Development of a Synthetic Oxytetracycline-Inducible Expression System for Streptomycetes Using de Novo Characterized Genetic Parts

ACS Synthetic Biology

Volumn 5, Issue 7, 2016, Pages 765-773

  Wang, Weishan ^a   Yang, Tongjian ^a,b   Li, Yihong ^a   Li, Shanshan ^a   Yin, Shouliang ^a   Styles, Kathryn ^c   Corre, Christophe ^c   Yang, Keqian ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

biosynthetic gene cluster; genetic parts; inducible expression system; natural product; streptomycetes; wide range strength

Indexed keywords

BACTERIAL PROTEIN; DEOXYRIBONUCLEASE I; JADOMYCIN; OXYTETRACYCLINE; POLYKETIDE; UNCLASSIFIED DRUG; ISOQUINOLINE DERIVATIVE; JADOMYCIN B;

ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL GENOME; BIOLUMINESCENCE; CONTROLLED STUDY; DISSOCIATION; DISSOCIATION CONSTANT; DNA PROTEIN COMPLEX; ESCHERICHIA COLI; GEL MOBILITY SHIFT ASSAY; GENE CLUSTER; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; IN VITRO STUDY; NONHUMAN; OPERATOR OTRO; OXYTETRACYCLINE RESPONSIVE REPRESSOR OTRR; PRIORITY JOURNAL; PROMOTER OTRB; PROMOTER REGION; PROTEIN EXPRESSION; REGULATOR GENE; REPORTER GENE; STREPTOMYCES ALBUS; STREPTOMYCES COELICOLOR; STREPTOMYCES RIMOSUS; STREPTOMYCES VENEZUELAE; STREPTOMYCETACEAE; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION; DRUG EFFECTS; GENETICS; METABOLISM; MULTIGENE FAMILY; STREPTOMYCES;

BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GENOME, BACTERIAL; ISOQUINOLINES; MULTIGENE FAMILY; OXYTETRACYCLINE; POLYKETIDES; PROMOTER REGIONS, GENETIC; STREPTOMYCES;

EID: 84978712445     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00087     Document Type: Article

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