Functional optimization of gene clusters by combinatorial design and assembly

Nature Biotechnology

Volumn 32, Issue 12, 2014, Pages 1241-1249

  Smanski, Michael J ^a   Bhatia, Swapnil ^b   Zhao, Dehua ^a   Park, YongJin ^a   Woodruff, Lauren B A ^a,c   Giannoukos, Georgia ^c   Ciulla, Dawn ^c   Busby, Michele ^c   Calderon, Johnathan ^a   Nicol, Robert ^c   Gordon, D Benjamin ^a,c   Densmore, Douglas ^b   Voigt, Christopher A ^a,c  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b Boston University   (United States)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOCHEMISTRY; DESIGN; ENERGY UTILIZATION; NITROGEN FIXATION; NUCLEIC ACIDS; RNA;

CLUSTER ARCHITECTURE; COMBINATORIAL DESIGN; COMPLEX REGULATION; FUNCTIONAL OPTIMIZATION; GENETIC MANIPULATIONS; GENETIC OPTIMIZATION; HYPOTHESIS TESTING; KLEBSIELLA OXYTOCA;

GENES;

ARTICLE; COMBINATORIAL CHEMISTRY; COMBINATORIAL DESIGN; DNA ASSEMBLY; DNA FLANKING REGION; GENE CLUSTER; GENE ORDER; GENETIC MANIPULATION; GENETIC VARIABILITY; KLEBSIELLA OXYTOCA; NITROGEN FIXATION; NONHUMAN; OPEN READING FRAME; OPERON; PRIORITY JOURNAL; PROMOTER REGION; RNA SEQUENCE; TRANSCRIPTOMICS; GENETIC TRANSCRIPTION; GENETICS; HIGH THROUGHPUT SEQUENCING; MULTIGENE FAMILY; PHYSIOLOGY; PROCEDURES;

KLEBSIELLA OXYTOCA;

NITROGENASE;

HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; KLEBSIELLA OXYTOCA; MULTIGENE FAMILY; NITROGEN FIXATION; NITROGENASE; OPERON; PROMOTER REGIONS, GENETIC; TRANSCRIPTION, GENETIC;

EID: 84921517479     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3063     Document Type: Article

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