CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

Microbial Cell Factories

Volumn 16, Issue 1, 2017, Pages

  Cress, Brady F ^a   Leitz, Quentin D ^a   Kim, Daniel C ^a   Amore, Teresita D ^b   Suzuki, Jon Y ^c   Linhardt, Robert J ^a,d   Koffas, Mattheos A G ^a,d  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b UNIVERSITY OF HAWAII   (United States)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

^d Research Rensselaer Polytechnic Institute   (United States)

Author keywords

AdoMet; Anthocyanin O methyltransferase; CRISPRi; DCas9; Deregulation; MetJ; Peonidin 3 O glucoside; S Adenosyl methionine; SAM; Transcriptional repression

Indexed keywords

ANTHOCYANIDIN 3 O GLUCOSYLTRANSFERASE; ANTHOCYANIDIN SYNTHASE; ANTHOCYANIN; ANTHOCYANIN O METHYLTRANSFERASE; CATECHIN; FLAVONOL; GLUCOSIDE; GLUCOSYLTRANSFERASE; METHYLTRANSFERASE; PEONIDIN 3 O GLUCOSIDE; S ADENOSYLMETHIONINE; SYNTHETASE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GLUCOSE; GLUCOSE; PEONIDIN 3-GLUCOSIDE; PLANT PROTEIN;

ARABIDOPSIS THALIANA; ARTICLE; BACTERIAL STRAIN; BIOCHEMICAL COMPOSITION; BIOSYNTHESIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; ESCHERICHIA COLI; GENE DOSAGE; GENE EXPRESSION; GENE SILENCING; GRAPE; METABOLIC ENGINEERING; METJ GENE; NONHUMAN; PETUNIA; PETUNIA HYBRIDA; PLANT GENE; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; CHEMISTRY; CRISPR CAS SYSTEM; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

ANTHOCYANINS; BIOSYNTHETIC PATHWAYS; CATECHIN; CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; GLUCOSE; GLUCOSIDES; METABOLIC ENGINEERING; METHYLTRANSFERASES; PETUNIA; PLANT PROTEINS; S-ADENOSYLMETHIONINE;

EID: 85011320204     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0623-3     Document Type: Article

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