Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors

Nature Methods

Volumn 14, Issue 12, 2017, Pages 1163-1166

  Tak, Y Esther ^a,b   Kleinstiver, Benjamin P ^a,b   Nuñez, James K ^c   Hsu, Jonathan Y ^a,d   Horng, Joy E ^a   Gong, Jingyi ^a   Weissman, Jonathan S ^c,e   Joung, J Keith ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d Massachusetts Institute of Technology   (United States)

^e HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CPF1 NUCLEASE; NUCLEASE; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; BRLF1 PROTEIN, HUMAN HERPESVIRUS 4; ENDONUCLEASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; IMMEDIATE EARLY PROTEIN; TRANSACTIVATOR PROTEIN; TRANSCRIPTION FACTOR RELA;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE FUSION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DOMAIN; TRANSCRIPTION INITIATION; CELL CULTURE TECHNIQUE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; GENETIC TRANSFECTION; GENETICS; HEK293 CELL LINE; PLASMID;

BACTERIAL PROTEINS; CELL CULTURE TECHNIQUES; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; ENDONUCLEASES; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HERPES SIMPLEX VIRUS PROTEIN VMW65; HUMANS; IMMEDIATE-EARLY PROTEINS; PLASMIDS; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATORS; TRANSCRIPTION FACTOR RELA; TRANSCRIPTIONAL ACTIVATION; TRANSFECTION;

EID: 85036644868     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.4483     Document Type: Article

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