Targeted Gene Deletion Using DNA-Free RNA-Guided Cas9 Nuclease Accelerates Adaptation of CHO Cells to Suspension Culture

ACS Synthetic Biology

Volumn 5, Issue 11, 2016, Pages 1211-1219

  Lee, Namil ^a   Shin, Jongoh ^a   Park, Jin Hyoung ^a   Lee, Gyun Min ^a   Cho, Suhyung ^a   Cho, Byung Kwan ^a,b  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Intelligent Synthetic Biology Center   (South Korea)

Author keywords

Chinese hamster ovary (CHO) cells; DNA free CRISPR Cas9; genome editing; high throughput RNA seq; suspension culture

Indexed keywords

CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN 9; UNCLASSIFIED DRUG; GUIDE RNA;

ANIMAL CELL; ARTICLE; CHO CELL LINE; GENE DELETION; GENE EDITING; HIGH THROUGHPUT SEQUENCING; NONHUMAN; PRIORITY JOURNAL; RNA SEQUENCE; SERUM-FREE MEDIUM; SUSPENSION CELL CULTURE; ANIMAL; CRICETULUS; CRISPR CAS SYSTEM; GENETICS; GENOMICS; PROTEIN ENGINEERING; SEQUENCE ANALYSIS;

ANIMALS; CHO CELLS; CRICETULUS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; GENE DELETION; GENOMICS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; PROTEIN ENGINEERING; RNA, GUIDE; SEQUENCE ANALYSIS, RNA;

EID: 85017350101     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.5b00249     Document Type: Article

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