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Volumn 53, Issue , 2018, Pages 191-200

Genetic engineering of host organisms for pharmaceutical synthesis

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CELL CULTURE; DNA SEQUENCES; GENE ENCODING; RECOMBINANT PROTEINS;

EID: 85042184957     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2018.02.001     Document Type: Review
Times cited : (20)

References (110)
  • 1
    • 84968903135 scopus 로고    scopus 로고
    • Coming of age: ten years of next-generation sequencing technologies
    • An excellent comprehensive review of sequencing technologies that describes and compares each approach.
    • Goodwin, S., McPherson, J.D., McCombie, W.R., Coming of age: ten years of next-generation sequencing technologies. Nat Rev Genet 17 (2016), 333–351 An excellent comprehensive review of sequencing technologies that describes and compares each approach.
    • (2016) Nat Rev Genet , vol.17 , pp. 333-351
    • Goodwin, S.1    McPherson, J.D.2    McCombie, W.R.3
  • 2
    • 84899696363 scopus 로고    scopus 로고
    • Large-scale de novo DNA synthesis: technologies and applications
    • Kosuri, S., Church, G.M., Large-scale de novo DNA synthesis: technologies and applications. Nat Methods 11 (2014), 499–507.
    • (2014) Nat Methods , vol.11 , pp. 499-507
    • Kosuri, S.1    Church, G.M.2
  • 3
    • 85014834845 scopus 로고    scopus 로고
    • Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond
    • This work describes a landmark in genome engineering — full yeast chromosome synthesis. This is part of the worldwide Yeast2.0 synthetic genome project. By organizing and condensing the yeast genome, unprecedented insight into gene function and genome biology is possible.
    • Mitchell, L.A., Wang, A., Stracquadanio, G., Kuang, Z., Wang, X., Yang, K., Richardson, S., Martin, J.A., Zhao, Y., Walker, R., et al. Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond. Science, 2017, 355 This work describes a landmark in genome engineering — full yeast chromosome synthesis. This is part of the worldwide Yeast2.0 synthetic genome project. By organizing and condensing the yeast genome, unprecedented insight into gene function and genome biology is possible.
    • (2017) Science , pp. 355
    • Mitchell, L.A.1    Wang, A.2    Stracquadanio, G.3    Kuang, Z.4    Wang, X.5    Yang, K.6    Richardson, S.7    Martin, J.A.8    Zhao, Y.9    Walker, R.10
  • 5
    • 84898778301 scopus 로고    scopus 로고
    • A guide to genome engineering with programmable nucleases
    • Kim, H., Kim, J.S., A guide to genome engineering with programmable nucleases. Nat Rev Genet 15 (2014), 321–334.
    • (2014) Nat Rev Genet , vol.15 , pp. 321-334
    • Kim, H.1    Kim, J.S.2
  • 7
    • 85011003404 scopus 로고    scopus 로고
    • High efficiency hydrodynamic bacterial electrotransformation
    • This work establishes electroporation in microfluidic channels, a new approach that could permit transformation of new microorganisms. The results suggest that the types of electric fields in this design allow increases in efficiency and scale over cuvettes.
    • Garcia, P.A., Ge, Z., Kelley, L.E., Holcomb, S.J., Buie, C.R., High efficiency hydrodynamic bacterial electrotransformation. Lab Chip 17 (2017), 490–500 This work establishes electroporation in microfluidic channels, a new approach that could permit transformation of new microorganisms. The results suggest that the types of electric fields in this design allow increases in efficiency and scale over cuvettes.
    • (2017) Lab Chip , vol.17 , pp. 490-500
    • Garcia, P.A.1    Ge, Z.2    Kelley, L.E.3    Holcomb, S.J.4    Buie, C.R.5
  • 8
    • 84959019830 scopus 로고    scopus 로고
    • Microfluidic screening of electric fields for electroporation
    • Garcia, P.A., Ge, Z., Moran, J.L., Buie, C.R., Microfluidic screening of electric fields for electroporation. Sci Rep, 6, 2016, 21238.
    • (2016) Sci Rep , vol.6 , pp. 21238
    • Garcia, P.A.1    Ge, Z.2    Moran, J.L.3    Buie, C.R.4
  • 9
    • 84920896587 scopus 로고    scopus 로고
    • Next-generation genome-scale models for metabolic engineering
    • This review highlights progress and perspectives on Constraint Based Reconstruction and Analysis (COBRA). The authors describe recent achievement using this metabolic modeling approach and how additional predictions with COBRA models could advance metabolic engineering in the future.
    • King, Z.A., Lloyd, C.J., Feist, A.M., Palsson, B.O., Next-generation genome-scale models for metabolic engineering. Curr Opin Biotechnol 35 (2015), 23–29 This review highlights progress and perspectives on Constraint Based Reconstruction and Analysis (COBRA). The authors describe recent achievement using this metabolic modeling approach and how additional predictions with COBRA models could advance metabolic engineering in the future.
    • (2015) Curr Opin Biotechnol , vol.35 , pp. 23-29
    • King, Z.A.1    Lloyd, C.J.2    Feist, A.M.3    Palsson, B.O.4
  • 12
    • 85038640210 scopus 로고    scopus 로고
    • Leaf LIMS: a flexible laboratory information management system with a synthetic biology focus
    • The authors present a freely available laboratory information management system specifically designed for synthetic biology. This software is a great resource for genetic engineering labs, where organization of DNA parts, for example, can be a challenge.
    • Craig, T., Holland, R., D'Amore, R., Johnson, J.R., McCue, H.V., West, A., Zulkower, V., Tekotte, H., Cai, Y., Swan, D., et al. Leaf LIMS: a flexible laboratory information management system with a synthetic biology focus. ACS Synth Biol 6 (2017), 2273–2280 The authors present a freely available laboratory information management system specifically designed for synthetic biology. This software is a great resource for genetic engineering labs, where organization of DNA parts, for example, can be a challenge.
    • (2017) ACS Synth Biol , vol.6 , pp. 2273-2280
    • Craig, T.1    Holland, R.2    D'Amore, R.3    Johnson, J.R.4    McCue, H.V.5    West, A.6    Zulkower, V.7    Tekotte, H.8    Cai, Y.9    Swan, D.10
  • 13
    • 84963568291 scopus 로고    scopus 로고
    • Genetic circuit design automation
    • The authors report 45 perfectly functioning circuits out of 60 designs without expert tuning. To accomplish this, the authors develop Cello, software that can output functional circuits composed of a set of well-characterized logic gates from user-inputted logic. This represents a landmark achievement for synthetic biology.
    • Nielsen, A.A., Der, B.S., Shin, J., Vaidyanathan, P., Paralanov, V., Strychalski, E.A., Ross, D., Densmore, D., Voigt, C.A., Genetic circuit design automation. Science, 352, 2016, aac7341 The authors report 45 perfectly functioning circuits out of 60 designs without expert tuning. To accomplish this, the authors develop Cello, software that can output functional circuits composed of a set of well-characterized logic gates from user-inputted logic. This represents a landmark achievement for synthetic biology.
    • (2016) Science , vol.352 , pp. aac7341
    • Nielsen, A.A.1    Der, B.S.2    Shin, J.3    Vaidyanathan, P.4    Paralanov, V.5    Strychalski, E.A.6    Ross, D.7    Densmore, D.8    Voigt, C.A.9
  • 14
    • 85020737000 scopus 로고    scopus 로고
    • Automated multiplex genome-scale engineering in yeast
    • An automated platform for multiplexed genome-scale engineering in S. cerevisiae was constructed. Genetic parts encoding overexpression and knockdown mutations were iteratively integrated by CRISPR-Cas in an automated workflow. This will greatly accelerate genome-scale engineering for a desired phenotype in S. cerevisiae, and could be adapted to other organisms.
    • Si, T., Chao, R., Min, Y., Wu, Y., Ren, W., Zhao, H., Automated multiplex genome-scale engineering in yeast. Nat Commun, 8, 2017, 15187 An automated platform for multiplexed genome-scale engineering in S. cerevisiae was constructed. Genetic parts encoding overexpression and knockdown mutations were iteratively integrated by CRISPR-Cas in an automated workflow. This will greatly accelerate genome-scale engineering for a desired phenotype in S. cerevisiae, and could be adapted to other organisms.
    • (2017) Nat Commun , vol.8 , pp. 15187
    • Si, T.1    Chao, R.2    Min, Y.3    Wu, Y.4    Ren, W.5    Zhao, H.6
  • 16
    • 84906217475 scopus 로고    scopus 로고
    • Comprehensive mutational scanning of a kinase in vivo reveals substrate-dependent fitness landscapes
    • Melnikov, A., Rogov, P., Wang, L., Gnirke, A., Mikkelsen, T.S., Comprehensive mutational scanning of a kinase in vivo reveals substrate-dependent fitness landscapes. Nucleic Acids Res, 42, 2014, e112.
    • (2014) Nucleic Acids Res , vol.42 , pp. e112
    • Melnikov, A.1    Rogov, P.2    Wang, L.3    Gnirke, A.4    Mikkelsen, T.S.5
  • 17
    • 84958146942 scopus 로고    scopus 로고
    • Algorithmic co-optimization of genetic constructs and growth conditions: application to 6-ACA, a potential nylon-6 precursor
    • Zhou, H., Vonk, B., Roubos, J.A., Bovenberg, R.A., Voigt, C.A., Algorithmic co-optimization of genetic constructs and growth conditions: application to 6-ACA, a potential nylon-6 precursor. Nucleic Acids Res 43 (2015), 10560–10570.
    • (2015) Nucleic Acids Res , vol.43 , pp. 10560-10570
    • Zhou, H.1    Vonk, B.2    Roubos, J.A.3    Bovenberg, R.A.4    Voigt, C.A.5
  • 18
    • 85012838041 scopus 로고    scopus 로고
    • Improving metabolic pathway efficiency by statistical model-based multivariate regulatory metabolic engineering
    • A promoter library was developed to allow different expression levels of the violacein pathway in E. coli. A combinatorial set of different expression variants was designed with a statistical model, specifically design-of-experiments (DoE). These models were used to correlate gene expression levels with violacein titer. Extracting useful design information from rationally reduced combinatorial libraries promises to significantly decrease the experimental effort needed to iterate towards high producing phenotypes.
    • Xu, P., Rizzoni, E.A., Sul, S.Y., Stephanopoulos, G., Improving metabolic pathway efficiency by statistical model-based multivariate regulatory metabolic engineering. ACS Synth Biol 6 (2017), 148–158 A promoter library was developed to allow different expression levels of the violacein pathway in E. coli. A combinatorial set of different expression variants was designed with a statistical model, specifically design-of-experiments (DoE). These models were used to correlate gene expression levels with violacein titer. Extracting useful design information from rationally reduced combinatorial libraries promises to significantly decrease the experimental effort needed to iterate towards high producing phenotypes.
    • (2017) ACS Synth Biol , vol.6 , pp. 148-158
    • Xu, P.1    Rizzoni, E.A.2    Sul, S.Y.3    Stephanopoulos, G.4
  • 20
    • 84856389498 scopus 로고    scopus 로고
    • CHO cells in biotechnology for production of recombinant proteins: current state and further potential
    • Kim, J.Y., Kim, Y.G., Lee, G.M., CHO cells in biotechnology for production of recombinant proteins: current state and further potential. Appl Microbiol Biotechnol 93 (2012), 917–930.
    • (2012) Appl Microbiol Biotechnol , vol.93 , pp. 917-930
    • Kim, J.Y.1    Kim, Y.G.2    Lee, G.M.3
  • 22
    • 84865070369 scopus 로고    scopus 로고
    • A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
    • Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A., Charpentier E: A., A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337 (2012), 816–821.
    • (2012) Science , vol.337 , pp. 816-821
    • Jinek, M.1    Chylinski, K.2    Fonfara, I.3    Hauer, M.4    Doudna, J.A.5    Charpentier E:, A.6
  • 23
    • 84952639685 scopus 로고    scopus 로고
    • Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation
    • An informative review of alternative uses of the Cas9 nuclease, including strategies for inducible gene regulation, genome-wide screens, and cell fate engineering.
    • Dominguez, A.A., Lim, W.A., Qi, L.S., Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation. Nat Rev Mol Cell Biol 17 (2016), 5–15 An informative review of alternative uses of the Cas9 nuclease, including strategies for inducible gene regulation, genome-wide screens, and cell fate engineering.
    • (2016) Nat Rev Mol Cell Biol , vol.17 , pp. 5-15
    • Dominguez, A.A.1    Lim, W.A.2    Qi, L.S.3
  • 31
    • 85027284460 scopus 로고    scopus 로고
    • The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro
    • In this work, the recently discovered Cpf1 nuclease was adapted as a flexible cloning tool. DNA cleavage with the Cpf1 nuclease domain creates sticky ends, which the authors show can facilitate in vitro molecular cloning reactions. Like Gibson assembly, Cpf1 cloning is scarless, but has the added advantage of being much more efficient with high GC regions.
    • Lei, C., Li, S.Y., Liu, J.K., Zheng, X., Zhao, G.P., Wang, J., The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro. Nucleic Acids Res, 45, 2017, e74 In this work, the recently discovered Cpf1 nuclease was adapted as a flexible cloning tool. DNA cleavage with the Cpf1 nuclease domain creates sticky ends, which the authors show can facilitate in vitro molecular cloning reactions. Like Gibson assembly, Cpf1 cloning is scarless, but has the added advantage of being much more efficient with high GC regions.
    • (2017) Nucleic Acids Res , vol.45 , pp. e74
    • Lei, C.1    Li, S.Y.2    Liu, J.K.3    Zheng, X.4    Zhao, G.P.5    Wang, J.6
  • 32
    • 85006401289 scopus 로고    scopus 로고
    • C-Brick: a new standard for assembly of biological parts using Cpf1
    • Li, S.Y., Zhao, G.P., Wang, J., C-Brick: a new standard for assembly of biological parts using Cpf1. ACS Synth Biol 5 (2016), 1383–1388.
    • (2016) ACS Synth Biol , vol.5 , pp. 1383-1388
    • Li, S.Y.1    Zhao, G.P.2    Wang, J.3
  • 34
    • 85006511857 scopus 로고    scopus 로고
    • PAM-dependent target DNA recognition and cleavage by C2c1 CRISPR-Cas endonuclease
    • e1812
    • Yang, H., Gao, P., Rajashankar, K.R., Patel, D.J., PAM-dependent target DNA recognition and cleavage by C2c1 CRISPR-Cas endonuclease. Cell 167 (2016), 1814–1828 e1812.
    • (2016) Cell , vol.167 , pp. 1814-1828
    • Yang, H.1    Gao, P.2    Rajashankar, K.R.3    Patel, D.J.4
  • 40
    • 84923925063 scopus 로고    scopus 로고
    • Synthetic biology advances for pharmaceutical production
    • Breitling, R., Takano, E., Synthetic biology advances for pharmaceutical production. Curr Opin Biotechnol 35 (2015), 46–51.
    • (2015) Curr Opin Biotechnol , vol.35 , pp. 46-51
    • Breitling, R.1    Takano, E.2
  • 41
    • 85020672990 scopus 로고    scopus 로고
    • A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae
    • Ren, H., Hu, P., Zhao, H., A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae. Biotechnol Bioeng 114 (2017), 1847–1854.
    • (2017) Biotechnol Bioeng , vol.114 , pp. 1847-1854
    • Ren, H.1    Hu, P.2    Zhao, H.3
  • 43
    • 85039041158 scopus 로고    scopus 로고
    • Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
    • This paper applies the extensive toolbox in S. cerevisiae to produce penicillin for the first time in yeast. This achievement required an integrated workflow using characterized synthetic biology parts, modular Golden Gate assembly, CRISPR-assisted genome integration, pathway engineering, nanopore sequencing, and bioinformatics software.
    • Awan, A.R., Blount, B.A., Bell, D.J., Shaw, W.M., Ho, J.C.H., McKiernan, R.M., Ellis, T., Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast. Nat Commun, 2017, 8 This paper applies the extensive toolbox in S. cerevisiae to produce penicillin for the first time in yeast. This achievement required an integrated workflow using characterized synthetic biology parts, modular Golden Gate assembly, CRISPR-assisted genome integration, pathway engineering, nanopore sequencing, and bioinformatics software.
    • (2017) Nat Commun , pp. 8
    • Awan, A.R.1    Blount, B.A.2    Bell, D.J.3    Shaw, W.M.4    Ho, J.C.H.5    McKiernan, R.M.6    Ellis, T.7
  • 45
    • 84894461463 scopus 로고    scopus 로고
    • Bioprospecting in the genomic age
    • Hicks, M.A., Prather, K.L., Bioprospecting in the genomic age. Adv Appl Microbiol 87 (2014), 111–146.
    • (2014) Adv Appl Microbiol , vol.87 , pp. 111-146
    • Hicks, M.A.1    Prather, K.L.2
  • 49
    • 84890395226 scopus 로고    scopus 로고
    • Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay
    • Lee, M.E., Aswani, A., Han, A.S., Tomlin, C.J., Dueber, J.E., Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay. Nucleic Acids Res 41 (2013), 10668–10678.
    • (2013) Nucleic Acids Res , vol.41 , pp. 10668-10678
    • Lee, M.E.1    Aswani, A.2    Han, A.S.3    Tomlin, C.J.4    Dueber, J.E.5
  • 50
    • 84890178034 scopus 로고    scopus 로고
    • Advances in genetic circuit design: novel biochemistries, deep part mining, and precision gene expression
    • Nielsen, A.A., Segall-Shapiro, T.H., Voigt, C.A., Advances in genetic circuit design: novel biochemistries, deep part mining, and precision gene expression. Curr Opin Chem Biol 17 (2013), 878–892.
    • (2013) Curr Opin Chem Biol , vol.17 , pp. 878-892
    • Nielsen, A.A.1    Segall-Shapiro, T.H.2    Voigt, C.A.3
  • 51
    • 85013374260 scopus 로고    scopus 로고
    • Iterative optimization of xylose catabolism in Saccharomyces cerevisiae using combinatorial expression tuning
    • Latimer, L.N., Dueber, J.E., Iterative optimization of xylose catabolism in Saccharomyces cerevisiae using combinatorial expression tuning. Biotechnol Bioeng 114 (2017), 1301–1309.
    • (2017) Biotechnol Bioeng , vol.114 , pp. 1301-1309
    • Latimer, L.N.1    Dueber, J.E.2
  • 52
    • 84989852376 scopus 로고    scopus 로고
    • Rewriting yeast central carbon metabolism for industrial isoprenoid production
    • Key bottlenecks in cytosolic acetyl-CoA production in S. cerevisiae were overcome with alternative central carbon metabolism pathways. This, coupled with non-obvious mutations, allowed massive increases in production of the isoprenoid farnesene. This work is an excellent example of the development effort necessary to create a chassis strain for a particular chemical group.
    • Meadows, A.L., Hawkins, K.M., Tsegaye, Y., Antipov, E., Kim, Y., Raetz, L., Dahl, R.H., Tai, A., Mahatdejkul-Meadows, T., Xu, L., et al. Rewriting yeast central carbon metabolism for industrial isoprenoid production. Nature 537 (2016), 694–697 Key bottlenecks in cytosolic acetyl-CoA production in S. cerevisiae were overcome with alternative central carbon metabolism pathways. This, coupled with non-obvious mutations, allowed massive increases in production of the isoprenoid farnesene. This work is an excellent example of the development effort necessary to create a chassis strain for a particular chemical group.
    • (2016) Nature , vol.537 , pp. 694-697
    • Meadows, A.L.1    Hawkins, K.M.2    Tsegaye, Y.3    Antipov, E.4    Kim, Y.5    Raetz, L.6    Dahl, R.H.7    Tai, A.8    Mahatdejkul-Meadows, T.9    Xu, L.10
  • 53
    • 77955459156 scopus 로고    scopus 로고
    • Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides
    • Warner, J.R., Reeder, P.J., Karimpour-Fard, A., Woodruff, L.B., Gill, R.T., Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides. Nat Biotechnol 28 (2010), 856–862.
    • (2010) Nat Biotechnol , vol.28 , pp. 856-862
    • Warner, J.R.1    Reeder, P.J.2    Karimpour-Fard, A.3    Woodruff, L.B.4    Gill, R.T.5
  • 54
    • 85034039076 scopus 로고    scopus 로고
    • Production of chemicals using dynamic control of metabolic fluxes
    • Xu, P., Production of chemicals using dynamic control of metabolic fluxes. Curr Opin Biotechnol 53 (2017), 12–19.
    • (2017) Curr Opin Biotechnol , vol.53 , pp. 12-19
    • Xu, P.1
  • 55
    • 84920747663 scopus 로고    scopus 로고
    • Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites
    • Brockman, I.M., Prather, K.L., Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites. Metab Eng 28 (2015), 104–113.
    • (2015) Metab Eng , vol.28 , pp. 104-113
    • Brockman, I.M.1    Prather, K.L.2
  • 56
    • 84984666893 scopus 로고    scopus 로고
    • Vibrio natriegens as a fast-growing host for molecular biology
    • Genetic tools were developed for the extremely fast-growing V. natriegens, which the authors argue to speed molecular biology workflows dependent on E. coli.
    • Weinstock, M.T., Hesek, E.D., Wilson, C.M., Gibson, D.G., Vibrio natriegens as a fast-growing host for molecular biology. Nat Methods 13 (2016), 849–851 Genetic tools were developed for the extremely fast-growing V. natriegens, which the authors argue to speed molecular biology workflows dependent on E. coli.
    • (2016) Nat Methods , vol.13 , pp. 849-851
    • Weinstock, M.T.1    Hesek, E.D.2    Wilson, C.M.3    Gibson, D.G.4
  • 57
    • 85029539622 scopus 로고    scopus 로고
    • Multiplex genome editing by natural transformation (MuGENT) for synthetic biology in Vibrio natriegens
    • Dalia, T.N., Hayes, C.A., Stolyar, S., Marx, C.J., McKinlay, J.B., Dalia, A.B., Multiplex genome editing by natural transformation (MuGENT) for synthetic biology in Vibrio natriegens. ACS Synth Biol 6 (2017), 1650–1655.
    • (2017) ACS Synth Biol , vol.6 , pp. 1650-1655
    • Dalia, T.N.1    Hayes, C.A.2    Stolyar, S.3    Marx, C.J.4    McKinlay, J.B.5    Dalia, A.B.6
  • 58
    • 85031815634 scopus 로고    scopus 로고
    • Metabolism of the fast-growing bacterium Vibrio natriegens elucidated by 13C metabolic flux analysis
    • Long, C.P., Gonzalez, J.E., Cipolla, R.M., Antoniewicz, M.R., Metabolism of the fast-growing bacterium Vibrio natriegens elucidated by 13C metabolic flux analysis. Metab Eng 44 (2017), 191–197.
    • (2017) Metab Eng , vol.44 , pp. 191-197
    • Long, C.P.1    Gonzalez, J.E.2    Cipolla, R.M.3    Antoniewicz, M.R.4
  • 59
    • 85018404578 scopus 로고    scopus 로고
    • Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous beta-carotene production
    • Gao, S., Tong, Y., Zhu, L., Ge, M., Zhang, Y., Chen, D., Jiang, Y., Yang, S., Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous beta-carotene production. Metab Eng 41 (2017), 192–201.
    • (2017) Metab Eng , vol.41 , pp. 192-201
    • Gao, S.1    Tong, Y.2    Zhu, L.3    Ge, M.4    Zhang, Y.5    Chen, D.6    Jiang, Y.7    Yang, S.8
  • 61
    • 84961820300 scopus 로고    scopus 로고
    • Engineering promoter architecture in the oleaginous yeast Yarrowia lipolytica
    • Shabbir Hussain, M., Gambill, L., Smith, S., Blenner, M.A., Engineering promoter architecture in the oleaginous yeast Yarrowia lipolytica. ACS Synth Biol 5 (2016), 213–223.
    • (2016) ACS Synth Biol , vol.5 , pp. 213-223
    • Shabbir Hussain, M.1    Gambill, L.2    Smith, S.3    Blenner, M.A.4
  • 62
    • 85028771240 scopus 로고    scopus 로고
    • CRISPRi repression of nonhomologous end-joining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica
    • Schwartz, C., Frogue, K., Ramesh, A., Misa, J., Wheeldon, I., CRISPRi repression of nonhomologous end-joining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica. Biotechnol Bioeng 114 (2017), 2896–2906.
    • (2017) Biotechnol Bioeng , vol.114 , pp. 2896-2906
    • Schwartz, C.1    Frogue, K.2    Ramesh, A.3    Misa, J.4    Wheeldon, I.5
  • 63
    • 84966311094 scopus 로고    scopus 로고
    • Synthetic RNA polymerase III promoters facilitate high-efficiency CRISPR-Cas9-mediated genome editing in Yarrowia lipolytica
    • Schwartz, C.M., Hussain, M.S., Blenner, M., Wheeldon, I., Synthetic RNA polymerase III promoters facilitate high-efficiency CRISPR-Cas9-mediated genome editing in Yarrowia lipolytica. ACS Synth Biol 5 (2016), 356–359.
    • (2016) ACS Synth Biol , vol.5 , pp. 356-359
    • Schwartz, C.M.1    Hussain, M.S.2    Blenner, M.3    Wheeldon, I.4
  • 64
    • 85031777886 scopus 로고    scopus 로고
    • Heterologous production of myxobacterial alpha-pyrone antibiotics in Myxococcus xanthus
    • Sucipto, H., Pogorevc, D., Luxenburger, E., Wenzel, S.C., Muller, R., Heterologous production of myxobacterial alpha-pyrone antibiotics in Myxococcus xanthus. Metab Eng 44 (2017), 160–170.
    • (2017) Metab Eng , vol.44 , pp. 160-170
    • Sucipto, H.1    Pogorevc, D.2    Luxenburger, E.3    Wenzel, S.C.4    Muller, R.5
  • 65
    • 84959386848 scopus 로고    scopus 로고
    • Heterologous production of the marine myxobacterial antibiotic haliangicin and its unnatural analogues generated by engineering of the biochemical pathway
    • Sun, Y., Feng, Z., Tomura, T., Suzuki, A., Miyano, S., Tsuge, T., Mori, H., Suh, J.W., Iizuka, T., Fudou, R., et al. Heterologous production of the marine myxobacterial antibiotic haliangicin and its unnatural analogues generated by engineering of the biochemical pathway. Sci Rep, 6, 2016, 22091.
    • (2016) Sci Rep , vol.6 , pp. 22091
    • Sun, Y.1    Feng, Z.2    Tomura, T.3    Suzuki, A.4    Miyano, S.5    Tsuge, T.6    Mori, H.7    Suh, J.W.8    Iizuka, T.9    Fudou, R.10
  • 66
    • 84982107482 scopus 로고    scopus 로고
    • Development of a CRISPR-Cas9 tool kit for comprehensive engineering of Bacillus subtilis
    • Westbrook, A.W., Moo-Young, M., Chou, C.P., Development of a CRISPR-Cas9 tool kit for comprehensive engineering of Bacillus subtilis. Appl Environ Microbiol 82 (2016), 4876–4895.
    • (2016) Appl Environ Microbiol , vol.82 , pp. 4876-4895
    • Westbrook, A.W.1    Moo-Young, M.2    Chou, C.P.3
  • 67
    • 85029677509 scopus 로고    scopus 로고
    • Combinatorial promoter engineering of glucokinase and phosphoglucoisomerase for improved N-acetylglucosamine production in Bacillus subtilis
    • Ling, M., Liu, Y., Li, J., Shin, H.D., Chen, J., Du, G., Liu, L., Combinatorial promoter engineering of glucokinase and phosphoglucoisomerase for improved N-acetylglucosamine production in Bacillus subtilis. Bioresour Technol 245 (2017), 1093–1102.
    • (2017) Bioresour Technol , vol.245 , pp. 1093-1102
    • Ling, M.1    Liu, Y.2    Li, J.3    Shin, H.D.4    Chen, J.5    Du, G.6    Liu, L.7
  • 68
  • 70
    • 84937722803 scopus 로고    scopus 로고
    • Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters
    • Montiel, D., Kang, H.S., Chang, F.Y., Charlop-Powers, Z., Brady, S.F., Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters. Proc Natl Acad Sci U S A 112 (2015), 8953–8958.
    • (2015) Proc Natl Acad Sci U S A , vol.112 , pp. 8953-8958
    • Montiel, D.1    Kang, H.S.2    Chang, F.Y.3    Charlop-Powers, Z.4    Brady, S.F.5
  • 71
    • 84941777702 scopus 로고    scopus 로고
    • Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives
    • Dumont, J., Euwart, D., Mei, B., Estes, S., Kshirsagar, R., Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives. Crit Rev Biotechnol 36 (2016), 1110–1122.
    • (2016) Crit Rev Biotechnol , vol.36 , pp. 1110-1122
    • Dumont, J.1    Euwart, D.2    Mei, B.3    Estes, S.4    Kshirsagar, R.5
  • 72
    • 84944733586 scopus 로고    scopus 로고
    • Modifying an insect cell N-glycan processing pathway using CRISPR-Cas technology
    • Mabashi-Asazuma, H., Kuo, C.W., Khoo, K.H., Jarvis, D.L., Modifying an insect cell N-glycan processing pathway using CRISPR-Cas technology. ACS Chem Biol 10 (2015), 2199–2208.
    • (2015) ACS Chem Biol , vol.10 , pp. 2199-2208
    • Mabashi-Asazuma, H.1    Kuo, C.W.2    Khoo, K.H.3    Jarvis, D.L.4
  • 73
    • 84903814380 scopus 로고    scopus 로고
    • Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production
    • Ahmad, M., Hirz, M., Pichler, H., Schwab, H., Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Appl Microbiol Biotechnol 98 (2014), 5301–5317.
    • (2014) Appl Microbiol Biotechnol , vol.98 , pp. 5301-5317
    • Ahmad, M.1    Hirz, M.2    Pichler, H.3    Schwab, H.4
  • 74
    • 85030120086 scopus 로고    scopus 로고
    • Reexamining opportunities for therapeutic protein production in eukaryotic microorganisms
    • Bartlett, M.C., Wright, C., Kuo, A., Colant, N., Westoby, M., Love, J.C., Reexamining opportunities for therapeutic protein production in eukaryotic microorganisms. Biotechnol Bioeng 114 (2017), 2432–2444.
    • (2017) Biotechnol Bioeng , vol.114 , pp. 2432-2444
    • Bartlett, M.C.1    Wright, C.2    Kuo, A.3    Colant, N.4    Westoby, M.5    Love, J.C.6
  • 75
    • 85032582544 scopus 로고    scopus 로고
    • Promoter and terminator discovery and engineering
    • Deaner, M., Alper, H.S., Promoter and terminator discovery and engineering. Adv Biochem Eng Biotechnol 162 (2016), 21–44.
    • (2016) Adv Biochem Eng Biotechnol , vol.162 , pp. 21-44
    • Deaner, M.1    Alper, H.S.2
  • 76
    • 85020843976 scopus 로고    scopus 로고
    • A modular toolkit for generating Pichia pastoris secretion libraries
    • Obst, U., Lu, T.K., Sieber, V., A modular toolkit for generating Pichia pastoris secretion libraries. ACS Synth Biol 6 (2017), 1016–1025.
    • (2017) ACS Synth Biol , vol.6 , pp. 1016-1025
    • Obst, U.1    Lu, T.K.2    Sieber, V.3
  • 78
    • 84978765496 scopus 로고    scopus 로고
    • CRISPR/Cas9 based genome editing of Penicillium chrysogenum
    • The authors adapt many different Cas9 based editing approaches to function with P. chrysogenum. This work is a landmark achievement in engineering filamentous fungi, which produce a large number of important natural products.
    • Pohl, C., Kiel, J.A., Driessen, A.J., Bovenberg, R.A., Nygard, Y., CRISPR/Cas9 based genome editing of Penicillium chrysogenum. ACS Synth Biol 5 (2016), 754–764 The authors adapt many different Cas9 based editing approaches to function with P. chrysogenum. This work is a landmark achievement in engineering filamentous fungi, which produce a large number of important natural products.
    • (2016) ACS Synth Biol , vol.5 , pp. 754-764
    • Pohl, C.1    Kiel, J.A.2    Driessen, A.J.3    Bovenberg, R.A.4    Nygard, Y.5
  • 79
    • 85029366449 scopus 로고    scopus 로고
    • Toward establishing model organisms for marine protists: successful transfection protocols for Parabodo caudatus (Kinetoplastida: Excavata)
    • Gomaa, F., Garcia, P.A., Delaney, J., Girguis, P.R., Buie, C.R., Edgcomb, V.P., Toward establishing model organisms for marine protists: successful transfection protocols for Parabodo caudatus (Kinetoplastida: Excavata). Environ Microbiol 19 (2017), 3487–3499.
    • (2017) Environ Microbiol , vol.19 , pp. 3487-3499
    • Gomaa, F.1    Garcia, P.A.2    Delaney, J.3    Girguis, P.R.4    Buie, C.R.5    Edgcomb, V.P.6
  • 80
    • 85042197228 scopus 로고    scopus 로고
    • Transient silencing of DNA repair genes improves targeted gene integration in the filamentous fungus Trichoderma reesei
    • The authors transiently knocked down non-homologous end joining (NHEJ) proteins to increase the efficiency of homology-directed repair. Several previous efforts have knocked out these proteins to increase DNA integration efficiencies, but these earlier approaches could have unforeseen deletorious effects on cell growth and limit adaptation to industrial processes. Thus, this work represents an advanced tool for increasing integration efficiency.
    • Chum, P.Y., Schmidt, G., Saloheimo, M., Landowski, C.P., Transient silencing of DNA repair genes improves targeted gene integration in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol, 2017, 83 The authors transiently knocked down non-homologous end joining (NHEJ) proteins to increase the efficiency of homology-directed repair. Several previous efforts have knocked out these proteins to increase DNA integration efficiencies, but these earlier approaches could have unforeseen deletorious effects on cell growth and limit adaptation to industrial processes. Thus, this work represents an advanced tool for increasing integration efficiency.
    • (2017) Appl Environ Microbiol , pp. 83
    • Chum, P.Y.1    Schmidt, G.2    Saloheimo, M.3    Landowski, C.P.4
  • 82
    • 84978712445 scopus 로고    scopus 로고
    • Development of a synthetic oxytetracycline-inducible expression system for Streptomycetes using de novo characterized genetic parts
    • Wang, W., Yang, T., Li, Y., Li, S., Yin, S., Styles, K., Corre, C., Yang, K., Development of a synthetic oxytetracycline-inducible expression system for Streptomycetes using de novo characterized genetic parts. ACS Synth Biol 5 (2016), 765–773.
    • (2016) ACS Synth Biol , vol.5 , pp. 765-773
    • Wang, W.1    Yang, T.2    Li, Y.3    Li, S.4    Yin, S.5    Styles, K.6    Corre, C.7    Yang, K.8
  • 83
    • 85013468584 scopus 로고    scopus 로고
    • Discovery of a phosphonoacetic acid derived natural product by pathway refactoring
    • Freestone, T.S., Ju, K.S., Wang, B., Zhao, H., Discovery of a phosphonoacetic acid derived natural product by pathway refactoring. ACS Synth Biol 6 (2017), 217–223.
    • (2017) ACS Synth Biol , vol.6 , pp. 217-223
    • Freestone, T.S.1    Ju, K.S.2    Wang, B.3    Zhao, H.4
  • 84
    • 85017266739 scopus 로고    scopus 로고
    • CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
    • Heterologous promoters were inserted into Streptomyces using CRISPR-Cas9 to activate silent gene clusters, which triggered production of unique metabolites. The method described here enables the discovery of new small molecules in non-model microbes without the need to refactor and clone gene clusters into a model host.
    • Zhang, M.M., Wong, F.T., Wang, Y., Luo, S., Lim, Y.H., Heng, E., Yeo, W.L., Cobb, R.E., Enghiad, B., Ang, E.L., et al. CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters. Nat Chem Biol 13 (2017), 607–609 Heterologous promoters were inserted into Streptomyces using CRISPR-Cas9 to activate silent gene clusters, which triggered production of unique metabolites. The method described here enables the discovery of new small molecules in non-model microbes without the need to refactor and clone gene clusters into a model host.
    • (2017) Nat Chem Biol , vol.13 , pp. 607-609
    • Zhang, M.M.1    Wong, F.T.2    Wang, Y.3    Luo, S.4    Lim, Y.H.5    Heng, E.6    Yeo, W.L.7    Cobb, R.E.8    Enghiad, B.9    Ang, E.L.10
  • 85
    • 85009727845 scopus 로고    scopus 로고
    • Multiplexed site-specific genome engineering for overproducing bioactive secondary metabolites in actinomycetes
    • Li, L., Zheng, G., Chen, J., Ge, M., Jiang, W., Lu, Y., Multiplexed site-specific genome engineering for overproducing bioactive secondary metabolites in actinomycetes. Metab Eng 40 (2017), 80–92.
    • (2017) Metab Eng , vol.40 , pp. 80-92
    • Li, L.1    Zheng, G.2    Chen, J.3    Ge, M.4    Jiang, W.5    Lu, Y.6
  • 86
    • 84937604736 scopus 로고    scopus 로고
    • A portable expression resource for engineering cross-species genetic circuits and pathways
    • Kushwaha, M., Salis, H.M., A portable expression resource for engineering cross-species genetic circuits and pathways. Nat Commun, 6, 2015, 7832.
    • (2015) Nat Commun , vol.6 , pp. 7832
    • Kushwaha, M.1    Salis, H.M.2
  • 87
    • 85040830753 scopus 로고    scopus 로고
    • Construction and characterization of broad-spectrum promoters for synthetic biology
    • Yang, S., Liu, Q., Zhang, Y., Du, G., Chen, J., Kang, Z., Construction and characterization of broad-spectrum promoters for synthetic biology. ACS Synth Biol 7 (2017), 287–291.
    • (2017) ACS Synth Biol , vol.7 , pp. 287-291
    • Yang, S.1    Liu, Q.2    Zhang, Y.3    Du, G.4    Chen, J.5    Kang, Z.6
  • 88
    • 84935741315 scopus 로고    scopus 로고
    • CRISPR/Cas9-mediated genome engineering of CHO cell factories: application and perspectives
    • Lee, J.S., Grav, L.M., Lewis, N.E., Faustrup Kildegaard, H., CRISPR/Cas9-mediated genome engineering of CHO cell factories: application and perspectives. Biotechnol J 10 (2015), 979–994.
    • (2015) Biotechnol J , vol.10 , pp. 979-994
    • Lee, J.S.1    Grav, L.M.2    Lewis, N.E.3    Faustrup Kildegaard, H.4
  • 89
    • 85027557002 scopus 로고    scopus 로고
    • Enhancing protein production yield from Chinese hamster ovary cells by CRISPR interference
    • Increased copy number of desired genes in CHO cells was achieved through the addition of extra selective pressure with CRISPRi. The use of CRISPRi reduced the number of selection rounds needed to obtain economically relevant cell lines.
    • Shen, C.C., Sung, L.Y., Lin, S.Y., Lin, M.W., Hu, Y.C., Enhancing protein production yield from Chinese hamster ovary cells by CRISPR interference. ACS Synth Biol 6 (2017), 1509–1519 Increased copy number of desired genes in CHO cells was achieved through the addition of extra selective pressure with CRISPRi. The use of CRISPRi reduced the number of selection rounds needed to obtain economically relevant cell lines.
    • (2017) ACS Synth Biol , vol.6 , pp. 1509-1519
    • Shen, C.C.1    Sung, L.Y.2    Lin, S.Y.3    Lin, M.W.4    Hu, Y.C.5
  • 90
    • 85017350101 scopus 로고    scopus 로고
    • Targeted gene deletion using DNA-free RNA-guided Cas9 nuclease accelerates adaptation of CHO cells to suspension culture
    • Using RNA-seq, the authors identified genes in CHO cells that were differentially expressed according to their adaptation trajectory to serum-free media. Genes that were down-regulated were knocked out using CRISPR, effectively accelerating their adaptation to serum-free media, thus shortening time scales of development for industrial CHO cell lines.
    • Lee, N., Shin, J., Park, J.H., Lee, G.M., Cho, S., Cho, B.K., Targeted gene deletion using DNA-free RNA-guided Cas9 nuclease accelerates adaptation of CHO cells to suspension culture. ACS Synth Biol 5 (2016), 1211–1219 Using RNA-seq, the authors identified genes in CHO cells that were differentially expressed according to their adaptation trajectory to serum-free media. Genes that were down-regulated were knocked out using CRISPR, effectively accelerating their adaptation to serum-free media, thus shortening time scales of development for industrial CHO cell lines.
    • (2016) ACS Synth Biol , vol.5 , pp. 1211-1219
    • Lee, N.1    Shin, J.2    Park, J.H.3    Lee, G.M.4    Cho, S.5    Cho, B.K.6
  • 94
    • 84891826628 scopus 로고    scopus 로고
    • CHO cell engineering to prevent polypeptide aggregation and improve therapeutic protein secretion
    • Le Fourn, V., Girod, P.A., Buceta, M., Regamey, A., Mermod, N., CHO cell engineering to prevent polypeptide aggregation and improve therapeutic protein secretion. Metab Eng 21 (2014), 91–102.
    • (2014) Metab Eng , vol.21 , pp. 91-102
    • Le Fourn, V.1    Girod, P.A.2    Buceta, M.3    Regamey, A.4    Mermod, N.5
  • 95
    • 84949575330 scopus 로고    scopus 로고
    • Metabolic engineering of CHO cells to alter lactate metabolism during fed-batch cultures
    • Toussaint, C., Henry, O., Durocher, Y., Metabolic engineering of CHO cells to alter lactate metabolism during fed-batch cultures. J Biotechnol 217 (2016), 122–131.
    • (2016) J Biotechnol , vol.217 , pp. 122-131
    • Toussaint, C.1    Henry, O.2    Durocher, Y.3
  • 98
    • 85007483284 scopus 로고    scopus 로고
    • Predictive glycoengineering of biosimilars using a Markov chain glycosylation model
    • Spahn, P.N., Hansen, A.H., Kol, S., Voldborg, B.G., Lewis, N.E., Predictive glycoengineering of biosimilars using a Markov chain glycosylation model. Biotechnol J, 2017, 12.
    • (2017) Biotechnol J , pp. 12
    • Spahn, P.N.1    Hansen, A.H.2    Kol, S.3    Voldborg, B.G.4    Lewis, N.E.5
  • 99
    • 85010644597 scopus 로고    scopus 로고
    • Integrated genome and protein editing swaps alpha-2,6 sialylation for alpha-2,3 sialic acid on recombinant antibodies from CHO
    • Chung, C.Y., Wang, Q., Yang, S., Yin, B., Zhang, H., Betenbaugh, M., Integrated genome and protein editing swaps alpha-2,6 sialylation for alpha-2,3 sialic acid on recombinant antibodies from CHO. Biotechnol J, 2017, 12.
    • (2017) Biotechnol J , pp. 12
    • Chung, C.Y.1    Wang, Q.2    Yang, S.3    Yin, B.4    Zhang, H.5    Betenbaugh, M.6
  • 100
    • 84976352030 scopus 로고    scopus 로고
    • Immunization with outer membrane vesicles displaying designer glycotopes yields class-switched, glycan-specific antibodies
    • The authors created a versatile and efficient platform for generation of specific and on-demand immunogens. These immunostimulatory glycoconjugates were produced on the surface of E. coli, which could be exposed to an organism of choice to induce a glycan-specific antibody response.
    • Valentine, J.L., Chen, L., Perregaux, E.C., Weyant, K.B., Rosenthal, J.A., Heiss, C., Azadi, P., Fisher, A.C., Putnam, D., Moe, G.R., et al. Immunization with outer membrane vesicles displaying designer glycotopes yields class-switched, glycan-specific antibodies. Cell Chem Biol 23 (2016), 655–665 The authors created a versatile and efficient platform for generation of specific and on-demand immunogens. These immunostimulatory glycoconjugates were produced on the surface of E. coli, which could be exposed to an organism of choice to induce a glycan-specific antibody response.
    • (2016) Cell Chem Biol , vol.23 , pp. 655-665
    • Valentine, J.L.1    Chen, L.2    Perregaux, E.C.3    Weyant, K.B.4    Rosenthal, J.A.5    Heiss, C.6    Azadi, P.7    Fisher, A.C.8    Putnam, D.9    Moe, G.R.10
  • 101
    • 84979790855 scopus 로고    scopus 로고
    • Identifying and retargeting transcriptional hot spots in the human genome
    • Cheng, J.K., Lewis, A.M., Kim do, S., Dyess, T., Alper, H.S., Identifying and retargeting transcriptional hot spots in the human genome. Biotechnol J 11 (2016), 1100–1109.
    • (2016) Biotechnol J , vol.11 , pp. 1100-1109
    • Cheng, J.K.1    Lewis, A.M.2    Kim do, S.3    Dyess, T.4    Alper, H.S.5
  • 102
    • 85006380356 scopus 로고    scopus 로고
    • Transcriptomics-guided design of synthetic promoters for a mammalian system
    • Cheng, J.K., Alper, H.S., Transcriptomics-guided design of synthetic promoters for a mammalian system. ACS Synth Biol 5 (2016), 1455–1465.
    • (2016) ACS Synth Biol , vol.5 , pp. 1455-1465
    • Cheng, J.K.1    Alper, H.S.2
  • 103
    • 85024409143 scopus 로고    scopus 로고
    • Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding
    • Lan, F., Demaree, B., Ahmed, N., Abate, A.R., Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding. Nat Biotechnol 35 (2017), 640–646.
    • (2017) Nat Biotechnol , vol.35 , pp. 640-646
    • Lan, F.1    Demaree, B.2    Ahmed, N.3    Abate, A.R.4
  • 104
    • 84966334405 scopus 로고    scopus 로고
    • The all E. coli TX–TL toolbox 2.0: a platform for cell-free synthetic biology
    • Garamella, J., Marshall, R., Rustad, M., Noireaux, V., The all E. coli TX–TL toolbox 2.0: a platform for cell-free synthetic biology. ACS Synth Biol 5 (2016), 344–355.
    • (2016) ACS Synth Biol , vol.5 , pp. 344-355
    • Garamella, J.1    Marshall, R.2    Rustad, M.3    Noireaux, V.4
  • 105
    • 85013662404 scopus 로고    scopus 로고
    • Streptomyces venezuelae TX–TL — a next generation cell-free synthetic biology tool
    • Moore, S.J., Lai, H.E., Needham, H., Polizzi, K.M., Freemont, P.S., Streptomyces venezuelae TX–TL — a next generation cell-free synthetic biology tool. Biotechnol J, 2017, 12.
    • (2017) Biotechnol J , pp. 12
    • Moore, S.J.1    Lai, H.E.2    Needham, H.3    Polizzi, K.M.4    Freemont, P.S.5
  • 106
    • 84944345469 scopus 로고    scopus 로고
    • Harnessing the landscape of microbial culture media to predict new organism-media pairings
    • Oberhardt, M.A., Zarecki, R., Gronow, S., Lang, E., Klenk, H.P., Gophna, U., Ruppin, E., Harnessing the landscape of microbial culture media to predict new organism-media pairings. Nat Commun, 6, 2015, 8493.
    • (2015) Nat Commun , vol.6 , pp. 8493
    • Oberhardt, M.A.1    Zarecki, R.2    Gronow, S.3    Lang, E.4    Klenk, H.P.5    Gophna, U.6    Ruppin, E.7
  • 107
    • 85015175359 scopus 로고    scopus 로고
    • Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve biosimilar product quality
    • Bruhlmann, D., Sokolov, M., Butte, A., Sauer, M., Hemberger, J., Souquet, J., Broly, H., Jordan, M., Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve biosimilar product quality. Biotechnol Bioeng 114 (2017), 1448–1458.
    • (2017) Biotechnol Bioeng , vol.114 , pp. 1448-1458
    • Bruhlmann, D.1    Sokolov, M.2    Butte, A.3    Sauer, M.4    Hemberger, J.5    Souquet, J.6    Broly, H.7    Jordan, M.8
  • 108
    • 85016589864 scopus 로고    scopus 로고
    • A model for designing adaptive laboratory evolution experiments
    • The authors developed a computational model based on adaptive laboratory evolution that was designed to balance desired industrial phenotypes with available laboratory resources. Their model provides a standard procedure for quantifying and performing adaptive laboratory evolution experiments, limiting the inefficient use of resources.
    • LaCroix, R.A., Palsson, B.O., Feist, A.M., A model for designing adaptive laboratory evolution experiments. Appl Environ Microbiol, 2017, 83 The authors developed a computational model based on adaptive laboratory evolution that was designed to balance desired industrial phenotypes with available laboratory resources. Their model provides a standard procedure for quantifying and performing adaptive laboratory evolution experiments, limiting the inefficient use of resources.
    • (2017) Appl Environ Microbiol , pp. 83
    • LaCroix, R.A.1    Palsson, B.O.2    Feist, A.M.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.