Transgenic sorghum with improved digestibility of storage proteins obtained by Agrobacterium-mediated transformation

Russian Journal of Plant Physiology

Volumn 63, Issue 5, 2016, Pages 678-689

  Elkonin, L A ^a   Italianskaya, J V ^a   Domanina, I V ^a   Selivanov, N Yu ^b   Rakitin, A L ^c   Ravin, N V ^c  

^a Agricultural Research Institute for Southeastern Regions   (Russian Federation)

^b INSTITUTE OF BIOCHEMISTRY AND PHYSIOLOGY OF PLANTS AND MICROORGANISMS   (Russian Federation)

^c INSTITUTE OF BIOENGINEERING   (Russian Federation)

Author keywords

Agrobacterium mediated genetic transformation; endosperm; gamma kafirin; in vitro protein digestibility; RNA silencing; Sorghum bicolor; transgenic plants

Indexed keywords

EID: 84981736319     PISSN: 10214437     EISSN: None     Source Type: Journal    
DOI: 10.1134/S1021443716050046     Document Type: Article

References (37)

1. Shewry, P.R., Improving the protein content and composition of cereal grain, J. Cereal Sci., 2007, vol. 46, pp. 239–250.
2. Godwin, I.D., Williams, S.B., Pandit, P.S., and Laidlaw, H.K.C., Multifunctional grains for the future: genetic engineering for enhanced and novel cereal quality, In Vitro Cell. Dev. Biol.–Plant, 2009, vol. 45, pp. 383–399.
3. Segal, G., Song, R., and Messing, J., A new opaque variant of maize by a single dominant RNA-interference-inducing transgene, Genetics, 2003, vol. 165, pp. 387–397.
4. Huang, S., Frizzi, A., Florida, C.A., Kruger, D.E., and Luethy, M.H., High lysine and high tryptophan transgenic maize resulting from the reduction of both 19- and 22-kD α-zeins, Plant Mol. Biol., 2006, vol. 61, pp. 525–535.
5. Wu, Y., Holding, D.R., and Messing, J., γ-Zeins are essential for endosperm modification in quality protein maize, Proc. Natl. Acad. Sci. USA, 2010, vol. 107, pp. 12810–12815.
6. Guo, X., Yuan, L., Chen, H., Sato, S.J., Clemente, T.E., and Holding, D.R., Non-redundant function of zeins and their correct stoichiometric ratio drive protein body formation in maize endosperm, Plant Physiol., 2013, vol. 162, pp. 1359–1369.
7. Belton, P.S., Delgadillo, I., Halford, N.G., and Shewry, P.R., Kafirin structure and functionality, J. Cereal Sci., 2006, vol. 44, pp. 272–286.
8. De Mesa-Stonestreet, N.J., Alavi, S., and Bean, S.R., Sorghum proteins: the concentration, isolation, modification, and food applications of kafirins, J. Food Sci., 2010, vol. 75, pp. 90–104.
9. Oria, M.P., Hamaker, B.R., and Shull, J.M., Resistance of sorghum α-, β- and γ-kafirins to pepsin digestion, J. Agric. Food Chem., 1995, vol. 43, pp. 2148–2153.
10. Oria, M.P., Hamaker, B.R., Axtell, J.D., and Huang, C.P., A highly digestible sorghum mutant cultivar exhibits a unique folded structure of endosperm protein bodies, Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 5065–5070.
11. Howe, A., Sato, S., Dweikat, I., Fromm, M., and Clemente, T., Rapid and reproducible Agrobacteriummediated transformation of sorghum, Plant Cell Rep., 2006, vol. 25, pp. 784–791.
12. Wu, E., Lenderts, B., Glassman, K., Berezowska-Kaniewska, M., Christensen, H., Asmus, T., Zhen, S., Chu, U., Cho, M.J., and Zhao, Z.Y., Optimized Agrobacterium-mediated sorghum transformation protocol and molecular data of transgenic sorghum plants, InVitro Cell. Dev. Biol.–Plant, 2014, vol. 50, pp. 9–18.
13. Katoch, R. and Thakur, N., Advances in RNA interference technology and its impact on nutritional improvement, disease and insect control in plants, Appl. Biochem. Biotechnol., 2013, vol. 169, pp. 1579–1605.
14. Younis, A., Siddique, M.I., Kim, C.K., and Lim, K.B., RNA interference (RNAi) induced gene silencing: a promising approach of hi-tech plant breeding, Int. J. Biol. Sci., 2014, vol. 10, pp. 1150–1158.
15. Da Silva, L.S., Taylor, J., and Taylor, J.R., Transgenic sorghum with altered kafirin synthesis: kafirin solubility, polymerization, and protein digestion, J. Agric. Food Chem., 2011, vol. 59, pp. 9265–9270.
16. Da Silva, L.S., Jung, R., Zhao, Z., Glassman, K., Grootboom, A.W., Mehlo, L., O’Kennedy, M.M., Taylor, J., and Taylor, J.R.N., Effect of suppressing the synthesis of different kafirin subclasses on grain endosperm texture, protein body structure and protein nutritional quality in improved sorghum lines, J. Cereal Sci., 2011, vol. 54, pp. 160–167.
17. Kumar, T., Dweikat, I., Sato, S., Ge, Z., Nersesian, N., Chen, H., Elthon, T., Bean, S., Ioerger, B.P., and Tilley, M., Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench), Plant Biotechnol. J., 2012, vol. 10, pp. 533–544.
18. Grootboom, A.W., Mkhonza, N.L., Mbambo, Z., O’Kennedy, M.M., Silva, L.S., Taylor, J., Taylor, J.R.N., Chikwamba, R., and Mehlo, L., Co-suppression of synthesis of major α-kafirin sub-class together with γ-kafirin-1 and γ-kafirin-2 required for substantially improved protein digestibility in transgenic sorghum, Plant Cell Rep., 2014, vol. 33, pp. 521–537.
19. De Barros, E.G., Takasaki, K., Kirleis, A.W., and Larkins, B.A., Nucleotide sequence of a cDNA clone encoding γ-kafirin protein from Sorghum bicolor, Plant Physiol., 1991, vol. 97, pp. 1606–1607.
20. Gelvin, S.B., Agrobacterium virulence gene induction, Agrobacterium Protocols, vol. 1, Ser. Methods in Molecular Biology, vol. 343, Wang, K., Ed., Totowa, NJ: Humana Press Inc., 2006, P. 77–84.
21. Chilton, M.-D., Currier, T.C., Farrand, S.K., Bendich, A.J., Gordon, M.P., and Nester, E.W., Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors, Proc. Natl. Acad. Sci. USA, 1974, vol. 71, pp. 3672–3676.
22. Zhao, Z.Y., Cai, T.S., Tagliani, L., Miller, M., Wang, N., Pang, H., Rudert, M., Schroeder, S., Hondred, D., Seltzer, J., and Pierce, D., Agrobacterium-mediated sorghum transformation, Plant Mol. Biol., 2000, vol. 44, pp. 789–798.
23. Elkonin, L.A. and Pakhomova, N.V., Influence of nitrogen and phosphorus on induction embryogenic callus of sorghum, Plant Cell Tissue Organ Cult., 2000, vol. 61, pp. 115–123.
24. Doyle, J.J. and Doyle, J.L., A rapid total DNA preparation procedure for fresh plant tissue, Focus, 1990, vol. 12, pp. 13–15.
25. Weaver, C.A., Hamaker, B.R., and Axtell, J.D., Discovery of grain sorghum germplasm with high uncooked and cooked in vitro protein digestibility, Cereal Chem., 1998, vol. 75, pp. 665–670.
26. Nunes, A., Correia, I., Barros, A., and Delgadillo, I., Sequential in vitro pepsin digestion of uncooked and cooked sorghum and maize samples, J. Agric. Food Chem., 2004, vol. 52, pp. 2052–2058.
27. Elkonin, L.A., Italianskaya, J.V., Fadeeva, I.Yu., Bychkova, V.V., and Kozhemyakin, V.V., In vitro protein digestibility in grain sorghum: effect of genotype and interaction with starch digestibility, Euphytica, 2013, vol. 193, pp. 327–337.
28. Aboubacar, A., Axtell, J.D., Huang, C.P., and Hamaker, B.R., A rapid protein digestibility assay for identifying highly digestible sorghum lines, Cereal Chem., 2001, vol. 78, pp. 160–165.
29. Wong, J.H., Marx, D.B., Wilson, J.D., Buchanan, B.B., Lemaux, P.G., and Pedersen, J.F., Principal component analysis and biochemical characterization of protein and starch reveal primary targets for improving sorghum grain, Plant Sci., 2010, vol. 179, pp. 598–611.
30. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.
31. Emani, C., Sunilkumar, G., and Rathore, K.S., Transgene silencing and reactivation in sorghum, Plant Sci., 2002, vol. 162, pp. 181–192.
32. Shull, J.M., Watterson, J.J., and Kirleis, A.W., Proposed nomenclature for the alcohol soluble proteins (kafirins) of Sorghum bicolor (L. Moench) based on molecular weight, solubility and structure, J. Agric. Food Chem., 1991, vol. 39, pp. 83–87.
33. Shull, J.M., Watterson, J.J., and Kirleis, A.W., Purification and immunocytochemical localization of kafirins in Sorghum bicolor (L. Moench) endosperm, Protoplasma, 1992, vol. 171, pp. 64–74.
34. Wu, Y., Yuan, L., Guo, X., Holding, D.R., and Messing, J., Mutation in the seed storage protein kafirin creates a high-value food trait in sorghum, Nat. Commun., 2013, vol. 4, p.2217. doi: doi 10.1038/ncoмMs3217
35. De Freitas, F., Yunes, J.A., da Silva, M.J., Arruda, P., and Leite, A., Structural characterization and promoter activity analysis of the γ-kafirin gene from sorghum, Mol. Gen. Genet., 1994, vol. 245, pp. 177–186.
36. Italianskaya, J.V., Elkonin, L.A., and Kozhemyakin, V.V., Characterization of kafirin composition and in vitro digestibility in CMS-lines, fertility restorers and F1 hybrids with the new types of CMS-inducing cytoplasms of sorghum, Plant Breed., 2009, vol. 128, pp. 624–630.
37. Tesso, T., Ejeta, G., Chandrashekar, A., Huang, C.P., Tandjung, A., Lewamy, M., Axtell, J., and Hamaker, B.R., A novel modified endosperm texture in a mutant high-protein digestibility/high-lysine grain sorghum (Sorghum bicolor (L.) Moench), Cereal Chem., 2006, vol. 83, pp. 194–201.