Modifying lysine biosynthesis and catabolism in corn with a single bifunctional expression/silencing transgene cassette

Plant Biotechnology Journal

Volumn 6, Issue 1, 2008, Pages 13-21

  Frizzi, Alessandra ^a   Huang, Shihshieh ^a   Gilbertson, Larry A ^a   Armstrong, Toni A ^a   Luethy, Michael H ^a   Malvar, Thomas M ^a  

^a MONSANTO COMPANY   (United States)

Author keywords

CordapA; Dihydrodipicolinate synthase (DHDPS); Double stranded RNA; High lysine corn; Intron embedded; Lysine ketoglutarate reductase saccharophine dehydrogenase (LKR SDH); Quality trait

Indexed keywords

COMPLEMENTARY DNA; DIHYDRODIPICOLINATE SYNTHASE; DOUBLE STRANDED RNA; HEAT SHOCK PROTEIN 70; HYDROLYASE; LYSINE; SACCHAROPINE DEHYDROGENASE; UNCLASSIFIED DRUG;

ARTICLE; BIOSYNTHESIS; CORYNEBACTERIUM GLUTAMICUM; ENZYMOLOGY; GENE EXPRESSION; GENE TARGETING; GENETICS; INTRON; MAIZE; METABOLISM; NUCLEOTIDE REPEAT; PLANT SEED; TRANSGENE; TRANSGENIC PLANT;

CORYNEBACTERIUM GLUTAMICUM; DNA, COMPLEMENTARY; GENE EXPRESSION; GENE TARGETING; HSP70 HEAT-SHOCK PROTEINS; HYDRO-LYASES; INTRONS; LYSINE; PLANTS, GENETICALLY MODIFIED; REPETITIVE SEQUENCES, NUCLEIC ACID; RNA, DOUBLE-STRANDED; SACCHAROPINE DEHYDROGENASES; SEEDS; TRANSGENES; ZEA MAYS;

ANIMALIA; ZEA MAYS;

EID: 36849093996     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/j.1467-7652.2007.00290.x     Document Type: Article

References (43)

1. Armstrong, C.L. Rout, J.R. (2001) A Novel Agrobacterium-Mediated Plant Transformation Method. St. Louis, MO : Monsanto Company.
2. Arruda, P., Kemper, E.L., Papes, F. Leite, A. (2000) Regulation of lysine catabolism in higher plants. Trends Plant Sci. 5, 324 330.
3. Azevedo, R.A., Damerval, C., Landry, J., Lea, P.J., Bellato, C.M., Meinhardt, L.W., Le Guilloux, M., Delhaye, S., Toro, A.A., Gaziola, S.A. Berdejo, B.D. (2003) Regulation of maize lysine metabolism and endosperm protein synthesis by opaque and floury mutations. Eur. J. Biochem. 270, 4898 4908.
4. Azevedo, R.A., Damerval, C., Lea, P.J., Landry, J., Bellato, C.M., Meinhardt, L.W., Le Guilloux, M., Delhaye, S., Toro, A.A., Gaziola, S.A., Varisi, V.A. Gratao, P.L. (2004a) Genetic control of lysine metabolism in maize endosperm mutants. Func. Plant Biol. 31, 339 348.
5. Azevedo, R.A., Lea, P.J., Damerval, C., Landry, J., Bellato, C.M., Meinhardt, L.W., Le Guilloux, M., Delhaye, S., Varisi, V.A., Gaziola, S.A., Gratao, P.L. Toro, A.A. (2004b) Regulation of lysine metabolism and endosperm protein synthesis by the opaque-5 and opaque-7 maize mutations. J. Agric. Food Chem. 52, 4865 4871.
6. Azevedo, R.A., Lancien, M. Lea, P.J. (2006) The aspartic acid metabolic pathway, an exciting and essential pathway in plants. Amino Acids, 30, 143 162.
7. Brown, S.M. Santino, C.G. (1999) Enhanced Expression in Plants. St. Louis, MO : Monsanto Company.
8. Dellaporta, S. (1994) Plant DNA miniprep and microprep. In The Maize Handbook (Freeling, M. Walbot, V., eds), pp. 522 525. New York : Springer-Verlag.
9. Falco, S.C., Keeler, S.J. Rice, J.A. (1998) Chimeric Genes and Methods for Increasing the Lysine and Threonine Content of the Seeds of Plants. Wilmington, DE : E. I. du Pont de Nemours and Company.
10. Hamilton, A., Voinnet, O., Chappell, L. Baulcombe, D. (2002) Two classes of short interfering RNA in RNA silencing. EMBO J. 21, 4671 4679.
11. Hartings, H., Lazzaroni, N., Marsan, P.A., Aragay, A., Thompson, R., Salamini, F., Di Fonzo, N., Palau, J. Motto, M. (1990) The b-32 protein from maize endosperm: characterization of genomic sequences encoding two alternative central domains. Plant Mol. Biol. 14, 1031 1040.
12. Heck, G.R., Armstrong, C.L., Astwood, J.D., Behr, C.F., Bookout, J.T., Brown, S.M., Cavato, T.A., DeBoer, D.L., Deng, M.Y., George, C., Hillyard, J.R., Hironaka, C.M., Howe, A.R., Jakse, E.H., Ledesma, B.E., Lee, T.C., Lirette, R.P., Mangano, M.L., Mutz, J.N., Qi, Y., Rodriguez, R.E., Sidhu, S.R., Silvanovich, A., Stoecker, M.A., Yingling, R.A. You, J. (2005) Development and characterization of a CP4 EPSPS-based, glyphosate-tolerant corn event. Crop Sci. 45, 329 339.
13. Houmard, N.M., Mainville, J.L., Bonin, C.P., Huang, S., Luethy, M.H. Malvar, T.M. (2007) High-lysine corn generated by endosperm-specific suppression of lysine catabolism using RNAi. Plant Biotechnol. J. 5, 605 614.
14. Huang, S., Adams, W.R., Zhou, Q., Malloy, K.P., Voyles, D.A., Anthony, J., Kriz, A.L. Luethy, M.H. (2004a) Improving nutritional quality of maize proteins by expressing sense and antisense zein genes. J. Agric. Food Chem. 52, 1958 1964.
15. Huang, S., Frizzi, A., Florida, C.A., Kruger, D.E. Luethy, M.H. (2006) High lysine and high tryptophan transgenic maize resulting from the reduction of both 19- and 22-kD alpha-zeins. Plant Mol. Biol. 61, 525 535.
16. Huang, S., Gilbertson, L.A., Adams, T.H., Malloy, K.P., Reisenbigler, E.K., Birr, D.H., Snyder, M.W., Zhang, Q. Luethy, M.H. (2004b) Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors. Transgenic. Res. 13, 451 461.
17. Huang, S., Kruger, D.E., Frizzi, A., D'Ordine, R.L., Florida, C.A., Adams, W.R., Brown, W.E. Luethy, M.H. (2005) High-lysine corn produced by the combination of enhanced lysine biosynthesis and reduced zein accumulation. Plant Biotechnol. J. 3, 555 569.
18. Johnson, L.A., Hardy, C.L., Baumel, C.P., Yu, T.H. Sell, J.L. (2001) Identifying valuable corn quality traits for livestock feed. Cereal Foods World, 46, 472 481.
19. Kay, R., Chan, A., Daly, M. Mcpherson, J. (1987) Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science, 236, 1299 1302.
20. Kemper, E.L., Neto, G.C., Papes, F., Moraes, K.C., Leite, A. Arruda, P. (1999) The role of opaque2 in the control of lysine-degrading activities in developing maize endosperm. Plant Cell, 11, 1981 1994.
21. Klahre, U., Crete, P., Leuenberger, S.A., Iglesias, V.A. Meins, F. Jr. (2002) High molecular weight RNAs and small interfering RNAs induce systemic posttranscriptional gene silencing in plants. Proc. Natl. Acad. Sci. USA, 99, 11 981 11 986.
22. Kriz, A.L., Luethy, M.H. Voyles, D.A. (1999) Methods and Compositions for Expression of Transgenes in Plants. Dekalb, IL : Dekalb Genetics Corporation.
23. Landry, J., Delhaye, S. Damerval, C. (2004) Protein distribution pattern in floury and vitreous endosperm of maize grain. Cereal Chem. 81, 153 158.
24. Lin, S.L., Chang, D., Wu, D.Y. Ying, S.Y. (2003) A novel RNA splicing-mediated gene silencing mechanism potential for genome evolution. Biochem. Biophys. Res. Commun. 310, 754 760.
25. Mazur, B., Krebbers, E. Tingey, S. (1999) Gene discovery and product development for grain quality traits. Science, 285, 372 375.
26. McElwain, E.F. Spiker, S. (1989) A wheat cDNA clone which is homologous to the 17 kd heat-shock protein gene family of soybean. Nucl. Acids Res. 17, 1764.
27. Mertz, E.T., Bates, L.S. Nelson, O.E. (1964) Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science, 145, 279 280.
28. Mlotshwa, S., Voinnet, O., Mette, M.F., Matzke, M., Vaucheret, H., Ding, S.W., Pruss, G. Vance, V.B. (2002) RNA silencing and the mobile silencing signal. Plant Cell, 14, S289 S301.
29. Nelson, O.E., Mertz, E.T. Bates, L.S. (1965) Second mutant gene affecting the amino acid pattern of maize endosperm proteins. Science, 150, 1469 1470.
30. Rochester, D.E., Winer, J.A. Shah, D.M. (1986) The structure and expression of maize genes encoding the major heat shock protein, hsp70. EMBO J. 5, 451 458.
31. Samakoglu, S., Lisowski, L., Budak-Alpdogan, T., Usachenko, Y., Acuto, S., Di Marzo, R., Maggio, A., Zhu, P., Tisdale, J.F., Riviere, I. Sadelain, M. (2006) A genetic strategy to treat sickle cell anemia by coregulating globin transgene expression and RNA interference. Nat. Biotechnol. 24, 89 94.
32. Segal, G., Song, R. Messing, J. (2003) A new opaque variant of maize by a single dominant RNA-interference-inducing transgene. Genetics, 165, 387 397.
33. Sheen, J. (1993) Protein phosphatase activity is required for light-inducible gene expression in maize. EMBO J. 12, 3497 3505.
34. Stepansky, A., Less, H., Angelovici, R., Aharon, R., Zhu, X. Galili, G. (2006) Lysine catabolism, an effective versatile regulator of lysine level in plants. Amino Acids, 30, 121 125.
35. Sunkar, R., Girke, T., Jain, P.K. Zhu, J.K. (2005) Cloning and characterization of MicroRNAs from rice. Plant Cell, 17, 1397 1411.
36. Vaistij, F.E., Jones, L. Baulcombe, D.C. (2002) Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase. Plant Cell, 14, 857 867.
37. Xie, Z.X., Johansen, L.K., Gustafson, A.M., Kasschau, K.D., Lellis, A.D., Zilberman, D., Jacobsen, S.E. Carrington, J.C. (2004) Genetic and functional diversification of small RNA pathways in plants. Plos Biol. 2, 642 652.
38. Ying, S.Y. Lin, S.L. (2004) Intron-derived microRNAs - fine tuning of gene functions. Gene, 342, 25 28.
39. Yoshikawa, M., Peragine, A., Park, M.Y. Poethig, R.S. (2005) A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis. Genes Dev, 19, 2164 2175.
40. Zhou, H.X., Xia, X.G. Xu, Z.S. (2005) An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi. Nucl. Acids Res. 33, e62.
41. Zhu, X. Galili, G. (2003) Increased lysine synthesis coupled with a knockout of its catabolism synergistically boosts lysine content and also transregulates the metabolism of other amino acids in Arabidopsis seeds. Plant Cell, 15, 845 853.
42. Zhu, X. Galili, G. (2004) Lysine metabolism is concurrently regulated by synthesis and catabolism in both reproductive and vegetative tissues. Plant Physiol. 135, 129 136.
43. Zhu, X., Tang, G., Granier, F., Bouchez, D. Galili, G. (2001) A T-DNA insertion knockout of the bifunctional lysine-ketoglutarate reductase/ saccharopine dehydrogenase gene elevates lysine levels in Arabidopsis seeds. Plant Physiol. 126, 1539 1545.