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Volumn 35, Issue 7, 2017, Pages 640-646

Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELL CULTURE; CELL PROLIFERATION; CELLS; DROPS; GENE ENCODING; GENES; MICROFLUIDICS; SILICON CARBIDE;

EID: 85024409143     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3880     Document Type: Article
Times cited : (323)

References (27)
  • 2
    • 0028982462 scopus 로고
    • Viral abundance in aquatic systems: A comparison between marine and fresh waters
    • Maranger, R. & Bird, D. Viral abundance in aquatic systems: a comparison between marine and fresh waters. Mar. Ecol. Prog. Ser. 121, 217-226 (1995).
    • (1995) Mar. Ecol. Prog. Ser. , vol.121 , pp. 217-226
    • Maranger, R.1    Bird, D.2
  • 3
    • 45849138873 scopus 로고    scopus 로고
    • Optical tweezers for single cells
    • Zhang, H. & Liu, K.-K. Optical tweezers for single cells. J. R. Soc. Interface 5, 671-690 (2008).
    • (2008) J. R. Soc. Interface , vol.5 , pp. 671-690
    • Zhang, H.1    Liu, K.-K.2
  • 4
    • 84899507119 scopus 로고    scopus 로고
    • Obtaining genomes from uncultivated environmental microorganisms using FACS-based single-cell genomics
    • Rinke, C. et al. Obtaining genomes from uncultivated environmental microorganisms using FACS-based single-cell genomics. Nat. Protoc. 9, 1038-1048 (2014).
    • (2014) Nat. Protoc. , vol.9 , pp. 1038-1048
    • Rinke, C.1
  • 5
    • 84919372228 scopus 로고    scopus 로고
    • Dissecting the clonal origins of childhood acute lymphoblastic leukemia by single-cell genomics
    • Gawad, C., Koh, W. & Quake, S.R. Dissecting the clonal origins of childhood acute lymphoblastic leukemia by single-cell genomics. Proc. Natl. Acad. Sci. USA 111, 17947-17952 (2014).
    • (2014) Proc. Natl. Acad. Sci. USA , vol.111 , pp. 17947-17952
    • Gawad, C.1    Koh, W.2    Quake, S.R.3
  • 6
    • 84979547050 scopus 로고    scopus 로고
    • Robust high-performance nanoliter-volume single-cell multiple displacement amplification on planar substrates
    • Leung, K. et al. Robust high-performance nanoliter-volume single-cell multiple displacement amplification on planar substrates. Proc. Natl. Acad. Sci. USA 113, 8484-8489 (2016).
    • (2016) Proc. Natl. Acad. Sci. USA , vol.113 , pp. 8484-8489
    • Leung, K.1
  • 7
    • 84927125749 scopus 로고    scopus 로고
    • Single gene-based distinction of individual microbial genomes from a mixed population of microbial cells
    • Tamminen, M.V. & Virta, M.P.J. Single gene-based distinction of individual microbial genomes from a mixed population of microbial cells. Front. Microbiol. 6, 195 (2015).
    • (2015) Front. Microbiol. , vol.6 , pp. 195
    • Tamminen, M.V.1    Virta, M.P.J.2
  • 8
    • 34249701816 scopus 로고    scopus 로고
    • Targeted access to the genomes of low-abundance organisms in complex microbial communities
    • Podar, M. et al. Targeted access to the genomes of low-abundance organisms in complex microbial communities. Appl. Environ. Microbiol. 73, 3205-3214 (2007).
    • (2007) Appl. Environ. Microbiol. , vol.73 , pp. 3205-3214
    • Podar, M.1
  • 9
    • 84980398168 scopus 로고    scopus 로고
    • Virtual microfluidics for digital quantification and single-cell sequencing
    • Xu, L., Brito, I.L., Alm, E.J. & Blainey, P.C. Virtual microfluidics for digital quantification and single-cell sequencing. Nat. Methods 13, 759-762 (2016).
    • (2016) Nat. Methods , vol.13 , pp. 759-762
    • Xu, L.1    Brito, I.L.2    Alm, E.J.3    Blainey, P.C.4
  • 10
    • 84976609584 scopus 로고    scopus 로고
    • Droplet barcoding for massively parallel single-molecule deep sequencing
    • Lan, F., Haliburton, J.R., Yuan, A. & Abate, A.R. Droplet barcoding for massively parallel single-molecule deep sequencing. Nat. Commun. 7, 11784 (2016).
    • (2016) Nat. Commun. , vol.7 , pp. 11784
    • Lan, F.1    Haliburton, J.R.2    Yuan, A.3    Abate, A.R.4
  • 11
    • 84946545109 scopus 로고    scopus 로고
    • Single-cell ChIP-seq reveals cell subpopulations defined by chromatin state
    • Rotem, A. et al. Single-cell ChIP-seq reveals cell subpopulations defined by chromatin state. Nat. Biotechnol. 33, 1165-1172 (2015).
    • (2015) Nat. Biotechnol. , vol.33 , pp. 1165-1172
    • Rotem, A.1
  • 12
    • 84929684998 scopus 로고    scopus 로고
    • Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells
    • Klein, A.M. et al. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell 161, 1187-1201 (2015).
    • (2015) Cell , vol.161 , pp. 1187-1201
    • Klein, A.M.1
  • 13
    • 84929684999 scopus 로고    scopus 로고
    • Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets
    • Macosko, E.Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 161, 1202-1214 (2015).
    • (2015) Cell , vol.161 , pp. 1202-1214
    • Macosko, E.Z.1
  • 14
    • 84930685084 scopus 로고    scopus 로고
    • High-throughput single-cell labeling (Hi-SCL) for RNA-seq using drop-based microfluidics
    • Rotem, A. et al. High-throughput single-cell labeling (Hi-SCL) for RNA-seq using drop-based microfluidics. PLoS One 10, e0116328 (2015).
    • (2015) PLoS One , vol.10 , pp. e0116328
    • Rotem, A.1
  • 15
    • 78650879877 scopus 로고    scopus 로고
    • Single-cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions
    • Novak, R. et al. Single-cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions. Angew. Chem. Int. Edn Engl. 50, 390-395 (2011).
    • (2011) Angew. Chem. Int. Edn Engl. , vol.50 , pp. 390-395
    • Novak, R.1
  • 16
    • 7544247824 scopus 로고    scopus 로고
    • Formation of monodisperse bubbles in a microfluidic flowfocusing device
    • Garstecki, P. et al. Formation of monodisperse bubbles in a microfluidic flowfocusing device. Appl. Phys. Lett. 85, 2649-2651 (2004).
    • (2004) Appl. Phys. Lett. , vol.85 , pp. 2649-2651
    • Garstecki, P.1
  • 17
    • 84922537404 scopus 로고    scopus 로고
    • Haplotype-resolved whole-genome sequencing by contiguitypreserving transposition and combinatorial indexing
    • Amini, S. et al. Haplotype-resolved whole-genome sequencing by contiguitypreserving transposition and combinatorial indexing. Nat. Genet. 46, 1343-1349 (2014).
    • (2014) Nat. Genet. , vol.46 , pp. 1343-1349
    • Amini, S.1
  • 18
    • 84920645442 scopus 로고    scopus 로고
    • A quantitative comparison of single-cell whole genome amplification methods
    • de Bourcy, C.F.A. et al. A quantitative comparison of single-cell whole genome amplification methods. PLoS One 9, e105585 (2014).
    • (2014) PLoS One , vol.9 , pp. e105585
    • De Bourcy, C.F.A.1
  • 19
    • 84890055556 scopus 로고    scopus 로고
    • Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells
    • Gole, J. et al. Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells. Nat. Biotechnol. 31, 1126-1132 (2013).
    • (2013) Nat. Biotechnol. , vol.31 , pp. 1126-1132
    • Gole, J.1
  • 20
    • 84908577353 scopus 로고    scopus 로고
    • Antibiotic resistance-problems, progress, and prospects
    • Nathan, C. & Cars, O. Antibiotic resistance-problems, progress, and prospects. N. Engl. J. Med. 371, 1761-1763 (2014).
    • (2014) N. Engl. J. Med. , vol.371 , pp. 1761-1763
    • Nathan, C.1    Cars, O.2
  • 21
    • 33947592971 scopus 로고    scopus 로고
    • Processes controlling the transmission of bacterial pathogens in the environment
    • Yildiz, F.H. Processes controlling the transmission of bacterial pathogens in the environment. Res. Microbiol. 158, 195-202 (2007).
    • (2007) Res. Microbiol. , vol.158 , pp. 195-202
    • Yildiz, F.H.1
  • 22
    • 0031902076 scopus 로고    scopus 로고
    • Gene transfer by transduction in the marine environment
    • Jiang, S.C. & Paul, J.H. Gene transfer by transduction in the marine environment. Appl. Environ. Microbiol. 64, 2780-2787 (1998).
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 2780-2787
    • Jiang, S.C.1    Paul, J.H.2
  • 23
    • 0035843908 scopus 로고    scopus 로고
    • Genes lost and genes found: Evolution of bacterial pathogenesis and symbiosis
    • Ochman, H. & Moran, N.A. Genes lost and genes found: evolution of bacterial pathogenesis and symbiosis. Science 292, 1096-1099 (2001).
    • (2001) Science , vol.292 , pp. 1096-1099
    • Ochman, H.1    Moran, N.A.2
  • 24
    • 84862276328 scopus 로고    scopus 로고
    • Structure, function and diversity of the healthy human microbiome
    • Human Microbiome Project Consortium
    • Human Microbiome Project Consortium. Structure, function and diversity of the healthy human microbiome. Nature 486, 207-214 (2012).
    • (2012) Nature , vol.486 , pp. 207-214
  • 25
    • 84925104897 scopus 로고    scopus 로고
    • Geospatial resolution of human and bacterial diversity with city-scale metagenomics
    • Afshinnekoo, E. et al. Geospatial resolution of human and bacterial diversity with city-scale metagenomics. Cell Syst. 1, 72-87 (2015).
    • (2015) Cell Syst. , vol.1 , pp. 72-87
    • Afshinnekoo, E.1
  • 26
    • 84856659328 scopus 로고    scopus 로고
    • Untangling genomes from metagenomes: Revealing an uncultured class of marine Euryarchaeota
    • Iverson, V. et al. Untangling genomes from metagenomes: revealing an uncultured class of marine Euryarchaeota. Science 335, 587-590 (2012).
    • (2012) Science , vol.335 , pp. 587-590
    • Iverson, V.1
  • 27
    • 84891354380 scopus 로고    scopus 로고
    • Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients
    • Ni, X. et al. Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients. Proc. Natl. Acad. Sci. USA 110, 21083-21088 (2013).
    • (2013) Proc. Natl. Acad. Sci. USA , vol.110 , pp. 21083-21088
    • Ni, X.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.