Multiplexed site-specific genome engineering for overproducing bioactive secondary metabolites in actinomycetes

Metabolic Engineering

Volumn 40, Issue , 2017, Pages 80-92

  Li, Lei ^a,b   Zheng, Guosong ^a   Chen, Jun ^a   Ge, Mei ^c   Jiang, Weihong ^a,d   Lu, Yinhua ^a,e  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c Shanghai Laiyi Center for Biopharmaceuticals RandD   (China)

^d Jiangsu National Synergetic Innovation Center for Advanced Materials   (China)

^e SHANGHAI COLLABORATIVE INNOVATION CENTER FOR BIOMANUFACTURING TECHNOLOGY   (China)

Author keywords

Actinomycetes; Gene cluster amplification; Genome mining; Secondary metabolite; Site specific recombination

Indexed keywords

BACTERIA; BIOMOLECULES; GENES; MULTIPLEXING;

ACTINOMYCETES; GENE CLUSTERS; GENOME MINING; SECONDARY METABOLITES; SITE-SPECIFIC RECOMBINATION;

METABOLITES;

ANTINEOPLASTIC AGENT; CHLORAMPHENICOL; NATURAL PRODUCT; PRISTINAMYCIN; SECONDARY METABOLITE; UNCLASSIFIED DRUG; YM 216391; CYCLOPEPTIDE; OXAZOLE DERIVATIVE;

ARTICLE; ATTB GENE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; BATCH FERMENTATION; CONTROLLED STUDY; CRISPR CAS SYSTEM; DRUG MANUFACTURE; FERMENTATION; GENE AMPLIFICATION; GENE CLUSTER; GENE EDITING; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC STABILITY; IN VITRO STUDY; MULTIPLEXED SITE SPECIFIC GENOME ENGINEERING; NONHUMAN; PRIORITY JOURNAL; STREPTOMYCES; STREPTOMYCES COELICOLOR; STREPTOMYCES PRISTINAESPIRALIS; BACTERIAL GENOME; BIOSYNTHESIS; GENETIC ENHANCEMENT; GENETICS; ISOLATION AND PURIFICATION; METABOLIC ENGINEERING; METABOLISM; MULTIGENE FAMILY; PHYSIOLOGY; PROCEDURES; SECONDARY METABOLISM; UPREGULATION;

BIOSYNTHETIC PATHWAYS; CHLORAMPHENICOL; GENETIC ENHANCEMENT; GENOME, BACTERIAL; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; MULTIGENE FAMILY; OXAZOLES; PEPTIDES, CYCLIC; SECONDARY METABOLISM; STREPTOMYCES; UP-REGULATION;

EID: 85009727845     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2017.01.004     Document Type: Article

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