PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease

Cell

Volumn 167, Issue 7, 2016, Pages 1814-1828.e12

  Yang, Hui ^a   Gao, Pu ^a,b   Rajashankar, Kanagalaghatta R ^c,d   Patel, Dinshaw J ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c Cornell University ^*  (United States)

^d ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

binary complex with sgRNA; C2c1; genome editing tool; RuvC catalytic pocket; sequence specific PAM recognition; structure; ternary complex with added DNA; type V CRISPR Cas endonuclease

Indexed keywords

BACTERIAL ENZYME; C2C1 PROTEIN; DNA; ENDONUCLEASE; GUIDE RNA; HETERODUPLEX; HYDROGEN; OXYGEN; PHOSPHATE; TRYPTOPHAN; UNCLASSIFIED DRUG; DEOXYRIBONUCLEASE; EMPTY SPIRACLES HOMEOBOX PROTEINS; HOMEODOMAIN PROTEIN; TRANSCRIPTION FACTOR; UNTRANSLATED RNA;

ALICYCLOBACILLUS; ALPHA HELIX; ARTICLE; BETA SHEET; BIOINFORMATICS; CATALYSIS; COMPLEX FORMATION; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRYSTAL STRUCTURE; DNA STRAND; ENZYME CONFORMATION; HYDROGEN BOND; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; CLASSIFICATION; ENZYMOLOGY; GENE EDITING; GENETICS; HUMAN; METABOLISM; MOLECULAR MODEL; X RAY CRYSTALLOGRAPHY;

ALICYCLOBACILLUS; CRISPR-CAS SYSTEMS; CRYSTALLOGRAPHY, X-RAY; ENDODEOXYRIBONUCLEASES; GENE EDITING; HOMEODOMAIN PROTEINS; HUMANS; MODELS, MOLECULAR; RNA, UNTRANSLATED; TRANSCRIPTION FACTORS;

EID: 85006511857     PISSN: 00928674     EISSN: 10974172     Source Type: Journal    
DOI: 10.1016/j.cell.2016.11.053     Document Type: Article

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