Silencing of GmFAD3 gene by siRNA leads to low α-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]

Transgenic Research

Volumn 17, Issue 5, 2008, Pages 839-850

  Flores, Teresita ^a,b   Karpova, Olga ^a   Su, Xiujuan ^a   Zeng, Peiyu ^a,c   Bilyeu, Kristin ^a   Sleper, David A ^a   Nguyen, Henry T ^a   Zhang, Zhanyuan J ^a  

^a UNIVERSITY OF MISSOURI   (United States)

^b University of Missouri   (United States)

^c Center for Agriculture and Natural Resources   (United States)

Author keywords

Gene silencing; GmFAD3; Inverted repeat; RNAi; siRNA

Indexed keywords

LINOLENIC ACID; SMALL INTERFERING RNA;

ARTICLE; GENE; GENE SILENCING; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY; SOYBEAN; TRANSGENIC PLANT;

ALPHA-LINOLENIC ACID; BASE SEQUENCE; GENE SILENCING; GENES, PLANT; MOLECULAR SEQUENCE DATA; MUTATION; PLANTS, GENETICALLY MODIFIED; RNA, SMALL INTERFERING; SEQUENCE HOMOLOGY, NUCLEIC ACID; SOYBEANS;

GLYCINE MAX;

EID: 50849105419     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-008-9167-6     Document Type: Article

References (43)

1. D Baulcombe 2004 RNA silencing in plants Nature 431 356 363
2. K Bilyeu DA Palavalli DA Sleper PR Beuselinck 2003 Three microsomal omega-3 fatty-acid desaturase genes contribute to soybean linolenic acid levels Crop Sci 43 1833 1838
3. K Bilyeu L Palavalli DA Sleper PR Beuselinck 2006 Molecular genetic resources for development of 1 percent linolenic acid soybeans Crop Sci 46 1913 1918
4. P Brodersen O Voinnet 2006 The diversity of RNA silencing pathways in plants Trends Genet 23 268 280
5. EB Cahoon EF Marrillia KL Stecca SE Hall DC Taylor AJ Kinney 2000 Production of fatty acid components of meadowfoam oil in somatic soybean embryos Plant Physiol 124 243 251
6. JC Carrington DD Freed CS Oh 1990 Expression of potyviral polyproteins in transgenic plants reveals three proteolytic activities required for complete processing EMBO J 9 1347 1353
7. TJ Cho CS Davies NC Nielsen 1989 Inheritance and organization of glycinin genes in soybean Plant Cell 1 329 337
8. C Dean J Jones M Faureau P Dunsmuir J Bedbrook 1988 Influence of flanking sequences on variability in expression levels of an induced gene in transgenic tobacco plants Nucl Acids Res 16 926 9283
9. SL Dellaporta J Wood JB Hicks 1983 A plant DNA minipreparation: version 2 Plant Mol Biol Rep 1 19 22
10. Der G, Everitt B (2001) A handbook of statistical analyses using SAS, 2nd edn. CRC Press
11. HJ Dutton CJ Lancaster CD Evans JC Cowan 1951 The flavor problem of soybean oil. VIII. Linolenic acid J Am Oil Chem Soc 28 115 118
12. WR Fehr GA Welke EG Hammond DN Duvick SR Cianzio 1992 Inheritance of reduced linolenic acid content in soybean genotypes A16 and A17 Crop Sci 32 903 906
13. B Frame H Shou R Chikwamba Z Zhang C Xiang T Fonger S.E Pegg B Li D Nettleton D Pei K Wang 2002 Agrobacterium-mediated transformation of maize embryos using a standard binary vector system Plant Physiol 129 13 22
14. AJ Hamilton DC Baulcombe 1999 A species of small antisense RNA in posttranscriptional gene silencing in plants Science 286 950 952
15. S Hirai S Oka E Adachi H Kodoma 2007 The effects of spacer sequences on silencing efficiency of plant RNAi vectors Plant Cell Rep 26 651 659
16. AL Jackson SR Bartz J Schelter SV Kobayashi J Burchard M Mao B Li G Cavet PS Linsley 2003 Expression profiling reveals off-target gene regulation by RNAi Nat Biotechnol 21 635 637
17. A Jadhav V Katavic E-F Marillia EM Giblin DL Barton A Kumar C Sonntag V Babic WA Keller DC Taylor 2005 Increased levels of erucic acid in Brassica carinata by co-suppression and antisense repression of the endogenous FAD2 gene Metab Eng 7 215 220
18. MW Jones-Rhoades DP Bartel B Bartel 2006 MicroRNAs and their regulatory roles in plants Annu Rev Plant Biol 57 19 53
19. AJ Kinney 1996 Designer oils for better nutrition Nat Biotechnol 14 946
20. Kinney AJ, Knowlton S (1998) Designer oils: the high oleic acid soybean. In: Roller S, Harlander S (eds) Genetic modification in the food industry: a strategy for food quality improvement. Chapman & Hall, London, pp 192-213
21. Q Liu SP Singh G Allan 2002 Greenhigh-stearic and high-oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing Plant Physiol 129 1732 1743
22. C Llave Z Xie KD Kasschau JC Carrington 2002 Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA Science 297 2053 2056
23. M Matzke AJM Matzke 1993 Genomic imprinting in plants. Parental effect and trans-inactivation phenomena Annu Rev Plant Physiol Plant Mol Biol 44 53 76
24. AC Nunes GR Vianna F Cuneo J Amaya-Farfan G de Capdeville EL Rech FJ Aragao 2006 RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content Planta 234 125 132
25. S Ogita H Uefuji Y Yamaguchi N Koizumi H Sano 2003 Producing decaffeinated coffee plants Nature 423 823
26. J Ohlrogge J Browse 1995 Lipid biosynthesis Plant Cell 7 957 970
27. PM Olhoft LE Flagel CM Donovan DA Somers 2003 Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method Planta 216 723 735
28. C Peach J Velten 1991 Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters Plant Mol Biol 17 49 60
29. S Rahman T Kinoshita T Anai S Arima Y Takagi 1998 Genetic relationships of soybean mutants for different linolenic acid contents Crop Sci 38 702 706
30. SA Ruuska T Girke C Benning JB Ohlrogge 2002 Contrapuntal network of gene expression during Arabidopsis seed filling Plant Cell 14 1191 1206
31. RC Shoemaker K Polzin J Labate J Specht EC Brummer T Olson N Young V Concibido J Wilcox JP Tamulonis G Kochert HR Boerma 1996 Genome duplication in soybean (Glycine subgenus soja) Genetics 144 329 338
32. RC Shoemaker J Schlueter JJ Doyle 2006 Paleopolyploidy and gene duplication in soybean and other legumes Curr Opin Plant Biol 9 104 109
33. TL Sims RB Goldberg 1989 The glycinin Gy1 gene from soybean Nucleic Acids Res 17 4386
34. NA Smith SP Singh MB Wang PA Stoutjesdijk AG Green PM Waterhouse 2000 Total silencing by intron-spliced hairpin RNAs Nature 407 319 320
35. I Sivaraman N Arumugam YS Sodhi V Gupta A Mukhopadhyay AK Pradhan PK Kumar Burma D Pental 2004 Development of high oleic and low linoleic acid transgenics in a zero erucic acid Brassica juncea L. (Indian mustard) line by antisense suppression of the fad2 gene Mol Breed 13 365 375
36. EM Southern 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis J Mol Biol 98 503 517
37. S Subramanian MY Graham O Yu TL Graham 2005 RNA Interference of soybean isoflavone synthase genes leads to silencing in tissues distal to the transformation site and to enhanced susceptibility to Phytophthora sojae Plant Physiol 137 1345 1353
38. G Tang G Galili 2004 Using RNAi to improve plant nutritional value: from mechanism to application Trends Biotechnol 22 463 469
39. H Vaucheret 2006 Post-transcriptional small RNA pathways in plants: mechanisms and regulations Genes Dev 20 759 771
40. JM Watson AF Fusaro M Wang PM Waterhouse 2005 RNA silencing platforms in plants FEBS Lett 579 5982 5987
41. Z. Zhang D.P. Coyne A.K. Vidaver A Mitra 1998 Expression of human lactoferrin cDNA confers resistance to Ralstonia solanacearum in transgenic tobacco plants Phytopathol 88 730 734
42. Z Zhang A Xing PE Staswick TE Clemente 1999 The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean Plant Cell Tiss Organ Cult 56 37 46
43. P Zeng D Vadnais Z Zhang J Polacco 2004 Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merr.] Plant Cell Rep 22 478 482