CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives

Biotechnology Journal

Volumn 10, Issue 7, 2015, Pages 979-994

  Lee, Jae Seong ^a   Grav, Lise Marie ^a   Lewis, Nathan E ^b,c   Kildegaard, Helene Faustrup ^a  

^a Chalmers University of Technology   (Denmark)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cell factories; Cell line engineering; Chinese hamster ovary cells; CRISPR Cas9; Genome editing

Indexed keywords

CELL CULTURE; CELLS; CYTOLOGY; GENES; MAMMALS; PROTEINS;

CELL LINES; CHINESE HAMSTER OVARY CELLS; CRISPR/CAS9; FUTURE PERSPECTIVES; GENOME ENGINEERING; HIGH-LEVEL PRODUCTION; QUALITY ATTRIBUTES; THERAPEUTIC PROTEIN;

CELL ENGINEERING;

ADAPTIVE IMMUNITY; CHO CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA MODIFICATION; DNA REPAIR; DNA TEMPLATE; GENE ACTIVATION; GENE DISRUPTION; GENE IDENTIFICATION; GENE REPRESSION; GENE TARGETING; GENETIC ASSOCIATION; GENETIC ENGINEERING; HUMAN; MAMMAL CELL; PRIORITY JOURNAL; REVIEW; SYSTEMS BIOLOGY; ANIMAL; CELL ENGINEERING; CRICETULUS; CRISPR CAS SYSTEM; GENOME; HAMSTER;

BACTERIA (MICROORGANISMS); CRICETULUS GRISEUS; MAMMALIA;

ANIMALS; CELL ENGINEERING; CHO CELLS; CRICETINAE; CRICETULUS; CRISPR-CAS SYSTEMS; GENOME; HUMANS;

EID: 84935741315     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500082     Document Type: Review

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