, Emanuele S, Calvaruso G, Giuliano M, D’Anneo A. Multifaceted health benefits of Mangifera indica L. (Mango): the inestimable value of orchards recently planted in Sicilian rural areas. Nutrients (2017) 9(5):525.10.3390/nu905052528531110
312. Xu C, Zhang Y, Cao L, Lu J. Phenolic compounds and antioxidant properties of different grape cultivars grown in China. Food Chem (2010) 119:1557s–65s.10.1016/j.foodchem.2009.09.042
313. Ismail AM, Heuer S, Thomson MJ, Wissuwa M. Genetic and genomic approaches to develop rice germplasm for problem soils. Plant Soil (2007) 65:547–70.10.1007/s11103-007-9215-217703278
314. Wissuwa M, Ae N. Genotypic variation for tolerance to phosphorus deficiency in rice and the potential for its exploitation in rice improvement. Plant Breed (2001) 120:43–8.10.1046/j.1439-0523.2001.00561.x
315. White JG, Zasoski RJ. Mapping soil micronutrients. Field Crops Res (1999) 60:11–26.10.1016/S0378-4290(98)00130-0
316. Frossard E, Bucher M, Machler F, Mozafar A, Hurrell R. Potential for increasing the content and bioavailability of Fe, Zn and Ca in plants for human nutrition. J Sci Food Agric (2000) 80:861–79.10.1002/(SICI)1097-0010(20000515)80:7<861::AID-JSFA601>3.0.CO;2-P
317. Waters BM, Sankaran RP. Moving micronutrients from the soil to the seeds: genes and physiological processes from a biofortification perspective. Plant Sci (2011) 180(4):562–74.10.1016/j.plantsci.2010.12.00321421405
318. Lyons G, Ortiz-Monasterio I, Stangoulis J, Graham R. Selenium concentration in wheat grain: is there sufficient genotypic variation to use in breeding? Plant Soil (2005) 269(1):369–80.10.1007/s11104-004-0909-9
319. Oliva ML, Shannon JG, Sleper DA, Ellersieck MR, Cardinal AJ, Paris RL, et al. Stability of fatty acid profile in soybean genotypes with modified seed oil composition. Crop Sci (2006) 46:2069–75.10.2135/cropsci2005.12.0474
320. Al-Babili S, Beyer P. Golden rice on the road-five years to go? Trends Plant Sci (2004) 10(12):565–73.10.1016/j.tplants.2005.10.006
321. Inaba M, Macer D. Policy, regulation and attitudes towards agricultural biotechnology in Japan. J Int Biotechnol Laws (2004) 1(2):45–53.10.1515/jibl.2004.1.2.45
322. Watanabe KN, Sassa Y, Suda E, Chen CH, Inaba M, Kikuchi A. Global political, economic, social and technological issues on transgenic crops—review. Plant Biotechnol J (2005) 22(5):515–22.10.5511/plantbiotechnology.22.515
323. Welch RM. Effects of nutrient deficiencies on seed production and quality. Adv Plant Nutr (1986) 2:205–47.
324. Welch RM, Shuman L. Micronutrient nutrition of plants. Crit Rev Plant Sci (1995) 14(1):49–82.10.1080/07352689509701922
325. Haas JD, Beard JL, Murray-Kolb LE, del Mundo AM, Felix A, Gregorio GB. Iron-biofortified rice improves the iron stores of non-anemic Filipino women. J Nutr (2005) 135:2823–30.10.1093/jn/135.12.2823