Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase

BMC Plant Biology

Volumn 6, Issue , 2006, Pages

  Diretto, Gianfranco ^a   Tavazza, Raffaela ^a   Welsch, Ralf ^b   Pizzichini, Daniele ^a   Mourgues, Fabienne ^a   Papacchioli, Velia ^a   Beyer, Peter ^b   Giuliano, Giovanni ^a  

^a ENEA C R CASACCIA   (Italy)

^b UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AGROBACTERIUM; RHIZOBIUM; SOLANUM TUBEROSUM;

BETA CAROTENE; CAROTENOID; ISOMERASE; LYCOPENE CYCLASE-ISOMERASE; XANTHOPHYLL;

BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC ENGINEERING; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; PLANT LEAF; PLANT TUBER; PLASMID; POTATO; PROCEDURES; RHIZOBIUM; TRANSGENIC PLANT;

BETA CAROTENE; CAROTENOIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; GENETIC ENGINEERING; INTRAMOLECULAR LYASES; LUTEIN; PLANT LEAVES; PLANT TUBERS; PLANTS, GENETICALLY MODIFIED; PLASMIDS; RHIZOBIUM; SOLANUM TUBEROSUM;

EID: 33748956342     PISSN: 14712229     EISSN: 14712229     Source Type: Journal    
DOI: 10.1186/1471-2229-6-13     Document Type: Article

References (38)

1. [http://www.cipotato.org/market/potatofacts/growprod.htm].
2. [http://www.who.int/nutrition/topics/vad/en/].
3. Giuliano G, Aquilani R, Dharmapuri S: Metabolic engineering of plant carotenoids. Trends Plant Sci 2000, 5(10):406-409.
4. Fraser PD, Bramley PM: The biosynthesis and nutritional uses of carotenoids. Prog Lipid Res 2004, 43(3):228-265.
5. Burkhardt PK, Beyer P, Wunn J, Kloti A, Armstrong GA, Schledz M, von Lintig J, Potrykus I: Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. Plant J 1997, 11(5):1071-1078.
6. Shewmaker CK, Sheehy JA, Daley M, Colburn S, Ke DY: Seed-specific overexpression of phytoene synthase: increase in carotenoids and other metabolic effects. Plant J 1999, 20(4):401-412.
7. Fraser PD, Romer S, Shipton CA, Mills PB, Kiano JW, Misawa N, Drake RG, Schuch W, Bramley PM: Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner. Proc Natl Acad Sci U S A 2002, 99(2):1092-1097.
8. Ducreux LJ, Morris WL, Hedley PE, Shepherd T, Davies HV, Millam S, Taylor MA: Metabolic engineering of high carotenoid potato tubers containing enhanced levels of beta-carotene and lutein. J Exp Bot 2005, 56(409):81-89.
9. Rosati C, Aquilani R, Dharmapuri S, Pallara P, Marusic C, Tavazza R, Bouvier F, Camara B, Giuliano G: Metabolic engineering of beta-carotene and lycopene content in tomato fruit. Plant J 2000, 24(3):413-420.
10. Ronen G, Carmel-Goren L, Zamir D, Hirschberg J: An alternative pathway to beta -carotene formation in plant chromoplasts discovered by map-based cloning of beta and old-gold color mutations in tomato. Proc Natl Acad Sci U S A 2000, 97(20):11102-11107.
11. Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, Beyer P, Potrykus I: Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 2000, 287(5451):303-305.
12. Dharmapuri S, Rosati C, Pallara P, Aquilani R, Bouvier F, Camara B, Giuliano G: Metabolic engineering of xanthophyll content in tomato fruits. FEBS Lett 2002, 519(1-3):30-34.
13. Mann V, Harker M, Pecker I, Hirschberg J: Metabolic engineering of astaxanthin production in tobacco flowers. Nat Biotechnol 2000, 18(8):888-892.
14. Ralley L, Enfissi EM, Misawa N, Schuch W, Bramley PM, Fraser PD: Metabolic engineering of ketocarotenoid formation in higher plants. Plant J 2004, 39(4):477-486.
15. Morris WL, Ducreux LJ, Fraser PD, Millam S, Taylor MA: Engineering ketocarotenoid biosynthesis in potato tubers. Metab Eng 2006, 8(3):253-263.
16. Romer S, Lubeck J, Kauder F, Steiger S, Adomat C, Sandmann G: Genetic engineering of a zeaxanthin-rich potato by antisense inactivation and co-suppression of carotenoid epoxidation. Metab Eng 2002, 4(4):263-272.
17. Pogson B, McDonald KA, Truong M, Britton G, DellaPenna D: Arabidopsis carotenoid mutants demonstrate that lutein is not essential for photosynthesis in higher plants. Plant Cell 1996, 8(9):1627-1639.
18. Dall'Osto L, Lico C, Alric J, Giuliano G, Havaux M, Bassi R: Lutein is needed for efficient chlorophyll triplet quenching in the major LHCII antenna complex of higher plants and effective photoprotection in vivo under strong light. in press.
19. Davuluri GR, van Tuinen A, Fraser PD, Manfredonia A, Newman R, Burgess D, Brummell DA, King SR, Palys J, Uhlig J, Bramley PM, Pennings HM, Bowler C: Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes. Nat Biotechnol 2005, 23(7):890-895.
20. Rocha-Sosa M, Sonnewald UWF, Stratmann M, Schell J, Willmitzer L: Both developmental and metabolic signals activate the promoter of a class I patatin gene. EMBO J 1989, 8(1):23-29.
21. Jefferson AR, Kavanagh TA, Bevan MW: GUS fusion: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 1987, 6(13):3901-3907.
22. Tavazza R, Tavazza M, Ordas RJ, Ancora G, Benvenuto E: Genetic transformation of potato (Solanum tuberosum); an efficient method to obtain transgenic plants. Plant Science 1988, 59:175-181.
23. Giuliano G, Bartley GE, Scolnik PA: Regulation of carotenoid biosynthesis during tomato development. Plant Cell 1993, 5(4):379-387.
24. Corona V, Aracri B, Kosturkova G, Bartley GE, Pitto L, Giorgetti L, Scolnik PA, Giuliano G: Regulation of a carotenoid biosynthesis gene promoter during plant development. Plant J 1996, 9(4):505-512.
25. Lange BM: Integrative analysis of metabolic networks: from peaks to flux models? Curr Opin Plant Biol 2006, 9:1-7.
26. Jorgensen RA, Cluster PD, English J, Que QD, Napoli CA: Chalcone synthase cosuppression phenotypes in petunia flowers: Comparison of sense vs antisense constructs and single-copy vs complex T-DNA sequences. Plant Mol Biol 1996, 31(5):957-973.
27. Waterhouse PM, Helliwell CA: Exploring plant genomes by RNA-induced gene silencing. Nat Rev Genet 2003, 4(1):29-38.
28. Tian L, Musetti V, Kim J, Magallanes-Lundback M, DellaPenna D: The Arabidopsis LUT1 locus encodes a member of the cytochrome p450 family that is required for carotenoid epsilonring hydroxylation activity. Proc Natl Acad Sci U S A 2004, 101(1):402-407.
29. Kim J, DellaPenna D: Defining the primary route for lutein synthesis in plants: the role of Arabidopsis carotenoid beta-ring hydroxylase CYP97A3. Proc Natl Acad Sci U S A 2006, 103(9):3474-3479.
30. Giuliano G, Al-Babili S, von Lintig J: Carotenoid oxygenases: cleave it or leave it. Trends Plant Sci 2003, 8(4):145-149.
31. Hirschberg J: Carotenoid biosynthesis in flowering plants. Curr Opin Plant Biol 2001, 4(3):210-218.
32. Bramley PM, Teulieres C, Blain I, Bird C, Schuch W: Biochemical characterization of transgenic tomato plants in which carotenoid synthesis has been inhibited through the expression of antisense RNA to pTOM5. Plant J 1992, 2:343-349.
33. Fray RG, Grierson D: Identification and genetic analysis of normal and mutant phytoene synthase genes of tomato by sequencing, complementation and co-suppression. Plant Mol Biol 1993, 22(4):589-602.
34. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning. A Laboratory Manual (Second Edition). Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 1989.
35. Carbone F, Pizzichini D, Giuliano G, Rosati C, Perrotta G: Comparative profiling of tomato fruits and leaves evidences a complex modulation of global transcript profiles. Plant Sci 2005, 169:165-175.
36. [http://www.tigr.org/].
37. Hoa TT, Al-Babili S, Schaub P, Potrykus I, Beyer P: Golden Indica and Japonica rice lines amenable to deregulation. Plant Physiol 2003, 133(1):161-169.
38. Schaub P, Al-Babili S, Drake R, Beyer P: Why is golden rice golden (yellow) instead of red? Plant Physiol 2005, 138(1):441-450.