CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Ronda, Carlotta ^a   Maury, Jérôme ^a   Jakočiunas, Tadas ^a   Baallal Jacobsen, Simo Abdessamad ^a   Germann, Susanne Manuela ^a   Harrison, Scott James ^a   Borodina, Irina ^a   Keasling, Jay D ^a   Jensen, Michael Krogh ^a   Nielsen, Alex Toftgaard ^a  

^a Chalmers University of Technology   (Denmark)

Author keywords

Carotenoid production; CRISPR Cas9; Genome editing; Genome integrations; Metabolic engineering; Saccharomyces cerevisiae

Indexed keywords

BETA CAROTENE; DNA FRAGMENT; GENOMIC DNA; HIGH MOBILITY GROUP B1 PROTEIN; MEVALONIC ACID; RNA;

ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; COPY NUMBER VARIATION; COST EFFECTIVENESS ANALYSIS; DNA INTEGRATION; DNA MARKER; DOUBLE STRANDED DNA BREAK; FUNGAL GENETICS; GENE CASSETTE; GENE EXPRESSION; GENE LOCUS; HOMOLOGOUS RECOMBINATION; MULTILOCUS SEQUENCE TYPING; NONHUMAN; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; GENE VECTOR; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

SACCHAROMYCES CEREVISIAE;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENE EXPRESSION; GENETIC VECTORS; METABOLIC ENGINEERING; SACCHAROMYCES CEREVISIAE;

EID: 84935426318     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0288-3     Document Type: Article

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