High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile

Journal of Experimental Botany

Volumn 57, Issue 12, 2006, Pages 3069-3078

  Wakasa, Kyo ^a,b,e   Hasegawa, Hisakazu ^c   Nemoto, Hiroshi ^a   Matsuda, Fumio ^b   Miyazawa, Haruna ^b,d   Tozawa, Yuzuru ^a,b   Morino, Keiko ^b,d   Komatsu, Akira ^a,b   Yamada, Tetsuya ^b   Terakawa, Teruhiko ^c   Miyagawa, Hisashi ^b,d  

^a NATIONAL INSTITUTE OF CROP SCIENCE   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c Hokko Chemical Industry Co Ltd ^*  (Japan)

^d KYOTO UNIVERSITY   (Japan)

^e TOKYO UNIVERSITY OF AGRICULTURE   (Japan)

Author keywords

5MT; Amino acids; Anthranilate synthase; AS; IAA; Isolated field; Non containment greenhouse; OASA1D; Oryza sativa

Indexed keywords

ANTHRANILATE SYNTHASE (AS); ISOLATED FIELDS; NON-CONTAINMENT GREENHOUSES; ORYZA SATIVA; TRANSGENIC PLANTS;

AGRONOMY; AMINO ACIDS; BIOTECHNOLOGY; CROPS; ENZYME IMMOBILIZATION; METABOLITES; PLANTS (BOTANY);

SEED;

ANTHRANILATE SYNTHASE; INDOLEACETIC ACID; INDOLEACETIC ACID DERIVATIVE; PHENOL DERIVATIVE; PROTEIN SUBUNIT; TRYPTOPHAN;

ARTICLE; GENETIC ENGINEERING; GENETICS; GERMINATION; GROWTH, DEVELOPMENT AND AGING; HOMOZYGOTE; METABOLISM; PHYSIOLOGY; PLANT SEED; PRENATAL DEVELOPMENT; RICE; TRANSGENE; TRANSGENIC PLANT;

ANTHRANILATE SYNTHASE; GENETIC ENGINEERING; GERMINATION; HOMOZYGOTE; INDOLEACETIC ACIDS; ORYZA SATIVA; PHENOLS; PLANTS, GENETICALLY MODIFIED; PROTEIN SUBUNITS; SEEDS; TRANSGENES; TRYPTOPHAN;

AMINO ACIDS; BIOTECHNOLOGY; CALLUS; FEEDBACK; INDOLEACETIC ACID; LEAVES; PLANTS; RICE; TRYPTOPHAN;

ANIMALIA; ORYZA SATIVA;

EID: 33749003999     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/erl068     Document Type: Article

References (43)

1. Anzala F, Paven MM, Fournier S, Rondeau D, Limami AM. 2006. Physiological and molecular aspects of aspartate-derived amino acid metabolism during germination and post-germination growth in two maize genotypes differing in germination efficiency. Journal of Experimental Botany 57, 645-653.
2. Bender J, Fink GR. 1998. A myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis. Proceedings of the National Academy of Sciences, USA 95, 5655-5660.
3. Bandurski RS, Schulze A. 1977. Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiology 60, 211-213.
4. Chavadej S, Brisson N, McNeil JN, De Luca V. 1994. Redirection of tryptophan leads to production of low indole glucosinolate canola. Proceedings of the National Academy of Sciences, USA 91, 2166-2170.
5. Cho H-J, Brotherton JE, Song H-S, Widholm JM. 2000. Increasing tryptophan synthesis in a forage legume Astragalus sinicus by expressing the tobacco feedback-insensitive anthranilate synthase (ASA2) gene. Plant Physiology 123, 1069-1076.
6. DellaPenna D. 2001. Plant metabolic engineering. Plant Physiology 125, 160-163.
7. Eder K, Peganova S, Kluge H. 2001. Studies on the tryptophan requirement of piglets. Archiv für Tierenähung 55, 281-297.
8. Falco SC, Guida T, Locke M, Mauvais J, Sanders C, Ward RT, Webber P. 1995. Transgenic canola and soybean seeds with increased lysine. Biotechnology 13, 577-582.
9. Henry Y, Sève B, Colléaux Y, Ganier P, Saligaut C, Jégo P. 1992. Interactive effects of dietary levels of tryptophan and protein on voluntary feed intake and growth performance in pigs, in relation to plasma free amino acids and hypothalamic serotonin. Journal of Animal Science 70, 1873-1887.
10. Hiei Y, Ohta S, Komari T, Kumashiro T. 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal 6, 271-282.
11. Ibaraki Prefecture Standard Lowland Rice Cultivation. 1990. In: Standard cultivation of crops in Ibaraki Prefecture. Ibaraki Prefecture, 1-12.
12. Kreps JA, Town CD. 1992. Isolation and characterization of a mutant of Arabidopsis thaliana resistant to α-methyltryptophan. Plant Physiology 99, 269-275.
13. Lai J, Messing J. 2002. Increasing maize seed methionine by mRNA stability. The Plant Journal 30, 395-402.
14. Limami AM, Rouillon C, Glevarec G, Gallais A, Hirel B. 2002. Genetic and physiological analysis of germination efficiency in maize in relation to nitrogen metabolism reveals the importance of cytosolic glutamine synthetase. Plant Physiology 130, 1860-1870.
15. Ludwig-Muller J, Sass S, Sutter EG, Wodner M, Epstein E. 1993. Indole-3-butyric acid in Arabidopsis thaliana. I. Identification and quantification. Plant Growth Regulation 13, 179-187.
16. Massey KA, Blakeslee CH, Pitkow HP. 1998. A review of physiological and metabolic effects of essential amino acids. Amino Acids 14, 271-300.
17. Matsuda F, Miyazawa H, Wakasa K, Miyagawa H. 2005. Quantification of indole-3-acetic acid and amino acid conjugates in rice by liquid chromatography-electrospray ionization-tandem mass spectrometry. Bioscience, Biotechnology, and Biochemistry 69, 778-783.
18. Mazur B, Krebbers E, Tingey S. 1999. Gene discovery and product development for grain quality traits. Science 285, 372-375.
19. Morandini P, Salamini F. 2003. Plant biotechnology and breeding: allied for years to come. Trends in Plant Science 8, 70-75.
20. Morino K, Matsuda F, Miyazawa H, Sukegawa A, Miyagawa M, Wakasa K. 2005. Metabolic profiling of tryptophan-overproducing rice calli that express a feedback-insensitive a subunit of anthranilate synthase. Plant and Cell Physiology 46, 514-521.
21. Morris CF, Mueller DD, Faubin JM, Paulsen GM. 1988. Identification of L-tryptophan as an endogenous inhibitor of embryo germination in white wheat. Plant Physiology 88, 435-440.
22. Movig L, Tabe LM, Eggum BO, Moore AE, Craig S, Spencer D, Higgins TJV. 1997. Enhanced methionine levels and increased nutritive value of seeds of transgenic lupins (Lupinus angustifolius L.) expressing a sunflower seed albumin gene. Proceedings of the National Academy of Sciences, USA 94, 8393-8398.
23. Phillips RL, Kaeppler SM, Olhoft P. 1994. Genetic instability of plant tissue cultures: Breakdown of normal controls. Proceedings of the National Academy of Sciences, USA 91, 5222-5226.
24. Ramaih S, Guedira M, Paulsen GM. 2003. Relationship of indole acetic acid and tryptophan to dormancy and preharvest sprouting of wheat. Functional Plant Biology 30, 939-945.
25. Sakai M, Iida S, Maeda H, Sunohara Y, Nemoto H, Imbe T. 2003a. New varieties for whole crop silage use in Japan. Breeding Science 53, 271-275.
26. Sakai M, Imbe T, Nemoto H, et al. 2003b. A new rice variety for whole-crop silage 'Kusahonami'. Bulletin of National Institute of Crop Science 4, 1-15.
27. Sève B. 1999. Physiological roles of tryptophan in pig nutrition. Advances in Experimental Medicine and Biology 467, 729-741.
28. Subcommittee on Poultry Nutrition, Committee on Animal Nutrition, Board on Agriculture, National Research Council. 1994. Nutrient requirements of chickens. In: Nutrient requirements of poultry, 9th edn. National Academy Press, 19-34.
29. Subcommittee on Swine Nutrition, Committee on Animal Nutrition, Board on Agriculture, National Research Council. 1998. Nutrient requirement tables. In: Nutrient requirements of swine, 10th edn. National Academy Press, 110-123.
30. Sung ZR. 1979. Relationships of indole-3-acetic acid and tryptophan concentrations in normal and 5-methyltryptophan-resistant cell lines of wild carrots. Planta 145, 339-345.
31. Takeoka Y, Shimizu M, Wada T. 1993. Panicles. In: Matsuo T, Hoshikawa K, eds. Science of the rice plant, Vol. 1. Morphology. Food and Agriculture Policy Research Center, 295-338.
32. Takimoto I, Christensen AH, Quail PH, Uchimiya H, Toki S. 1994. Non-systemic expression of a stress-responsive maize polyubiquitin gene (Ubi-1) in transgenic rice plants. Plant Molecular Biology 26, 1007-1012.
33. Tam YY, Slovin JP, Cohen JD. 1995. Selection and characterization of α-methyltryptophan-resistant lines of Lemna gibba showing a rapid rate of indole-3-acetic acid turnover. Plant Physiology 107, 77-85.
34. Tozawa Y, Hasegawa H, Terakawa T, Wakasa K. 2001. Characterization of rice anthranilate synthase α-subunit genes OASA1 and OASA2. Tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASA1. Plant Physiology 126, 1493-1506.
35. Urushibara S, Tozawa Y, Kawagishi-Kobayashi M, Wakasa K. 2001. Efficient transformation of suspension-cultured rice cells mediated by Agrobacterium tumefaciens. Breeding Science 51, 33-38.
36. Wakasa K, Widholm J. 1987. A 5-methyltryptophan resistant rice mutant, MTR1, selected in tissue culture. Theoretical and Applied Genetics 74, 49-54.
37. Wang X, Larkins A. 2001. Genetic analysis of amino acid accumulation in opaque-2 maize endosperm. Plant Physiology 125, 1766-1777.
38. Wang X, Stumpf DK, Larkins A. 2001. Aspartate kinase 2. A candidate gene of a quantitative trait locus influencing free amino acid content in maize endosperm. Plant Physiology 125, 1778-1787.
39. Widholm J. 1977. Relation between auxin autotrophy and tryptophan accumulation in cultured plant cells. Planta 134, 103-108.
40. Yamada T, Tozawa Y, Hasegawa H, Terakawa T, Ohkawa Y, Wakasa K. 2004. Use of a feedback-insensitive α subunit of anthranilate synthase as a selectable marker for transformation of rice and potato. Molecular Breeding 14, 363-373.
41. Yao K, De Luca V, Brisson N. 1995. Creation of a metabolic sink for tryptophan alters the phenylpropanoid pathway and the susceptibility of potato to Phytophthora infestans. The Plant Cell 7, 1787-1799.
42. Zhang X-H, Brotherton JE, Widholm JM, Portis Jr AR. 2001. Targeting a nuclear anthranilate synthase α-subunit gene to the tobacco plastid genome results in enhanced tryptophan biosynthesis. Return of a gene to its pre-endosymbiotic origin. Plant Physiology 127, 131-141.
43. Zhu X, Galili G. 2003. Increased lysine synthesis coupled with a knockout of its catabolism synergistically boosts lysine content and also transregulates the metabolism of other amino acids in Arabidopsis seeds. The Plant Cell 15, 845-853.