RNAi-Mediated suppression of DET1 alters the levels of carotenoids and sinapate esters in seeds of brassica napus

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 12, 2009, Pages 5326-5333

  Wei, S H U ^a   Xiang, L I ^a   Gruber, Margaret Y ^a   Rong, L I ^a,b   Zhou, Rong ^a   Zebarjadi, Alireza ^a,c   Hannoufa, Abdelali ^a,d  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c Department of Plant Breeding ^*

^d RAZI UNIVERSITY   (Iran)

Author keywords

Brassica napus; Carotenoid; DET1; RNAi; Seed quality; Sinapate ester

Indexed keywords

CAROTENOID; COUMARIC ACID; ESTER; SINAPIC ACID; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; GENETICS; METABOLISM; PLANT SEED; RAPESEED; RNA INTERFERENCE;

BRASSICA NAPUS; CAROTENOIDS; COUMARIC ACIDS; ESTERS; PLANT PROTEINS; RNA INTERFERENCE; SEEDS;

BRASSICA NAPUS; LYCOPERSICON ESCULENTUM;

EID: 67649227310     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf803983w     Document Type: Article

References (34)

1. Cooper, D. A. Carotenoids in health and disease: recent scientific evaluations, research recommendations and the consumer. J. Nutr. 2004, 134 (1), 221S-224S.
2. Rao, A. V.; Rao, L. G. Carotenoids and human health. Pharmacol. Res. 2007, 55 (3), 207-216.
3. Howitt, C. A.; Pogson, B. J. Carotenoid accumulation and function in seed and non-green tissues. Plant Cell Environ. 2006, 29 (3), 435-445.
4. Giuliano, G.; Tavazza, R.; Diretto, G.; Beyer, P.; Taylor, M. A. Metabolic engineering of carotenoid biosynthesis in plants. Trends Biotechnol. 2008, 26 (3), 139-145.
5. Galpaz, N.; Ronen, G.; Khalfa, Z.; Zamir, D.; Hirschberg, J. A chromoplast-specific carotenoid biosynthesis pathway is revealed by cloning of the tomato white-flower locus. Plant Cell 2006, 18 (8), 1947-1960.
6. Ye, X.; Al-Babili, S.; Kloti, A.; Zhang, J.; Lucca, P.; Beyer, P.; Potrykus, I. Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 2000, 287 (5451), 303-305.
7. Shewmaker, C. K.; Sheey, J. A.; Daley, M.; Colburn, S.; Ke, D. Y. Seed-specific overexpression of phytoene synthase: increase in carotenoids and other metabolic effects. Plant J. 1999, 20 (4), 401-412.
8. Ducreux, L. J. M.; Morris, W. L.; Hedley, P. E.; Shepherd, T.; Davies, H. V.; Millam, S.; Taylor, M. A. Metabolic engineering of high carotenoid potato tubers containing enhanced levels of β-carotene and lutein. J. Exp. Bot. 2005, 56 (409), 81-89.
9. Lindgren, L.; Stahlberg, K. G.; Hoglund, A. S. Seed-specific overexpression of an endogenous Arabidopsis phytoene synthase gene results in delayed germination and increased levels of carotenoids, chlorophyll, and abscisic acid. Plant Physiol. 2003,132 (2), 779-785.
10. Rosati, C.; Aquilani, R.; Dharmapuri, S.; Pallara, P.; Marusic, C.; Tavazza, R.; Bouvier, F.; Camara, B.; Giuliano, G. Metabolic engineering of β-carotene and lycopene content in tomato fruit. Plant J. 2000, 24 (3), 413-419.
11. Diretto, G.; Tavazza, R.; Welsch, R.; Pizzichini, D.; Mourgues, F.; Papacchioli, V.; Beyer, P.; Giuliano, G. Metabolic engineering of potato tuber carotenoids through tuberspecific silencing of lycopene ε cyclase. BMC Plant Biol. 2006, 6, 13.
12. Yu, B.; Lydiate, D. J.; Schäfer, U. A.; Hannoufa, A. Characterization of a β-carotene hydroxylase of Adonis aestivalis and its expression in Arabidopsis thaliana. Planta 2007, 226 (1), 181-192.
13. Davuluri, G. R.; van Tuinen, A.; Fraser, P. D.; Manfredonia, A.; Newman, R.; Burgess, D.; Brummell, D. A.; King, S. R.; Palys, J.; Uhlig, J.; Bramley, P. M.; Pennings, H. M. J.; Bowler, C. Fruitspecific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes. Nat. Biotechnol. 2005, 23 (7), 890-895.
14. Bernhardt, A.; Lechner, E.; Hano, P.; Schade, V.; Dieterle, M.; Anders, M.; Dubin, M. J.; Benvenuto, G.; Bowler, C.; Genschik, P.; Hellmann, H. CUL4 associates with DDB1 and DET1 and its downregulation affects diverse aspects of development in Arabidopsis thaliana. Plant J. 2006, 47 (4), 591-603.
15. Chen, H.; Shen, Y.; Tang, X.; Yu, L.; Wang, J.; Guo, L.; Zhang, Y.; Zhang, H.; Feng, S.; Strickland, E.; Zheng, N.; Deng, X. W. Arabidopsis CULLIN4 forms an E3 ubiquitin ligase with RBX1 and the CDD complex in mediating light control of development. Plant Cell 1006, 18 (8), 1991-2004.
16. Schäfer, E.; Bowler, C. Phytochrome-mediated photoperception and signal transduction in higher plants. EMBO Rep. 2002, 3(11), 10421048.
17. Schroeder, D. F.; Gahrtz, M.; Maxwell, B. B.; Cook, R. K.; Kan, J. M.; Alonso, J. M.; Ecker, J. R.; Chory, J. De-etiolated 1 (DET1) and damaged DNA binding protein 1 (DDB1) interact to regulate Arabidopsis photomorphogenesis. Curr. Biol. 2002, 12 (17), 14621472.
18. Ismail, F.; Vaisey-Genser, M.; Fyfe, B. Bitterness and astringency of sinapine and its components. J. Food Sci. 1981, 46 (4), 12411244.
19. Bhinu, V. S.; Schäfer, U. A.; Li, R.; Huang, J.; Hannoufa, A. Targeted modulation of sinapine biosynthesis pathway for seed quality improvement in Brassica napus. Transgenic Res. 2008, doi 10.1007/s11248-008-9194-3.
20. Baumert, A.; Milkowski, C.; Schmidt, J.; Nimtz, M.; Wray, V.; Strack, D. Formation of a complex pattern of sinapate esters in Brassica napus seeds, catalyzed by enzymes of a serine carboxypeptidase-like acyltransferase family?. Phytochemistry 2005, 66 (11), 1334-1345.
21. Milkowski, C.; Baumert, A.; Schmidt, D.; Nehlin, L.; Strack, D. Molecular regulation of sinapate ester metabolism in Brassica napus: expression of genes, properties of the encoded proteins and correlation of enzyme activities with metabolite accumulation. Plant J. 2004, 38 (1), 80-92.
22. Rask, L.; Ellerstrom, M.; Ezcurra, I.; Stalberg, K.; Wycliffe, P. Seedspecific regulation of the napin promoter in Brassica napus. J. Plant Physiol. 1998,152 (6), 595-599.
23. Moloney, M. M.; Walker, J. M.; Sharma, K. K. High efficiency transformation of Brassica napus, using Agrobacterium vectors. Plant Cell Rep. 1989, 8 (4), 238-242.
24. Shi, H.; Bressan, R. RNA extraction. In Arabidopsis Protocols, 2nd ed.; Salinas, J., Jose, J., Sanchez-Serrano, J. J., Eds.; Humana Press: Totowa, NJ, 2006; pp 345-348.
25. Ferreres, F .; Sousa, C.; Valentão, P.; Seabra, R. M.; Pereira, J. A.; Andrade, P.B. Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phytochemical characterization and antioxidant potential. Food Chem. 2007, 101 (2), 549-558.
26. Ronen, G.; Merav Cohen, M.; Dani Zamir, D.; Hirschberg, J. Regulation of carotenoid biosynthesis during tomato fruit development: expression of the gene for lycopene ε-cyclase is down-regulated during ripening and is elevated in the mutant Delta. Plant J. 1999, 17 (4), 341-351.
27. Fraser, C. M.; Thompson, M. G.; Shirley, A. M.; Ralph, J.; Schoenherr, J. A.; Sinlapadech, T.; Hall, M. C.; Chapple, C. Related Arabidopsis serine carboxypeptidase-like sinapoylglucose acyltransferases display distinct but overlapping substrate specificities. Plant Physiol. 2007, 144 (4), 1986-1999.
28. Stehle, F.; Brandt, W.; Schmidt, J.; Milkowski, C.; Strack, D. Activities of Arabidopsis sinapoylglucose: malate sinapoyltransferase shed light on functional diversification of serine carboxypeptidase-like acyltransferases. Phytochemistry 2008, 69 (9), 1826-1831.
29. Chory, J.; Peto, C.; Feinbaum, R.; Pratt, L.; Ausubel, F. Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light. Cell. 1989, 58 (5), 991-999.
30. Pepper, A.; Chory, J. Extragenic suppressors of the Arabidopsis detl mutant identify elements of flowering-time and light-response regulatory pathways. Genetics 1997, 145 (4), 1125-1137.
31. Chory, J.; Peto, G Mutations in the DET1 gene affect cell-typespecific expression of light regulated genes and chloroplast development in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 1990, 87 (22), 8776-8780.
32. Mustilli, A. C.; Fenzi, F.; Ciliento, R.; Alfano, F.; Bowler, C. Phenotype of the tomato high pigment-2 mutant is caused by a mutation in the tomato homolog of DEETIOLATED1. Plant Cell 1999, 11 (2), 145-157.
33. Davuluri, G. R.; van Tuinen, A.; Mustilli, A. C.; Manfredonia, A.; Newman, R.; Burgess, D.; Brummell, D. A.; King, S. R.; Palys, J.; Uhlig, J.; Pennings, H. M. J.; Bowler, C. Manipulation of DET1 expression in tomato results in photomorphogenic phenotypes caused by post-transcriptional gene silencing. Plant J. 2004, 40 (7), 344-354.
34. Hu, J.; Aguirre, M.; Peto, C.; Alonso, J.; Ecker, J; Chory, J. A role for peroxisomes in photomorphogenesis and development of Arabidopsis. Science 2002, 297 (5580), 405-409.